51results about How to "Suitable for mass inspection" patented technology

The invention discloses a primer combination for identifying ChPV (Chicken Parvovirus) and ARV (Avian Reoviruses) and application of the primer combination. The primer combination consists of a primer pair I and a primer pair II, wherein the primer pair I consists of ChPV-F and ChPV-R which are respectively shown in sequence tables 1 and 2; the primer pair II consists of ARV-F and ARV-R which are respectively shown in sequence tables 3 and 4. The invention protects the application of the primer combination in identifying the ChPV and the ARV, the application of the primer combination in identifying whether a to-be-detected virus is ChPV or ARV or not and the application of the primer combination in identifying whether a to-be-detected sample is infected with the ChPV and/or the ARV or not. According to the primer combination for identifying the ChPV and the ARV and the application of the primer combination, disclosed by the invention, a duplex PCR (Polymerase Chain Reaction) established by the invention can be used for rapidly detecting mixed infection of the ChPV and the ARV and is suitable for large-batch detection, the cost and the time are saved, pollution is also reduced, and very high practical value is obtained.

The invention discloses a multi-PCR detection kit for identifying poultry salmonella. The multi-PCR detection kit comprises PCR buffer fluid, a deoxyribonucleoside triphosphate mixture, Taq DNA polymerase and primer pairs of a bcf gene, a heli gene, an rhs gene, an sdf gene, a fla gene. The invention further discloses a multi-PCR detection method for identifying the poultry salmonella through the kit. The multi-PCR detection kit can effectively determine whether the salmonella is included in a sample to be detected or not, and can further identify different epidemic salmonella serotypes in poultry production or poultry products. Through a PCR, the salmonella can be detected from the sample at the same time, the different salmonella serotypes are further identified, the detection reaction sensitivity is high, high specificity is achieved, the detection process is fast and efficient, and the kit and method are suitable for batch detection.

The invention relates to a separation and detection method for astaxanthin in a haematococcus pluvialis extract. The separation and detection method comprises the following steps of 1 extraction of carotenoid in the haematococcus pluvialis extract; 2 enzymolysis of the carotenoid; 3 astaxanthin separation and detection through a normal-phase high performance liquid analysis method, wherein the liquid-phase on-device conditions comprise the detection wavelength is 474 nm, a chromatographic column is Luna 3micro Silica(2), the chromatographic column temperature ranges from 20 DEG C to 25 DEG C, the flow speed is 1-1.2 ml/min, a flow phase is prepared from n-hexane and acetone according to the volume ratio of 75%:25%-90%:10%, and isocratic elution is conducted. According to the separation and detection method, the pretreatment efficiency is high, the intersolubility between the extracting reagent acetone and the astaxanthin is good, the enzymolysis time is shorter than the saponification time, and the efficiency is high; the high performance liquid chromatography on-device conditions are good; isocratic elution is achieved, a base line is easier to stabilize, the peak shape is good, the separation degree is high, and more isomers can be separated out; the peak flowing time is short, and the method is not prone to be influenced by outside light and heat and more suitable for large-scale detection.

The invention provides a method for quantitatively determining sympathatic detonation of an explosive, and belongs to the technical field of blasting. The method comprises the following steps: fixinga continuous resistance wire probe to centers of a detonating cartridge and a detonated cartridge, integrally placing the detonating cartridge and the detonated cartridge with the continuous resistance wire probe in a semicircular pipe groove of a test support, and keeping the cartridges in the same axis; regulating the sympathetic detonation distance to be in preset value L by moving the detonated cartridge, and connecting a probe led out from the tail end of the detonated cartridge to a signal acquisition instrument through a coaxial cable; inserting a detonator into a detonating end of thedetonating cartridge, starting the signal acquisition instrument, detonating the explosive, and recording a voltage signal curve of the continuous resistance wire probe by the signal acquisition instrument; converting, thus obtaining a detonation wave-impact wave time travel curve; comparing a detonation wave travel with the length of the cartridges, thus judging whether the detonated cartridge isin sympathatic detonation or not. A measurement system is simple, the cost is lower, a result is direct and reliable and is basically not affected by a testing environment, and the explosive amount is not limited.

The invention discloses a method for detecting potential pollution risk of aflatoxin in peanuts and products thereof by using real-time fluorescent quantitative PCR (polymerase chain reaction) technology. The method utilizes a quartering process to collect the samples, utilizes a benzyl chloride process to extract the DNA (deoxyribonucleic acid), and designs the specific primer by using the Aspergillus flavus toxin production key gene AFLR as the target gene to perform the fluorescent quantitative PCR, judges the aflatoxin pollution risk of the sample according to the fluorescent quantitative Ct value, and establishes a relation model between the aflatoxin pollution risk and sample fluorescent quantitative Ct value. The method can quickly and accurately judge the potential pollution risk of aflatoxin in the peanuts and products thereof, has the advantages of high specificity, high stability and high sensitivity, is simple and quick for operation, and is suitable for mass detection.

The invention discloses a method for quickly measuring angles of flange holes and aims to realize freeness of site limitation, convenience in operation, accuracy in positioning and high detection accuracy of a method for quickly measuring angles of uniformly distributed holes of parts such as flanges. The technical scheme for implementation includes that an angle scale with measurement scales is made according to a flange radius and a size smaller than a flange hole distance L, a main angle ruler and an auxiliary angle ruler are made on the angle scale and in rotational cross connection aroundthe axis of a rotating shaft center hole O, same sides of the main angle ruler and the auxiliary angle ruler are radially provided with L-shaped notches symmetrical in opposite directions, and an extension connection line of each L-shaped notch and a circle center of a corresponding hole plunger positioning hole in an opposite end are collinear through the center hole O to form a scissors difference rotating pair; two hole plunger bolts identical in shape and size are inserted into to-be-tested flange holes through the hole plunger positioning holes, and a value of an angle between the auxiliary angle ruler and the main angle ruler is read out to obtain an included angle between the two to-be-tested flange holes of a flange face.

The invention belongs to the field of virus detection in the biological technology and particularly relates to a preparation method of a nucleic acid sample for diagnosing a shrimp white spot syndrome virus disease and a method for detecting a shrimp white spot syndrome virus by using the nucleic acid sample subjected to LAMP (Loop-mediated Isothermal Amplification). The method for detecting a shrimp white spot syndrome virus comprises the following steps: taking killed fresh shrimp tissue, slightly shearing the killed fresh shrimp tissue and adding the sheared shrimp tissue into buffer solution III; quickly boiling and centrifuging to obtain supernate as a template for amplifying nucleic acid; after carrying out the LAMP, adding SYBR Green I into the loop-mediated isothermal amplification(LAMP) diluted by thousand folds or ten thousand folds; and judging a detection result by observing whether to exist an excited green fluorescent result through naked eyes under the ultraviolet excitation. The invention has the characteristics of high sensitivity, high specificity, simple and convenient operation, short time consumption, fewer required samples, low cost and the like.

The invention discloses a primer group for identifying avian adenovirus type 4 and a chicken infectious anemia virus. The primer group comprises a primer pair I and a primer pair II; the primer pair Iconsists of a primer FadV-4 F and a primer FadV-4R; the primer pair II consists of a primer CIAV-F and a primer CIAV-R; the primers FadV-4F, FadV-4R, CIAV-F and CIAV-R have base sequences of SEQ. ID. NO. 1 to SEQ. ID. NO. 4 respectively. Accordingly, an inventor further establishes a corresponding detection and identification method and develops a corresponding kit. Established double PCR can beused for rapidly detecting mixed infection of the avian adenovirus type 4 and the chicken infectious anemia virus, is suitable for mass detection, can save the cost and the time, can further reduce pollution and has very high practical value.

The invention discloses a tool for testing the degree of symmetry of a gear key groove, and the tool comprises a central spindle, a measuring column, and a dial indicator. The central spindle is located in a central hole of a gear in a sleeved manner. The periphery of the central spindle is provided with a positioning key which is corresponding to the key groove on the central hole of the gear. One end, close to the gear, of the measuring column is provided with a tooth groove matched with the shape of the gear. The axis of the measuring column, the central line of the tooth groove and the central line of the positioning key are collinear with the center of the central spindle. The measuring column can swing around one end far from the gear. The central spindle can measure the swinging of the measuring column after being equipped with gears with different errors, and the dial indicator is used for measuring the swinging amount so as to calculate the degree of symmetry of the gear key groove. The tool can detect the degree of symmetry of the gear key groove relative to the tooth thickness, is convenient to operate, does not need three-dimensional operation, and is suitable for batch detection.

The invention relates to the field of welding quality detection, in particular to a quality detection device. The quality detection device comprises an integrated probe set, a driving module and a collection module, wherein the integrated probe set comprises multiple integrated probe components arranged in pairs, each integrated probe component is composed of a driving end and a collection end, the driving end of each integrated probe component is matched with the driving end of another integrated probe component arranged in pairs, and the collection end of each integrated probe component is matched with the collection end of the corresponding integrated probe component arranged in pairs. The invention furthermore relates to a quality detection system, a quality detection method and the integrated probe component. According to the designed quality detection device and system and the quality detection method based on a weld joint, through cooperation of the integrated probe set, the driving module and the collection module, rapid detection of the welding quality of the whole weld joint is realized.

The invention discloses a sugarcane streak mosaic virus double-antibody sandwich enzyme-linked immunosorbent assay kit as well as a preparation method and detection method of the kit. The kit containsa micro-hole plate for ELISA, an enzyme labeled antibody, a buffer solution, a developing solution and a stop solution, wherein the micro-hole plate is coated with an SCSMV specific polyclonal antibody, and the enzyme labeled antibody is horseradish peroxidase labeled SCSMV specific polyclonal antibody. An immunogen adopted in the kit is a genetically engineered expressed SCSMV coat protein antigen. The invention further discloses a detection method for sugarcane streak mosaic viruses. A rapid, sensitive, simple, convenient, economic and practical method is provided for the detection of the sugarcane streak mosaic viruses in the serological level, and an effective method is provided for the prevention and treatment of the sugarcane streak mosaic viruses in the production of sugarcane andthe preparation of virus-free seedlings of the sugarcane streak mosaic viruses.

The invention discloses a primer set for identifying chicken parvovirus and bird nephritis virus and application thereof. The primer set comprises a primer pair I and a primer pair II. The primer pair I is composed of a primer ChPV-F and a primer ChPV-R, and the primer pair II is composed of a primer ANV-F and a primer ANV-R; the primers ChPV-F, ChPV-R, ANV-F and ANV-R have base sequences shown in sequence tables SEQ.ID.NO.1-4. Accordingly, the invention further discloses a corresponding detection and identification method and a corresponding kit. The built dual PCR can be used for rapidly detecting mixed infection of chicken parvovirus and bird nephritis virus, and is suitable for large-batch detection, the cost can be saved, the time can be shortened, contaminations can be reduced, and the high practical value is obtained.

The present invention discloses a digital PCR platform-based miRNA detection quality control kit, which comprises a synthesized miRNA, a reverse transcription primer, a detection upper primer, a detection lower primer and DEPC water. The invention also discloses an application method of the kit. The synthesized miRNA provided by the kit absolutely has no similar sequence in a human body, so that the false positive amplification of PCR is avoided. The digital PCR platform-based miRNA detection quality control kit can be used for the quality control of an exogenous internal reference miRNA used for digital PCR detection. Since the miRNA molecule itself is stable in structure and not prone to degradation, the long-term and stable preservation of the miRNA molecule is realized. In addition, by adopting the reverse transcription primer and the detection primers provided by the kit, stable and reliable detection results can be obtained during the detection of a digital PCR platform. During usage, the kit is simple in operation and convenient to use, thus being suitable for mass detection.

The invention discloses a primer group, a kit and a method for identifying salmonella pullorum and salmonella gallinarum. The primer group comprises two pairs of specific primers, wherein the two pairs of specific primers are respectively primer 1 and primer 2 as well as primer 3 and primer 4, wherein the primer 1 is as shown in SEQ ID NO: 1, the primer 2 is as shown in SEQ ID NO: 2, the primer 3 is as shown in SEQ ID NO: 3 and the primer 4 is as shown in SEQ ID NO: 4; the kit contains the primer group; and the primer group is used for detecting to-be-detected bacteria by virtue of double PCR amplification. The primer group for identifying salmonella pullorum and salmonella gallinarum disclosed by the invention is strong in detection reaction specificity; through a PCR reaction, the salmonella pullorum and the salmonella gallinarum can be simultaneously detected from a sample, with high detection reaction sensitivity; and a detection process is rapid and efficient, and suitable for mass detection.

The invention relates to the field of detecting jigs, in particular to a detection jig for back plates of a display screen. The detection jig is characterized in that the detection jig has a hollow structure; the periphery of the detection jig is provided with a plurality of bumps, recesses and solid parts which are matched with the back plates; the detection jig capable of moving upwards and downwards is arranged above a rotatable round table; the bumps are matched with the recesses of the back plates; the recesses are matched with the bumps of the back plates; the solid pats are matched with the holes of the back plates; and a plurality of back plates are arranged on the rotatable round table, and are arranged symmetrically along the circle center of the round table. Due to the adoption of the detection jig, the entire detection process is simple and convenient; and the detection jig is suitable for batch detection of back plates.

The invention relates to the technical field of biological information, and discloses a method for noninvasive identification of pseudo-female Chinese softshell turtle through fluorescent quantitativePCR. The method comprises the following specific steps of setting a pair of primer groups for sexual identification of the pseudo-female Chinese softshell turtle, wherein the primer groups comprise primer pairs designed by DNA sequences of target genes 18S rRNA and Gapdh; performing fluorescent quantitative PCR data processing by adopting a 2-[delta][delta]Ct method, using the Gapdh as a reference gene, and calculating the relative copy number of the 18S rRNA in a genome; and by utilizing the fact that the DNA sequence copy number of the 18S rRNA on the W chromosome of the Chinese softshell turtle is higher than that on the Z chromosome, screening out the pseudo-female Chinese softshell turtle with the ovarian gonad and the ZZ genotype. According to the identification method, the fluorescent quantitative PCR technology is used for sexual identification of the Chinese softshell turtle, detection can be achieved only through a trace amount of blood, non-invasive collection of tail bloodof the Chinese softshell turtle has no influence on health of the Chinese softshell turtle, the method has the characteristics of being efficient, high in sensitivity, good in stability and high in specificity, the blank of pseudo-female Chinese softshell turtle identification is filled, breeding of all male softshell turtle fries is facilitated, and the breeding benefit is improved.

The invention discloses a primer pair for identifying chicken parvovirus and application thereof. The primer pair provided by the invention consists of primers ChPV-F and ChPV-R which are as shown by sequence tables 1 and 2 respectively. The invention also discloses application of the primer pair to the identification whether a to-be-detected virus is the chicken parvovirus or not and application to the identification whether a to-be-detected sample contains the chicken parvovirus or not. A two-temperature PCR (Polymerase Chain Reaction) established by the invention can be used for quickly detecting the chicken parvovirus, and is applicable to large-batch detection; not only can the cost be economized and the time be saved, but also the pollution can be reduced. The primer pair for identifying the chicken parvovirus and the application thereof have quite high practical value.

The invention discloses an intelligent electric energy meter structural member leading-out terminal strength tooling. The intelligent electric energy meter structural member leading-out terminal strength tooling comprises a bottom plate, a detecting meter and a fixing component used for fixing the leading-out terminals to the detecting meter; the detecting meter comprises a meter case and at leastfour sets of detection components on one side of the meter case used for testing the leading-out terminals; both the meter case and the fixing component are connected on the bottom plate, and the fixing component is opposite to the side of the meter case provided with the detecting component. Through setting the spring force of a spring to a required strength of the leading-out terminal, the fixing component fixes the leading-out terminal to the detecting component, and then whether the strength of the leading-out terminal meets the requirements or not can be tested; and the four leading-outterminals of the intelligent electric energy meter structural member can be tested simultaneously by each test. The intelligent electric energy meter structural member leading-out terminal strength tooling has the advantages of simple structure, convenient operation and high detection efficiency, and is suitable for large-scale detection of the intelligent electric energy meter structural members.

The invention discloses a testing fixture of quickly measuring the location degree of bend axle flange end holes. The testing fixture of quickly measuring the location degree of bend axle flange end holes includes a location disk, a thin nut, a handle, a pinhole testing pin and screw hole testing pins, wherein one end surface of the location disk is provided with a ring-shaped location groove; a slantingly-downward escape is arranged along one circle of the outer side edge of the ring-shaped location groove on the location disk; mounting holes 1 are formed in the ring-shaped location groove along one circumferential circle of the ring-shaped location groove; a mounting hole 2 is formed between two of the mounting holes 1; each mounting hole 1 is in interference fit with the screw hole testing pin; the mounting hole 2 is in interference fit with the pinhole testing pin; a screw hole is formed in the middle location of the other end surface of the location disk; the screw hole is provided with the handle; a ring-shaped transition groove and a location hole are successively formed on the location disk from the ring-shaped location groove to the screw hole; and the upper port end of the location hole is provided with a chamfer. Therefore, the testing fixture of quickly measuring the location degree of bend axle flange end holes has the advantages of being convenient for arrangement of manpower resource, greatly reducing the labor intensity of workers, greatly improving the detection efficiency and being suitable for mass detection.

The invention discloses a method for quickly detecting ploidy of new brassica hexaploid germplasm. The method includes: (1) taking a third leaf of a new brassica germplasm from an interior leaf to the outside, putting in a steel ball containing centrifugal tube, adding extraction buffer liquor into the centrifugal tube, standing for 5-20min at a temperature ranging from -20 to -10 DEG C, and grinding in a grinder; (2) filtering the ground leaf through a filter screen to obtain filtrate, adding RNAse solution into the filtrate, and incubating for 20-30min at 37 DEG C; (3) adding staining fluid into the incubated filtrate, and performing lightproof staining to obtain a unicellular suspension sample; (4) using a flow cytometer for detecting the unicellular suspension sample. By adoption of the grinder for grinding leaf tissues, a great quantity of unicells are generated in the unicellular suspension sample, and broken cells are few. In addition, the method is high in detection efficiency and suitable for large-scale detection.

The present invention discloses a primer set for identifying chicken parvovirus and chicken infectious anemia virus. The primer set comprises a primer pair I and a primer pair II. The primer pair I iscomposed of primers ChPV-F and ChPV-R, and the primer pair II is composed of primers CIAV-F and CIAV-R; and the primers ChPV-F, ChPV-R, CIAV-F, and CIAV-R separately have base sequences of SEQ.ID.NO.1 to SEQ.ID.NO.4 in the sequence table . On the basis, inventors also establish corresponding detection and identification methods and develop corresponding kits. An established duplex PCR can be usedfor rapid detection of mixed infection of the chicken parvovirus and chicken infectious anemia virus, is suitable for large-scale detection, can save costs and time, also reduces pollution and has very high practical value.

The invention discloses an automatic resistance value sorting device and sorting process. The device comprises a sorting rotating disc; and a first positioning air cylinder, a second positioning air cylinder and a third positioning air cylinder are fixed on air cylinder fixing bases, the first positioning air cylinder is connected with the first air cylinder fixing base through an action end, thesecond positioning air cylinder is connected with the second air cylinder fixing base through an action end, the third positioning air cylinder is connected with the third air cylinder fixing base through an action end, the first air cylinder fixing base is connected with a first negative pressure feeding air cylinder through a screw, the first negative pressure feeding air cylinder is connected with a negative pressure suction cup through an action end, and a fourth negative pressure feeding air cylinder is connected with the negative pressure suction cup through an action end. The device andthe process has the characteristics that vibration hopper automatic layout design is adopted, the layout automation is realized, the resistance values can be automatically sorted, the operation is simple, the resistance values are accurate, the percent of pass is high, the efficiency is high, the equipment is suitable for mass detection, the defect of manual sorting is overcome, the manual work is replaced by machines, and the labor cost is reduced.