1 results about "Deoxyribonucleoside triphosphate" patented technology

The application belongs to the technical field of sequencing-by-synthesis chip, and particularly relates to a three-dimensional porous array enhanced SBS sequencing chip and a preparation method thereof, which comprises a silicon wafer as a substrate and a light-controlled reversible terminator reaction system; the silicon wafer surface has a mesoporous film, and the mesoporous film has reaction cavities and a closed microchannel network; the inner wall of the mesoporous film is grafted with mercaptopropionic acid and covalently coupled with an amino-modified probe; the reaction cavity bottom is etched with a cylindrical array; the light-controlled reversible terminator reaction system comprises deoxyribonucleotide triphosphates, the 3'-OH end of the deoxyribonucleotide triphosphates is introduced with a protective group, and the 5' end amino group of the deoxyribonucleotide triphosphates is specifically combined with a fluorescent group; and the application effectively breaks through the technical bottlenecks of the existing NGS sequencing chip in terms of throughput, accuracy, cost and adaptability of complex samples.