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102results about How to "Intuitive and reliable results" patented technology

Milk allergen test plate and preparation method thereof

The invention discloses a milk allergen test plate, which belongs to the gold immunochromatography detection. According to the milk allergen test plate disclosed by the invention, two ends of a PVC (polrvinyl chloride) base plate are respectively provided with a to-be-tested sample zone and an absorption zone. Colloidal gold mark antigens prepared by respectively marking colloidal gold into casein, beta lactoglobulin and alpha lactalbumin and mixing are orderly arranged between the sample zone to be tested and the absorption zone, and a nitrocellulose membrane is respectively provided with a detection zone coated with mixed milk allergen and a quality control zone coated with anti-beta lactoglobulin antibodies. In detection, color lines are formed in the detection zone and the quality contol zone when an immune complex is formed by specific milk antibodies contained in samples. If the samples do not contain specific milk antibodies, the detection zone does not display color, and only one color line is formed in the quality control zone. The milk allergen test plate disclosed by the invention with the design has the advantages of strong pertinence to the allergen detection, simplicity in operation, low cost and high sensitivity and the like, and can prevent the phenomenon of missed diagnosis in the single antibody detection. The milk allergen test plate is applied for rapidly screening patient allergic to milk, and is especially suitable for being used by primary medical treatment units.
Owner:江苏迈源生物科技有限公司

Method for identifying baijiu of different brands by combining three-dimensional fluorescence spectrum and parallel factor

The invention discloses a method for identifying baijiu of different brands by combining a three-dimensional fluorescence spectrum and a parallel factor, belonging to the technical field of baijiu identification. The method disclosed by the invention comprises the following steps: (1) obtaining the three-dimensional fluorescence spectrum data of baijiu of different brands by using a fluorescence spectrophotometer; (2) establishing an identification model of baijiu of different brands, namely decomposing the spectrum data by utilizing a parallel factor method, thus obtaining a loading matrix and a concentration score, and by taking the concentration score as input of a support vector machine, establishing an identification model of the brands of the baijiu; and (3) predicting the brands of the baijiu, specifically, for the predicted sample, performing testing to obtain the three-dimensional fluorescence spectrum data, and obtaining the concentration score by utilizing a genetic algorithm and by keeping the loading matrix invariable, inputting the concentration score into the identification model and outputting the predicted brand value. According to the method disclosed by the invention, a complicated chemical separation process is avoided, the method has the advantages of rapidness, safety, simplicity in operation, low price, nondestructive property and the like, and the identification model of baiju in different places of origin can be further established.
Owner:JIANGNAN UNIV

Openable advanced water pressure and water flow detection device for tunnel

A self-sealing advanced water pressure and water flow detection device for a tunnel comprises an openable plugging device, a telescopic water guiding device and a water pressure and water flow measuring device which are connected sequentially. Before detection, a horizontal advanced drilling machine is used for drilling a hole to the position required to be detected, and a soft stratum adopts casing pipes for wall protection. The device extends into the detection hole, a front-end casing pipe abuts against the hole bottom, a central casing pipe is pushed inwards continuously, and the front-end casing pipe and a first-section casing pipe move relative to each other, so that a steel bracket drives a rubber piece to be stretched, and a closed space is formed. A buckle on the first-section casing pipe is buckled in a groove of the front-end casing pipe, and a sealing device is in the working state. After pipe sections are full of water, a valve at the back end is closed, and water pressure is measured by the aid of a water pressure gauge; the valve is opened, and the water flow is measured by the aid of a water flow machine; and pore water pressure of a front-end stratum is measured by the aid of a most-front-end pore water pressure sensor. After measurement is completed, the central casing pipe is pushed inwards continuously until the buckle is released from the groove, the steel bracket can be loosened after the central casing pipe is rotated, and the whole device is released.
Owner:CHINA CONSTR SOUTH INVESTMENT CO LTD +2

Method for rapidly analyzing key aroma compound of yellow rice wine

The invention relates to a method for rapidly analyzing a key aroma compound of yellow rice wine. The method comprises the steps of: (1), extracting an aroma compound in yellow rice wine; (2), performing qualitative and quantitative analysis on the aroma compound in the yellow rice wine by using a gas chromatography and mass spectrometry combination technology; (3), by utilizing a sensory evaluation method, performing descriptive analysis on the yellow rice wine by selecting eight sensory attributes; (4), inspecting the correlation relations between the aroma compound and the eight sensory attributes by utilizing a partial least square method, and determining a characteristic aroma component in the yellow rice wine according to the correlation between the aroma component and the sensory attribute; (5), analyzing an aroma active compound in the yellow rice wine by utilizing a gas chromatography and olfactory combination technology; (6), recombining the aroma of the yellow rice wine by utilizing an aroma model established by the key aroma compound; and (7), further verifying and determining the key aroma compound of the yellow rice wine by utilizing an omission experiment. The methodin the invention is simple to operate and intuitional and reliable in result, and can be used for identifying aroma compounds in different kinds of yellow rice wine; and thus, the applicability is wide.
Owner:SHANGHAI INST OF TECH

SSR molecular marker of fruit mulberry variety 'Yueshen 123', core primer group, kit and application thereof

The invention discloses an SSR molecular marker of a fruit mulberry variety 'Yueshen 123', a core primer group, a kit and application thereof. According to the SSR molecular marker, the core primer group, the kit and the application thereof, a fluorescent SSR technology is utilized, 300 pairs of SSR primers designed and developed by a mulberry genome sequence are screened, which confirms that core primers marked with M10360 and M29602 can amplify two specific bands for the new fruit mulberry variety 'Yueshen 123', and the new fruit mulberry variety 'Yueshen 123' can be identified from fruit mulberry strains and can be used for rapid identification and detection of the new fruit mulberry variety 'Yueshen 123'. The 'Yueshen 123' is identified by directly detecting the M10360 marker and/or the M29602 marker of the 'Yueshen 123', the uncertainty of external morphological characteristics on identification of the 'Yueshen 123' is overcome, the operation is simple, the detection efficiency is high, the result is reliable and visual, a scientific technical guarantee is provided for protection of the new fruit mulberry variety 'Yueshen 123', and the SSR molecular marker is beneficial to popularization, utilization and protection of the variety.
Owner:SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI

SSR (Simple sequence repeat) molecular marker of fruit mulberry variety 'Yue mulberry 74', core primer group of SSR molecular marker, kit and application

The invention discloses an SSR (simple sequence repeat) molecular marker of a fruit mulberry variety 'Yue mulberry 74', a core primer group of the SSR molecular marker, a kit and an application. A fluorescent SSR technology is utilized, 200 pairs of SSR primers developed through mulberry genome sequence design are screened, it is confirmed that M792 and M20197 marked core primers can amplify two specific bands for the new fruit mulberry variety 'Yue mulberry74' respectively, and the new fruit mulberry variety 'Yuemulberry 74' can be identified from fruit mulberry strains respectively. The molecular marker can be used for rapid identification and detection of the new fruit mulberry variety 'Yue mulberry 74'. The M792 marker and / or the M20197 marker of 'Yue mulberry 74' are / is directly detected to identify 'Yue mulberry 74', the uncertainty of external morphological characteristics on identification of 'Yue mulberry 74' is overcome, the operation is simple, the detection efficiency is high, the result is reliable and visual, a scientific technical guarantee is provided for protection of the new fruit mulberry variety 'Yue mulberry 74', and popularization, utilization and protection of the variety are facilitated.
Owner:SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI

Method for quantitatively determining sympathatic detonation of explosive

The invention provides a method for quantitatively determining sympathatic detonation of an explosive, and belongs to the technical field of blasting. The method comprises the following steps: fixinga continuous resistance wire probe to centers of a detonating cartridge and a detonated cartridge, integrally placing the detonating cartridge and the detonated cartridge with the continuous resistance wire probe in a semicircular pipe groove of a test support, and keeping the cartridges in the same axis; regulating the sympathetic detonation distance to be in preset value L by moving the detonated cartridge, and connecting a probe led out from the tail end of the detonated cartridge to a signal acquisition instrument through a coaxial cable; inserting a detonator into a detonating end of thedetonating cartridge, starting the signal acquisition instrument, detonating the explosive, and recording a voltage signal curve of the continuous resistance wire probe by the signal acquisition instrument; converting, thus obtaining a detonation wave-impact wave time travel curve; comparing a detonation wave travel with the length of the cartridges, thus judging whether the detonated cartridge isin sympathatic detonation or not. A measurement system is simple, the cost is lower, a result is direct and reliable and is basically not affected by a testing environment, and the explosive amount is not limited.
Owner:DALIAN UNIV OF TECH

Hopper scale on-line automatic calibration method based on small number of weights

The invention relates to a hopper on-line automatic calibration method based on a small number of weights. The method comprises the steps that 1 the repeatability is tested; 2 the number of times of weight loading and the number of calibration weights are calculated according to the repeatability test result; 3 the number of times of weight loading and the number of calibration weights are set into an online automatic calibration weighing program; 4 according to the number of calibration weights, weights and a lift electric screw push rod thereof are arranged around a hopper scale; 5 a hopper scale on-line automatic calibration system based on a small number of weights is formed; 6 the blanking fixed value automatic control system of a weighed object is converted into an online automatic calibration mode; and 7 if a calibration result is normal, automatically restoring to the fixed value automatic blanking mode is carried out, otherwise repair is prompted to notify a relevant position to change a spare bin. Automatic fast calibration of all hopper scales can be timely realized at the same time. Full range reliable calibration of a small amount of weights is realized. Calibration and use processes are simultaneous and automatic. A calibration process is free of manual participation. The calibration cost is low.
Owner:SD STEEL RIZHAO CO LTD

SSR (Simple Sequence Repeat) molecular marker of new fruit mulberry variety 'Yueshen 201', core primer group of SSR molecular marker, kit and application of SSR molecular marker, or core primer group or kit

The invention discloses an SSR (Simple Sequence Repeat) molecular marker of a new fruit mulberry variety 'Yueshen 201', a core primer group of the SSR molecular marker, a kit and an application of the SSR molecular marker, or the core primer group or the kit. A fluorescent SSR technology is utilized, 300 pairs of SSR primers developed through mulberry genome sequence design are screened, it is confirmed that core primers marked with M20310 and M252 can amplify two specific bands for the new fruit mulberry variety 'Yueshen 201' respectively, the new fruit mulberry variety 'Yueshen 201' can be identified from fruit mulberry strains respectively, and the new fruit mulberry variety 'Yueshen 201'can be identified from the fruit mulberry strains respectively. The molecular marker can be used for rapid identification and detection of the new fruit mulberry variety 'Yueshen 201'. According to the invention, the M20310 marker and/or the M252 marker of the 'Yueshen 201' are/is directly detected to identify the 'Yueshen 201', the uncertainty of external morphological characteristics on identification of the 'Yueshen 201' is overcome, the operation is simple, the detection efficiency is high, the result is reliable and visual, a scientific technical guarantee is provided for protection of the new fruit mulberry variety 'Yueshen 201', and the molecular marker, the core primer group and the kit are beneficial to popularization, utilization and protection of the varieties.
Owner:SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI

Method for detecting tumor mutant gene in blood

The invention relates to a method for detecting a tumor mutant gene in blood. The method comprises the following steps of: (1) preparing a polyethylene oxide sieving medium containing TBE (Tetrabromoethane); (2) extracting a genome DNA (Deoxyribonucleic Acid) in a blood sample; (3) amplifying a gene target fragment needing to be detected: performing conventional PCR (Polymerase Chain Reaction) amplification by taking the genome DNA as an amplifying template to obtain a p53 gene PCR amplification sample and a k-ras gene PCR amplification sample; (4) treating the samples: performing denaturation or enzyme digestion on the p53 gene PCR amplification sample and the k-ras gene PCR amplification sample to obtain a denatured sample solution and an enzyme digestion solution respectively; and (5) detecting the samples: adding a 10*SYBR Green I fluorescence dye and the genome DNA into the denatured sample solution and the enzyme digestion solution respectively, uniformly mixing, adding deionized water till a system is 30-50 mu l, automatically filling the polyethylene oxide sieving medium by using a capillary electrophoresis apparatus pressure system and performing CE-SSCP (Capillary Electrophoresis-Single Strand Conformation Polymorphism) detection or CE-RFLP (Capillary Electrophoresis-Restriction Fragment Length Polymorphism) detection respectively. The method is easy to operate, and has high repeatability.
Owner:王荣

Method and device for dynamic non-destructive measurement of CRTSII type plate-type ballastless track seam

The invention discloses a method and a device for dynamic non-destructive measurement of CRTSII type plate-type ballastless track seam. The method comprises the following steps: firstly enabling an ultrasonic pulse transmitting receiver to generate a sine wave exciting signal modulated by a Hanning window, amplifying the exciting signal by a preamplifier to drive an air-coupled ultrasonic transmitting transducer to convert the electrical signal into an ultrasonic wave to hit a CRTSII type plate-type ballastless track plate, then adopting an air-coupled ultrasonic receiving transducer to collect a guided wave echo signal, amplifying the guided wave echo signal by a post-amplifier, subjecting to A/D conversion, transmitting to the ultrasonic pulse transmitting receiver, then transmitting thereceived guided wave echo digital signal to a computer by the ultrasonic pulse transmitting receiver, performing signal processing and analysis by the computer adopting MATLAB software, and judging whether a seam defect exists by comparing an obtained first wave amplitude with a preset threshold value. By adopting the method and the device provided by the invention, the high-efficiency, non-destructive and real-time measurement of the seam defect of a CRTSII type plate-type ballastless track can be realized.
Owner:SHANGHAI UNIV OF ENG SCI

Molecular marker for identifying fruit mulberry variety 'Guangdong mulberry 143', identification primer group, kit and application

The invention discloses a molecular marker for identifying a fruit mulberry variety 'Guangdong mulberry 143', an identification primer group, a kit and application. The fluorescent SSR technology is utilized, 300 pairs of SSR primers designed and developed for the mulberry genome sequence are screened, it is confirmed that the core primers marked with M20541 and M22794 can amplify three specific bands for the new fruit mulberry variety 'Yuegui 143' respectively, the new fruit mulberry variety 'Yuegui 143' can be identified from the fruit mulberry strains respectively, and the specific bands of the new fruit mulberry variety 'Yuegui 143' and the specific bands of the new fruit mulberry variety 'Yuegui 143' can be identified from the fruit mulberry strains respectively. The method can be used for rapid identification and detection of a new fruit mulberry variety 'Guangdong mulberry 143'. According to the method, the M20541 marker and / or the M22794 marker of 'Yueyan mulberry 143' are / is directly detected to identify the 'Yueyan mulberry 143', the uncertainty of external morphological characteristics on identification of the 'Yueyan mulberry 143' is overcome, the operation is simple, the detection efficiency is high, the result is reliable and visual, a scientific technical guarantee is provided for protection of the new fruit mulberry 'Yueyan mulberry 143', and the method is beneficial to popularization, utilization and protection of the 'Yueyan mulberry 143'.
Owner:SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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