102results about How to "Intuitive and reliable results" patented technology

The invention discloses a milk allergen test plate, which belongs to the gold immunochromatography detection. According to the milk allergen test plate disclosed by the invention, two ends of a PVC (polrvinyl chloride) base plate are respectively provided with a to-be-tested sample zone and an absorption zone. Colloidal gold mark antigens prepared by respectively marking colloidal gold into casein, beta lactoglobulin and alpha lactalbumin and mixing are orderly arranged between the sample zone to be tested and the absorption zone, and a nitrocellulose membrane is respectively provided with a detection zone coated with mixed milk allergen and a quality control zone coated with anti-beta lactoglobulin antibodies. In detection, color lines are formed in the detection zone and the quality contol zone when an immune complex is formed by specific milk antibodies contained in samples. If the samples do not contain specific milk antibodies, the detection zone does not display color, and only one color line is formed in the quality control zone. The milk allergen test plate disclosed by the invention with the design has the advantages of strong pertinence to the allergen detection, simplicity in operation, low cost and high sensitivity and the like, and can prevent the phenomenon of missed diagnosis in the single antibody detection. The milk allergen test plate is applied for rapidly screening patient allergic to milk, and is especially suitable for being used by primary medical treatment units.

The invention discloses a server network behavior description method. The method comprises the steps that (1) traffic information in and out of a server is acquired; (2) according to traffic attributes, the traffic information is extracted, and according to a time window, traffic corresponding to each traffic attribute is counted to form historical data; (3) the historical data are calculated to acquire system parameters based on the traffic structure stability; (4) a dynamic normal traffic contour is built; (5) the current traffic structure is constructed; and (6) a difference measurement method is used to compare the normal traffic contour and the current traffic structure, and whether a network is normal is judged according to the size of a difference value. According to the invention, the server network behavior description method can adapt to an increasingly complex network environment, can detect a part of new network attacks, and can take initiative in detection.

The invention discloses a method for identifying strong flavor Baijiu by combining a three-dimensional fluorescence spectrum with PCA-SVM and belongs to the technical field of Baijiu identification. The method comprises the following steps: measuring the three-dimensional fluorescence spectrum of strong flavor Baijiu of different brands by using a fluorescence spectrophotometer, so as to acquire data of the three-dimensional fluorescence spectrum; and solving second-order deflection of fluorescence intensity on the emission wavelength by virtue of the data of the spectrum, compressing the data by using wavelet transform, performing principal component analysis on the acquired novel data after preprocessing, combining the principal component analysis with a support vector machine, searching the optimal parameters of the support vector machine by using a cross validation method, and establishing a prediction model under the optimal parameters so as to achieve classification and identification of the strong flavor Baijiu of the different brands. The method disclosed by the invention provides help for anti-counterfeiting of Baijiu in the market.

The invention discloses a phase-change temperature testing system, which has the following structure that: a light-through hole is reserved on a cover plate of a heating furnace in which a furnace chamber is arranged; a sample holder is positioned in the furnace chamber and is positioned under the light-through hole; a laser and a photoelectric detector are arranged above the light-through hole; and the photoelectric detector is positioned on a light path of a sample to be tested for reflecting laser beams. The phase-change temperature testing system can be additionally provided with a signal amplification and acquisition circuit, a temperature control sensing circuit and a data processor. The system has relatively higher testing sensitivity, can test the phase-change temperature of a thin film thinner than 1 nm, directly test the phase-change temperature of a thin film sample without damage to the sample and obtain more material thermodynamic parameters through temperature-change measurement at different heating rates, and has the characteristics of simple operation, low cost and relatively higher testing reliability.

The invention discloses a method for identifying baijiu of different brands by combining a three-dimensional fluorescence spectrum and a parallel factor, belonging to the technical field of baijiu identification. The method disclosed by the invention comprises the following steps: (1) obtaining the three-dimensional fluorescence spectrum data of baijiu of different brands by using a fluorescence spectrophotometer; (2) establishing an identification model of baijiu of different brands, namely decomposing the spectrum data by utilizing a parallel factor method, thus obtaining a loading matrix and a concentration score, and by taking the concentration score as input of a support vector machine, establishing an identification model of the brands of the baijiu; and (3) predicting the brands of the baijiu, specifically, for the predicted sample, performing testing to obtain the three-dimensional fluorescence spectrum data, and obtaining the concentration score by utilizing a genetic algorithm and by keeping the loading matrix invariable, inputting the concentration score into the identification model and outputting the predicted brand value. According to the method disclosed by the invention, a complicated chemical separation process is avoided, the method has the advantages of rapidness, safety, simplicity in operation, low price, nondestructive property and the like, and the identification model of baiju in different places of origin can be further established.

The invention discloses a structure for detecting a post-grouted inserting type sleeve and steel bar connector through an endoscope method and a method applying the structure and belongs to the technical field of detection. Whether the position of a steel bar in a sleeve is in place or not is observed through an industrial endoscope. According to the method, operation is easy and convenient, the result is visual and reliable, the applicable working condition is wide, and the influence of the site construction condition on themethod is less; and meanwhile, whether a grouting channel and an overflowing channel are unobstructed or not can be inspected, and the guarantee is provided for the quality of subsequent grouting construction.

A self-sealing advanced water pressure and water flow detection device for a tunnel comprises an openable plugging device, a telescopic water guiding device and a water pressure and water flow measuring device which are connected sequentially. Before detection, a horizontal advanced drilling machine is used for drilling a hole to the position required to be detected, and a soft stratum adopts casing pipes for wall protection. The device extends into the detection hole, a front-end casing pipe abuts against the hole bottom, a central casing pipe is pushed inwards continuously, and the front-end casing pipe and a first-section casing pipe move relative to each other, so that a steel bracket drives a rubber piece to be stretched, and a closed space is formed. A buckle on the first-section casing pipe is buckled in a groove of the front-end casing pipe, and a sealing device is in the working state. After pipe sections are full of water, a valve at the back end is closed, and water pressure is measured by the aid of a water pressure gauge; the valve is opened, and the water flow is measured by the aid of a water flow machine; and pore water pressure of a front-end stratum is measured by the aid of a most-front-end pore water pressure sensor. After measurement is completed, the central casing pipe is pushed inwards continuously until the buckle is released from the groove, the steel bracket can be loosened after the central casing pipe is rotated, and the whole device is released.

The invention provides a method for classifying railway track stations based on characteristics of a public transportation connection radiating area. The method comprises the steps of collecting cardreading data which forms a transfer behavior, analyzing the characteristics of the public transportation connection radiating area according to an identified transfer behavior, counting characteristicparameters of the public transportation connection radiating area, carrying out normalization and standardization on the selected characteristic parameters, classifying the railway track stations byusing a k-means clustering method, and finally quantitatively evaluating the classified stations by using a fuzzy element theory. According to the method, from an actual angle and a transfer need angle, the railway track stations are reasonably divided, and a certain reference value is provided for rail transit planning and operation optimization.

The invention discloses an underground metal mine goaf hazard degree grading evaluation method, which comprises the following steps: firstly, selecting 16 indexes to construct an underground metal mine goaf hazard degree grading evaluation index system, dividing hazard degrees into four grades, and determining a grading standard; then, determining the comprehensive weight of each index by adoptingan AHP, entropy weight method and game theory combined weighting method; establishing a matter element extension model for goaf risk degree evaluation; substituting the weight value of each evaluation index into the matter element extension model to obtain each index correlation degree and a comprehensive correlation degree value which belong to each risk degree grade; and finally, determining the danger degree level according to the maximum correlation degree principle. According to the method, the goaf hazard level can be evaluated comprehensively, objectively and accurately, the evaluationprocess is clear, the evaluation result is objective, and a new method can be provided for grading evaluation of the underground metal mine goaf hazard level.

The invention relates to a method for rapidly analyzing a key aroma compound of yellow rice wine. The method comprises the steps of: (1), extracting an aroma compound in yellow rice wine; (2), performing qualitative and quantitative analysis on the aroma compound in the yellow rice wine by using a gas chromatography and mass spectrometry combination technology; (3), by utilizing a sensory evaluation method, performing descriptive analysis on the yellow rice wine by selecting eight sensory attributes; (4), inspecting the correlation relations between the aroma compound and the eight sensory attributes by utilizing a partial least square method, and determining a characteristic aroma component in the yellow rice wine according to the correlation between the aroma component and the sensory attribute; (5), analyzing an aroma active compound in the yellow rice wine by utilizing a gas chromatography and olfactory combination technology; (6), recombining the aroma of the yellow rice wine by utilizing an aroma model established by the key aroma compound; and (7), further verifying and determining the key aroma compound of the yellow rice wine by utilizing an omission experiment. The methodin the invention is simple to operate and intuitional and reliable in result, and can be used for identifying aroma compounds in different kinds of yellow rice wine; and thus, the applicability is wide.

The present invention relates to a preparation method of a triple inactivated vaccine for the prevention of H9 subtype newcastle disease, infectious bronchitis and avian influenza, which is characterized in that: the content of the using NDVLaSota stain virus liquid is 10 to 22 percent, the content of IBVM41 stain virus liquid is also 10 to 22 percent and the content of the AIVHL stain virus liquid is 8 to 15 percent; the present invention uses the concentration and inactivation methods; the white oil adjuvant and the immune auxiliaries of Tween, Span and aluminum stearate are added to prepare the triple inactivated vaccine; the present invention realizes the multiple vaccine prevention and achieves the purpose of prevention a plurality of diseases by one needle, which can be used for greatly developing the husbandry, so as to benefit the mankind and improve the living standard of the people.

The invention provides a rail station classification system. Card swiping data which forms a transfer behavior is collected, characteristics of a bus transferring radiation area are analyzed accordingto an identified transfer behavior, characteristic parameters of the bus transferring radiation area are arranged, normalized and standardized, a k-means clustering method is used to classify the rail stations, and a fuzzy metatheory is used to evaluate the classified stations quantitatively. The method is used to classify the rail stations reasonably from the aspect of practical transfer demands, and provides certain reference values for planning and operation optimization of rail transit.

The invention discloses an SSR molecular marker of a fruit mulberry variety 'Yueshen 123', a core primer group, a kit and application thereof. According to the SSR molecular marker, the core primer group, the kit and the application thereof, a fluorescent SSR technology is utilized, 300 pairs of SSR primers designed and developed by a mulberry genome sequence are screened, which confirms that core primers marked with M10360 and M29602 can amplify two specific bands for the new fruit mulberry variety 'Yueshen 123', and the new fruit mulberry variety 'Yueshen 123' can be identified from fruit mulberry strains and can be used for rapid identification and detection of the new fruit mulberry variety 'Yueshen 123'. The 'Yueshen 123' is identified by directly detecting the M10360 marker and / or the M29602 marker of the 'Yueshen 123', the uncertainty of external morphological characteristics on identification of the 'Yueshen 123' is overcome, the operation is simple, the detection efficiency is high, the result is reliable and visual, a scientific technical guarantee is provided for protection of the new fruit mulberry variety 'Yueshen 123', and the SSR molecular marker is beneficial to popularization, utilization and protection of the variety.

The invention discloses an SSR (simple sequence repeat) molecular marker of a fruit mulberry variety 'Yue mulberry 74', a core primer group of the SSR molecular marker, a kit and an application. A fluorescent SSR technology is utilized, 200 pairs of SSR primers developed through mulberry genome sequence design are screened, it is confirmed that M792 and M20197 marked core primers can amplify two specific bands for the new fruit mulberry variety 'Yue mulberry74' respectively, and the new fruit mulberry variety 'Yuemulberry 74' can be identified from fruit mulberry strains respectively. The molecular marker can be used for rapid identification and detection of the new fruit mulberry variety 'Yue mulberry 74'. The M792 marker and / or the M20197 marker of 'Yue mulberry 74' are / is directly detected to identify 'Yue mulberry 74', the uncertainty of external morphological characteristics on identification of 'Yue mulberry 74' is overcome, the operation is simple, the detection efficiency is high, the result is reliable and visual, a scientific technical guarantee is provided for protection of the new fruit mulberry variety 'Yue mulberry 74', and popularization, utilization and protection of the variety are facilitated.

The invention discloses a method for analyzing apple juice esters aroma synergism based on a Sigma-Tau strength method. The method comprises the following steps: performing aroma strength analysis onthe esters A and B and the AB binary mixture, wherein the aroma strength of the esters A is IA, the aroma strength of the esters B is IB, and the aroma strength of the AB binary mixture is IAB; TauA and TauB represent the ratio of the aroma strength value of esters A or B to the sum of the aroma strength value of the esters A and the esters B, namely the TauA=IA / (IA+IB), TauB=IB(IA+IB); Sigma represents the ratio of the aroma strength value of the AB binary mixture to the sum of the sum of the aroma strength value of the esters A and the esters B, namely, the Sigma=IAB(IA+IB). The data obtained by above step is applied to the Sigma-Tau strength relation model by using the Sigma Plot 12.0 software, the aroma strength value change before and after the combination of different esters is analyzed, thereby analyzing the aroma synergism influence of the esters.

The invention provides a method for quantitatively determining sympathatic detonation of an explosive, and belongs to the technical field of blasting. The method comprises the following steps: fixinga continuous resistance wire probe to centers of a detonating cartridge and a detonated cartridge, integrally placing the detonating cartridge and the detonated cartridge with the continuous resistance wire probe in a semicircular pipe groove of a test support, and keeping the cartridges in the same axis; regulating the sympathetic detonation distance to be in preset value L by moving the detonated cartridge, and connecting a probe led out from the tail end of the detonated cartridge to a signal acquisition instrument through a coaxial cable; inserting a detonator into a detonating end of thedetonating cartridge, starting the signal acquisition instrument, detonating the explosive, and recording a voltage signal curve of the continuous resistance wire probe by the signal acquisition instrument; converting, thus obtaining a detonation wave-impact wave time travel curve; comparing a detonation wave travel with the length of the cartridges, thus judging whether the detonated cartridge isin sympathatic detonation or not. A measurement system is simple, the cost is lower, a result is direct and reliable and is basically not affected by a testing environment, and the explosive amount is not limited.

The present invention provides a checking system of special fragment of fluorescence labeling Cannabis sativa L. sexuality gene, wherein, the checking system is a PCR reacting system which uses the fluorescence labeled DM016 as male primer and uses the fluorescence labeled DM029 as female primer and uses the DNA of Cannabis sativa L. as a formwork. The invention simultaneously provides a checking method of the checking system.

A method for reducing content of ethyl carbamate in wine by utilizing secondary distillation belongs to the field of safety control of wine quality. The method comprises the steps of (1) dropping alcoholic strength of raw wine; (2) performing pot type distillation; and (3) protecting distilled wine against high-temperature storage. A pot type distiller is used for carrying out secondary distillation on the raw wine in which water is added to drop alcoholic strength thereof, distilling parameters are controlled strictly, and a suitable way of nipping off unwanted parts is adopted for distillation to obtain secondarily distilled wine with relatively low content of ethyl carbamate EC. The method is a new wine quality safety index control technology, is simple to operate, can efficiently reduce content of ethyl carbamate in the raw wine, accordingly wine quality safety is controlled effectively.

The invention discloses a method for identifying years of Luzhou-flavor liquor through three-dimensional fluorescence spectrum combined with non-negative matrix factorization and belongs to the technical field of liquor identification. The method comprises the steps that firstly, a fluorescence spectrophotometer is used for measuring three-dimensional fluorescence spectrum charts of the Luzhou-flavor liquor of different years, and obtaining three-dimensional fluorescence spectrum data; then adopting a multiplicative non-negative matrix factorization method for decomposing a spectrum data matrix, and treating an obtained coefficient matrix as characteristic parameters of a spectrum matrix; then treating the characteristic parameters as input of a support vector machine, using a cross validation method for searching for the optimum parameter of the support vector machine, establishing a prediction model under the optimum parameter, and achieving classified identification on the Luzhou-flavor liquor of different years. By means of the method, support is provided for identification and development technologies of aged liquor.

The invention relates to a hopper on-line automatic calibration method based on a small number of weights. The method comprises the steps that 1 the repeatability is tested; 2 the number of times of weight loading and the number of calibration weights are calculated according to the repeatability test result; 3 the number of times of weight loading and the number of calibration weights are set into an online automatic calibration weighing program; 4 according to the number of calibration weights, weights and a lift electric screw push rod thereof are arranged around a hopper scale; 5 a hopper scale on-line automatic calibration system based on a small number of weights is formed; 6 the blanking fixed value automatic control system of a weighed object is converted into an online automatic calibration mode; and 7 if a calibration result is normal, automatically restoring to the fixed value automatic blanking mode is carried out, otherwise repair is prompted to notify a relevant position to change a spare bin. Automatic fast calibration of all hopper scales can be timely realized at the same time. Full range reliable calibration of a small amount of weights is realized. Calibration and use processes are simultaneous and automatic. A calibration process is free of manual participation. The calibration cost is low.

The invention discloses an SSR (Simple Sequence Repeat) molecular marker of a new fruit mulberry variety 'Yueshen 201', a core primer group of the SSR molecular marker, a kit and an application of the SSR molecular marker, or the core primer group or the kit. A fluorescent SSR technology is utilized, 300 pairs of SSR primers developed through mulberry genome sequence design are screened, it is confirmed that core primers marked with M20310 and M252 can amplify two specific bands for the new fruit mulberry variety 'Yueshen 201' respectively, the new fruit mulberry variety 'Yueshen 201' can be identified from fruit mulberry strains respectively, and the new fruit mulberry variety 'Yueshen 201'can be identified from the fruit mulberry strains respectively. The molecular marker can be used for rapid identification and detection of the new fruit mulberry variety 'Yueshen 201'. According to the invention, the M20310 marker and / or the M252 marker of the 'Yueshen 201' are / is directly detected to identify the 'Yueshen 201', the uncertainty of external morphological characteristics on identification of the 'Yueshen 201' is overcome, the operation is simple, the detection efficiency is high, the result is reliable and visual, a scientific technical guarantee is provided for protection of the new fruit mulberry variety 'Yueshen 201', and the molecular marker, the core primer group and the kit are beneficial to popularization, utilization and protection of the varieties.

The invention relates to a sandy soil saturation measuring method. The method comprises following steps: (1) adding a sandy soil sample with a volume of V into a sample container, and vacuumizing thesandy soil sample and a constant head device in the sample container; (2) introducing carbon dioxide into the vacuumized sandy soil sample and the constant head device; (3) repeating steps (1) and (2)for many times; (4) simultaneously vacuumizing the sandy soil sample and the constant head device; (5) carrying out constant head saturation on the sandy soil sample by using the constant head deviceuntil the surface of the sandy soil sample is submerged by water; (6) after constant head saturation, exposing the sandy soil sample to atmosphere under a normal atmospheric pressure of P1; (7) vacuumizing the sandy soil sample again until the vacuum degree reaches P2; (8) measuring the change of liquid level heights of the sample container under different pressures in the steps (6) and (7), andobtaining the volume change ([delta]V); and (9) calculating the saturation (Sr) of the sandy soil sample based on a saturation formula.

The invention relates to a method for detecting a tumor mutant gene in blood. The method comprises the following steps of: (1) preparing a polyethylene oxide sieving medium containing TBE (Tetrabromoethane); (2) extracting a genome DNA (Deoxyribonucleic Acid) in a blood sample; (3) amplifying a gene target fragment needing to be detected: performing conventional PCR (Polymerase Chain Reaction) amplification by taking the genome DNA as an amplifying template to obtain a p53 gene PCR amplification sample and a k-ras gene PCR amplification sample; (4) treating the samples: performing denaturation or enzyme digestion on the p53 gene PCR amplification sample and the k-ras gene PCR amplification sample to obtain a denatured sample solution and an enzyme digestion solution respectively; and (5) detecting the samples: adding a 10*SYBR Green I fluorescence dye and the genome DNA into the denatured sample solution and the enzyme digestion solution respectively, uniformly mixing, adding deionized water till a system is 30-50 mu l, automatically filling the polyethylene oxide sieving medium by using a capillary electrophoresis apparatus pressure system and performing CE-SSCP (Capillary Electrophoresis-Single Strand Conformation Polymorphism) detection or CE-RFLP (Capillary Electrophoresis-Restriction Fragment Length Polymorphism) detection respectively. The method is easy to operate, and has high repeatability.

The invention discloses a voltage sag source identification method and identification device based on variational mode decomposition. The identification method comprises the following steps: acquiringan original voltage sag signal from an electric energy quality monitoring wave recording device; normalizing the original voltage sag signal to obtain an original time domain signal; carrying out variational modal decomposition on the original time domain signal to obtain K modal components; screening out a modal component IMF N which can represent different sag sources at the same time from theK modal components; and according to the waveform of the unique modal component of each type of sag source after the sag starting moment T of the modal component IMF N, corresponding to the characteristic of the unique modal component of each type of sag source, carrying out identification.

The invention discloses a method and a device for dynamic non-destructive measurement of CRTSII type plate-type ballastless track seam. The method comprises the following steps: firstly enabling an ultrasonic pulse transmitting receiver to generate a sine wave exciting signal modulated by a Hanning window, amplifying the exciting signal by a preamplifier to drive an air-coupled ultrasonic transmitting transducer to convert the electrical signal into an ultrasonic wave to hit a CRTSII type plate-type ballastless track plate, then adopting an air-coupled ultrasonic receiving transducer to collect a guided wave echo signal, amplifying the guided wave echo signal by a post-amplifier, subjecting to A / D conversion, transmitting to the ultrasonic pulse transmitting receiver, then transmitting thereceived guided wave echo digital signal to a computer by the ultrasonic pulse transmitting receiver, performing signal processing and analysis by the computer adopting MATLAB software, and judging whether a seam defect exists by comparing an obtained first wave amplitude with a preset threshold value. By adopting the method and the device provided by the invention, the high-efficiency, non-destructive and real-time measurement of the seam defect of a CRTSII type plate-type ballastless track can be realized.

The invention relates to a method for analyzing synergistic effect of aroma of an apple juice ester based on an S-curve method. The method is characterized by comprising a step of determining a relationship between the concentration Q of the apple juice ester and the detection probability P, in a simulated apple juice matrix solution, the key esters A and B and a binary mixture of A and B are taken as dilution factors for respectively determining the detection probability P (A), P(B), and P(AB) of esters A and B and the binary mixture of A and B under 10 gradients, a step of determining the synergistic effect of aroma between apple juice esters uses Sigma Plot 12.0 software to draft a curve of the material concentration Q-detection probability P for the obtained 10 data points, a linear correlation coefficient R is greater than 0.95, and fitting is carried out according to a formula; an S-curve model of the concentration and detection probability of different esters can be established,and the threshold value of the esters can be calculated; and according to the ratio between the measured threshold and the theoretical threshold, the synergistic effect of the aroma between the esters is determined.

The invention relates to a method for screening a drought-resistant corn germplasm and belongs to the field of crop genetics and breeding. The method for screening the drought-resistant corn germplasm includes the following steps that 1, sowing corn seeds with the corn germplasm, 2, conducting final singling of seedlings of corn plants, 3, treating the corn plants with drought stress, 4, detecting corn germplasm plant morphological indexes, 5, detecting corn germplasm yield indexes, 6, evaluating the drought resistance ability of the corn germplasm and 7, selecting the corn germplasm with the drought resistance. The method for screening the drought-resistant corn germplasm uses the corn germplasm as a detection object, the drought stress treatment is conducted in the whole growth period, by detecting the plant morphological indexes and the yield indexes, the purpose of building an objective and accurate method for screening the drought-resistant corn germplasm is achieved, and therefore a reliable basis is provided for drought-resistant corn germplasm screening.

The present invention belongs to the technical field of biomedicine, and particularly relates to an anti-oxidation drug screening kit and a use method thereof. According to the present invention, the anti-oxidation drug screening kit comprises Caenorhabditis elegans, a nematode culture medium, Escherichia coli OP50, and 2',7' dichlorofluorescin diacetate, and can be used for high-throughput screening of anti-oxidation drugs; and kit has characteristics of simple operation and rapid detection, and is suitable for the large-scale promotion in the field of anti-oxidation drug screening.

The invention discloses a molecular marker for identifying a fruit mulberry variety 'Guangdong mulberry 143', an identification primer group, a kit and application. The fluorescent SSR technology is utilized, 300 pairs of SSR primers designed and developed for the mulberry genome sequence are screened, it is confirmed that the core primers marked with M20541 and M22794 can amplify three specific bands for the new fruit mulberry variety 'Yuegui 143' respectively, the new fruit mulberry variety 'Yuegui 143' can be identified from the fruit mulberry strains respectively, and the specific bands of the new fruit mulberry variety 'Yuegui 143' and the specific bands of the new fruit mulberry variety 'Yuegui 143' can be identified from the fruit mulberry strains respectively. The method can be used for rapid identification and detection of a new fruit mulberry variety 'Guangdong mulberry 143'. According to the method, the M20541 marker and / or the M22794 marker of 'Yueyan mulberry 143' are / is directly detected to identify the 'Yueyan mulberry 143', the uncertainty of external morphological characteristics on identification of the 'Yueyan mulberry 143' is overcome, the operation is simple, the detection efficiency is high, the result is reliable and visual, a scientific technical guarantee is provided for protection of the new fruit mulberry 'Yueyan mulberry 143', and the method is beneficial to popularization, utilization and protection of the 'Yueyan mulberry 143'.