SSR (Simple sequence repeat) molecular marker of fruit mulberry variety 'Yue mulberry 74', core primer group of SSR molecular marker, kit and application

A technology of molecular markers and core primers, which is applied in the identification of variety resources and germplasm innovation, can solve problems affecting the effectiveness and authority of new variety identification, large environmental impact, and long test cycle, and achieve reliable and intuitive results. The effect of high efficiency and overcoming uncertainty

Active Publication Date: 2021-06-18
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

[0005] The traditional identification of new mulberry varieties is mainly based on phenotypic traits, which is greatly affected by the environment, poor stability, and long testing cycle, which seriously affects the effectiveness and authority of new variety identification

Method used

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  • SSR (Simple sequence repeat) molecular marker of fruit mulberry variety 'Yue mulberry 74', core primer group of SSR molecular marker, kit and application
  • SSR (Simple sequence repeat) molecular marker of fruit mulberry variety 'Yue mulberry 74', core primer group of SSR molecular marker, kit and application
  • SSR (Simple sequence repeat) molecular marker of fruit mulberry variety 'Yue mulberry 74', core primer group of SSR molecular marker, kit and application

Examples

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Embodiment 1

[0033] Example 1 Obtained by Screening of Specific Primer Sequences of New Fruit Mulberry Variety 'Yuechen 74'

[0034] 1. Selection of SSR primers

[0035] Use MISA software to search the genome sequence in the mulberry genome database, and retrieve the SSR sites in the genome sequence. The length of the retrieved SSR motif repeating unit is 2 to 6 nucleotides, and set 2, 3, and 4 respectively , 5, 6 nucleotides, the minimum number of search repetitions is 6, 5, 5, 4, 4 times, and the length of the flanking sequence of the SSR site is ≥ 150 bp, according to the searched SSR site, use Primer3v2. 3. 4 (http: / / primer3.sourcego-rge.net) software to design SSR primers, the design criteria are: the length of the primer is 18-25bp, the annealing temperature is 57-63°C, the GC content is 40-70%, the length of the PCR product 100-300bp, randomly select SSR sites that can be compared with the NCBI non-redundant protein database to synthesize 200 pairs of primers, each pair of primers ...

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Abstract

The invention discloses an SSR (simple sequence repeat) molecular marker of a fruit mulberry variety 'Yue mulberry 74', a core primer group of the SSR molecular marker, a kit and an application. A fluorescent SSR technology is utilized, 200 pairs of SSR primers developed through mulberry genome sequence design are screened, it is confirmed that M792 and M20197 marked core primers can amplify two specific bands for the new fruit mulberry variety 'Yue mulberry74' respectively, and the new fruit mulberry variety 'Yuemulberry 74' can be identified from fruit mulberry strains respectively. The molecular marker can be used for rapid identification and detection of the new fruit mulberry variety 'Yue mulberry 74'. The M792 marker and / or the M20197 marker of 'Yue mulberry 74' are / is directly detected to identify 'Yue mulberry 74', the uncertainty of external morphological characteristics on identification of 'Yue mulberry 74' is overcome, the operation is simple, the detection efficiency is high, the result is reliable and visual, a scientific technical guarantee is provided for protection of the new fruit mulberry variety 'Yue mulberry 74', and popularization, utilization and protection of the variety are facilitated.

Description

technical field [0001] The invention relates to the technical field of identification of variety resources and germplasm innovation, in particular to the SSR molecular marker of a fruit mulberry variety 'Yuechen 74' and its core primer set, kit and application. Background technique [0002] Mulberry is the Moraceae plant mulberry ( Morus alba The mature ear of L.) belongs to the category of berries. Mulberry fruit is becoming more and more popular with people because of its unique taste, rich nutrition and high content of active substances. It has become the representative of the third-generation fruit and has been listed as "both food and medicine" by the Ministry of Health. . In addition to fresh food, mulberries are processed and developed into mulberry juice, mulberry wine, mulberry jam, mulberry red pigment, mulberry vinegar and other products. The mulberry industry has begun to take shape and has shown a good momentum of diversified development. [0003] The new fru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6895C12Q2600/156C12Q2531/113C12Q2525/151C12Q2563/107C12Q2565/125
Inventor 王振江唐翠明罗国庆戴凡炜钟建武林森陈莲李智毅
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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