225results about How to "Implement parallel detection" patented technology

The invention discloses a portable document format (PDF) document form identification method. The method comprises the steps of acquiring character sets in a page, combining the character sets in rows, and establishing a row set; extracting horizontal lines and vertical lines in a page path, and establishing a line set; detecting suspected form titles in the row set and suspected form lines in the line set; if both the suspected form titles and the suspected form lines exist, identifying the form by using a region growth method based on the form titles and the line set; if only the suspected form lines exist, firstly detecting an all line form and then detecting a three line form by using the line set and the row set; if only the suspected form titles exist, identifying the form by using a region growing method based on the form titles and the row set; if neither the suspected form lines nor the suspected form titles exist, determining that the page has no form; and detecting form attached elements like a form header, form notes and so on, and outputting a form identification result of the page. According to the method, the form title, form lines and form character arrangement characteristic are deemed as three characteristics of the form, and the form can be located accurately in a complicated page layout with multiple forms on one page by adopting the method of regional parallel growth.

The invention discloses a liquid phase chip for CYP19A1 gene SNP (Single Nucleotide Polymorphism) detection, which comprises wild type and mutant type specific ASPE primers designed targeting CYP19A1 gene SNPs of rs4646, rs10046C>T, rs700519C>T, rs1870050C>A, hCV1664178A>C, rs12900137G>C, rs730154G>A, rs936306T>C and rs1902586A>G, microballoon spheres respectively coated with specific anti-tag sequences and primers used for amplifying CYP19A1 gene SNPs with target sequences to be detected. Each ASPE primer comprises a tag sequence at 5' end and a specific primer sequence at 3' end, wherein the specific primers are selected from SEQ ID NO. 19-36 and the tag sequences are selected from SEQ ID NO.1-18; and the anti-tag sequences can be correspondingly in complementary pairing with the tag sequences. The invention also discloses a CYP19A1 gene SNP detection method. The coincidence rate of the detection method provided by the invention and a sequencing method is as high as 100%; and the prepared liquid phase chip for CYP19A1 gene SNP (Single Nucleotide Polymorphism) detection has excellent signal-to-noise rate, and basically no cross reaction exists between a design probe and the anti-tag sequences.

The embodiment of the invention provides a radio frequency front end circuit of a mobile terminal and a whole machine coupling testing method. The radio frequency front end circuit comprises a master antenna, a diversity antenna, a diversity antenna switch and a first power detection circuit, wherein the diversity antenna uses coupling effects between the master antenna and the diversity antenna and receives testing signals transmitted by the master antenna; the input end of the diversity antenna switch is connected with the diversity antenna for receiving the testing signals from the diversity antenna; the output end of the diversity antenna switch is used for outputting the testing signals to the first power detection circuit; and the first power detection circuit is used for carrying out power detection on the testing signals to realize whole machine coupling test on the mobile terminal. Thus, dependence on an integrated test instrument can be eliminated, and the hardware cost in the case of whole machine coupling test is reduced.

The invention discloses a specific primmer, a liquid phase chip and a detection method for CYP2E1 (Cytochrome P450 2E1) gene SNP (Single Nucleotide Polymorphism) detection. The liquid phase chip comprises wild-type and mutant ASPE (Allele-Specific Primer Extension) primer pairs respectively designed to each type of mutant site, microspheres respectively encapsulated with specific anti-tag sequences, and primers for respectively amplifying a CYP2E1 gene target sequence with G1168A, C1053T and/or G1293C SNP site. The prepared liquid phase chip for CYP2E1 gene SNP detection has excellent signal-to-noise ratio, and the designed probe and the anti-tap sequence do not exit the cross reaction basically. The ASPE primers have excellent specificity and can exactly distinguish all kinds of mutant sites. The detection method has simple steps, the detection of three kinds of SNP bits can be finished once with simple operation, and a plurality of undetermined factors existing in multiple operation processes are avoided, thus the precision rate of the detection can be greatly improved.

The invention provides a CYP2D6 gene mutation detection liquid-phase chip which comprises ASPE (Allele Specific Primer Extension) primers aiming at CYP2D6 C2850T and CYP2D6Deletion mutational sites, three microballoons respectively enveloped with a specific anti-tag sequence and amplification primers aiming at the CYP2D6C2850T and the CYP2D6 Deletion mutational sites. The CYP2D6 gene mutation detection liquid-phase chip can simultaneously detect aiming at the CYP2D6 C2850T and the CYP2D Deletion mutational sites and has excellent signal to noise ratio. The coincidence ratio with a sequencing method of the CYP2D6 gene mutation detection liquid-phase chip reaches up to 100 percent, and the CYP2D6 gene mutation detection liquid-phase chip has higher specificity and precision compared with intra-class correlation products.

The invention relates to a Heliostat surface shape on-site detecting system and method. The heliostat surface shape on-site detecting system at least comprises an image collecting system, an installing support of the image collecting system, a calculating server and a heliostat control server, the image collecting system is fixed to a support through the installing support, the visual field of theimage collecting system covers multiple sets of target heliostats in a heliostat field, and the image collecting system is used for collecting heliostat surface images of the target heliostats underdifferent postures; the calculating server is used for receiving image data of the image collecting system, and then a mathematical algorithm and an image plane calibration method are used for workingout normal vectors of all regions of the target heliostats, obtaining heliostat surface shape information of the target heliostats and storing the heliostat surface shape information into a heliostatparameter database of the heliostat control server; the heliostat control server automatically calculates an operation statement used for a surface shape used for detecting according to parameters ofthe target heliostats in the heliostat parameter database, and controls the target heliostats to rotate according to the operation statement, so that the image collecting system obtains the heliostatsurface images of the heliostats under different preset postures.

The invention discloses an NRAS gene mutation detection specificity primer and a liquid chip thereof. The liquid chip mainly comprises: an ASPE primer composed of a 5'-terminal tag sequence and 3'-terminal specificity primer sequences focused on target gene mutation sites, wherein the specificity primer sequences comprise SEQ ID NO.18, SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21 and/or SEQ ID NO.22 focused on a Codon12 site, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, SEQ ID NO.26 and/or SEQ ID NO.27 focused on a Codon13 site, SEQ ID NO.28 and SEQ ID NO.29 focused on a Codon18 site, and/or SEQ ID NO.30, SEQ ID NO.31, SEQ ID NO.32, SEQ ID NO.33 and/or SEQ ID NO.34 focused on a Codon61 site; a microsphere coated by an anti-tag sequence; and an amplimer. The consistency between the detection result of the detection liquid chip provided by the invention and the detection result of a sequencing method is high to 100%, and the wild-type and mutant parallel detection of a plurality of mutation sites is realized.

The invention discloses a THADA gene mutation detection liquid phase chip and a specific primer. The liquid phase chip mainly comprises: an ASPE primer composed of a tag sequence at 5'-terminal and specific primer sequences against the mutation site of a target gene at 3'-terminal, wherein the specific primer sequences comprise SEQ ID NO.13 and SEQ ID NO.14 against an A109G site, SEQ ID NO.15 and SEQ ID NO.16 against a T156C site, SEQ ID NO.17 and SEQ ID NO.18 against an A89C site, SEQ ID NO.19 and SEQ ID NO.20 against a G153A site, SEQ ID NO.21 and SEQ ID NO.22 against a G162T site, and/or SEQ ID NO.23 and SEQ ID NO.24 against a C80T site; a microsphere coated with an anti-tag sequence; and an amplimer. The coincidence rate between the detection result of the detection liquid phase chip and a sequencing method is 100%, and the wild and mutant parallel detection of a plurality of mutation sites can be realized.

The invention discloses a respiratory tract pathogenic microorganism detection primer combination, a kit and a method, and belongs to the technical field of microorganism detection. The respiratory tract pathogenic microorganism detection primer combination comprises primers with nucleotide sequences as shown in SEQ ID No.1 to SEQ ID No.20 respectively. According to the method, aiming at a clinical suspected respiratory tract infection sample, a multiplex PCR technology is combined with a targeted sequencing technology to analyze common pathogenic microorganisms in the sample, and the method can be used for detecting pathogenic microorganisms such as bacteria, fungi, viruses (DNA and RNA viruses), mycoplasmas and chlamydia, is simple, rapid and accurate, and is beneficial to clinical precise diagnosis and treatment.

The invention discloses an SNP (Single Nucleotide Polymorphism) detection liquid phase chip for KIF6 gene, mainly comprising an ASPE (Application Solid Phase Extraction) primer pair, microballons and an amplification primer, wherein each ASPE primer consists of a 5'-end tag sequence and 3'-end specific primers aiming at T2155C SNP locus; the 3'-end specific primers comprise SEQID NO.23 and SEQ IDNO.24; the tag sequence is selected from SEQID NO.1 to SEQID NO.18; and the microballons are respectively coated by a specific anti-tag sequence and have codes in different colors. The invention also discloses the SNP detection liquid phase chip for apoE and KIF6 genes and the chromosome 9p21 section. The occlusion rate of the detection method and the sequencing method which are provided in the invention is as high as 100 percent. The prepared SNP detection liquid phase chip for the apoE and KIF6 genes and the chromosome 9p21 section has favorable single-noise ratio.

The invention discloses a soft error real-time error detection and recovery method and system for online parallel processing. The method comprises the following steps: dividing a protected RAM space into a plurality of protected areas; dividing all protected areas into one or more levels, wherein the highest level is to complete one error detection and recovery function for each interruption period, wherein other levels are multiple interrupt periods to complete one-time error detection and recovery function; registering the protected area of each level to generate a linked list correspondingto the number of levels, and backing up at least two parts of each linked list and the protected area in the linked list to other RAM spaces; and performing parallel processing on each level of the linked list and error detection and recovery of each protected area in the linked list. According to the method, high-importance-level data can be verified, judged, corrected and recovered in a single interrupt beat of a control system in a key scene, and the method does not depend on a CPU processor, and can perform real-time parallel processing; and the method can achieve the online real-time error detection and correction function when multiple positions of the RAM memory of the CPU are abnormally shifted at the same time.

The invention discloses an ATM gene mutation detection specific primer and a liquid chip. The liquid chip mainly comprises ASPE primers consisting of tag sequences at a 5' end and specific primer sequences aiming at target gene mutation sites at a 3' end, microspheres coated with anti-tag sequences and an amplification primer, wherein the specific primer sequences are as follows: SEQ ID NO.13 and SEQ ID NO.14 which aim at a T2572C site, SEQ ID NO.15 and SEQ ID NO.16 which aim at an A7325C site, SEQ ID NO.17 and SEQ ID NO.18 which aim at a G7328A site, SEQ ID NO.19 and SEQ ID NO.20 which aim at a C9022T site, SEQ ID NO.21 and SEQ ID NO.22 which aim at a G9023A site, and/or SEQ ID NO.23 and SEQ ID NO.24 which aim at a C9139T site. The detection liquid chip provided by the invention has the advantages that the matching rate between the detection result and a sequencing method is up to 100%, and the wild-type and mutant parallel detection of multiple mutant sites can be realized.

The invention provides a single molecule DNA fluorescence signal detection system. The system includes an array chip and an optical detection structure; the array chip is arrayed with multiple array micro-pores and integrated with multiple light-emitting members; and the optical detection structure collects fluorescence signals and converts the fluorescence signals into digital signals so as to realize the detection of single molecule DNAs. A detection method for the array micro-pores is also related. Through the integration of the light-emitting members in the microporous array, the mode of zero-mode waveguide lighting can be avoided, the utilization rates of excitation light can be increased, fluorescence excitation efficiency can be enhanced, and the fluorescence signals can be strengthened; compared with existing blind hole structures of zero-mode waveguides whose bottoms are transparent materials, the loss of light signals passing optical elements can be reduced, and the accuracyrates of fluorescence signal detection and identification can be enhanced; and the size limit of zero-mode waveguides wells can be avoided, and sequencing microporous array chips with higher throughput can be applied, so that single molecule fluorescence sequencing can be realized.

The invention discloses a specificity primer and a liquid phase chip for SNP detection of CYP4F2 gene. The liquid phase chip comprises an ASPE primer formed by the specificity primer aiming at a target gene SNP locus of the tag sequence on the 5' end and 3' end, wherein the specificity primer is SEQ ID NO.13 and SEQ ID NO.14 aiming at the G1297A SNP locus and/or SEQ ID NO.15 and SEQ ID NO.16 aiming at the T34G SNP locus, a microsphere and an amplification primer. The invention also provides a liquid phase chip for SNP detection of CYP4F2 and EPHX1 genes, which comprises the corresponding compositions of the liquid phase chip for the SNP detection of the CYP4F2 and EPHX1 genes, an ASPE primer pair aiming at the G357A, G19512990A, T337C and/or A416G SNP locus of the EPHX1 gene, microsphere and amplification primer. The detection liquid phase chip has an excellent signal-noise ratio, can avoid cross reaction and can realize the parallel detection of a plurality of SNP loca.

The invention relates to the field of bioelectronics and discloses an addressable semiconductor disease chip and a manufacturing method thereof. The semiconductor disease chip comprises a semiconductor chip and detection wells. The invention also relates to a surface functionalization method and a detection method. The semiconductor chip is characterized by comprising at least one sensor array and a detection well array above the sensors correspondingly, wherein the sensors are connected through a circuit; the positions of all the wells are confirmed and the wells are not interfered from each other.Detection indexes for specific diseases are fixed in the wells; target molecules enter into the detection wells and can have special reactions; corresponding enzymes can catalyze substrates for changing ion concentration; the sensors can sense the pH change in the detection wells so as to generate an electric signal. One well at most is corresponding to one index or one disease or a plurality of wells are corresponding to different indexes of one disease, and thus the multi-index simultaneous detection can be realized. The position of strong signal is corresponding to the well with active reaction, namely, to the to-be-detected index in the well.