The invention discloses an apple fruit key
aroma substance identification method based on sensory
omics, and relates to the technical field of
aroma substance detection, and the method specifically comprises the following steps: S1, detecting volatile compounds in apple fruits through comprehensive two-dimensional
gas chromatography-time-of-flight
mass spectrometry (GC * GC-QTOFMS); s2, carrying out
gas chromatography-olfactory analyzer (GC-O) and
odor activity value (OAV) analysis to characterize characteristic
aroma compounds in the apple fruits; and S3, determining key characteristic aroma compounds in the apple fruits through aroma recombination and omission experiments. According to the present invention, the aroma substance extraction and enrichment are performed on the apple by using the
solid phase micro-extraction, the
organic solvent is not required, the operation is simple and easy, the comprehensive two-dimensional
gas chromatography-tandem time-of-flight
mass spectrometry can comprehensively analyze the volatile compounds in the apple fruit within the short time compared with the common
chromatography-
tandem mass spectrometry, the peak capacity is significantly improved, and the determination result is significant. The separation and analysis efficiency is obviously superior to that of a traditional gas chromatograph-
mass spectrometer.