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Method for analyzing and identifying key aromatic substances in tobacco leaf

A technology for aroma-causing substances and tobacco leaves, applied in the field of tobacco, can solve the problem that the instrument detection technology cannot discriminate the aroma contribution of aroma compounds, and achieve the effect of good technical guidance and data support

Inactive Publication Date: 2012-09-19
SHANGHAI TOBACCO GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Instrument detection technology is mainly based on GC-MS, LC-MS, etc., mainly for qualitative and quantitative analysis of substances, but it is not the substances with large content that play an important role in the aroma of tobacco leaves; sensory evaluation refers to the evaluation of aroma components in tobacco leaves. The overall irritation of the nasal cavity and oral cavity of the inhaler, while the instrumental detection technology cannot distinguish the aroma contribution of the aroma compound

Method used

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  • Method for analyzing and identifying key aromatic substances in tobacco leaf
  • Method for analyzing and identifying key aromatic substances in tobacco leaf
  • Method for analyzing and identifying key aromatic substances in tobacco leaf

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The following experimental methods were used to analyze the aroma-inducing substances in flue-cured tobacco leaves:

[0037] (1) Use 15ml of anhydrous ether as a solvent to extract the aroma-causing substances in 500mg of tobacco powder, and add 3ml of tartaric acid solution to remove nicotine. The solution was concentrated to 500 μL;

[0038] (2) Using GC-MS / O to analyze and identify key aroma substances in tobacco leaves: inject the concentrated solution of key aroma substances obtained in step (1) into GC-MS / O, and the concentrated solution enters the gas chromatograph and passes through Y Half of the tube enters the mass spectrometer, while the other half enters the ODP instrument to obtain GC spectra, MS total ion current diagrams, aroma intensity diagrams and FD factor diagrams respectively; image 3 shown.

[0039] GC-MS conditions: GC analysis detection conditions: GC inlet temperature 250°C; sample volume: 3.0 μL; splitless injection; carrier gas is helium, a...

Embodiment 2

[0053] Aroma-inducing substances in Burley tobacco leaves were analyzed by the same experimental method as flue-cured tobacco leaf samples. GC-MS / O analysis results of Burley tobacco leaves (such as Figure 4 and Table 2) as follows:

[0054] Among the 15 aroma active compounds in Burley tobacco leaves, the intensity is the largest, and the compound that contributes the most to the aroma of tobacco leaves is β-Damascenone and an unknown substance (number 6) with a fresh green aroma, both of which have an intensity of 4 , β-Damascenone is sweet, honey, rose, and pleasant. Substances with relatively small intensity include methyl eugenol, dihydroactinolactone and 2 unknown substances (serial numbers 24 and 30). Fragrance, cool breath, their intensity is 3. There are many compounds with an aroma intensity of 2, and there are 7 compounds in total, namely solanone, β-damascone, geranyl acetone, macrostigmatrienone 4 and 3 unknown substances (numbered 8, 10, 12 ), the aroma of s...

Embodiment 3

[0062] The same experimental method as that of flue-cured tobacco leaves was used to analyze the aromatizing substances in oriental tobacco leaves. GC-MS / O analysis results of oriental tobacco leaves (such as Figure 5 and Table 3) as follows:

[0063] Fourteen aroma-active compounds were perceived by sniffers in oriental tobacco leaves. Among them, the compound with the highest aroma intensity is β-Damascenone, with an intensity of 4, which has a sweet, honey, rose, and pleasant smell. There are 6 aroma compounds with an intensity of 3, namely methyl eugenol, dihydroactinolactone and 4 unknown compounds (numbered 6, 8, 9, 27). Methyl eugenol has frankincense, fruity, milky, comfortable smell, and dihydroactinolactone has green and cool smell. The odors of the 4 unknown compounds are green, irritating, fresh, green, irritating, unpleasant, weakly irritating green, sour, woody, spicy, and powdery. The aroma compound with an intensity of 2.5 is macrostigmatrienone 4, which h...

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Abstract

The invention belongs to the technical field of tobacco, and relates to a method for analyzing and identifying the key aromatic substances in tobacco leaf. The method combines the instrument detection technology and a smelting method, wherein a GC (Gas Chromatograph) can qualitatively analyze the aroma compounds, an MS (Mass Spectrometer) can quantitatively analyze the content of each aroma compound in the tobacco leaf, and an ODP (olfactometry detection port) in combination with the smelling of the sensory organ can obtain the odor intensity and odor type of each aroma compound. The invention provides a quick and simple analysis method for identifying the key aromatic substances in the tobacco leaf; the method can quickly and accurately find the compounds in the tobacco leaf greatly contributing to the aroma of the tobacco leaf, and judges the aroma type and odor intensity of the key aromatic substances of the tobacco leaf; and the invention provides perfect technical guidance and data support for the flavoring and blending of the cigarette.

Description

technical field [0001] The invention belongs to the technical field of tobacco, and in particular relates to an analysis and identification method of key aroma-generating substances in tobacco leaves. Background technique [0002] There are great differences in the odor threshold of different substances, and substances with very low content often have a greater contribution to aroma due to their low threshold. At present, the determination methods of aroma substances in tobacco leaves are mainly instrument detection technology or sensory evaluation. The instrument detection technology is mainly based on GC-MS, LC-MS, etc., mainly for qualitative and quantitative analysis of substances. However, it is not the substances with large content that play an important role in the aroma of tobacco leaves; sensory evaluation is the evaluation of aroma components in tobacco leaves. The overall stimulation of the nasal cavity and oral cavity of the inhaler, while the instrumental detec...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 谢雯燕高建宏张士成刘百战
Owner SHANGHAI TOBACCO GRP CO LTD
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