The invention belongs to the material test technical field, to be more exactly, relates to a fatigue test sample clamp with high temperature resistance, high pressure resistance and aqueous corrosion resistance and an usage method thereof, which solves the problems of high temperature irresistance, high pressure irresistance, aqueous corrosion, large screw thread slit and poor centering in the current sample clamp. The clamp is provided with a column, a platform, an upper press cap, a lower press cap and a conversion adapter; the lower part of the column of the clamp is fixed on a cover of anautoclave, the upper part of the column of the clamp is fixed on the platform, and respectively and tightly pressed by lock nuts, the lower part of the conversion adapter is connected with a stretching shaft of a fatigue machine, through elevating the stretching shaft, an upper convex plate and a lower convex plate of the test sample are respectively placed in a platform groove and a conversion adapter groove, two dimidiate semirings are pressed on upper convex plate and the lower convex plate of the test sample, the upper press cap and the lower press cap are sleeved, the press cap bolts arescrewed for fixing two ends. The fatigue test sample clamp can be used in the autoclave for carrying out various fatigue tests (stress-stress, stress-tension, tension-tension and the like) under the high temperature high pressure solution environment, and has the advantages of simple structure and low cost.