The application provides application of pig's tooth
saponin volatile oil in preparation of a
medicine for treating cerebral
ischemia-
reperfusion injury, and a preparation method of the pig's tooth
saponin volatile oil is as follows: pig's tooth
saponin decoction pieces are taken, water and n-
hexane are added, and volatile oil is obtained through
steam distillation; the volatile oil is collected,
anhydrous sulfuric acid is added, and water is removed; and the mixture is left overnight to obtain the pig's tooth saponin volatile oil. The prepared pig's tooth saponin volatile oil acts on key target points of VEGFA, SRC, MAPK8, PIK3CA and TNF through active ingredients of
paeonol,
linalool,
methyl eugenol,
anethole and
eugenol, regulates multiple
signal pathways of sphingolipids, TNF and VEGF, inhibits
cell oxidative stress injury, inhibits release of
inflammatory factors and regulates
nerve function, so as to play a role in treating cerebral
ischemia-
reperfusion injury, and lay a foundation for further in-depth study on a treatment mechanism of pig's tooth saponin in treating 'opening the orifices and connecting the heart and the
lung'.