Method for measuring methyl eugenol in mainstream smoke of cigarettes

A technology for mainstream cigarette smoke and methyl eugenol, which is applied in the field of measuring methyl eugenol in mainstream cigarette smoke, can solve problems such as undiscovered methyl eugenol detection, achieve good economic and social effects, and ensure accurate Sensitivity and sensitivity

Active Publication Date: 2014-02-05
CHINA TOBACCO JIANGSU INDAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, no corresponding method for detecting methyl eugenol in cigarette smoke has been found in China

Method used

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  • Method for measuring methyl eugenol in mainstream smoke of cigarettes
  • Method for measuring methyl eugenol in mainstream smoke of cigarettes
  • Method for measuring methyl eugenol in mainstream smoke of cigarettes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A method for measuring methyl eugenol content in cigarette mainstream smoke, comprising the following steps:

[0040] (1) Configuration of internal standard solution

[0041] Primary internal standard stock solution: accurately weigh 0.1123g of anethole, and dilute to 100mL with ethanol, with a concentration of 1.123mg / mL; secondary internal standard stock solution: accurately pipette 1mL primary internal standard solution, and dilute to volume with ethanol (3) Internal standard solution: Accurately pipette 1 mL of secondary internal standard stock solution, and dilute to 10 mL with ethanol, with a concentration of 1.123 μg / mL.

[0042] (2) Preparation of standard working solution

[0043] Primary standard stock solution: Accurately weigh 0.1050g methyl eugenol, dilute to 100mL with ethanol, the concentration is 1.05mg / mL; (2) Secondary standard stock solution: accurately pipette 1mL standard stock solution, dilute with ethanol (3) Standard series solutions: Pipette 1...

Embodiment 2

[0064] Taking a cigarette sample as the analysis object, a precision test was carried out, and a total of 6 measurements were taken, and the relative standard deviation (RSD) of the 6 parallel measurement results were calculated respectively. The measurement results are shown in Table 2 below. The results in the table show that the RSD of the repeatability of the experimental method is less than 7%. For the analytical detection of trace substances, the method has good precision.

[0065] The precision of the method in Table 2

[0066] serial number Determination result (ng / branch) 1 8.64 2 9.12 3 7.87 4 9.08 5 7.85 6 8.97 RSD (%) 6.86

[0067] Taking a cigarette sample as an object, carry out standard addition treatment at three different concentration levels of low, medium and high, and then process according to the above standard pretreatment steps, and then conduct GC-MS analysis to calculate the recovery rate of the stan...

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Abstract

The invention discloses a method for measuring methyl eugenol in the mainstream smoke of cigarettes. The method comprises the steps of preparation of an internal standard solution, preparation of a standard working solution, preparation of a sample solution, gas chromatography-tandem mass spectrometry, drawing of a standard working curve and calculation of a sample result. According to the method, a C18 solid-phase extraction column is used for purifying sample extract for the first time, a dehydration technology is used for carrying out back-extracting on a target object, and then a gas chromatography-mass spectrometry selective ion monitoring method is used for measuring the methyl eugenol content of the mainstream smoke of the cigarettes; an internal standard method is used for quantification, so that constant column is not required, and errors caused by the reproducibility of the pretreatment method and precision problems of an apparatus can be reduced; a DB-5MS column with the best peak shape and the best separation effects is selected as a separation column, and GC-MS (gas chromatography-mass spectrometry) detection mode capable of simultaneously providing qualitative and quantitative information is adopted, so that the sensitivity of a response signal is improved, and the accuracy of an analysis result is ensured.

Description

technical field [0001] The invention belongs to the technical field of cigarette mainstream smoke index measurement, and in particular relates to a method for measuring methyl eugenol in cigarette mainstream smoke. Background technique [0002] Methyl eugenol, also known as eugenol methyl ether, L-rose ether, eugenol methyl ether, O-methyl eugenol, 4-allyl veratrol, 4-allyl veratrol, eugenyl methyl Methyl Eugenol (Eugenyl Methyl Ether). CAS number is 93-15-2, molecular formula is C11H14O2, molecular weight is 178.23, colorless to slightly yellow liquid. Boiling point 249°C, relative density 1.032-1.036, refractive index 1.532-1.536, flash point 99°C, soluble in ethanol and oil. There is a sweet clove-fennel spicy aroma, like carnation, the aroma is more transparent and long-lasting, and it has a mild spicy aroma like tea. According to the classification of toxicity grade, methyl eugenol is poisoned. According to MSDS data, its LD50 (rat, oral) is 1179 mg / kg, and LD50 (mi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
Inventor 廖惠云庄亚东曹毅王珂清李朝建熊晓敏石怀彬刘献军张媛朱莹朱龙杰韩开冬
Owner CHINA TOBACCO JIANGSU INDAL
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