The invention relates to an extraction process for industrial
mass production of
spinosad. The process includes the steps of:
fermentation broth pretreatment, frame and plate
filtration, and flash
drying;
mycelium soaking in
methanol; concentration of extract liquid,
phase inversion washing; back extraction with
tartaric acid; adjustment of pH and
centrifugation to obtain a
spinosad crude product; and recrystallization with
methanol to obtain a
spinosad fine product. The process provided by the invention extracts spinosad by means of
mycelium separation,
drying and extraction, and has the characteristics of high extraction efficiency, small
solvent dosage, which is only half of that of
fermentation broth direct
solvent extraction, relatively low
energy consumption, and shortened
production cycle. In addition, the equipment needed by frame and plate
filtration, flash
drying, extraction, concentration, washing, back extraction and refining is general, therefore industrial production can be realized easily. The obtained product has high yield up to 85%-90%, after purification of spinosad coarse crystals by
solvent recrystallization, the quality of spinosad is improved, and the product content reaches 95%-98%.