326 results about "Isoamyl acetate" patented technology

In vivo method of producing esters from acetyle coA, such as isoamyl acetate and succinate, has been developed by producing null mutants in pathways that use acetyl coA and by overexpressing products that use NADH and in order to maintain the proper redox balance between NADH and NAD+. The method is exemplified with null mutations in ldhA, adhE, ackA-pta and overexpression of pyruvate carboxylase and alcohol acetyltransferase. This strain produces higher levels of both isoamyl acetate and succinate.

In vivo method of producing esters from acetyle coA, such as isoamyl acetate and succinate, has been developed by producing null mutants in pathways that use acetyl coA and by overexpressing products that use NADH and in order to maintain the proper redox balance between NADH and NAD+. The method is exemplified with null mutations in ldhA, adhE, ackA-pta and overexpression of pyruvate carboxylase and alcohol acetyltransferase. This strain produces higher levels of both isoamyl acetate and succinate.

The invention discloses a saccharomyces cerevisiae strain with high yield of ester and low yield of higher alcohol as well as a building method of the saccharomyces cerevisiae strain, and belongs to the technical field of bioengineering. According to the building method provided by the invention, through completely knocking out an amino acid transaminase gene BAT2 and an ester hydrolase gene IAH1 in an original strain, and selecting a strong promoter PGK1 over-expression alcohol acetyltransferase I gene ATF1 at the same time, the saccharomyces cerevisiae strain with high yield of ester and low yield of higher alcohol is obtained. Compared with a parent strain, other fermentation performances of built recombinant bacteria are not affected, the total quantity of acetic acid ester is obviously increased and reaches 1303.6mg / L, wherein the content of ethyl acetate is 52 times that of the original strain, isoamyl acetate is increased to 73.7mg / L, the content of main higher alcohol is 151.8mg / L and is reduced by 61.4 percent in comparison with that of the original strain. By using the saccharomyces cerevisiae, ester yield is significantly increased while the higher alcohol yield is reduced, the higher requirements of white spirit related fields on yeast are met and the application prospect is wide.

The invention provides a transparent hydrophobic and oleophobic functional coating material. The transparent hydrophobic and oleophobic functional coating material is prepared from fluorine-containing resin, silicon dioxide particles and a solvent, wherein the mass concentration of the fluorine-containing resin is 5 to 15%; the mass of the silicon dioxide particles accounts for 1 to 5% of the mass of the fluorine-containing resin; the fluorine-containing resin contains at least one of fluorine-containing acrylic resin, fluorocarbon resin, fluorine-containing acrylic modified polyurethane resin and fluorine-containing acrylic modified organic silicon resin; the fluorine content of the fluorine-containing resin is 10 to 50wt%, the particle size of the silicon dioxide particles is 10 to 40 nanometers, and a modifying solvent uses a fluoroalkyl silane coupling agent to modify; the solvent is prepared from ethyl acetate, butyl acetate, isoamyl acetate, ethyl butyrate, acetone, butanone, cyclohexanone, methylbenzene, xylene or nonafluorobutyl ether. The invention also provides a transparent hydrophobic and oleophobic functional coating layer which is made of the transparent hydrophobic and oleophobic functional material.

The invention discloses Saccharomyces cerevisiae genetic engineering bacteria with high ester yield. The Saccharomyces cerevisiae genetic engineering bacteria EY-13 were collected in China General Microbiological Culture Collection Center on November 17, 2010, the collection number is CGMCC NO.4350, and the Saccharomyces cerevisiae genetic engineering bacteria are recommended to be named Saccharomyces cerevisiae. PGK1 derived from the Saccharomyces cerevisiae is selected as a promoter; and the construction method of the Saccharomyces cerevisiae genetic engineering bacteria with the high esteryield comprises a step of knocking-out an IAH1 gene for encoding ester hydrolase in a Saccharomyces cerevisiae genome when an ATF1 gene which is derived from the Saccharomyces cerevisiae and is used for encoding alcohol acetyltransferase is overexpressed. Compared with initial recipient bacteria, the Saccharomyces cerevisiae genetic engineering bacteria have the advantages that: after rice wine fermentation is simulated, the content of isoamylol is about one half, the content of ethyl acetate is improved by 20 times, the content of isoamyl acetate is 100mg / L, and the content of isobutyl acetate is 5 to 7mg / L; and after liquor fermentation is simulated, the content of total esters is improved by 4 times and the content of the ethyl acetate is improved by 35 times, so an excellent strain is provided for the production of brewing industry.

The present invention provides a flavoured product comprising four or more flavour materials having antimicrobial properties and selected from the group comprising nonanol, decanol, nonanal, decanal, amyl propionate, anethole synthetic, anisic aldehyde, basil oil, benzyl benzoate, benzyl butyrate, benzyl formate, camomile oil, cinnamic aldehyde, cis-3-hexenol, clove bud oil, damascone, ethyl acetoacetate, eucalyptus oil, ginger, isoamyl acetate, menthol laevo, methyl cinnamate, methyl salicylate, orange oil, rosemary oil, tarragon, Tea Tree oil, and peppermint oil; and one or more antimicrobial agents selected from the group comprising triclosan, pyrophosphates, zinc salts, cetylpyridinium chloride, parabens, stannous salts, sodium dodecyl sulphate, chlorhexidine, copper salts, strontium salts, peroxides and sanguinarine.

The invention provides a selective plating method for an aluminum material, comprising the following steps of: pre-treating the surface of the aluminum material, wherein the surface of the aluminum material comprises a non-plated region and a region to be plated; carrying out conductive oxidation treatment on the non-plated region on the pre-treated surface of the aluminum material so as to form a conductive oxidation layer on the non-plated region; spraying the non-plated region of the aluminum material which is subjected to the conductive oxidation treatment, and drying after a protective coating is formed; plating the dried aluminum material so as to form a plated layer on the region to be plated; and dissolving and removing the protective coating with isoamyl acetate. According to the selective plating method for the aluminum material, the non-plated region of the aluminum material is protected and shielded very strictly by means of spraying the coating; and the coating is dissolved and removed with the isoamyl acetate after the region to be plated is plated so that the non-plated region is not polluted or damaged, and a plating source is omitted. Furthermore, the sprayed coating can be used for effectively protecting the surface which is in an irregular shape or is uneven as well, and the appearance quality of the surface is not influenced.

The invention discloses red date brandy and a production process thereof. The red date brandy is prepared by using red dates as raw materials through the processes of separating, cleaning, roasting, smoke curing, extruding, soaking, treating with steam, pulping, protecting colors, carrying out ultrasonic enzymolysis, regulating constituents, sterilizing, fermenting, distilling, adsorbing, storing, blending, ageing, and the like, wherein the processes of roasting and smoke curing with peat are used for increasing fragrance and the contents of phenols; the processes of extruding and soaking arebeneficial to extracting date juice; the composite method of treating with steam, protecting the color and carrying out ultrasonic enzymolysis is used for improving the juice yield and the date juicequality; the processes of supplementing a proper quantity of date juice and adding flavor-producing yeast in a later stage mainly aim to generate flavor constituents such as ethyl acetate, isoamyl acetate, diethyl succinate, and the like; the method for distilling for three times is used for collecting flavor constituents of aldehydes, esters, high-grade alcohols, and the like to obtain a high-quality distilled liquor; the adsorbing treatment is used for removing impurities with poor flavors and greatly improving the fragrance and the flavor of the wine; and through storing, blending and ageing, the wine is more mature, fine, smooth and soft. The obtained red date brandy has the advantages of rich and mellow mouthfeel, harmonious red date fruit fragrance, aged oak fragrance, elegant and strong bouquet and pure and mild as well as fine and smooth mouthfeel.

The invention provides a base which can be combined with multiple fruit essences and enable the fruit essences to generate sweetness and aroma. The base contains 2-10% of isoamyl isovalerate, 10-20% of isoamyl acetate, 15-20% of ethyl acetate, 10-20% of butyl acetate, 1-2% of sweet orange oil, 0.8-1.5% of lemon oil, 10-20% of isoamyl butyrate and 20-30% of ethyl butyrate. The base can be used in food, tobaccos and daily necessities, such as toothpaste, shampoo, toilet soap and the like.

An object of the invention is to provide fermented beverages, in particular, beer-taste ones that contain isoamyl acetate abundantly to assure high organoleptic appeal.The invention also provides a process for producing a fermented beverage by performing alcoholic fermentation of an aqueous liquid mixture comprising a feed containing a carbon source and optionally hop, as well as water, wherein a maize protein digest is incorporated as a nitrogen source in the feed either before or during alcoholic fermentation.

The invention discloses natural banana essence. The natural banana essence is characterized by comprising the following components in weight percentages: 12-15% of isoamyl acetate, 6-9% of isoamyl butyrate, 0.10-0.15% of benzyl propionate, 0.01-0.02% of methyl heptine carbonate, 0.035-0.05% of gamma-decalactone, 0.05-0.08% of isobutyric acid cinnamyl ester, 0.1-0.15% of hexenyl acetate, 0.03-0.05% of isoamyl hexanoate, 0.5-1% of banana concentrate, 0.01-0.03% of acetal and 80.385-73.19% of propylene glycol. The natural banana essence overcomes a shortcoming of unnatural and impure flavor of the traditional banana essence formulation, and the banana essence also has more natural flavor by adding raw materials such as the banana concentrate, the gamma-decalactone, the methyl heptine carbonate and the like.

The invention relates to an essence, in particular to a flavored essence for increasing cigarette sour and sweet note. The flavored essence is made of raw materials including, in weight percent, 0.5-0.8% of 1% hydroxycitronellal, 1-2% of ethyl acetate, 5-8% of isoamyl acetate, 0.01-0.5% of beta-damascenone, 0.01-0.8% of dihydroactinidiolide, 0.01-0.8% of alpha-ionone, 1-2.5% of vanillin, 0.5-4% of butyric acid butylester, 0.5-5% of acetic acid, 0.5-5% of lactic acid, 4-10% of dried apricot slices extract, 4-10% of plum extract, 1-5% of tomato extract, 0.5-2% of iris extract, 3-8% of Zimbabwe tobacco extract, 20-30% of propanediol and 4.1-61.67% of 70% ethyl alcohol. The flavored essence solves the problems that an existing cigarette is single in fragrance and obvious in offensive odor and stimulation, smoking satisfaction and enjoyment of the cigarette is improved, the aroma amount of the cigarette is increased, obvious sour and sweet note is given for the cigarette, the flavored essence is rich in fragrance, and stimulation is reduced.

The invention discloses a method for preparing a transmission electron microscope sample of a rust layer powder test sample. The method comprises the following steps of: 1) scraping a rust layer from the surface of the test sample, and grinding into powder; 2) adding the rust layer powder into a collodion and isoamyl acetate solution; 3) adding deionized water into a container, dropping the mixed solution on the water to form a film, immediately fishing the mixed solution by using a clean electron microscope grid, and thus forming an organic film on the electron microscope grid; and 4) drying the electron microscope grid to which the rust layer powder is attached, spraying carbon to the surface of the electron microscope grid, and thus obtaining the rust layer sample for transmission electron microscope observation. The sample preparing process is simple, many rust layer powder test samples can be prepared at one time for observation, and the method is very suitable for preparing the transmission electron microscope sample of the rust layer powder test sample. The prepared sample is representative and can really reflect certain characteristics of an analyzed material, and the resolution of images observed in the transmission electron microscope is high.

The invention discloses printing ink. The printing ink is prepared from the following raw materials in parts by weight: 40-50 parts of permanent yellow, 20-30 parts of acrylic resin, 20-30 parts of lignin-modified phenolic resin, 15-20 parts of saturated polyester resin, 35-40 parts of isoamyl acetate, 4-5 parts of starch phosphate, 20-25 parts of butyl alcohol, 10-15 parts of dimethoxy dipropylene glycol, 2-3 parts of energy stone, 2-3 parts of magnet, 4-6 parts of hydrotalcite, 1-1.2 parts of nonyl phenol polyoxyethylene, 1-1.5 parts of polysorbate, 1-2 parts of butyraldehyde oxime, 1-2 parts of copper octoate and 60-65 parts of water. The invention further discloses a preparation method of the printing ink. The printing ink has the advantages of high drying speed, great adhesive force and superior heat resistance.

The invention discloses an additive for improving resisting phase separation performance and a heat value of methanol gasoline. The additive consists of the following raw materials in percentage by weight: 35-45 percent of aliphatic alcohol, 10-20 percent of aliphatic ether, 15-30 percent of aliphatic ester, 5-15 percent of aliphatic acid, 1-5 percent of aliphatic amine and 0.1-3 percent of emulsifier OP, wherein the aliphatic alcohol is one or more of isopropyl alcohol, n-propanol, n-amyl alcohol, isoamyl alcohol and n-butyl alcohol; the aliphatic ether is one or more of ethylene glycol methyl ether, ethylene glycol ethyl ether and ethylene glycol butyl ether; the aliphatic ester is one or more of butyl acetate, n-amyl acetate and isoamyl acetate; the aliphatic acid is oleic acid and / or naphthenic acid; the aliphatic amine is dimethylamine and / or diethylamine; and the emulsifier OP is emulsifier OP-15 and / or emulsifier OP-20. The methanol gasoline in which the additive is added has superior low-temperature resisting phase separation and water resisting phase separation performances and an obviously improved heat value.

The invention relates to a preparation method of moss scanning electron microscope observation material. The method includes: sorting, to be more specific, sorting moss materials from biological crusts, and stripping leaves after cleaning; fixing, to be more specific, fixing the leaves in glutaraldehyde solution, and washing with buffer solution; dehydrating step by step with alcohol with gradient concentration; replacing, to be more specific, placing the leaves in ethanol-isoamyl acetate mixture for replacing, and placing the leaves in isoamyl acetate solution for replacing; critical point drying; loading onto a table. The method has the advantages that the method is simple and practical, and good in effect, the glutaraldehyde solution serving as the fixing solution is good in fixing effect on polysaccharide, glycoprotein, microtubule and the like, tissue cells do not become brittle after fixing, the influence of leaf cell surface tension can be avoided by the critical point drying, and the microstructures of samples are well preserved; reference is provided for the preparation of ideal moss samples, and the technical blank of related fields in China is filled.

The invention provides a method for manufacturing a ceramic plate painting, which comprises the following steps of: 1) cutting a ceramic plate with the thickness of less than 6mm into plates with image size; 2) storing a frame or a photo to be manufactured into a computer, spraying or printing ink on the surface of the ceramic plate through computer spraying equipment or a flat screen printer; and 3) firing the ceramic plate subjected to spraying or printing at the temperature of between 150 and 400 DEG C to obtain a finished product. The ceramic plate painting manufactured by the method has the same visual art effect as the original work or the photo, can resist the corrosion of chemical substances such as strong acid, strong alkali, isoamyl acetate and the like, and is waterproof, moisture-proof, scratch-resistant, non-fading and environmental-friendly.

The present invention belongs to the field of cigarette packing technology. The essence fore aluminum foil paper for packing cigarette consists of Peru extractum, tree moss concrete, tamarind extractum, refined rose oil, tobacco extractive, bourbonal, ethyl acetate, isoamyl acetate, benzyl cinnamate, erthyl maltol, phenethyl alcohol, carvol, 4-hydroxy-2, 5-dimethyl-3(2H)-furaldone, propylene glycol and alcohol in certain proportion. It has rich fragrancy and high quality stability.

The invention relates to an essence with red bean fragrance. The essence with red bean fragrance is composed of: isoamyl acetate with a mass percentage concentration of 1%, ethyl butyrate with a mass percentage concentration of 1%, beta-damascone, beta-ionone with a mass percentage concentration of 1%, 2-methylpyrazine with a mass percentage concentration of 1%, 2, 5-dimethylpyrazine, 2-acetylpyrazine with a mass percentage concentration of 1%, melonal, 4-ketoisophorone, ethyl maltol, thiol, menthyl acetate, menthol with a mass percentage concentration of 1%, vanillin, ethyl vanillin with a mass percentage concentration of 1%, vanillin propylene glycol acetal, 2-methoxy-3-isobutyl pyrazine with a mass percentage concentration of 1%, and a solvent. The raw materials are added into a container in order according to the formula and are stirred evenly to obtain the essence with red bean fragrance. The essence provided by the invention has outstanding characteristic fragrance of red beans, has strong natural feel and harmonious, soft and delicate fragrance, and can be used for perfuming of drinks, pastries and other food.

An alcohol free or low alcohol fermented malt based beverage is disclosed. The malt based beverage has an alcohol content of not more than 1.0 vol. % preferably not more than 0.7 vol. % having an aroma profile close to the one of alcoholic lager beers. The beverage has 7.00-30.00 ppm ethyl acetate and 0.01-0.20 ppm ethyl butyrate. The beverage preferably has the esters 0.05-2.00 ppm isoamyl acetate; 0.01-0.10 ppm ethyl butyrate; and 0.01-0.05 ppm ethyl hexanoate. The beverage preferably has the higher alcohol 5.00-30.00 ppm (iso-)amyl alcohol. The (iso)amyl alcohol is defined as the sum of 3-methyl butanol and 2-methyl butanol.

A clean and cheap liquid fuel and its production are disclosed. The fuel consists of (by weight) light hydrocarbon (C5 / C6) 50-65, methanol 0-15, alcohol 10-25, modified C12 20-25,, mineral chameleon 0-0.006, hydrogen peroxide 0-0.008, ferrocene 0.002-0.006, acetone 0.3-0.7, n-butyl alcohol 0.04-0.08, acetic acid matte 0-0.007, and isoamyl acetate 0-0.08. The fuel is prepared from byproducts of refineries, such as C5, C6 and C12 hydrocarbons, with high thermal value above 8000 kilocalorie / Kg and stable flame. Its advantages include simple production and use, no odd smell, easy and complete combustion, explosion-resistance, cheap, safety and stability, no environmental pollution and harmless to human beings.

The invention discloses pear essence. The pear essence mainly comprises the following components: 95-97.5% of ethyl alcohol, 0.5-1% of ethyl butyrate, 0.2-0.7% of ethyl acetate, 0.2-0.7% of ethyl propionate, 0.1-0.5% of ethyl-2-methyl butyrate, 0.1-0.5% of butyl acetate, 0.1-0.6% of isoamyl acetate, 0.1-0.5% of ethyl hexanoate, 0.1-0.5% of hexenyl acetate and 0.1-0.7% of methyl 3-methylthiopropionate. Accordingly, the invention also discloses a preparation method of the pear essence. The preparation method of the pear essence comprises the steps of sequentially adding main ingredients of the pear essence in proportion, uniformly mixing and ripening, thus the pear essence product is obtained. By adopting the preparation method of the pear essence, the pear essence has strong fruit juice taste and sufficient characteristic fragrance and can reserve natural flavour of natural pear juice; the pear essence belongs to a beverage product, and reality, smoothness and fragrance of beverage taste can be obviously enhanced.

The invention relates to a favoring pear essence which consists of the following components in parts by weight: 0.1-100 parts of incense, 0-99.9 parts of solvent, 0-20 parts of surfactant, 0-20 parts of cosurfactant and 0-99.9 parts of carrier, wherein the incense consists of the following components in parts by weight: 5-20 parts of hexenyl acetate, 10-35 parts of ethyl butyrate, 6-15 parts of ethyl caproate, 10-30 parts of beta-carypohyllene, 0.5-5 parts of hexdecnoic cid, 3-20 parts of ethyl acetate, 6-15 parts of isoamyl acetate, 4-15 parts of hexyl acetate and 8-25 parts of 3-methylthiopropionate. The favoring pear essence formula disclosed by the invention has fresh pear flavor; and beverage in which the favoring pear essence is added has strong juice feeling as well as flavor and mouthfeel of the fresh pear.

The invention discloses a water-soluble banana essence and a preparation method thereof, wherein the water-soluble banana essence contains the following raw materials by weight: 40-50 parts of ethyl butyrate, 15-30 parts of pentyl butancate, 5-9 parts of clove oil, 150-200 parts of ethanol, 0.2-0.6 parts of vanilla extract, 3-8 parts of ethyl acetate, 8-15 parts of butyl acetate, 8-15 parts of sweet orange oil, 20-30 parts of glycerin, 10-20 parts of isoamyl acetate, 0.5-1 part of vanillin, 0.5-1 part of orange leaf oil, 0.2-0.5 parts of leaf alcohol, 0.2-0.6 parts of geranyl formate, and 50-60 parts of distilled water. The preparation method comprises: uniformly mixing each component, placing the mixture into a glass container, sealing the glass container at 20-30 DEG C for 8-10 days. The water-soluble banana essence provided by the invention can substantially raise freshness feeling of a beverage, can cover bad smell, simultaneously increases banana fragrance, and is easier to drink.

A flavour composition comprises at least 0.5% by weight of one or more of the following group A materials: cinnamic aldehyde, basil oil, tarragon, cis-3-hexenyl acetate, cis-3hexenol, orange oil, lime, citral, and damascone; and at least 3% by weight of one or more of the following group B materials: anethole synthetic, alcohol C10, eucalyptol, methyl salicylate, clove bud oil, carvone laevo, benzyl benzoate, thymol, benzaldehyde, benzyl formate, ethyl salicylate, eucalyptus oil, ionone alpha, iso amyl acetate, rosemary oil, cardamom oil, ginger, eugenol, camomile oil, spearmint, and peppermint. These materials have been identified as being capable of inhibiting the growth of Porphyromonas gingivalis or the protease (arg-gingipain) activity of Porphyromonas gingivalis, and so to possess hitherto unappreciated antimicrobial properties. The invention thus enables compositions to be defined comprising flavour materials that enhance the antimicrobial efficacy of known antimicrobial agents against micro-organisms or metabolic processes associated with gum diseases. The invention also provides a consumer product, particularly an oral or dental care product, including such a flavour composition; a method for reducing or preventing gum disease; and use of the flavour compositions for reducing or preventing gum disease.

The invention relates to wine yeast with low yield of higher alcohol. The wine yeast is characterized in that a certain amount of isoamyl chloroacetate is added in a flat plate for cultivating yeast, the isoamyl chloroacetate generates chloroacetic acid under the catalysis of saccharomycetes isoamyl acetate hydrolase; the chloroacetic acid has a strong restraining effect on the growth of saccharomycetes, if the activity of the ester decomposition enzyme of a mutant strain is high (the higher the activity of the ester decomposition enzyme is, the more higher alcohol produced by the strain is), more chloroacetic acid is produced by decomposition, so that a bacterial colony grows slowly under the inhibition effect of the chloroacetic acid. The wine yeast can be utilized to accurately, conveniently and quickly screen out saccharomycetes with low yield of the higher alcohol. When the saccharomycetes with low yield of the higher alcohol, which is bred through the wine yeast, is used for producing grape wine, the content of the higher alcohol in the grape wine can be lowered by about 10% to 15%; when the saccharomycetes with low yield of the higher alcohol, which is bred through the wine yeast, is used for alcohol fermentation, the content of the higher alcohol in grape wine can be lowered effectively, and the health-care values of the grape wine can be improved.

The invention belongs to the field of quality evaluation of beer and particularly relates to an evaluation method for the flavor harmony of diluted beer after high-concentration brewing, which is used for researching in view of a hydrogen bond system formed among various flavor substances. The evaluation method comprises the following steps: analyzing main chemical displacement and content of the ten main flavor substances in the beer, such as n-propanol, isobutanol, isoamylol, ethyl acetate, isoamyl acetate, malic acid, acetic acid, lactic acid, citric acid and succinic acid; and establishing the evaluating method for the mouth feel flexibility and the flavor harmony of a diluted beer sample after the high-concentration brewing to evaluate the flavor and the mouth feel of the beer sample under different diluting processes. The evaluation method can be used for directly explaining the good and bad mouth feel of the diluted beer after high-concentration fermentation by detecting physicochemical indexes and can be used for removing errors caused by the fact that the subjectivity of sensory evaluation is strong, so that the flavor harmony of the diluted beer after high-concentration brewing can be explained scientifically and seriously.

The invention discloses a reaction-distillation coupling preparing acetic acid series ester method, which comprises the following steps: using glacial acetic acid and alcohol with lower five carbons (alcohol, optal,alcohol isopropylicum, butanol, isobutanol, amyl alcohol and isoamyl alcohol ) for raw material; adopting modified strong acidic phenylethene series basic ion exchange resin for accelerant; preparing by reaction-distillation coupling; getting acetic acid ethyl ester, propyl acetate, isopropyl acetate, butyl acetate, isobutyl acetate, amyl acetate and iso-amyl acetate; making glacial acetic acid and alcohol with lower five carbons to carry on esterification reaction by solid acid catalysis in trickle bed reactor; adding reaction product (acetic acid ethyl ester, propyl acetate, isopropyl acetate, butyl acetate, isobutyl acetate, amyl acetate and iso-amyl acetate), water and cycle still liquor in fractionating tower; getting acetic acid ethyl ester, propyl acetate, isopropyl acetate, butyl acetate, isobutyl acetate, amyl acetate and iso-amyl acetate by separating at the top of the tower. The conversion rate of alcohol with lower five carbons is more than 96%, the recovery ratio of acetic acid ethyl ester (propyl acetate, isopropyl acetate, butyl acetate, isobutyl acetate, amyl acetate and iso-amyl acetate) is more than 95%. The invention has high alcohol conversion rate, which simplifies the operation.

The invention discloses mixed type apple essence which is prepared from hexanal, trans-2-hexenal, ethyl butyrate, ethyl-2-methylbutyrate, ethyl isovalerate, isoamyl acetate, isoamyl isovalerate, hexyl acetate, butanol, hexyl alcohol, a leaf alcohol solution with mass percent concentration of 10%, trans-2-hexenol, a 2-methylbutyric acid solution with mass percent concentration of 10%, an acetic acid solution with mass percent concentration of 10% and solvent propylene glycol. The invention further provides a preparation method of the mixed type apple essence. The mixed type apple essence is mixed according to natural apple fragrance, and the obtained apple essence is strong, natural and vivid in fragrance.

The present invention discloses ink. The ink consists of the following raw materials in parts by weight: 20-30 parts of titanium dioxide; 20-30 parts of vinyl chloride-vinyl acetate resin; 20-30 parts of lignin modified phenolic resin; 15-20 parts of saturated polyester resin; 35-40 parts of isoamyl acetate; 4-5 parts of starch phosphate; 20-25 parts of isopropanol; 10-15 parts of dipropylene glycol dimethyl ether; 2-3 parts of energy stone; 2-3 parts of magnet; 4-6 parts of hydrotalcite; 1-1.2 parts of sulfonated ricinoleic acid; 1-1.5 parts of polysorbate; 1-2 parts of butanone oxime; 1-2 parts of iron octylate; and 60-65 parts of water. The present invention also provides a preparation method for printing ink. The ink provided by the present invention has a relatively fast drying speed, good adhesion, and excellent heat resistance.