Moss scanning electron microscope observation material and preparation method thereof

A scanning electron microscope and moss technology, which is applied to the field of moss scanning electron microscope observation materials and its preparation, can solve problems such as collapse, distortion of sample microstructure, and cell shrinkage easily, achieves good fixation, fills technical gaps, and methods Simple and easy effects

Inactive Publication Date: 2015-02-25
刘永英
View PDF4 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a moss scanning electron microscope observation material and a preparation method thereof, the main purpose of which is to overcome the existing cells that are p

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Moss scanning electron microscope observation material and preparation method thereof
  • Moss scanning electron microscope observation material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] The invention discloses a preparation method of a moss plant scanning electron microscope observation material, and those skilled in the art can learn from the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and the relevant personnel can obviously make changes or appropriate changes and combinations to the method and application described herein without departing from the content, spirit and scope of the present invention to realize and Apply the technology of the present invention.

[0021] A preparation method for moss scanning electron microscope observation material, comprising the following steps:

[0022] a, sorting: sorting th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a preparation method of moss scanning electron microscope observation material. The method includes: sorting, to be more specific, sorting moss materials from biological crusts, and stripping leaves after cleaning; fixing, to be more specific, fixing the leaves in glutaraldehyde solution, and washing with buffer solution; dehydrating step by step with alcohol with gradient concentration; replacing, to be more specific, placing the leaves in ethanol-isoamyl acetate mixture for replacing, and placing the leaves in isoamyl acetate solution for replacing; critical point drying; loading onto a table. The method has the advantages that the method is simple and practical, and good in effect, the glutaraldehyde solution serving as the fixing solution is good in fixing effect on polysaccharide, glycoprotein, microtubule and the like, tissue cells do not become brittle after fixing, the influence of leaf cell surface tension can be avoided by the critical point drying, and the microstructures of samples are well preserved; reference is provided for the preparation of ideal moss samples, and the technical blank of related fields in China is filled.

Description

technical field [0001] The invention relates to the field of preparation of scanning electron microscope observation materials, in particular to a moss scanning electron microscope observation material and a preparation method thereof. Background technique [0002] The micromorphological characteristics of moss plants are often an important basis for classification, such as the presence or absence of warts on the surface of capsule teeth, the distribution of warts, the shape of warts, the surface decoration of spores, the presence or absence of warts on the surface of leaf cells, the number and shape of warts, Bud structure, presence or absence of warts on rhizoids, and special accessory structures such as supracostal filaments or pectin in leaves, these characteristics are clearly differentiated among taxa (such as families, genus, and species), and are stable within taxa. Due to its tiny shape, the scanning electron microscope has become an important observation tool. Bio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N1/28G01N23/22
Inventor 刘永英赵建成张含笑牛景彦
Owner 刘永英
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap