Moss scanning electron microscope observation material and preparation method thereof

Abstract

The invention relates to a preparation method of moss scanning electron microscope observation material. The method includes: sorting, to be more specific, sorting moss materials from biological crusts, and stripping leaves after cleaning; fixing, to be more specific, fixing the leaves in glutaraldehyde solution, and washing with buffer solution; dehydrating step by step with alcohol with gradient concentration; replacing, to be more specific, placing the leaves in ethanol-isoamyl acetate mixture for replacing, and placing the leaves in isoamyl acetate solution for replacing; critical point drying; loading onto a table. The method has the advantages that the method is simple and practical, and good in effect, the glutaraldehyde solution serving as the fixing solution is good in fixing effect on polysaccharide, glycoprotein, microtubule and the like, tissue cells do not become brittle after fixing, the influence of leaf cell surface tension can be avoided by the critical point drying, and the microstructures of samples are well preserved; reference is provided for the preparation of ideal moss samples, and the technical blank of related fields in China is filled.

Description

technical field [0001] The invention relates to the field of preparation of scanning electron microscope observation materials, in particular to a moss scanning electron microscope observation material and a preparation method thereof. Background technique [0002] The micromorphological characteristics of moss plants are often an important basis for classification, such as the presence or absence of warts on the surface of capsule teeth, the distribution of warts, the shape of warts, the surface decoration of spores, the presence or absence of warts on the surface of leaf cells, the number and shape of warts, Bud structure, presence or absence of warts on rhizoids, and special accessory structures such as supracostal filaments or pectin in leaves, these characteristics are clearly differentiated among taxa (such as families, genus, and species), and are stable within taxa. Due to its tiny shape, the scanning electron microscope has become an important observation tool. Bio...

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Problems solved by technology

[0004] The invention provides a moss scanning electron microscope observation material and a preparation method thereof, the main purpose of which is to overcome the existing cells that are prone to shrinkage, collapse and other deformations in the process of preparing the moss electron microscope observation material, resulting in severe sample microstructure. distortion defect

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