The invention relates to the technical field of microorganisms and
fermentation engineering, and particularly discloses a
Pichia kluyveri strain H421, and the preservation number of the
Pichia kluyveri strain H421 is CGMCC (China General
Microbiological Culture Collection Center) NO.34649. The
bacterial strain H421, commercial
saccharomyces cerevisiae D254 and
schizosaccharomyces pombe CICC 32484 are sequentially inoculated and fermented, the
aroma of the brewed
lonicera caerulea fruit wine is dominated by
isoamyl acetate and
phenethyl acetate, the brewed
lonicera caerulea fruit wine is rich in various esters such as
ethyl caprylate, ethyl caprate and
diethyl succinate, and the characteristic flower and fruit
aroma is jointly formed. The strain H421 has excellent
alcohol resistance,
acid resistance and SO2 resistance, and can adapt to the
fermentation environment of the
lonicera caerulea fruit wine. By adopting a multi-strain sequential
inoculation fermentation method including the strain, the content of
malic acid in the lonicera caerulea fruit
wine can be remarkably reduced, and the variety and content of
aroma substances are greatly increased, so that the problems of strong acerbity and single aroma of the lonicera caerulea fruit
wine are effectively improved.