Amora-producing Geotrichum candidum strain of high-yield exopolysaccharides, exopolysaccharides and volatile flavor compounds

A technology of exopolysaccharide and Geotrichum candidum, which is applied in the field of microorganisms, can solve the problems of the development and application of natural flavors, and achieve the effect of good texture and flavor, increased variety and content, and high exopolysaccharide content

Active Publication Date: 2012-06-13
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

As humans return to nature and advocate natural food, the demand for safe biological flavors and spices is increasing. Although natural flavors and spices extracted from animals and pl

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  • Amora-producing Geotrichum candidum strain of high-yield exopolysaccharides, exopolysaccharides and volatile flavor compounds
  • Amora-producing Geotrichum candidum strain of high-yield exopolysaccharides, exopolysaccharides and volatile flavor compounds
  • Amora-producing Geotrichum candidum strain of high-yield exopolysaccharides, exopolysaccharides and volatile flavor compounds

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Embodiment Construction

[0025] Below in conjunction with the examples, the present invention is further described, the following examples are illustrative, not limiting, and the protection scope of the present invention cannot be limited by the following examples.

[0026] The experimental methods used in the following implementation examples are conventional methods unless otherwise specified; the materials and reagents used, etc., can be obtained from commercial sources unless otherwise specified.

[0027] Wort culture medium: add 4 times the amount of tap water after drying and pulverizing the malt, put it in a 60°C incubator, saccharify to a sugar content of 12°Bx, and filter to obtain the wort juice medium.

[0028] Bengal red solid medium: peptone 5g, glucose 10g, potassium dihydrogen phosphate 1g, magnesium sulfate heptahydrate 0.5g, agar 15g, 1 / 3000 Bengal red solution 100mL, chloramphenicol 0.1g, distilled water to 1000mL, natural pH .

[0029] YPD medium: yeast extract 10g, peptone 20g, gl...

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Abstract

The invention relates to an amora-producing Geotrichum candidum strain of high-yield exopolysaccharides, the exopolysaccharides and volatile flavor compounds. The strain is named as WGK2-1 and the catalog name of the strain is Geotrichum candidum. By a flat gradient coating separation process, the WGK2-1 strain is screened out from a traditional fermented dairy product. Cow milk is used as a medium to culture the strain at 25-35 DEG C. Therefore, a lot of exopolysaccharides can be generated and can be used as a yogurt starter culture to ferment cow milk. And a lot of volatile flavor compoundscan be generated. The screened strain WGK2-1 of the high-yield exopolysaccharides is an aroma-producing strain, which can generate a plurality of volatile aroma components. The generated volatile flavor compounds are mainly esters and acids and can effectively raise the variety and content of aroma compounds in cow milk, reduce the variety and content of aldehyde and ketone substances and improvethe flavor of fermented milk.

Description

technical field [0001] The invention belongs to the field of microbes, and relates to a newly screened strain, in particular to a Geotrichum candidum strain producing high exopolysaccharide, exopolysaccharide and volatile flavor substances. Background technique [0002] Geotrichum candidum (Geotrichum candidum) is a common fungus, which belongs to the subphylum Deuteromycotina, the family Plexaceae, the family Ovalspora, and the genus Geotrichum. The morphological characteristics of Geotrichum candidum are between yeast and mold, and the reproduction method is mainly fission, with blastospore among a few strains. The growth temperature ranges from 5 to 38°C, the growth pH ranges from 3 to 11, and has wide ecological adaptability. A single Geotrichum candidum has a certain degree of phenotypic variability, different strains within the same species show genetic polymorphism, the colony color ranges from white to cream, a few strains are light brown or dark brown, and the text...

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Application Information

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IPC IPC(8): C12N1/14C12P19/04C12P1/02A23C9/12A23C9/127C12R1/645
Inventor 王昌禄高鑫陈勉华王玉荣李风娟李贞景
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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