54 results about "High amylase" patented technology

The invention discloses a starch type impregnating compound for electronic grade spun yarns. The impregnating compound is prepared from the following components in percentage by weight: 1.5-4.5 percent of etherified high amylase corn starch with amylose content of 55-70 percent, 1.0-4.0 percent of etherified low amylase corn starch with amylose content of 27-30 percent, 0.10-2.00 percent of hydrogenated cottonseed oil, 0.05-0.80 percent of emulsifier, 0.10-2.00 percent of lubricant, 0.001-0.005 percent of antibacterial agent, and the balance of deionized water. The electronic spun yarns produced by the impregnating compound has the advantages of high operation rate, high yarn breaking strength, proper hardness, low hairiness rate of bobbin yarns, few broken ends in the warping process and the like.

The invention discloses a polyvinyl alcohol / high amylose biodegradable material and a melting preparation method thereof. The method comprises the following steps: uniformly mixing polyvinyl alcohol, high amylase, heat stabilizer, inorganic filler, low molecular weight plasticizer and processing assistant agent in a high-speed mixer at the temperature of 70 to 120 DEG C; and then, melting, extruding and pelleting the mixture in a double-screw extruder, wherein the temperature of a charging barrel is 130 to 190 DEG C. By the method, a composite system of the low molecular weight plasticizer is rationally selected and a proper amount of processing assistant agent and heat stabilizer are added to successfully implement the thermoplastic molding of the polyvinyl alcohol / high amylose, therefore, not only the melting processing problem of the polyvinyl alcohol / high amylase is solved, but also the processability, mechanical property and biodegradability of the composite material are improved.

The invention discloses a method of reducing the content of amylase in hybrid rice and belongs to the technical field of rice genetic breeding. In the method, an original hybrid rice combination: one of a parent of a non-glutinous sterile line / a non-glutinous restorer is modified into a glutinous parent. The hybrid rice is prepared through a hybridization method of a glutinous sterile line / non-glutinous restorer or a non-glutinous sterile line / a glutinous restorer to obtain a hybrid rice variety in which the content of the amylase is lower than that of the original hybrid rice. In the invention, by means of a genetic effect and a genetic dosage effect of a glutinous gene wx, a near-isogenic line combination is reduced in the content of the amylase. In addition, a growth period, a plant and leaf form, panicle traits, floral organ traits, yield traits, disease resistant performance, adaptability and the like of the near-isogenic line combination are free of significant different when the near-isogenic line combination is compared with the original hybrid rice combination. Through quality analysis and taste evaluation, a new hybrid rice variety being appropriate in the content of the amylase and having the taste and the quality which are suitable for people in different region, thereby achieving an object of genetically improving a poor taste and a poor quality due to a high amylase content in the hybrid rice in the prior art.

The invention provides a high-efficiency flavor-enhancing strain and application thereof in improving the tobacco quality. The high-efficiency flavor-enhancing strain is Bacillus sp. J3 with the preservation number of CCTCC NO: M 2018707. The strain is first separated from the surface of Zimbabwe flue-cured tobacco leaves and found to have high amylase, catalase and protease activities. The wholegenome sequencing finds that the strain contains multiple sets of oxidative stress systems, high activity amylase expression systems and multiple protease gene information. In addition, the strain also has a polyglutamic acid synthesis gene cluster, and can synthesize polyglutamic acid in a large amount extracellularly. On one hand, the Bacillus sp. can shorten the time of artificially aging of the flue-cured tobacco and improve the aroma of the flue-cured tobacco and smoking quality; on the other hand, the strain can be used as a fertilizer synergist for tobacco planting to improve the planting quality of the tobacco from the tobacco planting.

The invention discloses an oxidation-resistant amylase mutant and a preparation method thereof, and belongs to the field of genetic engineering. The preparation method of the invention adopts Bacillus alcalophilus JN21 (CCTCC NO: M 2011231) amylase as a female parent, and performs site-directed mutagenesis of the Bacillus alcalophilus amylase sequence by molecular biology technology. Under the reconstruction conditions, the Bacillus alcalophilus amylase is treated at 40 DEG C in 500 mM H2O2 environment for 30 min, and the enzymatic activity residue is increased from 18% of a control (before mutation) case to 92%; with the strategy, the oxidation resistance of the amylase is greatly increased, which lays a foundation for the application of the amylase in industrial production fields of weaving desizing, washing agent additives, and the like. The strategy has important guidance meaning for the reconstruction of oxidation resistance of amylase and other enzymes.

The invention discloses a higher-heat-stability amylase mutant, and a preparation method and application thereof, belonging to the field of enzyme engineering. A molecular biology technique is utilized to perform multiple site-directed mutagenesis to obtain the higher-heat-stability amylase mutant. The half life of the obtained amylase at 60 DEG C is enhanced to 23.9 minutes from 3.2 minutes of the control sample (before mutation). The scheme can obviously enhance the heat stability of the amylase, and provides basis for industrial production. The scheme has important instruction meanings for property modification of other enzymes.

The invention discloses a method for modifying corn starch with different amylose contents by air atmospheric pressure plasma jet. The method comprises preparing waxy corn starch, common corn starch and high amylase corn starch into starch emulsions having a mass concentration of 5-15%, directly treating the starch emulsions with an air atmospheric pressure plasma jet device for 1-7 minutes, centrifuging the starch emulsions to obtain precipitates, washing the precipitates for 3 times with absolute ethanol, and performing vacuum freeze drying to obtain modified starch. The method uses the airatmospheric pressure plasma jet technology to treat the three corn starch emulsions, and introduces some oxygen-containing polar groups into the corn starch for hydrophilicity improvement, mainly reflected in increase of water bonding ability and water expansion volumes, of the starch so as to further improve the practical application performance of the three kinds of corn starch. The method can also provide a new direction for starch modification for scholars who study the direction in the future, and provides more theoretical supports for the starch research field. The modified starch produced by the method can also be better applied to convenience food.

A liquid cleaning agent containing: a) 20 to 70 wt % water, b) at least one amylase preparation, c) at least one Ca2+-ion source, d) lactic acid or a lactic acid salt, is notable for elevated amylase stability and improved cleaning performance.

The invention discloses high amylase corn starch paste and a preparation method thereof. The method comprises the following steps that (1) high amylase corn starch is added into water to obtain high amylase corn starch water suspension; a sodium hydroxide water solution is added into the high amylase corn starch water suspension to be uniformly stirred to obtain a mixed solution; (2) the mixed solution is stirred; then the pH value of the stirred mixed solution is regulated to be neutral, and the high amylase corn starch paste is obtained; and (3) the high amylase corn starch paste is subjected to high-pressure homogenizing processing. The preparation method provided by the invention is simple and reliable, is convenient to operate and does not pollute environment, and the pasting cost of the high amylase corn starch is greatly saved. The high amylase corn starch paste has good liquidity, high transparency, uniform paste structure and no residual starch granules, and the starch paste viscosity of high amylase corn can be regulated through the selection of homogeneous pressure.

The invention discloses an oxidation resistive amylase mutant as well as a preparation method and application thereof, and belongs to the field of genetic engineering. According to the invention, the bacillus alcalophilus amylase sequence is mutated in directed site by using molecular biological technique based on bacillus alcalophilus JN21 (CCTCC NO:M2011231) amylase as a female parent. In the modifying condition, the bacillus alcalophilus amylas is treated for 5 hours at 40 DEG C in 500mM H2O2, and enzymatic activity residue is changed from 10% (before mutation) to 72%. The oxidation resistance of amylase can be greatly improved through the policy so as to lay a foundation in the industrial production fields such as spinning desizing and detergent and additive. The policy has an import guiding meaning for modifying the oxidation resistance of amylase and other enzymes.

The invention provides a high-specific-activity amylase mutant with good ability to degrade raw starch and a preparation method and application thereof, and relates to the field of gene engineering. Acid amylase derived from Talaromyces leycettanus JCM12802 is used as a female parent, and the wild type is subjected to site-directed mutagenesis by means of molecular biological techniques. Under the modification condition, the amylase mutant has significantly higher specific activity than the wild (before mutation) female parent. By using the scheme, it is possible to greatly increase the specific activity and acting conditions of amylase, and basis is provided for the amylase in industrial production field. The scheme is of important guidance for increasing the catalytic efficiency and specific activity of amylase and other enzymes.

The invention discloses a sorghum and passion fruit raw material brewing method. The method comprises the following steps: (1) preparing raw materials; (2) pretreating grain; (3) matching the raw materials; (4) performing enzymolysis; (5) mixing with yeast, adding yeast, and fermenting; (6) adding passion fruit pulp and fermenting; (7) sealing and post-fermenting; (8) centrifuging, squeezing, filtering and clarifying; (9) blending; (10) sterilizing and cooling; (11) filling and sealing; and (12) storing and ageing. According to the invention, grains and fruit raw materials are fully utilized, traditional Qu liquor, fruit liquor, modern raw material wine brewing and enzyme preparation wine brewing methods are perfectly combined, the problem that fruits are difficult to sell can be solved, the problem of grain supply guarantee is considered, the energy consumption of wine brewing is reduced, the enzymolysis reaction degree of amylase for converting raw starch into glucose is improved, the conversion rate of converting starch into glucose is jointly improved, the enzymolysis saccharification conversion rate is improved, and the wine yield is improved.