184results about How to "Reduce screening costs" patented technology

An indirect solid free form scaffold manufacturing technique is provided. More particularly, the present invention provides a set of molds, casting methods, mold removals, and surface modification techniques that are compatible with image-based design methods and with solvent, melt, and slurry casting of polymers and ceramics.

The invention relates to a method for screening drug target genes based on a CRISPR / Cas9 high-throughput technology. The method comprises the steps of firstly establishing a sgRNA library; secondly packaging the sgRNA library by using slow viruses, and collecting the viruses; thirdly screening the sgRNA library in a cancer cell line; fourthly extracting cells obtained by screening and genome DNA of the cells before screening; and finally enriching sgRNA in genome DNA. Compared with the prior art, the method has the advantages that a CRISPR / Cas cell screening process is improved, the virus infection efficiency is determined with a simple and convenient method by utilizing puromycin resistance of infected cells, and MOI values of the viruses are determined; more importantly, a virus packaging method is greatly optimized, so that the virus packaging efficiency is improved to be more than 5 times that of a conventional method, and large-scale drug target screening cost can be greatly reduced; and the method is used for promoting industrialization of cancer drug target screening.

The invention discloses a flexible multifunctional sensor, a preparation method and an application thereof. The flexible multifunctional sensor comprises electrode layers and a flexible composite material layer, wherein two electrode layers exist and are respectively arranged at two sides of the flexible composite material layer and cover the flexible composite material layer. According to the flexible multifunctional sensor, the physical property of a thermoelectric material is used for realizing thermoelectric power generation and realizing temperature sensing. Furthermore the composite material with an elastic net structure can realize resistive pressure sensing. Based on a voltage signal and a current signal, real-time monitoring for pressure and temperature can be realized simultaneously. When a thermoelectric material composite system bears the function of a temperature field and a pressure field, a voltage signal and a current signal can be simultaneously generated. If the generated voltage is large enough, the flexible multifunctional sensor can be used as a self-powered multifunctional sensor. Through patterning an integrated matrix, selection between voltage storage and multifunctional sensing can be realized.

The invention provides a stepwise utilization method of decommissioned batteries. The stepwise utilization method comprises decommissioned battery sorting, decommissioned battery grouping and formingan energy storage system by the decommissioned batteries. The invention further provides a stepwise utilization system of decommissioned batteries, comprising an energy storage system and an energy management unit for controlling the energy storage system to perform charging and discharging, the energy storage system is composed of a plurality of battery clusters with a working voltage of 570 to 832.2V, and each battery cluster comprises a plurality of battery packs connected in parallel; and each battery pack comprises decommissioned batteries connected in series, a PCS and a monitoring unit.According to the stepwise utilization method and system provided by the invention, the consistency problem of the decommissioned batteries is solved through string distributed architecture, which greatly simplifies the process, can not only refine the management of energy storage batteries, but also can ensure the battery consistency to the greatest extent. In addition, the long-term stable operation of the entire system can be ensured, and the safety and the service life of the energy storage system are also objectively improved; and meanwhile, the stepwise utilization cost is reduced by thestepwise utilization method and system provided by the invention, so that the stepwise utilization method and system provided by the invention can be widely used, promoted and applied.

The invention discloses a screening method for multi-target drugs and / or pharmaceutical combinations, belonging to the field of biomedicine technology. According to the invention, the screening method comprises the steps of: (1) searching a drug target database, summarizing drug targets, understudied targets and drugs corresponding to the targets to obtain data of corresponding relations between the targets and the drugs; (2) screening association target-target combinations through a systemic genetics method; and (3) according to the data of corresponding relations between the targets and the drugs obtained in the step (1) and the association target-target combinations obtained in the step (2), screening multi-target drugs and / or pharmaceutical combinations. According to the invention, the screening method is low in cost and high in efficiency, and has a broad applying prospect in the field of drug re-positioning, development and design.

The invention discloses a docosahexaenoic acid-producing bacterium and a mutation and screening method and application thereof. The strain is classified and named as Crypthecodinium cohnii HX-308, is preserved in China Center for Type Culture Collection, and has the preservation number M208246. The strain is a DHA-producing bacterium with high yield, has the DHA content 1.53 times of a starting strain, stable physiological-biochemical characteristic and stable heritability, and simultaneously can produce polyunsaturated fatty acid through liquid culture and fermentation; the content of unsaturated fatty acid reaches 20.4g / L, wherein the DHA content reaches 8.16g / L; and the strain is a good strain for producing natural DHA, and has excellent industrial production prospect.

The invention relates to a delayed ignition device, especially a delayed ignition control device and an electronic detonator for timing initiation. For the problems of the prior art, a delayed ignition control device and an electronic detonator are provided; upon reaching the timing initiation time of the electronic detonator, an energy-storage capacitor can discharge electricity to a detonating fuse head by matching a processor and a switching tube; the component improves the use security of the electronic detonator; meanwhile, in order to prevent the electrostatic influence produced by putting the device into an electronic detonator, an electrostatic protection structure is formed on an electronic detonator cap wire bonding pad accessory in front of a current-limiting resistor. The device comprises a cap wire M and a cap wire N, and comprises an electrostatic protection device, an electronic delay module, an energy-storage device, and a detonating fuse head which are integrated on a printed board. The electrostatic protection device is configured to release static electricity input at any two of the cap wire M, the cap wire N and a detonator shell. The electrostatic protection structure is formed on the cap wire input end bonding pad. The electronic delay module is in both-way communication with an exploder via the cap wire M and the cap wire N.

The invention discloses a breast ultrasonic screening method which comprises the following steps: acquiring a depth image of a chest area of a user; performing model reconstruction according to the depth image so as to obtain a three-dimensional structure model of an area to be scanned, and generating a scanning track of an ultrasonic probe according to the three-dimensional structure model; generating a motion control code according to the scanning track, and inputting the motion control code into a scanning mechanism to control the scanning mechanism to drive the ultrasonic probe to conductultrasonic scanning on the breast area of the user; analyzing and processing the obtained ultrasonic image, and generating diagnosis results. With the help of automation technology and artificial intelligence technology, the breast ultrasonic screening method disclosed by the invention can make mass screening of breast cancer with low cost in a large scale possible, greatly increases the proportion of Chinese women of right ages to participate in breast cancer screening and contributes to prevention and control of breast cancer.

The invention relates to an in-vitro directed coevolution method for modifying L-phenylalanine gene engineering strains, which is realized in a way that: using genes aroG and pheA on an L-phenylalanine gene engineering strain constructed in the room as a whole; carrying out the in-vitro directed coevolution modification by using an error-prone PCR technology and a recombinant DNA technology; and screening to obtain a mutant strain, of which the yield of L-phenylalanine is increased by 114%. In the invention, by modifying the gene formed by coupling and connecting aroG and pheA in series, the key genes aroG and pheA in the metaboly process of the L-phenylalanine are used as a whole to carry out the directed modification, thereby obtaining a new metabolic balance, so that the expressed enzyme has high-efficiency catalytic activity and can resist the feedback inhibition of the L-phenylalanine, thereby obtaining the new modified high-yield L-phenylalanine gene engineering strain by screening. The method can provide an example for modifying the acid production rate of any amino acid gene engineering strain.

The present invention relates to mixed bacteria producing a biological surfactant, and a screening method thereof, wherein the mixed bacteria is formed by mixing three strains, and the three strains respectively are Pseudomonas stutzeri having the preservation number of CCTCC AB 205091, Nocardioides ginsengagri having the preservation number of CCTCC S2013441, and Bacillus licheniformis having the preservation number of CCTCC AB 205141. According to the present invention, oil extraction strains to be screened are selected, the selected oil extraction strains to be screened are activated and cultured, the activated and cultured oil extraction strains to be screened are subjected to primary screening by using a blood flat plate method to obtain a primary screening fermentation broth, and the primary screening fermentation broth is re-screened by using an oil ring removing method so as to obtain the mixed bacteria; and the mixed bacteria integrates the advantages of the three stains and can produce the biological surfactant capable of being applied in the oilfield oil extraction, and the screening method has characteristics of wide application range, effective screening cost reducing, high accuracy, strong selectivity, simple process, easy operation, and short period compared with the prior art.

The invention provides a targeted antibacterial peptide against Gram-negative bacteria and a preparation method and an application. A sequence of the targeted antibacterial peptide is as shown in a sequence table SEQ ID No. 1, and in the preparation method, an R language is utilized for statistical analysis of amino acid sequence characteristics of a natural antimicrobial peptide library, variouspeptide chain parameters affecting antibacterial activity of a peptide chain having the antibacterial activity only against Gram-negative bacteria are statistically analyzed, and the optimal amino acid composition is obtained by screening, namely K, G, L, with the number of positive charges being 4, and the hydrophobicity being 30%-50%. Tryptophan is inserted into the center of a screening sequence to obtain a centrally symmetric short peptide sequence through design, so as to obtain a short-chain narrow-spectrum antibacterial peptide sequence which is low in toxicity and highly effective, andnot easy to cause disorder of immune regulation in a body. The targeted antibacterial peptide is beneficial to maintaining micro-ecological balance while killing harmful bacteria. The antibacterial peptide has relatively high activity, high stability, relatively low cytotoxicity, and substitute antibiotics, thereby having an application potential for becoming a safer and environmentally-friendlynovel antibacterial product.

The invention discloses a primer pair for screening mammalian experiment animal SNP (Single Nucleotide Polymorphism) markers and application thereof. 30 pairs of primer pairs are provided, and sequences are represented from SEQ ID NO:1 to SEQ ID NO:60; each primer pair can be used for amplifying genomic sequences of experiment animals including dogs, pigs, rats, mice, rhesus monkeys and rabbits under the same condition; and sequences are represented from SEQ ID NO:61 to SEQ ID NO:240. Sequence species amplified by the same pair of primers have difference. The application of the primer pair can greatly reduce the screening cost of the mammalian experiment animal genetic markers and simplify the operation, so that the standardization of experiment animal genetic quality monitoring is facilitated.

The invention discloses an adhesion substrate for artificial cultivation of porphyra yezoensis conchocelis. The adhesion substrate comprises a cement base, oyster shells, and auxiliary handles; the cement base main body is made of cement; the oyster shells are closely connected and distributed on the adhesion substrate; a surface with a pearl layer of each oyster shell faces upwards; the rough surface of each shell is embedded into the cement base; the auxiliary handle is embedded into the cement base; and two ends of the cement base are respectively provided with one auxiliary handle, and themain function is to assist the movement and distribution of the adhesion substrate. A mode of integration and fixing of the shells is adopted in the application, the size and specification limitationof raw materials of adhesion substrates is broken through, and the utilization rate of the shells is improved. The adhesion substrate with the cement base can be cleaned with the match of high-pressure water-flow washing, meanwhile, displacement of the adhesion substrate is avoided, and the cleaning efficiency is improved, so that the labor cost is reduced. The utilization area of a single adhesion substrate is effectively increased; and compared with the area of conventional adhesion substrates, the area is increased by 15-30 times, so that the breeding efficiency is remarkably improved, andthe production management cost is reduced.

The invention relates to a multistage screening device for chemical powder materials. The multistage screening device comprises a bottom plate, a screening barrel, a connecting frame, a shaking component, a connecting component and a screening component. According to the screening component, multi-stage vibration screening treatment can be conducted on chemical powder materials, the chemical powder materials adhering to the inner wall of a screening shell can be brushed off in time through a cleaning unit, waste of the chemical powder materials is effectively reduced, the screening cost is reduced, and secondly, scraping teeth arranged on the cleaning unit not only can play a role in stirring chemical powder materials in the screening shell in the screening process, but also can crush the caked chemical powder materials to prevent the caked chemical powder materials from blocking screening holes, and meanwhile, in the screening process, the shaking component and the connecting component can be matched with each other, so that the chemical powder materials in the screening shell are driven to shake, and the screening efficiency of the chemical powder materials is effectively improved.

The invention discloses a primer sequence and a test kit for gene detection of safe medication for children, belonging to the technical field of molecular diagnosis. The test kit comprises 22 pairs ofamplification primers and can specifically amplify 22 common locus regions of the safe medication gene for children; the 22 single-base extension primers are used for detecting genotypes of 22 loci of the safe medication gene for children; in addition, the test kit comprises a special reagent for pretreatment and detection. The test kit provided by the invention can realize one-hole detection ofdifferent genotypes of 22 common loci related to clinical safe medication genes for the children, and has the advantages of high sensitivity, strong specificity, high accuracy, simple and convenient operation, low cost, high throughput, rapid detection, automatic result interpretation and easy clinical popularization and application. The test kit provided by the invention can be applied to gene detection of safe medication for the children, provides a reliable detection system and test kit for safe medication for the children, and has important clinical application value and good market application prospect.

The invention discloses a kit for detecting a novel-coronavirus-susceptive gene and a detection platform. The kit comprises primers shown in SEQ ID NO: 1 to SEQ ID NO: 38. The kit disclosed by the invention is high in sensitivity and high in specificity, human gDNA nucleic acid samples (about 5-6 gene copies) to the minimum of 0.01ng / microliter can be detected, and the consumption of samples can be lowered; 2-hole detection can be achieved through 35 loci, and the accuracy reaches 100%; through a repeated-optimized-completed pretreatment reagent, a variety of ingredients can be premixed, the difficulty of system preparing and operating by detection personal during clinical application is simplified greatly, operations are facilitated, and the threshold of use and the difficulty of beginning are effectively lowered; and through being matched with the integrated detection platform, the operation is simple, convenient and rapid, and results are detected and analyzed automatically.

The invention relates to a method and a kit for detecting high-risk HPV (human papillomavirus) E6 / E7 mRNA (messenger ribonucleic acid) by ligase. The method is characterized in that specific connection primers are connected into a cDNA (complementary deoxyribonucleic acid) fragment complementary with a target sequence of the HPV E6 / E7 mRNA under the action of the ligase, and the successfully connected cDNA fragment can be amplified by a pair of universal primers in a later fluorescent PCR (polymerase chain reaction) system, in other words, eighteen high-risk HPVs capable of causing cervical cancer can be detected in the same amplification system by the single fluorescent PCR technology, so that the problems, such as low amplification efficiency and sensitiveness, caused by excessive primers are solved effectively. The aim of HPV E6 / E7 mRNA detection is to screen out high-risk groups in HPV-infected people groups, so that the method has more significance in clinical diagnosis as compared with existing HPV genome L1 distinguishing type detection methods.

The invention discloses a primer combination sequence and kit for detecting child safe medication-related gene mutation sites. The kit includes 23 pairs of amplification primers and 23 single-base extension primers; the 23 pairs of amplification primers can specifically amplify the 23 common mutation site regions of child safe medication-related genes, and the 23 single-base extension primers areused to detect the 23 mutation site genotypes of the child safe medication-related genes; and the kit further includes special reagents for pretreatment and detection. The kit provided by the invention can realize one-hole detection of the 23 mutations related to the clinical child safe medication-related genes, has high sensitivity, strong specificity and high accuracy, and is simple to operate,low in cost and high in throughput, the detection is fast, automatic interpretation of results is realized, and the clinical promotion and application are easy to realize; and the kit can be applied to the detection of the genes related to safe medication of newborns or children, guide clinical correct medication, avoid medication risks, reduce medication injury, and truly achieve precise medication.

The invention relates to an efficient arsenic-absorbed ecological ciliate desert grass screening method. The method comprises the following steps: conducting field ciliate desert-grass acquisition in multiple regions of China, and carrying out captive breeding of ciliate desert-grass seedlings; conducting short-term water culture experiment in arsenic-containing nutrient solution by utilizing ciliate desert-grass seedling of the right age in multiple regions, and initially screening out 2-5 ecological ciliate desert-grass with higher arsenic concentration of the overground part; and transplanting the 2-5 initially screened out ecological ciliate desert-grass with higher arsenic concentration of the overground parts to different gradients of artificial simulated arsenic-polluted soil for culturing for 12 weeks, and preferentially screening out efficient arsenic-absorbed ecological ciliate desert grass by taking overground part biomass liveweight, overground part arsenic concentration, overground part arsenic absorption total quantity, and arsenic tolerance index number of the ciliate desert-grass as indexes. The screening method is simple and feasible, high in routinization, and easy to popularize, and has important meanings on restoring the arsenic-polluted soil and protecting the quality safety of agricultural products.

The invention discloses a rapid screening and grading method of retired battery modules. The method includes the following steps of screening the battery modules according to the appearance, conducting charging and discharging testing on the battery modules, grading the qualified batteries in discharging testing, and recombining the graded battery modules according to the combining requirements for cascade utilization. Only by means of the appearance detecting and capacity testing project, the aim of screening and grading the retired battery modules is realized, the screening speed is increased, the screening cost is reduced, and the rapid screening aim is realized. By grading the screened battery modules, it is shown that the higher the grade of one module is, the better the quality of the battery module is. During the cascade utilization for an energy storage system, the battery modules of the same grade are connected in series for utilization, the buckets effect is easily avoided, and the capacity of the battery modules is brought into better play.

The invention relates to Saccharomyces cerevisiae and a preparation method thereof. A preparation method of high-nitrogen fresh yeast is mainly characterized by comprising the following steps of: (1) preparing a strain, i.e., Saccharomyces cerevisiae which is collected in the China General Microbiological Culture Collection Center with the collection number of CGMCC No.5004; (2) preparing a culture medium in a certain mass ratio; (3) pretreating sweet sorghum juice; (4) fermenting; (5) undergoing an adaptive phase at the ventilation quantity of 0.8-1.2 V.V.m and the stirring speed of 300-500 r / min for 4-6 hours, and adding nutritional salts, including 0.2-3 percent of (NH4)2SO4, 0.03-1.5 percent of MgSO4 and 0.2-1.5 percent of KH2PO4; (6) undergoing an exponential growth phase for 10-20 hours, fermenting at the fermenting temperature of 32-35 DEG C, undergoing a decline phase at the ventilation quantity of 0.3-0.5 V.V.m and the stirring speed of 80-150 r / min, stopping feeding liquid glucose, measuring the concentration of residual sugar in fermented cheese liquid, and stopping fermenting if the concentration of residual sugar is less than 0.5 percent; and (7) treating a fermentation liquid. In the method, the sweet sorghum juice is taken as a carbon source in the fermentation liquid. Compared with other carbon source culture mediums such as honey and beet, the sweet sorghum juice has the advantages of simple pretreating process, saving in energy consumption and increase in production efficiency.

The invention relates to a pecan plumpness screening device and belongs to the field of machinery special for food production. The pecan plumpness screening device comprises a motor and a flotation tank, wherein the flotation tank comprises a material inlet, material outlets and at least two partition plates mutually separated with a certain distance, and an output end of the motor is connected with a material inlet end of the flotation tank in matching mode so as to form water flow flowing from the material inlet to the material outlets. The partition plates are arranged among the material outlets to form different separation areas. According to the theory of buoyancy of water, the pecan plumpness screening device can achieve the ideal effect of classifying and screening pecans, replaces manual pecan screening with automatic screening, saves labor expenditure, reduces screening cost, and is accurate in screening and capable of screening plump pecans, half-plump pecans and non-plump pecans. The pecan plumpness screening device is easy to install, convenient to operate, suitable for being used inside, low in manufacture cost, convenient to maintain and low in maintenance cost, and various devices can be detached.

The invention discloses a method for developing an SSR (Simple Sequence Repeat) molecular mark of nibea albiflora for population identification. The method comprises the following steps: extracting RNA (Ribonucleic Acid) by using a nibea albiflora mixed tissue of a plurality of nibea albiflora, performing RNA sequencing, performing splicing to obtain a nibea albiflora expression gene sequence, and obtaining a potential SSR mark with a bioinformatics method; designing primers by utilizing an SSR flanking sequence, extracting genomic DNA (Deoxyribose Nucleic Acid) by using fins from a random sample, and screening the SSR primers; and performing PCR (Polymerase Chain Reaction) amplification on different nibea albiflora DNA templates by using each pair of the SSR primers, performing capillary electrophoresis detection on an amplification product by using a Qsep100 full-automatic nucleic acid analysis system, screening out primers with different amplification results, calculating allelic composition and allelic frequency by utilizing PopGene, and analyzing a nibea albiflora population relationship and a genetic structure. The SSR molecular mark method for identifying and verifying nibea albiflora groups, provided by the invention, is stable and reliable, is not influenced by ages, gender and growth seasons, and provides scientific bases for group analysis as well as species identification, breeding and evaluation of the nibea albiflora.

The invention discloses a computer medicine screening method based on a BFGS algorithm. The method comprises the following steps of 1) database establishment, wherein a data set cluster composed of 30,000-40,000 marine micromolecules is subjected to searching loop matching to form a micromolecule library with the similarity of 0-1; 2) database pre-processing, wherein micromolecule conformations, with the similarity of 1, in the micromolecule library obtained in step 1) are deleted; 3) molecular docking, wherein ligands are placed at active loci of a receptor, so that multiple ligands and one receptor are in molecular docking; 4) searching for conformations, wherein the BFGS algorithm is used for searching for the conformations, and when newly-generated conformations are identical to initial conformations or preset difference values of the newly-generated conformations and the initial conformations are smaller than or equal to 0.0001, searching for the conformations is stopped. According to the computer medicine screening method, by optimizing the algorithm, the time of searching for the low-energy conformations of the ligands and the receptor which are combined at the active loci of the receptor in the algorithm is shortened, and the effect and efficiency of virtual screening of a micromolecule database based on the method are improved.

The present invention provides a chip screening method and apparatus. The method comprises: calculating a first influence factor of each chip within a peripheral range of a to-be-determined chip, and summing the first influence factors of the chips within the peripheral range to obtain a sum of the influence factors; normalizing the first influence factor of each chip within the peripheral range based on the sum of the influence factors to obtain a second influence factor; summing the second influence factors of effective chips within the peripheral range to obtain a correlation factor NDCF of the peripheral chips of the to-be-determined chip; and if the NDCF of the to-be-determined chip meets predefined screening criteria, determining that the to-be-determined chip is a chip with a large potential failure rate. The present invention reduces chip screening costs.

The invention relates to the technical field of data processing, in particular to a patient data screening method and device, a storage medium and an electronic apparatus. The method comprises the steps of: acquiring current medical data; calculating difference value data between the current medical data and medical data in an inclusion / exclusion list; using an inclusion / exclusion condition to screen out medical data of patients meeting the inclusion / exclusion condition in the difference value data. The method and the device guarantee the accuracy of medical data of patients obtained through screening, achieve automatic screening of medical data of patients, increase screening efficiency and greatly reduce screening cost.

The invention discloses a method for screening myocardial ischemia resisting pharmacodynamic material basis of yang exhaustion treating decoction. The method comprises the following steps: 1, determining myocardial ischemia resisting endogenous metabolin of the yang exhaustion treating decoction and measuring the peak area; 2, determining in-vivo migration constituents in the yang exhaustion treating decoction and measuring the peak area; 3, performing correlation analysis on the peak area of the myocardial ischemia resisting endogenous metabolin of the yang exhaustion treating decoction and the peak area of the in-vivo migration constituents in the yang exhaustion treating decoction and screening out myocardial ischemia resisting pharmacodynamic material basis of the yang exhaustion treating decoction. The method disclosed by the invention is based on isoproterenol-induced rat myocardial ischemia model; an ultrahigh performance liquid chromatography-quadrupole rod-flight time mass spectrum non-targeted metabonomic technology and a targeted metabonomic technology based on an ultrahigh performance liquid chromatography-triple quadrupole rod mass spectrum are utilized to screen out 12 kinds of myocardial ischemia resisting pharmacodynamic material basis of the yang exhaustion treating decoction; thus, accuracy of the myocardial ischemia resisting pharmacodynamic material basis ofthe yang exhaustion treating decoction is improved, a screening period is shortened, and screening consumption cost is reduced.

The invention provides an electronic component fault diagnosis model based on data analysis and prediction. The electronic component fault diagnosis model comprises a data acquisition module, a data preprocessing module, a fault diagnosis model construction module, a prediction module and a prediction result output module. The fault diagnosis model construction module receives the 'clean' structured data set and then performs clustering to obtain a plurality of clusters. For each cluster, fractal geometry is applied to quantify the fractal dimension value of the cluster, the fractal dimensionvalue of each cluster is the fault feature of the cluster, and the value is used for judging the fault type existing in the batch components. Input data is stored in a storage module in a classified mode according to fault types. According to the method, the manual repetitive detection work is reduced, the music detection period is shortened, the screening cost is reduced, the production quality control potential of electronic components of manufacturers is excavated, the continuous improvement of the music ability and benefits is realized, high-quality and high-reliability electronic components are provided for models, and the reliability and stability of aerospace model products are ensured.

This invention provides one lithium battery balance and charging method, which comprises the following steps: monitoring series each lithium battery level or electricity difference; when any two lithium battery levels or volumes exceed certain value adopting series lithium battery simultaneous minimum level to make each battery volume zero; then continuing series charging to make each lithium battery achieve even charging status.