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Primer pair for screening mammalian experiment animal SNP (Single Nucleotide Polymorphism) markers and application thereof

A technology of experimental animals and primer pairs, applied in recombinant DNA technology, DNA/RNA fragments, etc., can solve the problems of cumbersome work and large cost investment, and achieve the effect of accelerating standardization and promoting standardization

Inactive Publication Date: 2015-08-26
SOUTH CHINA UNIV OF TECH
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0005] At present, there is no uniform standard for the development of genetic markers for experimental animals. The development of genetic markers for a single mammalian experimental animal often requires a large amount of preliminary screening work (such as genome sequencing, primer design for a single species, and routine sequencing, etc.), which is cumbersome and costly.

Method used

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  • Primer pair for screening mammalian experiment animal SNP (Single Nucleotide Polymorphism) markers and application thereof
  • Primer pair for screening mammalian experiment animal SNP (Single Nucleotide Polymorphism) markers and application thereof
  • Primer pair for screening mammalian experiment animal SNP (Single Nucleotide Polymorphism) markers and application thereof

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Experimental program
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Embodiment 1

[0019] 1. Experimental animal samples

[0020] Tissues from dogs, pigs, rabbits, rats, mice, and rhesus monkeys.

[0021] 2. Source of animal genome

[0022] The genome data of the six animal samples is the latest version of the genome of the species downloaded from Genebank, and the source of the genome data is shown in Table 1.

[0023] Table 1 Genome Data Sources

[0024] species

Genome URL

dog

http: / / www.ncbi.nlm.nih.gov / genome / 85

pig

http: / / www.ncbi.nlm.nih.gov / genome / 84

rabbit

http: / / www.ncbi.nlm.nih.gov / genome / ? term=rabbit

the rat

http: / / www.ncbi.nlm.nih.gov / genome / 73

mouse

http: / / www.ncbi.nlm.nih.gov / genome / 52

rhesus monkey

http: / / www.ncbi.nlm.nih.gov / genome / 215

[0025] 3. Source of animal tissue: Animal Genetics Laboratory of South China Agricultural University

[0026] 4. Target sequence screening

[0027] The present invention uses lastz as comparison software to compare ...

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Abstract

The invention discloses a primer pair for screening mammalian experiment animal SNP (Single Nucleotide Polymorphism) markers and application thereof. 30 pairs of primer pairs are provided, and sequences are represented from SEQ ID NO:1 to SEQ ID NO:60; each primer pair can be used for amplifying genomic sequences of experiment animals including dogs, pigs, rats, mice, rhesus monkeys and rabbits under the same condition; and sequences are represented from SEQ ID NO:61 to SEQ ID NO:240. Sequence species amplified by the same pair of primers have difference. The application of the primer pair can greatly reduce the screening cost of the mammalian experiment animal genetic markers and simplify the operation, so that the standardization of experiment animal genetic quality monitoring is facilitated.

Description

technical field [0001] The invention relates to developing a set of primer pairs that can be used for SNP marker screening of mammalian experimental animals and its application by utilizing the advantages of the whole genome biological information analysis technology, and using the method of PCR amplification and sanger sequencing to verify the primer pairs. Background technique [0002] At present, the domestic biomedical industry is developing rapidly. Experimental animals are an important supporting condition for the biomedical industry. The standardization of animal quality, especially the standardization of genetic quality, will directly affect the research and development level of biomedicine. However, due to the lack of unified and standardized genetic quality control standards, the genetic quality monitoring of experimental animals has gradually become a more prominent problem in its production and application. The technical bottleneck of genetic quality monitoring o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 杜红丽王菊芳柳明玉赵毅黄黎珍
Owner SOUTH CHINA UNIV OF TECH
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