147 results about "Animal Genetics" patented technology

The invention belongs to the technical field of animal genetic breeding, and particularly discloses an SNP (Single Nucleotide Polymorphism) chip for distinguishing five kinds of Hubei native cattle (yi-ling cattle, Dabie mountain cattle, troilin cattle, Enshi cattle and delta mountain cattle). Candidate SNP loci are obtained by combining whole genome sequencing and three selection signals (Tajima's D, FST and pi ratio), then the candidate loci are subjected to random forest scoring, the number of the needed SNP loci is further reduced, finally, an SNP locus combination with variety specificity is obtained, probes are designed according to the SNP loci and 60 bp sequences before and after the SNP loci, and the variety specificity is obtained. The SNP chip for distinguishing five kinds of Hubei native cattle can be developed, technical support is provided for variety breeding of Hubei native cattle, and meanwhile, compared with a traditional pedigree and microsatellite variety identification method, the SNP chip has the advantages of being high in accuracy and easy to operate.

The invention belongs to the technical field of livestock and poultry genetic markers and animal genetic breeding, and particularly relates to a structural variation molecular marker related to chicken secondary average feed intake and application of the structural variation molecular marker. The nucleotide sequence of the structural variation molecular marker provided by the invention is as shown in SEQ ID NO: 1, the structural variation molecular marker is located at 14th, 881, 990-14th, 882 and 295bp of a chicken chromosome 12, and the structural variation molecular marker is a deletion marker. The key genetic marker related to the chicken feeding behavior is provided, important information is provided for genetic breeding of the broiler chicken, meanwhile, broiler chicken varieties with different feeding behavior habits can be bred according to different markets and feeding environments, refined feeding management measures are formulated, and therefore the production efficiency and the feed utilization rate are improved, and the economic benefit is increased. And the economic benefit of the broiler industry is improved.

The invention discloses a Parkinson's disease animal model, a construction method and application, and relates to the field of animal genetic engineering and disease model construction. According to the model, seven mutation sites related to the human familial Parkinson's disease are introduced behind an initiation codon of a fourth exon of an endogenous SNCA gene, and the mutation sites are A18T, A29S, A30P, E46K, H50Q, G51D and A53T and have genotypes related to the human familial Parkinson's disease. The pig model shows nigra dopaminergic neuron reduction and cortical neuron reduction in the newborn period, and the core pathological process of the human Parkinson's disease can be systematically reproduced. The model can be widely applied to Parkinson's disease pathogenesis research, drug screening and cell therapy effect evaluation, and has important scientific research and preclinical application values.

The invention relates to the technical field of molecular markers and animal genetic breeding, in particular to an SNP marker influencing the content of porcine inosine and application of the SNP marker. The 10 SNP molecular markers closely related to the porcine inosine content are researched and determined on the basis of American-series purebred landrace, American-series purebred large white pigs and American-series purebred duroc, the dominant allele frequency can be increased generation by generation by optimizing the dominant allele of the SNP, the inosine content can be reduced, and the molecular markers can be used for identifying the porcine inosine content. The pig genetic improvement progress is accelerated, so that the economic benefit of breeding pig breeding is effectively improved.

The invention is applicable to the technical field of rabbit breeding, and provides a method for increasing the body weight of a rabbit based on a gene editing technology, which comprises the following steps: designing an action target sgRNA on a rabbit NR6A1 gene aiming at an NR6A1 p.P192S site; uniformly mixing the synthesized sgRNA and SpRY-CBE, injecting fertilized eggs, transplanting the fertilized eggs into fallopian tubes of receptor female rabbits, and feeding the receptor female rabbits until production; dNA is extracted from produced young rabbit ear tissues, PCR and sequencing are carried out, and if C at the fifth site in NR6A1 gene target sites is mutated into T, it is proved that single base mutation is obtained, and the NR6A1 gene edited rabbit is obtained. According to the invention, the rabbit variety with increased weight is successfully cultivated; a new thought of animal genetic breeding is developed by utilizing a gene editing technology, the method has application values in promoting improvement of genetic characters of rabbits and improving production performance and economic value of the rabbits, and powerful support is provided for high-quality development of the animal breeding industry in China.

The invention provides a construction method and application of a dilated cardiomyopathy animal model, belongs to the technical field of biological medicine and animal genetic model construction, and provides a novel dilated cardiomyopathy zebrafish model which is constructed by specifically overexpressing a truncated dnajb6b gene long isomer fragment in zebrafish myocardial cells. The nucleotide sequence of the gene segment is as shown in SEQ ID NO. 1; the invention also provides a construction method and application of the dilated cardiomyopathy zebra fish model, and further discovers application of 4-phenylbutyric acid in preparation of drugs for treating dilated cardiomyopathy.

The invention relates to the technical field of molecular markers and animal genetic breeding, in particular to an SNP marker influencing pig UMP content and application thereof. Whole genome re-sequencing and GWAS analysis are adopted, American-series purebred landrace pigs, American-series purebred large white pigs and American-series purebred duroc are used as research objects, seven SNP markers related to the pig UMP content are researched and determined, by optimizing dominant alleles of the SNP, the frequency of the dominant alleles can be increased generation by generation, the UMP content can be increased, and the pig UMP content can be improved. The pig genetic improvement progress is accelerated, so that the economic benefit of breeding pig breeding is effectively improved.

The invention discloses a pig SNP chip construction method based on gene regulatory network characteristics, and relates to the technical field of animal genetic breeding, and the pig SNP chip construction method comprises the following steps: constructing a pig tissue specificity multi-level gene regulatory network related to target traits and tissue types; performing function annotation and network comprehensive feature extraction on the whole genome SNP based on the gene regulation network; and carrying out score sorting and screening on the extracted network comprehensive characteristics, screening SNP variation sites with regulation function potential, constructing a pig SNP site set, and preparing the SNP chip. By introducing tissue / character related gene regulatory network information, functional priority ranking and screening are performed on SNP loci in a whole genome range, so that the character interpretation ability and breeding value estimation accuracy of SNP in a chip are remarkably improved.

The invention belongs to the technical field of animal genetic breeding, and provides an application of an SNP molecular marker related to a pig backfat thickness character. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 7, wherein the polymorphism of the eleventh basic group is C / T. The key gene SLC26A3 related to the backfat thickness of the large white pig is identified through whole genome deep sequencing, the genotype of the backfat thickness character of the large white pig is further detected through an SLC26A3 gene molecular marker, and the backfat thickness of the CC genotype large white pig is obviously higher than that of a CT / TT genotype pig. Therefore, the breeding of the CT / TT genotype individuals is beneficial to meeting the continuous demand of large-scale production on high-efficiency utilization of feed energy. The detection method provided by the invention is simple and easy to operate, can efficiently and quickly identify the back fat thickness character of the large white pig, improves the uniformity of the back fat thickness of the pig, and provides a scientific basis for early breeding of the pig.

The present invention relates to the field of animal genetic engineering technology, and provides a sgRNA for targeted editing of pig HNF1A gene and its application. The present invention determines the sequence of pig targeting site by homologous comparison based on the HNF1A gene mutation site hHNF1Ac.391C>T, which is typical of human MODY3 diabetes, and obtains pigs with pHNF1A c.391C>T point mutation by gene editing, and the genetic basis of the pig is consistent with that of humans. Pigs are similar to humans in terms of body size, anatomy, physiology, and susceptibility to obesity. As omnivorous animals, pigs have digestive systems and nutritional metabolic characteristics that are more similar to those of humans, and are suitable for translational medical application research on diseases related to human glucose and lipid metabolism disorders. Related diagnostic methods and treatment measures can be directly applied to humans.

The invention relates to the technical field of animal genetic breeding and genomics, and discloses a reference genome of a chromosome level of a Zhaotong Wujin pig and application of the reference genome. The high-quality chromosome level reference genome of the Zhaotong Wujin pig is constructed for the first time. The total length of the genome is about 2.66 Gb, the chromosome anchoring rate is more than 98%, Contig N50 is about 102Mb, and the integrity evaluation of the genome is more than 98%; 21507 protein coding genes are annotated in total. Compared with the existing reference sequence, the genome disclosed by the invention contains specific sequence variation and gene family information of local pig breeds, and provides a more accurate and comprehensive genetic blueprint. The genome resource can be widely applied to molecular mechanism research related to traits such as meat quality, reproductive capacity, growth speed, disease resistance and the like, molecular marker development, breeding chip design, quantitative trait locus (QTL) positioning, genetic evaluation, gene editing, transgenic breeding, germplasm resource protection and the like. The method has important application value in genetic improvement of Chinese local pig breeds.

This invention discloses a molecular marker for superovulation in cattle using the EXOC4 gene and its application method, belonging to the field of animal genetic engineering technology. By conducting correlation analysis between EXOC4 gene polymorphism and the effect of superovulation in cattle, the single nucleotide polymorphism at a specific site of the EXOC4 gene and its accurate identification method were clarified, and the influence of this site's genetic polymorphism on the effect of superovulation in cattle was determined. This provides an important theoretical basis and application prospect for using it as a molecular marker for auxiliary selection of bovine reproductive performance and applying it to genetic improvement in actual production.

The invention relates to the technical field of animal genetic breeding, and provides a double-character genome selection platform based on local genetic correlation (LGC). The platform is composed of a phenotype data processing module, a genotype data processing module and a genome selection module (comprising a model selection sub-module and a parameter selection sub-module). The platform performs quality control, data normality test and correction on phenotypic data; performing quality control, sequence alignment, variation detection and genotype filling on the genotype data; and identifying whole genome local genetic correlation, checking the significance of LGC, selecting a model based on a whole genome LGC estimation result of a character pair and a corresponding P value, setting a corresponding model parameter threshold, and realizing section weighted double-character genome selection analysis. According to the method, the genetic evaluation accuracy of complex characters, especially low heritability characters, can be effectively improved, and an efficient and stable genome selection tool is provided for livestock and poultry breeding.

The invention discloses a mandarin fish medullary tissue cell line and a construction method and application thereof. The invention belongs to the technical field of aquatic organism cells, and discloses a mandarin fish medulla oblongata cell line MFSB, which is preserved in China Center for Type Culture Collection on December 4, 2024, has a preservation number of CCTCC NO: C2024376, and is named as mandarin fish medulla oblongata cell line MFSB. The construction method comprises the steps of primary culture of mandarin fish medulla oblongata tissue cells, subculture, cryopreservation and resuscitation of the cells and the like, and the mandarin fish medulla oblongata cell line MFSB has the advantages of stable subculture, strong cell line division ability, fast growth speed and good stability, can be used as a cell model or an experimental tool to be applied to scientific research and animal genetic breeding, and has wide application prospects. The probe has broad-spectrum sensitivity to mandarin fish infectious spleen and kidney necrosis virus, mandarin fish double-segment RNA virus, mandarin fish frog iridovirus and mandarin fish rhabdovirus, and can be well applied to separation, culture and detection of mandarin fish virus and research and development of vaccines.

The present application relates to the field of molecular markers and animal genetic breeding technology, and particularly relates to a SNP marker affecting pig purine base content and application thereof. Based on three test pig populations of American purebred Landrace, American purebred Large White and American purebred Duroc, the present application uses pig whole genome resequencing and GWAS analysis to research and determine 15 SNP markers significantly affecting pig purine base content. By selecting the advantageous alleles of the above SNP markers, the frequency of the advantageous alleles can be increased generation by generation, the corresponding purine content can be reduced or increased, the progress of pig genetic improvement can be accelerated, and the economic benefits of pig breeding can be effectively improved.

The application provides application of a SNP genetic marker affecting chicken body weight at the onset of lay in genetic breeding of laying hens, and belongs to the field of animal genetic breeding and biotechnology.The SNP genetic marker affecting chicken body weight at the onset of lay comprises bw1egg_1 and / or bw1egg_2; the Ensembl number of the bw1egg_1 is rs318020581, corresponding to the 76311052th position of the positive strand of chromosome 4 in the chicken reference genome bGalGal1.mat.broiler.GRCg7b sequence published in NCBI, belonging to the 3rd intron of the gene C1QTNF7, and the base at the position is T or G; the Ensembl number of the bw1egg_2 is rs313708699, corresponding to the 74731889th position of the positive strand of chromosome 4 in the chicken reference genome bGalGal1.mat.broiler.GRCg7b sequence published in NCBI, belonging to the 8th intron of the gene SLIT2, and the base at the position is T or C.Both the bw1egg_1 and the bw1egg_2 are helpful to genetically improve the body weight at the onset of lay, and when applied to genetic breeding of chickens, are favorable to improving the body weight at the onset of lay of the laying hens and obtaining a laying hen breed with excellent performance in uniformity of the body weight at the onset of lay.

This invention relates to the fields of molecular markers and animal genetic breeding technology, specifically to a molecular marker located on chromosome 4 of pigs that is associated with body size and carcass traits, and its application. Using the F7 generation of a chimeric family as the research object, this invention employs whole-genome resequencing and GWAS analysis to study and identify the molecular markers located on chromosome 4 of pigs associated with body size and carcass traits. By optimizing the dominant alleles of these molecular markers, the frequency of dominant alleles can be increased generation by generation, enabling rapid and accurate selection of body size and / or carcass traits, accelerating the progress of pig genetic improvement, and thus effectively improving the economic benefits of pig breeding.

The invention discloses a detection method and application of chicken Columbia feather type black and white tail feather genes. Belongs to the technical field of animal genetic breeding. According to the invention, the A / G mutation at the 10892295 position of the chicken Z chromosome genome is associated with the chicken Columbia feather, and the T / C mutation at the 1341621 position of the chicken 34 chromosome genome is associated with the black and white tail of the chicken. A primer pair K and a primer pair P for detecting genotypes of Columbia feathers and black and white tail feathers of chickens are constructed according to the SNP loci; the gene typing detection method which is matched with the method, efficient, low in cost and suitable for large-scale groups is developed, the provided method is easy to operate, rapid in detection and accurate in result, the characters of Columbia feathers and black tail feathers can be more efficiently screened in breeding practice, the breeding progress is accelerated, the breeding cost is saved, and the method is suitable for popularization and application. And a rapid molecular breeding method is provided for breeding of characters of Columbia-feather black-tail chickens.

The application belongs to the technical field of animal genetic engineering, and particularly discloses a construction method of C1R gene humanized animal cells and animal models and application thereof. The construction method of the C1R gene humanized animal cells comprises the following steps: introducing a human C1R gene into non-human animal cells, so that the human C1R gene is expressed in the non-human animal cells to produce humanized C1R protein, and meanwhile, the expression of an endogenous C1ra gene in the non-human animal cells is reduced or eliminated. The application preferably adopts CRISPR / Cas9 gene editing technology to accurately insert a genomic sequence encoding a human C1R mature protein into a 2nd exon region of a mouse C1ra gene, while retaining a mouse source promoter, a 5' untranslated region (UTR) and a signal peptide sequence. A mouse cell or model capable of specifically interacting with an anti-human C1R antibody is successfully constructed.

The application provides a method for constructing an animal model of dilated cardiomyopathy and application, belongs to the technical field of biological medicine and animal genetic model construction, provides a new zebrafish model of dilated cardiomyopathy, is constructed by specifically overexpressing a truncated dnajb6b gene long isoform fragment in zebrafish myocardial cells, the nucleotide sequence of the gene fragment is shown as SEQ ID NO. 1, and the method for constructing the zebrafish model of dilated cardiomyopathy and application are provided, and it is further found that 4-phenylbutyric acid is applied to the preparation of a drug for treating dilated cardiomyopathy.

The application relates to the field of animal genetic breeding technology, and discloses a local pig breed multi-node block chain collaborative breeding method. According to the method, key data abstracts, evaluation results, model versions and breeding decisions are chained and left marks in a window period through a permissioned consortium chain and a consensus verification mechanism, the source is clear, tamper-proof and auditable, the problem that data standards are different, difficult to trace and difficult to trust in traditional multi-subject collaboration is solved, in addition, an AI multi-factor breeding evaluation system can simultaneously process multiple traits such as meat quality, growth, reproduction, health resistance and environmental management factors, can depict the nonlinear correlation between complex traits, can improve the evaluation precision and stability of the comprehensive genetic value of local pig breeds, can reduce the risk of decision-making errors caused by single-point data or single-algorithm deviation, can realize cross-node alignment and joint use of dispersed data through a rolling window and a labeled archive, and can make up for the short board of insufficient samples of a single breeding unit.

The application provides a genetic marker associated with chicken intestinal length in a KCNIP4 gene and an application thereof, and belongs to the fields of animal genetics and breeding and biotechnology.The genetic marker comprises IL_tag1 or IL_tag2; the Ensembl number of the IL_tag1 is rs316532738, corresponds to the sequence of a positive strand of a chromosome No.4 of a chicken reference genome bGalGal1.mat.broiler.GRCg7b published by NCBI, is located in the 1st intron of a gene KCNIP4, and the base at the position is T or C; the Ensembl number of the IL_tag2 is rs316953671.The genetic marker is helpful to genetically improve the intestinal length of a laying hen, is applied to the genetic breeding of a chicken, and is favorable to improving intestinal traits and obtaining a laying hen variety with better nutrient absorption.

The invention is applicable to the technical field of animal genetic engineering, and provides a DHX36 gene molecular marker related to bovine sperm motility traits and application thereof, the DHX36 gene molecular marker is a 231st locus of a bovine DHX36 gene segment as shown in a sequence table SEQ ID NO: 1, and the locus has a C-G base mutation. Based on the DHX36 gene molecular marker, the genotype is judged through sequence determination, and the genotype can be associated with the normal and unfrozen activity traits of bovine sperms. An analysis result shows that the bovine sperms of individuals with different genotypes are normal and have significant differences in vitality after unfreezing. According to the invention, the specific primer group is utilized to obtain the bovine DHX36 gene segment related to the normal and unfrozen activity traits of bovine sperms, and the specific SNP site in the segment is taken as the molecular marker, so that a theoretical basis and specific application are provided for marker-assisted selection of cattle.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular genetic marker of an NFE2L1 gene related to a chicken feed conversion rate and application, and belongs to the technical field of animal genetic breeding and molecular biology. The SNP molecular genetic marker is located at the 6493284 site of a chromosome 27, the sequence number is rs731344472, and the site is G > T mutation. Experiments prove that the SNP molecular genetic marker chr27-6493284 provided by the invention is remarkably related to the feed conversion rate character of the yellow-feathered broiler chicken, can be applied to breeding of the yellow-feathered broiler chicken with low feed conversion rate, and can effectively reduce the feed consumption in the production process and improve the economic benefits and competitiveness of enterprises; the method can be used for efficiently screening yellow feather broilers of individuals or parents with excellent characters of feed conversion rate.

The invention relates to the technical field of molecular biology and animal genetic breeding, and aims to provide a method for identification or auxiliary identification of sheep eye muscle area traits, the method comprises the following steps: detecting the genotype of Chr5: g.92532941 locus in a to-be-detected sheep genome; wherein the basic group of the Chr5: g.92532941 site is G or A, and the genotype is GG, GA or AA; and based on the genotype, identifying or assisting in identifying the eye muscle area character of the sheep to be detected. The eye muscle area character of the to-be-detected sheep with the genotype of AA is superior to or assisted to the eye muscle area character of the to-be-detected sheep with the genotype of GG; or the ocular muscle area character of the to-be-detected sheep with the genotype of AA is superior to or assisted superior to the ocular muscle area character of the to-be-detected sheep with the genotype of GA. The invention discloses the obvious association between the SNP of the sheep Chr5: g.92532941 locus (Ggt; A) and the eye muscle area character of the 12-month-old ram for the first time, it is clear that the AA genotype is a dominant genotype, and a reliable genetic target is provided for molecular breeding.

The invention relates to the technical field of biotechnology and animal genetic breeding, and aims at providing application of an SLC2A9 gene in identifying the chicken ovary state. The second purpose of the invention is to provide an application of the SLC2A9 gene or the expression product thereof as a marker in identifying or evaluating the follicular atresia degree of chicken. The third purpose of the invention is to provide the application of the SLC2A9 gene or the expression product of the SLC2A9 gene as a marker in identifying or evaluating the senescence state of the chicken ovary. The invention finds that the SLC2A9 gene or the expression product thereof can be used as a specific biomarker for evaluating the chicken ovarian development state, follicular atresia degree and ovarian aging degree. By detecting the expression level, the objective and quantitative evaluation of the ovarian physiological status of the individual chicken can be realized, and the limitation of only depending on external phenotype observation is overcome.

The invention provides application of an SNP (Single Nucleotide Polymorphism) molecular marker influencing the first laying day age of chickens in laying hen breeding, and belongs to the technical field of animal genetic breeding and biologication.The SNP molecular marker influencing the first laying day age of the chickens comprises AFEchr161 and / or AFEchr162; the Ensemb l number of the AFEchr161 is rs316781747, corresponds to the 2078538th site of a positive-sense strand of a chromosome 16 in a chicken reference genome bGa < l1 >. Mat.broi ler.GRCg7b sequence disclosed in NCBI (National Center of Biotechnology Information), and belongs to the first intron of a gene TRIM7.2, and the basic group at the site is G or C; the Ensemb l number of the AFEchr162 is rs15788024, corresponds to the 2121574 site of a positive-sense strand of a chromosome 16 in a chicken reference genome bGa < 1 >. Mat.broi ler.GRCg7b sequence disclosed in NCBI (National Center of Biotechnology Information), and belongs to the fourth intron of a gene TRIM39.2, and the basic group at the site is T or C. The invention also discloses a method for preparing the AFEchr162. The AFEchr161 and the AFEchr162 are both helpful for advancing the first laying day age of the laying hens from the aspect of heredity, and the AFEchr161 and the AFEchr162 are applied to genetic breeding of chickens, so that the first laying day age of the laying hens is shortened, and the requirements of an egg product market on green-shell laying hen varieties are met.

The invention discloses a muscle cell efficient growth gene knock-in cell line construction method based on a CRISPR-Cas9 system, and belongs to the crossing field of gene editing and biotechnology. Through a CRISPR-Cas9 mediated gene targeting insertion technology, fixed-point integration of muscle efficient growth related functional genes is realized in bovine fetal fibroblasts, and a gene overexpression cell line with stable hereditary characteristics is successfully constructed. The cell line can be used as a nuclear donor, and precise creation of high-muscle-production-performance gene editing beef cattle is realized by combining a somatic cell nuclear transfer technology and an embryo transfer technology. The key technical support is provided for beef cattle molecular design breeding by directionally enhancing the cattle muscle development core regulation and control network. The genetic improvement new material cultivated based on the technology is expected to significantly improve the beef cattle muscle growth efficiency and industrial economic benefits, and shows important application value and industrialization prospects in the field of agricultural animal genetic engineering.

The present invention belongs to the technical field of livestock and poultry genetic markers and animal genetic breeding, and specifically relates to a SNP molecular marker related to the average feeding time of chickens and its application. The SNP molecular markers are located at 1,035,978bp and 1,035,986bp of chicken chromosome 2, respectively. The candidate genes are <h2 style=";text-align:left;direction:ltr">GIMAP7 Gene. The SNP molecular markers associated with average feeding time per chicken provided by the present invention provide key genetic information for broiler genetic breeding, facilitating the precise selection of populations with excellent average feeding time traits during broiler breeding, improving broiler feeding efficiency and production performance, and enhancing the economic benefits of the broiler industry. The discovery of SNP molecular markers associated with average feeding time per chicken also provides an important research foundation for in-depth studies of the genetic mechanisms of chicken feeding behavior, helping to promote the development of livestock and poultry genetic breeding.

The invention belongs to the technical field of animal molecular biology and animal genetic breeding, and particularly relates to a buffalo whole genome 20k liquid-phase SNP chip and a design method and application thereof, and the chip comprises 24893 SNP molecular markers; according to the method, four selection signal analysis (FST, Piratio, XP-CLR and XP-EHH) are jointly applied to screen 11 buffalo group comparison combinations, it is ensured that the sites have high information content and group universality, through multiple rounds of quality control and function annotation, finally 24893 SNP sites are carefully selected, the whole genome is covered in a balanced mode, and the redundancy is remarkably reduced; based on 115 sample data of 11 main Chinese buffalo varieties (including marsh type and river type), the chip is ensured to be suitable for multi-variety buffalo groups, and the application advantages of variety identification, genetic analysis, genetic diversity monitoring and the like are highlighted.