99 results about "Animal Genetics" patented technology

The invention discloses a Parkinson's disease animal model, a construction method and application, and relates to the field of animal genetic engineering and disease model construction. According to the model, seven mutation sites related to the human familial Parkinson's disease are introduced behind an initiation codon of a fourth exon of an endogenous SNCA gene, and the mutation sites are A18T, A29S, A30P, E46K, H50Q, G51D and A53T and have genotypes related to the human familial Parkinson's disease. The pig model shows nigra dopaminergic neuron reduction and cortical neuron reduction in the newborn period, and the core pathological process of the human Parkinson's disease can be systematically reproduced. The model can be widely applied to Parkinson's disease pathogenesis research, drug screening and cell therapy effect evaluation, and has important scientific research and preclinical application values.

The invention discloses a pig SNP chip construction method based on gene regulatory network characteristics, and relates to the technical field of animal genetic breeding, and the pig SNP chip construction method comprises the following steps: constructing a pig tissue specificity multi-level gene regulatory network related to target traits and tissue types; performing function annotation and network comprehensive feature extraction on the whole genome SNP based on the gene regulation network; and carrying out score sorting and screening on the extracted network comprehensive characteristics, screening SNP variation sites with regulation function potential, constructing a pig SNP site set, and preparing the SNP chip. By introducing tissue / character related gene regulatory network information, functional priority ranking and screening are performed on SNP loci in a whole genome range, so that the character interpretation ability and breeding value estimation accuracy of SNP in a chip are remarkably improved.

The invention belongs to the technical field of animal genetic breeding, and provides an application of an SNP molecular marker related to a pig backfat thickness character. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO: 7, wherein the polymorphism of the eleventh basic group is C / T. The key gene SLC26A3 related to the backfat thickness of the large white pig is identified through whole genome deep sequencing, the genotype of the backfat thickness character of the large white pig is further detected through an SLC26A3 gene molecular marker, and the backfat thickness of the CC genotype large white pig is obviously higher than that of a CT / TT genotype pig. Therefore, the breeding of the CT / TT genotype individuals is beneficial to meeting the continuous demand of large-scale production on high-efficiency utilization of feed energy. The detection method provided by the invention is simple and easy to operate, can efficiently and quickly identify the back fat thickness character of the large white pig, improves the uniformity of the back fat thickness of the pig, and provides a scientific basis for early breeding of the pig.

The invention relates to the technical field of animal genetic breeding and genomics, and discloses a reference genome of a chromosome level of a Zhaotong Wujin pig and application of the reference genome. The high-quality chromosome level reference genome of the Zhaotong Wujin pig is constructed for the first time. The total length of the genome is about 2.66 Gb, the chromosome anchoring rate is more than 98%, Contig N50 is about 102Mb, and the integrity evaluation of the genome is more than 98%; 21507 protein coding genes are annotated in total. Compared with the existing reference sequence, the genome disclosed by the invention contains specific sequence variation and gene family information of local pig breeds, and provides a more accurate and comprehensive genetic blueprint. The genome resource can be widely applied to molecular mechanism research related to traits such as meat quality, reproductive capacity, growth speed, disease resistance and the like, molecular marker development, breeding chip design, quantitative trait locus (QTL) positioning, genetic evaluation, gene editing, transgenic breeding, germplasm resource protection and the like. The method has important application value in genetic improvement of Chinese local pig breeds.

This invention discloses a molecular marker for superovulation in cattle using the EXOC4 gene and its application method, belonging to the field of animal genetic engineering technology. By conducting correlation analysis between EXOC4 gene polymorphism and the effect of superovulation in cattle, the single nucleotide polymorphism at a specific site of the EXOC4 gene and its accurate identification method were clarified, and the influence of this site's genetic polymorphism on the effect of superovulation in cattle was determined. This provides an important theoretical basis and application prospect for using it as a molecular marker for auxiliary selection of bovine reproductive performance and applying it to genetic improvement in actual production.

The invention relates to the technical field of animal genetic breeding, and provides a double-character genome selection platform based on local genetic correlation (LGC). The platform is composed of a phenotype data processing module, a genotype data processing module and a genome selection module (comprising a model selection sub-module and a parameter selection sub-module). The platform performs quality control, data normality test and correction on phenotypic data; performing quality control, sequence alignment, variation detection and genotype filling on the genotype data; and identifying whole genome local genetic correlation, checking the significance of LGC, selecting a model based on a whole genome LGC estimation result of a character pair and a corresponding P value, setting a corresponding model parameter threshold, and realizing section weighted double-character genome selection analysis. According to the method, the genetic evaluation accuracy of complex characters, especially low heritability characters, can be effectively improved, and an efficient and stable genome selection tool is provided for livestock and poultry breeding.

The present application relates to the field of molecular markers and animal genetic breeding technology, and particularly relates to a SNP marker affecting pig purine base content and application thereof. Based on three test pig populations of American purebred Landrace, American purebred Large White and American purebred Duroc, the present application uses pig whole genome resequencing and GWAS analysis to research and determine 15 SNP markers significantly affecting pig purine base content. By selecting the advantageous alleles of the above SNP markers, the frequency of the advantageous alleles can be increased generation by generation, the corresponding purine content can be reduced or increased, the progress of pig genetic improvement can be accelerated, and the economic benefits of pig breeding can be effectively improved.

The application provides application of a SNP genetic marker affecting chicken body weight at the onset of lay in genetic breeding of laying hens, and belongs to the field of animal genetic breeding and biotechnology.The SNP genetic marker affecting chicken body weight at the onset of lay comprises bw1egg_1 and / or bw1egg_2; the Ensembl number of the bw1egg_1 is rs318020581, corresponding to the 76311052th position of the positive strand of chromosome 4 in the chicken reference genome bGalGal1.mat.broiler.GRCg7b sequence published in NCBI, belonging to the 3rd intron of the gene C1QTNF7, and the base at the position is T or G; the Ensembl number of the bw1egg_2 is rs313708699, corresponding to the 74731889th position of the positive strand of chromosome 4 in the chicken reference genome bGalGal1.mat.broiler.GRCg7b sequence published in NCBI, belonging to the 8th intron of the gene SLIT2, and the base at the position is T or C.Both the bw1egg_1 and the bw1egg_2 are helpful to genetically improve the body weight at the onset of lay, and when applied to genetic breeding of chickens, are favorable to improving the body weight at the onset of lay of the laying hens and obtaining a laying hen breed with excellent performance in uniformity of the body weight at the onset of lay.

This invention relates to the fields of molecular markers and animal genetic breeding technology, specifically to a molecular marker located on chromosome 4 of pigs that is associated with body size and carcass traits, and its application. Using the F7 generation of a chimeric family as the research object, this invention employs whole-genome resequencing and GWAS analysis to study and identify the molecular markers located on chromosome 4 of pigs associated with body size and carcass traits. By optimizing the dominant alleles of these molecular markers, the frequency of dominant alleles can be increased generation by generation, enabling rapid and accurate selection of body size and / or carcass traits, accelerating the progress of pig genetic improvement, and thus effectively improving the economic benefits of pig breeding.

The invention discloses a detection method and application of chicken Columbia feather type black and white tail feather genes. Belongs to the technical field of animal genetic breeding. According to the invention, the A / G mutation at the 10892295 position of the chicken Z chromosome genome is associated with the chicken Columbia feather, and the T / C mutation at the 1341621 position of the chicken 34 chromosome genome is associated with the black and white tail of the chicken. A primer pair K and a primer pair P for detecting genotypes of Columbia feathers and black and white tail feathers of chickens are constructed according to the SNP loci; the gene typing detection method which is matched with the method, efficient, low in cost and suitable for large-scale groups is developed, the provided method is easy to operate, rapid in detection and accurate in result, the characters of Columbia feathers and black tail feathers can be more efficiently screened in breeding practice, the breeding progress is accelerated, the breeding cost is saved, and the method is suitable for popularization and application. And a rapid molecular breeding method is provided for breeding of characters of Columbia-feather black-tail chickens.

The application belongs to the technical field of animal genetic engineering, and particularly discloses a construction method of C1R gene humanized animal cells and animal models and application thereof. The construction method of the C1R gene humanized animal cells comprises the following steps: introducing a human C1R gene into non-human animal cells, so that the human C1R gene is expressed in the non-human animal cells to produce humanized C1R protein, and meanwhile, the expression of an endogenous C1ra gene in the non-human animal cells is reduced or eliminated. The application preferably adopts CRISPR / Cas9 gene editing technology to accurately insert a genomic sequence encoding a human C1R mature protein into a 2nd exon region of a mouse C1ra gene, while retaining a mouse source promoter, a 5' untranslated region (UTR) and a signal peptide sequence. A mouse cell or model capable of specifically interacting with an anti-human C1R antibody is successfully constructed.

The application provides a method for constructing an animal model of dilated cardiomyopathy and application, belongs to the technical field of biological medicine and animal genetic model construction, provides a new zebrafish model of dilated cardiomyopathy, is constructed by specifically overexpressing a truncated dnajb6b gene long isoform fragment in zebrafish myocardial cells, the nucleotide sequence of the gene fragment is shown as SEQ ID NO. 1, and the method for constructing the zebrafish model of dilated cardiomyopathy and application are provided, and it is further found that 4-phenylbutyric acid is applied to the preparation of a drug for treating dilated cardiomyopathy.

The application relates to the field of animal genetic breeding technology, and discloses a local pig breed multi-node block chain collaborative breeding method. According to the method, key data abstracts, evaluation results, model versions and breeding decisions are chained and left marks in a window period through a permissioned consortium chain and a consensus verification mechanism, the source is clear, tamper-proof and auditable, the problem that data standards are different, difficult to trace and difficult to trust in traditional multi-subject collaboration is solved, in addition, an AI multi-factor breeding evaluation system can simultaneously process multiple traits such as meat quality, growth, reproduction, health resistance and environmental management factors, can depict the nonlinear correlation between complex traits, can improve the evaluation precision and stability of the comprehensive genetic value of local pig breeds, can reduce the risk of decision-making errors caused by single-point data or single-algorithm deviation, can realize cross-node alignment and joint use of dispersed data through a rolling window and a labeled archive, and can make up for the short board of insufficient samples of a single breeding unit.

The application provides a genetic marker associated with chicken intestinal length in a KCNIP4 gene and an application thereof, and belongs to the fields of animal genetics and breeding and biotechnology.The genetic marker comprises IL_tag1 or IL_tag2; the Ensembl number of the IL_tag1 is rs316532738, corresponds to the sequence of a positive strand of a chromosome No.4 of a chicken reference genome bGalGal1.mat.broiler.GRCg7b published by NCBI, is located in the 1st intron of a gene KCNIP4, and the base at the position is T or C; the Ensembl number of the IL_tag2 is rs316953671.The genetic marker is helpful to genetically improve the intestinal length of a laying hen, is applied to the genetic breeding of a chicken, and is favorable to improving intestinal traits and obtaining a laying hen variety with better nutrient absorption.

The invention is applicable to the technical field of animal genetic engineering, and provides a DHX36 gene molecular marker related to bovine sperm motility traits and application thereof, the DHX36 gene molecular marker is a 231st locus of a bovine DHX36 gene segment as shown in a sequence table SEQ ID NO: 1, and the locus has a C-G base mutation. Based on the DHX36 gene molecular marker, the genotype is judged through sequence determination, and the genotype can be associated with the normal and unfrozen activity traits of bovine sperms. An analysis result shows that the bovine sperms of individuals with different genotypes are normal and have significant differences in vitality after unfreezing. According to the invention, the specific primer group is utilized to obtain the bovine DHX36 gene segment related to the normal and unfrozen activity traits of bovine sperms, and the specific SNP site in the segment is taken as the molecular marker, so that a theoretical basis and specific application are provided for marker-assisted selection of cattle.

The invention relates to the technical field of molecular biology and animal genetic breeding, and aims to provide a method for identification or auxiliary identification of sheep eye muscle area traits, the method comprises the following steps: detecting the genotype of Chr5: g.92532941 locus in a to-be-detected sheep genome; wherein the basic group of the Chr5: g.92532941 site is G or A, and the genotype is GG, GA or AA; and based on the genotype, identifying or assisting in identifying the eye muscle area character of the sheep to be detected. The eye muscle area character of the to-be-detected sheep with the genotype of AA is superior to or assisted to the eye muscle area character of the to-be-detected sheep with the genotype of GG; or the ocular muscle area character of the to-be-detected sheep with the genotype of AA is superior to or assisted superior to the ocular muscle area character of the to-be-detected sheep with the genotype of GA. The invention discloses the obvious association between the SNP of the sheep Chr5: g.92532941 locus (Ggt; A) and the eye muscle area character of the 12-month-old ram for the first time, it is clear that the AA genotype is a dominant genotype, and a reliable genetic target is provided for molecular breeding.

The invention relates to the technical field of biotechnology and animal genetic breeding, and aims at providing application of an SLC2A9 gene in identifying the chicken ovary state. The second purpose of the invention is to provide an application of the SLC2A9 gene or the expression product thereof as a marker in identifying or evaluating the follicular atresia degree of chicken. The third purpose of the invention is to provide the application of the SLC2A9 gene or the expression product of the SLC2A9 gene as a marker in identifying or evaluating the senescence state of the chicken ovary. The invention finds that the SLC2A9 gene or the expression product thereof can be used as a specific biomarker for evaluating the chicken ovarian development state, follicular atresia degree and ovarian aging degree. By detecting the expression level, the objective and quantitative evaluation of the ovarian physiological status of the individual chicken can be realized, and the limitation of only depending on external phenotype observation is overcome.

The invention belongs to the technical field of animal molecular biology and animal genetic breeding, and particularly relates to a buffalo whole genome 20k liquid-phase SNP chip and a design method and application thereof, and the chip comprises 24893 SNP molecular markers; according to the method, four selection signal analysis (FST, Piratio, XP-CLR and XP-EHH) are jointly applied to screen 11 buffalo group comparison combinations, it is ensured that the sites have high information content and group universality, through multiple rounds of quality control and function annotation, finally 24893 SNP sites are carefully selected, the whole genome is covered in a balanced mode, and the redundancy is remarkably reduced; based on 115 sample data of 11 main Chinese buffalo varieties (including marsh type and river type), the chip is ensured to be suitable for multi-variety buffalo groups, and the application advantages of variety identification, genetic analysis, genetic diversity monitoring and the like are highlighted.

The present application relates to a kind of construction method of atherosclerosis humanized mouse model, belong to genetic engineering and genetic modification technical field.The present application is first in ApoE and Ldlr double gene knockout mouse background and obtains NOD-4G gene knockout mouse by knockouting Prkdc and Il2rg gene in mouse, humanized atherosclerosis model is constructed by injecting human umbilical cord blood CD133+stem cell and high-fat feed feeding.Through to atherosclerosis degree analysis, it is found that the aorta and aortic root of the humanized atherosclerosis model constructed have a large number of plaque formation, and contain human CD45 + Leukocyte.This atherosclerosis humanized mouse model provides better animal genetic model for the analysis of the role of human immune cells in the occurrence and development of atherosclerosis disease, and has important significance for the research of human disease and the development of new therapy.

The invention discloses a method for auxiliary identification of Duroc pig growth traits based on a BTG1 region SNP marker, and belongs to the technical field of animal genetic breeding and reproduction. Comprising the following steps: step S1, acquiring a growth trait record of a Duroc pig to be detected, performing data preprocessing, and calculating genetic correlation and phenotypic correlation between heritability and growth traits based on a pedigree; step S2, performing genetic typing on the Duroc pig to be detected, and detecting genotypes of the Duroc pig in a BTG1 region and other key SNP sites; s3, carrying out auxiliary identification on the growth traits of the to-be-detected Duroc pigs on the basis of a pre-established relationship between genotypes and the growth traits; and S4, selecting a corresponding individual as a breeding object according to an auxiliary identification result. The method can be used for early screening and genetic improvement of growth traits such as different day-age weights and staged average daily gain of Duroc pigs, and is suitable for breeding pig breeding practice of large-scale pig farms, live pig breeding enterprises and scientific research institutions.

This invention belongs to the field of animal genetics and breeding technology, specifically disclosing an SNP chip for distinguishing five native Hubei yellow cattle breeds (Yiling Yellow Cattle, Dabie Mountain Yellow Cattle, Yunba Yellow Cattle, Enshi Yellow Cattle, and Sanjiaoshan Yellow Cattle). This invention combines whole-genome sequencing and three selection signals (Tajima's D, FST, and π ratio) to obtain candidate SNP loci. Random forest scoring is then applied to these candidate loci to further reduce the number of required SNP loci, ultimately obtaining breed-specific SNP loci combinations. Probes are designed based on these SNP loci and their preceding and following 60 bp sequences, enabling the development of an SNP chip for distinguishing the five native Hubei yellow cattle breeds. This provides technical support for breed selection of native Hubei yellow cattle and, compared to traditional pedigree and microsatellite identification methods, offers advantages in high accuracy and ease of operation.

The invention discloses an NDUFS2 gene heterozygous editing pig as well as a construction method and application thereof, and relates to the field of animal gene engineering and disease model construction. The eighth exon of the NDUFS2 gene of the pig is deleted or mutated, and presents an NDUFS2 heterozygous genotype; in the newborn period, the number of neurons of the middle cerebral cortical layer and the number of dopaminergic neurons are obviously reduced, the number of neurons of the adult cortical layer is continuously reduced along with mitochondrial swelling and crest fracture, and after MPTP treatment, the number of neurons of the adult cortical layer is disordered in motion trail, and the body tremor index is increased. The pig model not only can be used for revealing the action mechanism of the NDUFS2 defect in neurodegenerative diseases (such as Parkinson's disease), but also can be used as an important tool for screening new drugs, evaluating treatment means and researching mitochondrial diseases.

The application discloses a kind of integrated hybrid information purebred genome prediction method, belong to animal genetic breeding and propagation technical field.The population genetic dataset comprising purebred population A, B and hybrid population CB is constructed, and the population genetic differentiation is verified by principal component analysis and genetic differentiation index;After the genotypes of purebred and hybrid population CB are phased, the ancestral information inference method is used to determine the origin information of each allele and process missing sites;Based on the origin information of allele, additive effect partial kinship correlation matrix and dominant effect partial kinship correlation matrix are constructed;The above matrix is integrated into three-trait mixed linear model, forming the prediction model that simultaneously incorporates purebred and hybrid population genetic information;The application distinguishes the breed origin of allele and quantifies its additive and dominant effect, significantly improves the prediction accuracy of purebred genome breeding value, is suitable for the traits difficult to directly determine in purebred population, and provides reliable technical support for animal breeding.

The invention discloses a ruminant parenchymal hepatic cell subtype analysis system, which belongs to the technical field of bioinformatics and animal genetics, and comprises a data processing module, which is used for executing cell typing, cell cycle analysis, quasi-time sequence analysis and function enrichment analysis based on single cell sequencing data, and integrating the multi-dimensional analysis results based on a weighted decision rule to carry out cross validation and identification on the subtype of the parenchymal hepatic cells. The method provided by the invention overcomes the defect that in the prior art, human and mouse cells are directly used for marking ruminants to cause inaccurate subtypes of the parenchymal hepatic cells, and provides a reliable tool for accurate identification of subtypes of the parenchymal hepatic cells of the ruminants by establishing a set of special analysis system.

The invention belongs to the technical field of animal genetic breeding, and particularly relates to a fast-growing high-quality black pig and a breeding method thereof, and the method comprises the following steps: (1) taking Suzhou pigs, Huai pigs and Berkshire pigs as parents, and carrying out three-time hybridization, two-time backcross and cross-cross fixation to produce a black pig basic breeding pig group containing 37.5% Berkshire lineage; and (2) carrying out closed breeding and subculture breeding on the black pig basic breeding pig group to breed a high-quality black pig new germplasm containing 37.5% of Berkshire pig lineage. According to the method, 37.5% of foreign boar blood lineage is introduced through a specific hybridization mode for high-quality local boar resources in China, on the basis that the performance characteristics of good local pork quality, high reproductive capacity, high stress resistance and the like are kept, the growth speed and the meat production rate of the bred pigs are improved through the advantages of the foreign boars, and the requirements of current pig raising production for the fast-growing high-quality black pigs are met.

The application provides application of a genetic marker related to the age of laying onset in a STON2 gene in genetic breeding of laying hens, belongs to the field of animal genetic breeding and biotechnology, and the genetic marker related to the age of laying onset in the STON2 gene is AFE_tag5, which corresponds to sequence information of the 5th chromosome physical position of the bGa lGa l 1.mat.broi ler.GRCg7b version published in a chicken reference genome of NCBI, is encoded as rs315655133 in Ensemb l, belongs to a synonymous mutation in an exon region of a gene STON2, and the base is T or C. The genetic marker AFE_tag5 is helpful for exploration and utilization of local chicken gene resources, is applied to genetic breeding of the chicken, and is favorable for improving the age of laying onset and obtaining a laying hen breed with excellent performance in uniformity of the age of laying onset.

The invention belongs to the technical field of biology, and particularly relates to a method for improving in-vitro mature oocyte telomere length and maturation quality and application of the in-vitro mature oocyte telomere length and the maturation quality. And daphnetin. According to the preparation disclosed by the invention, at least one of the Sema3 type protein and the daphnetin is added, so that the telomere length of an oocyte and an in-vitro embryo is effectively maintained, repaired or prolonged, the maturation quality of the in-vitro oocyte and the development potential and development efficiency of the in-vitro embryo are further remarkably improved, and further, the development potential and development efficiency of the in-vitro embryo are further improved. The application provides a more effective new strategy for in-vitro embryo production of livestock, animal genetic improvement, assisted reproduction technology and the like, and the application prospect is wide.

This invention relates to the fields of molecular markers and animal genetic breeding technology, and particularly to a SNP marker affecting the total flavor nucleotide content in pigs and its application. Based on three experimental pig populations—purebred American Landrace, purebred American Large White, and purebred American Duroc—this invention employs whole-genome resequencing and GWAS analysis to study and identify nine SNP markers associated with total flavor nucleotides. By optimizing the dominant alleles of these SNPs, the frequency of dominant alleles can be increased generation by generation, thereby increasing the total flavor nucleotide content, improving pork quality, accelerating the progress of pig genetic improvement, and effectively improving the economic benefits of pig breeding.

This invention belongs to the field of animal genetic engineering technology, specifically relating to a cashmere goat circRNA, an interference vector, and their application in regulating cashmere goat hair follicle development. The cashmere goat circRNA is circ-0000987; the base sequence of circ-0000987 is shown in SEQ ID NO.1, and it is used to regulate cashmere goat hair follicle development. This invention reveals for the first time the inhibitory effect of circ-0000987 on the proliferation of cashmere goat papillary cells. The results of this invention show that circ-0000987 plays a proliferative inhibitory role in cashmere goat papillary cells, inhibiting their activity by negatively regulating cell proliferation-related pathways; and participating in the transition of hair follicles from the anagen to catagen phase by inhibiting cell proliferation and promoting apoptosis.

This invention relates to the fields of gene editing and animal genetic breeding technology, and discloses a precise editing method for key genes regulating lipid metabolism in cattle and sheep. The method includes: constructing a comprehensive lipid metabolism network map integrating transcriptomic, proteomic, and metabolomic data; using a graph neural network to identify key node genes with high flow weights and their negative feedback pathways; simulating cascade reactions after gene perturbation to assess compensatory effects; screening for multi-gene combinations that do not induce significant compensation and can synergistically regulate fat deposition and muscle growth; designing expression vectors containing multiple guide RNA sequences to achieve simultaneous and precise editing; and obtaining edited individuals and validating the phenotype through embryonic or somatic cell nuclear transfer. This invention, through system-level multi-target synergistic editing, avoids metabolic imbalances, improves editing effectiveness, and promotes muscle development while reducing fat deposition, providing a safe and efficient technical path for precision breeding of cattle and sheep.