21 results about "Animal Genetics" patented technology

This invention discloses a molecular marker for superovulation in cattle using the EXOC4 gene and its application method, belonging to the field of animal genetic engineering technology. By conducting correlation analysis between EXOC4 gene polymorphism and the effect of superovulation in cattle, the single nucleotide polymorphism at a specific site of the EXOC4 gene and its accurate identification method were clarified, and the influence of this site's genetic polymorphism on the effect of superovulation in cattle was determined. This provides an important theoretical basis and application prospect for using it as a molecular marker for auxiliary selection of bovine reproductive performance and applying it to genetic improvement in actual production.

This invention relates to the fields of molecular markers and animal genetic breeding technology, specifically to a molecular marker located on chromosome 4 of pigs that is associated with body size and carcass traits, and its application. Using the F7 generation of a chimeric family as the research object, this invention employs whole-genome resequencing and GWAS analysis to study and identify the molecular markers located on chromosome 4 of pigs associated with body size and carcass traits. By optimizing the dominant alleles of these molecular markers, the frequency of dominant alleles can be increased generation by generation, enabling rapid and accurate selection of body size and / or carcass traits, accelerating the progress of pig genetic improvement, and thus effectively improving the economic benefits of pig breeding.

The application belongs to the technical field of animal genetic engineering, and particularly discloses a construction method of C1R gene humanized animal cells and animal models and application thereof. The construction method of the C1R gene humanized animal cells comprises the following steps: introducing a human C1R gene into non-human animal cells, so that the human C1R gene is expressed in the non-human animal cells to produce humanized C1R protein, and meanwhile, the expression of an endogenous C1ra gene in the non-human animal cells is reduced or eliminated. The application preferably adopts CRISPR / Cas9 gene editing technology to accurately insert a genomic sequence encoding a human C1R mature protein into a 2nd exon region of a mouse C1ra gene, while retaining a mouse source promoter, a 5' untranslated region (UTR) and a signal peptide sequence. A mouse cell or model capable of specifically interacting with an anti-human C1R antibody is successfully constructed.

The application relates to the field of animal genetic breeding technology, and discloses a local pig breed multi-node block chain collaborative breeding method. According to the method, key data abstracts, evaluation results, model versions and breeding decisions are chained and left marks in a window period through a permissioned consortium chain and a consensus verification mechanism, the source is clear, tamper-proof and auditable, the problem that data standards are different, difficult to trace and difficult to trust in traditional multi-subject collaboration is solved, in addition, an AI multi-factor breeding evaluation system can simultaneously process multiple traits such as meat quality, growth, reproduction, health resistance and environmental management factors, can depict the nonlinear correlation between complex traits, can improve the evaluation precision and stability of the comprehensive genetic value of local pig breeds, can reduce the risk of decision-making errors caused by single-point data or single-algorithm deviation, can realize cross-node alignment and joint use of dispersed data through a rolling window and a labeled archive, and can make up for the short board of insufficient samples of a single breeding unit.

The application provides a genetic marker associated with chicken intestinal length in a KCNIP4 gene and an application thereof, and belongs to the fields of animal genetics and breeding and biotechnology.The genetic marker comprises IL_tag1 or IL_tag2; the Ensembl number of the IL_tag1 is rs316532738, corresponds to the sequence of a positive strand of a chromosome No.4 of a chicken reference genome bGalGal1.mat.broiler.GRCg7b published by NCBI, is located in the 1st intron of a gene KCNIP4, and the base at the position is T or C; the Ensembl number of the IL_tag2 is rs316953671.The genetic marker is helpful to genetically improve the intestinal length of a laying hen, is applied to the genetic breeding of a chicken, and is favorable to improving intestinal traits and obtaining a laying hen variety with better nutrient absorption.

This invention relates to the fields of gene editing and animal genetic breeding technology, and discloses a precise editing method for key genes regulating lipid metabolism in cattle and sheep. The method includes: constructing a comprehensive lipid metabolism network map integrating transcriptomic, proteomic, and metabolomic data; using a graph neural network to identify key node genes with high flow weights and their negative feedback pathways; simulating cascade reactions after gene perturbation to assess compensatory effects; screening for multi-gene combinations that do not induce significant compensation and can synergistically regulate fat deposition and muscle growth; designing expression vectors containing multiple guide RNA sequences to achieve simultaneous and precise editing; and obtaining edited individuals and validating the phenotype through embryonic or somatic cell nuclear transfer. This invention, through system-level multi-target synergistic editing, avoids metabolic imbalances, improves editing effectiveness, and promotes muscle development while reducing fat deposition, providing a safe and efficient technical path for precision breeding of cattle and sheep.

This invention relates to the fields of animal genetics and breeding and biotechnology, and particularly to an overexpression method. Rlim Recombinant adeno-associated virus (AAV) vector, recombinant AAV, and its application in increasing milk production in animals. This invention utilizes a recombinant AAV vector to specifically overexpress a gene in mammary tissue. Rlim This study aims to provide a highly efficient, safe, and targeted approach to enhance lactation performance, thereby filling the technological gap in the application of this gene in lactation regulation and offering novel strategies and pathways for improving livestock production traits and potential interventions for related human health issues.

This invention relates to the field of animal genetic engineering technology, providing a molecular marker for the ATP5PO gene associated with bovine sperm motility and its application. The ATP5PO gene molecular marker is located at position 203 of the bovine ATP5PO gene fragment, where an A-G base mutation occurs, resulting in a single nucleotide polymorphism (SNP). The nucleotide sequence of the bovine ATP5PO gene fragment is shown in SEQ ID NO:1. Based on this ATP5PO gene molecular marker, genotype can be determined through sequencing, and the genotype can be associated with normal and thawed bovine sperm motility. This invention utilizes a specific primer set to obtain the bovine ATP5PO gene fragment associated with normal and thawed bovine sperm motility, and uses specific SNP sites within this fragment as molecular markers, providing a theoretical basis and practical application for marker-assisted selection in cattle.

The application belongs to the field of animal genetic engineering and molecular biotechnology, and particularly relates to FDXR The application of the expression activator of the gene in the preparation of a product for resisting porcine epidemic diarrhea virus infection. FDXR The nucleotide sequence of the gene is shown as SEQ ID NO. 1. The research result of the application shows that the expression level of the gene will affect the replication level of PEDV. FDXR When the expression of the gene in a host cell is inhibited, the replication of PEDV can be significantly promoted and the titer of the virus is increased. FDXR When the FDXR is overexpressed in the host cell, the replication of PEDV can be significantly inhibited and the titer of the virus is decreased. FDXR The experimental result not only shows that the gene can be used as a new target for the development of an antiviral drug for PEDV, but also provides a tool and material for the research on the molecular mechanism of the gene in the regulation of the replication of a pathogenic microorganism in a cell. FDXR The application belongs to the field of animal genetic engineering and molecular biotechnology, and particularly relates to

The application belongs to the technical field of animal genetic engineering, and provides a FETUB gene molecular marker related to the sperm vigor trait of a cow and application thereof, wherein the FETUB gene molecular marker is an SNP site on a FETUB gene fragment of the cow as shown in the sequence table SEQ ID NO:1, specifically, the 315th site of the FETUB gene fragment, the site has an A-G base mutation, resulting in the generation of a single nucleotide polymorphism of the gene. Based on the FETUB gene molecular marker, the genotype is determined by sequence determination, the genotype is associated with the normal and post-thawing vigor traits of the sperm of the cow, and the normal and post-thawing vigor of the sperm of individuals with different genotypes has a significant difference. The FETUB gene molecular marker provided by the application provides a theoretical basis and specific application for marker-assisted selection of the cow.

The present application relates to the application of Hu sheep FGF1 gene and its coded protein, and belongs to the field of biotechnology. The application of Hu sheep FGF1 gene and its coded protein in regulating the level of oxidative stress, cell apoptosis or cell proliferation of ovary granulosa cells. The present application first systematically identifies the CDS and protein sequence of sheep FGF1 gene, and analyzes its physical and chemical properties, post-translational modification sites, secondary and tertiary structures and systematic evolution relationship, which provides a molecular basis for revealing the role of the gene in oxidative stress and cell fate regulation. In addition, the disclosed sequence information and variation data are helpful for evaluating population genetic diversity, and have important scientific value for the protection, development and evolution research of animal genetic resources.

This invention discloses the application of the USP28 gene in the proliferation of porcine muscle stem cells, belonging to the field of animal genetic engineering. Resequencing data shows that this gene is selected in pig breeds with high meat production traits. Functional verification indicates that knocking down this gene in primary porcine muscle stem cells significantly reduces the mRNA expression level of the proliferation marker gene and significantly decreases the number of EdU-labeled proliferative positive cells. This invention clarifies the proliferation-promoting function of the USP28 gene, which can be used as a molecular marker or target gene for pork quality improvement and new breed breeding, providing an effective technical means to solve the problems of insufficient pork yield and genetic improvement of quality traits.

The application discloses a CDK5RAP3 gene SNP molecular marker related to yellow-feathered broiler feed conversion rate and application thereof, and belongs to the field of animal genetics and breeding and molecular biology. The SNP molecular marker polymorphism is A / G, is located at the 369307th site of chicken chromosome 27, and has an RS number of rs738324012. The application provides a SNP molecular marker related to yellow-feathered broiler feed conversion rate in a CDK5RAP3 gene on chicken chromosome 27, the marker indicates that there is a significant difference in feed conversion rate between yellow-feathered broilers with different genotypes, and the marker can be used for efficiently screening individuals or parent yellow-feathered broilers with excellent low feed conversion rate properties; when the marker is applied to yellow-feathered broiler breeding, yellow-feathered broilers with low feed conversion rate can be quickly bred, feed consumption in the production process can be effectively reduced, and economic benefits and competitiveness of enterprises can be improved.

The application belongs to the technical field of animal genetics and breeding, and provides a genomic selection analysis method considering trait local genetic correlation significance, comprising a phenotype data processing module, a genotype data processing module and a genomic selection module; the application performs quality control on the phenotype data; performs quality control, sequence alignment and variation detection on the genotype data; performs whole genome correlation analysis to generate summary statistical data; identifies whole genome local genetic correlation, tests the significance of LGC, and realizes double-trait genomic selection analysis with segment weighting; and the application can effectively improve the genetic evaluation accuracy of complex traits, especially low heritability traits, and provides an efficient and robust genomic selection tool for livestock breeding.

This invention discloses a SNP molecular genetic marker for the WNT6 gene related to feed conversion ratio in chickens and its application, belonging to the fields of animal genetics and breeding and molecular biology. The aforementioned SNP molecular genetic marker is located at locus 22401119 of the rs733687399 sequence on chicken chromosome 7, and the locus is a T>G mutation. On the one hand, this invention provides the SNP molecular genetic marker chr7-22401119, which is significantly correlated with the feed conversion ratio trait in yellow-feathered broilers. It can be applied to the breeding of yellow-feathered broilers, allowing for the selection of yellow-feathered broilers with low feed conversion ratios, effectively reducing feed consumption during production and improving the economic benefits and competitiveness of enterprises. On the other hand, this invention also provides primers and a kit for identifying the SNP molecular genetic marker chr7-22401119, which can be used for efficient screening of individuals or parents with excellent feed conversion ratio traits in yellow-feathered broilers.

The application discloses a TMEM165 gene SNP molecular marker related to yellow-feathered broiler feed conversion rate and application thereof, and belongs to the technical field of animal genetics and breeding and molecular biology. The SNP molecular marker is located at the 64634301th base of a sequence of chicken chromosome 4 rs312546095, and the polymorphism is G / A. The application provides a SNP molecular marker of a TMEM165 gene related to the yellow-feathered broiler feed conversion rate. The polymorphism of the SNP molecular marker is significantly related to the yellow-feathered broiler feed conversion rate. When the SNP molecular marker is applied to the breeding of yellow-feathered broilers, yellow-feathered broilers with low feed conversion rate can be quickly selected and reserved. The SNP molecular marker can be applied to efficiently screen yellow-feathered hens with low feed conversion rate and excellent shape, effectively reduce the feed consumption in the production process, and improve the economic benefits and competitiveness of enterprises.

This invention belongs to the field of molecular biology and relates to animal genetics and breeding. It specifically provides genes, primers, applications, and methods for identifying the developmental stages of the clitellum in *Pheretima aspergillum*. Addressing the shortcomings of existing artificial breeding methods for *Pheretima aspergillum*, which rely heavily on visual observation of the clitellum and suffer from significant physiological lag, this invention is the first to discover and screen specific molecular marker genes closely related to the initiation and maturity stages of clitellum differentiation in *Pheretima aspergillum*, and provides highly specific amplification primer sets and detection methods. This invention achieves very early molecular early warning and objective quantitative identification of clitellum development in *Pheretima aspergillum*, providing molecular standards for the quantitative control of nutritional intervention and mating / harvesting timing in artificial breeding. This technology helps to break through the bottleneck of large-scale *Pheretima aspergillum* farming, reduce dependence on wild resources, and is of great significance for protecting ecological red lines and the safety of the traditional Chinese medicine industry.

The application belongs to the technical field of animal genetic engineering, and provides a DHX36 gene molecular marker related to the sperm vigor trait of a cow and application thereof, wherein the DHX36 gene molecular marker is a C-G base mutation at the 231st site of a bovine DHX36 gene fragment shown in the sequence table SEQ ID NO:1. Based on the DHX36 gene molecular marker, the genotype is determined by sequence determination, and the genotype is associated with the normal and post-thawing sperm vigor trait of the cow. The analysis result shows that the normal and post-thawing sperm vigor of individuals with different genotypes is significantly different. The application uses specific primers to obtain a bovine DHX36 gene fragment related to the normal and post-thawing sperm vigor trait of the cow, and uses a specific SNP site in the fragment as a molecular marker, thereby providing a theoretical basis and specific application for marker-assisted selection of the cow.

The present application relates to the field of animal genetic breeding and intelligent animal husbandry, in particular to a crossbreeding improvement method for Simmental cattle, comprising: obtaining whole genome sequence data, phenotypic data and target area environmental climate time series data of a target population; inputting the three types of data into a pre-trained multi-modal dynamic model, extracting features and quantifying the output of recessive genetic load index; judging the relationship between the index and the preset safety threshold; when the index is lower than the safety threshold, generating a first selection scheme based on a pre-constructed deep reinforcement learning model, with the goal of maximizing the intergenerational genetic progress rate of production performance; when the index is higher than or equal to the safety threshold, generating a second selection scheme with the goal of maximizing genetic distance; the present application realizes the dynamic balance between short-term improvement efficiency and long-term breeding safety.

This invention discloses a SNP molecular genetic marker for the NFE2L1 gene related to feed conversion ratio in chickens and its application, belonging to the fields of animal genetics and breeding and molecular biology. The aforementioned SNP molecular genetic marker is located at locus 6493284 on chromosome 27, with the sequence number rs731344472, and is a G>T mutation. The SNP molecular genetic marker chr27-6493284 provided by this invention has been experimentally verified to be significantly associated with the feed conversion ratio trait in yellow-feathered broilers. It can be applied to the breeding of yellow-feathered broilers with low feed conversion ratios, effectively reducing feed consumption during production and improving the economic benefits and competitiveness of enterprises. Primers and kits for SNP molecular genetic marker identification can be used for efficient screening of individuals or parents with excellent feed conversion ratio traits in yellow-feathered broilers.

This invention discloses a SNP molecular marker associated with the age at which Large White pigs reach 120 kg body weight and its application, belonging to the fields of biotechnology and animal genetics and breeding technology. The marker is located on chromosome 13 of the pig reference genome Sscrofa11.1, at chr13:206033545 (rs344256714), within the PDE9A gene coding region, with an allele of T / G, located at position 300 from the 5′ end of the nucleotide sequence shown in SEQ ID NO.1. Under the same feeding conditions, individuals with the TG genotype have a significantly lower D120 than those with the TT genotype, exhibiting a heterozygous overdominance effect. This invention, by detecting the genotype at this locus, enables early molecular prediction, hierarchical management, and marker-assisted selection of Large White pig growth rate. Based on the selection index and optimal contribution selection strategy constructed using this marker, genetic improvement of growth traits in Large White pig populations can be achieved.