45 results about "Cortical neuron" patented technology

A Switching Kalman Filter Model for the real-time inference of hand kinematics from a population of motor cortical neurons. Firing rates are modeled as a Gaussian mixture where the mean of each Gaussian component is a linear function of hand kinematics. A “hidden state” models the probability of each mixture component and evolves over time in a Markov chain. Gaussian mixture models and Expectation Maximization (EM) techniques are extended for automatic spike sorting. Good initialization of EM is achieved via spectral clustering. To account for noise, the mixture model is extended to include a uniform outlier process. A greedy optimization algorithm that selects models with different numbers of neurons according to their decoding accuracy is used to automatically determine the number of neurons recorded per electrode. Closed loop neural control of external events are demonstrated using neural control of a computer curser.

A nanostructured molecular delivery vehicle comprising magnetic materials and configured to receive passenger biomolecules. The application of a an appropriate magnetic field having a gradient orients and drives the vehicle into a biological target, which may comprise cells, cell masses, tissue slices, tissues, etc. Under the control of the magnetic field, these vehicles can penetrate cell membranes. Then, the biomolecules carried by the vehicle can be released into the cells to perform their functions. Using this “nanospearing” technique, unprecendented high transfection efficiency has been achieved in several difficult-to-transfect cells. These include, but are not limited to, Bal 17 cells, ex vivo B cells, primary cultured cortical neurons, etc. This method advances the state of the art, providing an improved technique for the introduction of exogenous molecules to cells, with the clinical applications including, but not being limited to, drug delivery, gene therapy, vaccination, etc.

The invention discloses application of artemisinin and derivatives thereof in preparing medicaments for preventing and treating neurological diseases. In the application, the nerve cell line of rat adrenal pheochromocytoma PC12, retinal nerve cell line RGC-5 and primary cortical neuron are on behalf of experiment objects, sodium nitroprusside and hydrogen peroxide are used to simulate and induce oxidative stress, cell apoptosis and other cell injuries of nerve cells. Researches discover that the artemisinin has a protective effect on sodium nitroprusside and hydrogen peroxide induced cell injury, the protective effect mechanism of the artemisinin and derivatives thereof is primarily discussed, and results indicate that the artemisinin and derivatives thereof can be used as nerve protection medicaments for preventing and treating various neurological diseases, such as various neurodegenerative diseases, acute and chronic neurodegenerative diseases, neurological eye disease and the those neurological diseases mainly related to oxidative stress injuries and cell apoptosis. The application brings a new direction to treatment and prevention of neurological diseases.

The invention discloses new pharmaceutical uses of 7, 4'- di(mono succinate) oxyethoxypropyl daidzein. Pharmacological activity research is carried out to DZ5 by an experimental model for mice under atmospheric hypoxia tolerance, an experimental model for mice acute cerebral ischemia, an experimental model for bilateral common carotid arteries and vagus nerve ligation, an experimental model for mice KCN poisoning, an experimental model for mice NaNO2 poisoning and experimental model for learning and remembering, an action model for rat cortical neuron damage caused by anoxia of DZ5, an effect model for rat myocardial ischemia caused by hypophysin, an experimental model for vitro anti platelet aggregation, an experimental model for vivo anti platelet aggregation, and an effect model for arterial thrombosis in rabbits. Proved by the result, DZ5 has the functions of anti-cerebral ischemia and anoxia, anti-myocardial ischemia and anoxia, antagonistic memory disorders, anti-platelet aggregation and thrombosis, and protection of rat cortical neuron damage caused by anoxia. DZ5 can be mixed with a pharmacologically acceptable carrier or excipient to prepare medicine compositions.

The invention relates to a neuron in-vitro culture method for synaptic plasticity research on a visual cortex neuron. The method is characterized by comprising the following steps: (1) carrying out pretreatment on cerebral tissues of fetal rats, separating visual cortex tissues and carrying out proteinase digestion and centrifugation; (2) adding an inoculation medium to terminate digestion; (3) blowing a single-cell suspension, sieving and counting; and (4) inoculating with the inoculation medium of which the density is 2.5*10<4> / cm<2> to 5*10<4> / cm<2>, carrying out adherent culture in a full liquid-exchange manner, and after cell attachment, carrying out subsequent culture in a half liquid-exchange manner. The invention further provides a visual cortex neuron cell line of rats, which is cultured by the neuron in-vitro culture method, and an application of the visual cortex neuron cell line in synaptic plasticity research on the visual cortex neuron. The culture method is efficient and simple; the neuron is high in purity, and good in state, and has a structural basis of neuronal synaptic plasticity; the functional state of the neuronal synaptic plasticity can be reflected; and the culture method can be applied to research on the synaptic plasticity of visual cortex of rats.

The invention provides application of mesenchymal stem cell derived extracellular vesicles to cerebral ischemia-reperfusion injury. Research of the invention finds and verifies that extracellular vesicles (MSCs-EVs) derived from mesenchymal stem cells are capable of treating disordered brain functions and taking a neuroprotective effect into play through neurogenesis and angiogenesis after stroke.Research results show that within 24 hours and 48 hours after MCAO (middle cerebral artery occlusion), MSCs-EVs are capable of remarkably reliving neurologic impairment of rats, reducing the volume of cerebral infarction and brain water contents, relieving pathological injury of brain tissue cortexes, weakening cortex neuronal apoptosis, remarkably increasing p-AMPK (protein kinase), and reducingp-JAK2, p-STAT3 and p-NF-kB. Due to the application, essential support is provided for potential treatment strategies of MSCs-EVs as MCAO treatment.

A nanostructured molecular delivery vehicle comprising magnetic materials and configured to receive passenger biomolecules. The application of a an appropriate magnetic field having a gradient orients and drives the vehicle into a biological target, which may comprise cells, cell masses, tissue slices, tissues, etc. Under the control of the magnetic field, these vehicles can penetrate cell membranes. Then, the biomolecules carried by the vehicle can be released into the cells to perform their functions. Using this “nanospearing” technique, unprecendented high transfection efficiency has been achieved in several difficult-to-transfect cells. These include, but are not limited to, Bal 17 cells, ex vivo B cells, primary cultured cortical neurons, etc. This method advances the state of the art, providing an improved technique for the introduction of exogenous molecules to cells, with the clinical applications including, but not being limited to, drug delivery, gene therapy, vaccination, etc.

The invention discloses applications of dendrobium nobile lindl alkaloids (DNLA) in medicines prepared for treating ischemic brain damage and a product thereof. The DNLA is lipophilic total alkaloids composition which is extracted from the dendrobium nobile lindl. Compared with the prior art, the invention aims at the nosogenesis of stroke, studies the protection function of the DNLA on oxygen-glucose deprivation injury model of primary cortical neurons and acute cerebral ischemia model of rats, analyzes the mechanism, further makes clear the pharmacological action and mechanism action of the DNLA for treating cerebral ischemia, and provides pharmacological basis for the development and applications of the dendrobium nobile lindl medical material.

The invention belongs to the technical field of image feature extraction and target recognition. The invention discloses an image feature extraction and target recognition method and system, a storagemedium and a terminal, the method comprises the steps of: establishing a computer model suitable for human primary visual cortex neuronal cells, establishing a recognition response mechanism of a visual abdominal side system, and performing image feature extraction, sample training and object recognition on the visual abdominal side system. Results show that compared with a traditional method, the method effectively improves the recognition of the image features, and the image features extracted in a complex background scene have good generalization ability. On the basis, the training samplecan be effectively identified. Results show that recognition of the edge, direction and contour of a training sample based on a model of a visual neural mechanism shows the advantages of a biologicalvisual mechanism in the aspect of object recognition.

The invention relates to magnolia odoratissma alkyl diol which promotes regeneration of a nerve cell system and nerve cell axons, can promote increase of low-concentration induced PC-12 cell differentiation amount, obvious increased of the cell axon amount, obvious increase of axon length, obvious increase of neuroma mother cell N1E-115 axon length and prolonging and regeneration of fetal rat primary cortical neuron axons.

The present invention demonstrates the therapeutic use of ketone esters for seizure disorders, Alzheimer's disease and malignant brain cancer, which are associated with metabolic dysregulation. The administration of ketone esters resulted therapeutic ketosis and neuroprotection against seizures resulting from CNS oxygen toxicity. Supplemental ketones were also found to reduce superoxide production in cultured cortex neurons exposed to hyperbaric oxygen and Aβ-42, and to decrease proliferation and viability in U87 glioma cells. These observations support the therapeutic effect of ketones for seizure disorders, Alzheimer's disease and malignant brain cancer. The ketone esters may be derived from acetoacetate and can include R,S-1,3-butanediol acetoacetate monoester, R,S-1,3-butanediol acetoacetate diester, or a combination of the two.

The invention discloses benzotetrazole derivatives. The benzotetrazole derivatives have a structure represented by a general formula I or a general formula I'. In the general formula I or the generalformula I', R1 is a C3-C5 alkyl chain or a halogenated alkyl chain, R2 is one or a plurality of elements selected from H, Cl, F and Br or a combination of two or a plurality of elements selected fromH, Cl, F and Br, R3 is H, F or CH3, and R4 is a C1-C2 alkyl chain. The invention also discloses optical isomers of the derivatives, or pharmaceutically acceptable salts or solvates of the derivatives,or pharmaceutically acceptable salts or solvates of the optical isomers. The invention further discloses compositions containing the benzotetrazole derivatives and application of the compositions inpreparation of medicines for preventing and resisting cerebral apoplexy. Repeated experiments prove that the derivatives provided by the invention shows strong protective activity on OGD / R molded mouse primary cortical neuronal cells. Therefore, the derivatives disclosed by the invention can be applied as neuroprotective agents to medicines for treating and preventing cerebral apoplexy.

Artificial expression constructs for selectively modulating gene expression in selected central nervous system cell types are described. The artificial expression constructs can be used to selectively express synthetic genes or modify gene expression in excitatory cortical neurons, such as primarily within cortical layers 2 / 3, 4, 5, and 6 and including those with extratelencephalic (ET) projections, intratelencephalic (IT) projections, and pyramidal tract (PT) projections, among others.

The invention discloses an NDUFS2 gene heterozygous editing pig as well as a construction method and application thereof, and relates to the field of animal gene engineering and disease model construction. The eighth exon of the NDUFS2 gene of the pig is deleted or mutated, and presents an NDUFS2 heterozygous genotype; in the newborn period, the number of neurons of the middle cerebral cortical layer and the number of dopaminergic neurons are obviously reduced, the number of neurons of the adult cortical layer is continuously reduced along with mitochondrial swelling and crest fracture, and after MPTP treatment, the number of neurons of the adult cortical layer is disordered in motion trail, and the body tremor index is increased. The pig model not only can be used for revealing the action mechanism of the NDUFS2 defect in neurodegenerative diseases (such as Parkinson's disease), but also can be used as an important tool for screening new drugs, evaluating treatment means and researching mitochondrial diseases.

The invention discloses a binocular modulation difference-based visual stabilization method, and particularly relates to the technical field of biomedical engineering, and the method comprises the following steps: S1, binocular neural signal conduction delay data acquisition, S2, visual stability adaptive time difference threshold setting, S3, near-infrared light parameter and conduction velocity association relationship establishment, S4, binocular near-infrared light parameter combination screening, and S5, visual stability adaptive time difference threshold setting. And S5, calibrating double-path driving delay parameters. According to the method, a time difference threshold interval is set in combination with a visual phase difference physiological adaptation range, the incidence relation between the near-infrared light parameters and the nerve conduction velocity is established, parameter setting is deeply fused through binocular nerve conduction delay data and cortical neuron response time sequence data, the adaptability of the parameters and the nerve physiological state is enhanced, and the accuracy of the nerve physiological state is improved. The collaborative accuracy of near-infrared light modulation parameters of the two eyes is improved, it is ensured that the time sequence of the two-way driving signals is accurately matched with a nerve regulation target needed by visual stability, and the problem that the visual regulation effect is poor due to parameter setting deviation is solved.

The invention discloses a three-dimensional combined micro-channel for inducing orientated growth of primary cortical neuron axons. The three-dimensional combined micro-channel comprises a soft photoetching supporting channel and a two-photon photoetching axon growth bracket, a first interaction interface and a second interaction interface are formed in the soft lithography supporting flow channel, and a first cell chamber and a second cell chamber are arranged in the soft lithography supporting flow channel; the first cell chamber is communicated with the first interaction interface, and the second cell chamber is communicated with the second interaction interface; the two-photon photoetching axon growth support is embedded in the soft photoetching supporting flow channel, the two-photon photoetching axon growth support comprises a growth cavity penetrating through the two-photon photoetching axon growth support, the first end of the growth cavity is communicated with the first interaction interface, and the second end of the growth cavity is communicated with the second interaction interface; the growth cavity comprises at least one ascending section, and the ascending section is formed by changing the growth cavity in the Z-axis direction. The problem that three-dimensional directional growth of nerve axons under the directional flowing condition is difficult to achieve through a flow channel in the prior art is solved.

The invention discloses application of cholest-3,6-dione, deuterated substances thereof or pharmaceutically acceptable salts thereof to preparation of drugs used for treating the neuronal damage of patients. According to the invention, it is proved that cholest-3,6-dione can alleviate the oxidative stress damage of hippocampal neurons induced by glutamate, mitigate the apoptosis of cerebellar granule neurons caused by potassium deprivation, and abate the neuroexcitotoxic injury of cortical neurons induced by glutamate, so cholest-3,6-dione can be applied to treatment of neuronal damage.

The invention belongs to the technical field of biological medicine, and relates to a recombinant NMDA receptor antibody and application thereof. The invention relates to a recombinant NMDA receptor antibody Tat-DiN2B and application of the recombinant NMDA receptor antibody Tat-DiN2B in preparation of a medicine for inhibiting neurological function defect. The nerve injury which can be inhibited by the Tat-DiN2B comprises cortical neuron excitatory toxicity injury, acute hippocampal brain slice excitatory toxicity injury and cerebral ischemia injury. Meanwhile, the Tat-DiN2B can also be used for improving the dyskinesia and anxiety level after cerebral ischemia. The Tat-DiN2B disclosed by the invention is constructed by taking a gene synthesized by Tat and DiN2B as a template through the steps of PCR (Polymerase Chain Reaction) amplification, enzyme digestion, transformation, transfection, expression, purification and the like, and a DNA (Deoxyribonucleic Acid) sequence of the Tat-DiN2B is as shown in SEQ ID No.1.

The invention discloses an induced pluripotent stem cell carrying angel syndrome specific genetic information and application of the induced pluripotent stem cell, and relates to the technical field of stem cells. The induced pluripotent stem cell carries angel syndrome specific genetic information and can be further induced into excitatory cortical neuronal cells, and a good cell model is provided for exploration of pathogenesis of neurodevelopment disorder diseases, drug screening and the like.

Methods for generating mature neurons differentiated from pluripotent stem cells, such as to facilitate the study of late-onset neurodegenerative disease, are disclosed. The methods include overexpressing the splicing factor muscleblind like splicing regulator 2 (Mbnl2) in cortical neurons. In some aspects, the method further comprises overexpressing RNA binding fox-1 homolog 1 (Rbfox1) and / or reducing expression of polypyrimidine tract binding proteins PTBP1 / 2. The methods also include accelerated derivation of mature neuron and generation of a tau pathology model. Also disclosed are constructs and compositions for accelerated derivation of mature neuron and / or generation of a tau pathology model.

The invention discloses a tetrazole derivative with a structure as shown in a general formula I. The invention also discloses a composition containing the tetrazole derivative and application of the composition in preparation of drugs for preventing and resisting cerebral apoplexy. Through multiple experiments, the inventor of the invention proves that the compound has an obvious inhibition effect on ADP-induced platelet aggregation activity, and has an outstanding damage protection effect on neuron-like cells induced and differentiated by OGD / R fetal mouse primary cortical neuronal cells and neuroblastoma cells (N2A); for a focal cerebral ischemia animal model constructed by transient middle cerebral artery occlusion through a suture method, the compound has a good anti-cerebral apoplexy drug effect and good rat oral pharmacokinetic property. Therefore, the compound disclosed by the invention can be used as a neuroprotective agent to be applied to medicines for treating and preventing cerebral apoplexy.

The invention relates to a contour detection method based on the synergistic effect of multi-level inhibition zones of visual pathways. The present invention first constructs a direction-sensitive outer geniculate neuron array, records the contour direction selected by the outer geniculate body, and calculates the response of the outer geniculate neuron; takes the distance as a factor for the visual input of the classic receptive field and inhibitory area of ​​the outer geniculate neuron Carry out quantitative processing, take the difference between the two as the effective response of the inhibition area, and obtain the synergy parameters through dynamic rectification and power exponent normalization; obtain the visual input of the primary visual cortex through the local window weighted fusion of the response of the outer geniculate neurons, and detect Accurate contour response; calculate the primary visual cortex neuron inhibitory area response to obtain the final contour response. The invention considers the step-by-step refined detection mechanism when neurons at different levels perceive directions in the visual pathway, and simultaneously simulates the synergistic effect of inhibition areas between different levels, which can effectively improve the contour detection performance of natural images.

The invention discloses an application of interaction of cFKBP8-Hsp60 in depression by adjusting mitochondria. Belongs to the technical field of molecular biology and medicine, the interaction between cFKBP8 and Hsp60 is ensured, the key amino acid residue of the interaction between cFKBP8 and Hsp60 is confirmed to be the 13th arginine (cFKBP8 ARG13) of cFKBP8, and the cFKBP8 is overexpressed in SH-SY5Y cells and mouse leading edge cortical neurons by further constructing an expression vector; the results show that the mitochondrial structure and function are damaged, and the mouse shows stress susceptibility, however, the mutation of cFKBP8 ARG13 into alanine alleviates mitochondrial damage and the stress susceptibility of the mouse. Therefore, based on a molecular mechanism of adjusting mitochondria by a cFKBP8-Hsp60 axis, a new thought and a treatment target are provided for the treatment of depression.

The invention relates to a method for producing hiPSCs derived brain-like tissue. The method comprises the following steps of (1) preparing a microcolumn array chip; and (2) inducing the brain-like tissue on the chip. Brain-like organs produced by the method have relevant characteristics of in vivo brain development on gene level and protein level. The method is simple, a tedious matrigel packaging step is removed, matrigel consumption is reduced, and only additional Dorsomorphine and A83-01 need to be added at the stage of forming an embryoid body. The brain-like organs induced to 30 days have expression of cortex nerve cells (TBR1 and CTIP2), expression of mature nerve cells (TUJ1), expression of front brain (PAX6 and FOXG1) and expression of afterbrain protein (PAX2 and ISL-1), and haveexpression of excitatory neuron (vGLUT1) subtypes and inhibitory neurons (GABA) subtypes.

The invention provides a Chinese medicinal composition for treating ischemia apoplexy, which is prepared from raw rehmannia, dendrobe, raw astragalus mongholicus, kudzuvine root, leech, hemlock parsley, earthworm, morinda officinalis and scutellaria baicalensis. The Chinese medicinal composition has the functions of remarkably reducing vascular permeability, reducing the symptom of neurologic impairment, reducing occurrence of cerebral infarction, reducing the total volume of infarction, infarction of cortex and infarction under cortex to varying degrees, and remarkably improving the survival rate of cortical neuron, and has good curative effect to ischemia apoplexy.

The present invention addresses the problem of providing a therapeutic agent for frontotemporal lobe degenerative disease (FTLD), a composition for treating FTLD, and a method for treating FTLD, which have not been developed so far. The inventor of the present invention finds that: a prefrontal lobe cerebral cortex neuron is differentiated and induced by an iPS cell derived from an FTLD patient; a therapeutic agent for frontotemporal lobe degenerative disease (FTLD), comprising a compound represented by formula (1-1), a compound represented by formula (2-1), or a compound represented by formula (3-1), a pharmaceutically acceptable salt thereof, or a solvate thereof, is administered to a prefrontal cerebral cortex neuron exhibiting a pathological state of FTLD. Therefore, the pathological state of FTLD of the forehead lobe type cerebral cortical neurons is improved.

The invention provides miR-25-3p as well as application and an application method thereof, and relates to the technical field of biomedicine, the miR-25-3p is used as a molecular intervention target, and overexpresses the miR-25-3p so as to promote growth of DGR neuron and cortical neuron axons. The miR-25-3p participates in peripheral nerve injury repair by adjusting the growth of DGR neuron axons. The miR-25-3p participates in central nervous system injury repair by adjusting cortical neuron axon growth. According to the invention, firstly, a small RNA, that is, miR-25-3p is found through screening, and is found can play an important role in nerve injury repair. The miR-25-3p mimic is transfected in the in-vitro DRG neuron, so that the growth of the DRG neuron subjected to primary culture and cortical neuron axons can be obviously promoted. The miR-25-3p can participate in peripheral nerve injury repair by adjusting DRG neuron axon growth, and can also participate in central nerve injury repair by adjusting cortical neuron axon growth. The important effect of miRNA in the nerve injury repair process can be better understood, and a new target spot is provided for treatment after nerve injury.

The invention discloses a culture medium and a method for obtaining an induced cortical-like neuron precursor cell cluster from iPSCs (induced pluripotent stem cells), and belongs to the technical field of biology. The culture medium comprises a neural stem cell induction liquid I and a neural stem cell induction liquid II, a single-layer adherent induction method is adopted, and iPSCs are efficiently differentiated into an induced cortical neuron precursor cell cluster through optimized inoculation density, matrix treatment and a staged induction process. The neural stem cells comprise neural stem cells, transition-state nerve cells and differentiated mature nerve cells (the cell type composition is highly similar to that of tissue-derived neural stem cells). The method overcomes the problems of complex induction process and high cell heterogeneity in a traditional embryoid body method, the induction efficiency can reach 100%, single cell sequencing analysis shows that all cells are nerve cells, the purity is 100%, and non-target cells are not detected. A uniform, stable and efficient cell tool is provided for brain development research, nervous system disease modeling and cell therapy.

The application discloses a tetrazole derivative, which has a structure shown in a general formula I. The application also discloses a composition containing the tetrazole derivative and application of the tetrazole derivative in preparation of a drug for preventing and resisting stroke. The inventors of the application have confirmed through multiple experiments that the compound has obvious inhibiting effect on ADP-induced platelet aggregation activity, outstanding damage protection effect on OGD / R fetal rat primary cortical neuron cells and neuron-like cells induced and differentiated from neuroblastoma cells (N2A), good anti-stroke efficacy on an animal model of focal cerebral ischemia constructed by a line plug method for temporarily blocking a middle cerebral artery and good rat oral pharmacokinetic properties. Therefore, the compound can be applied to a drug for treating and preventing stroke as a neuroprotective agent.

The invention belongs to the technical field of life science / medicine, and discloses a perception training device for auditory cortex plasticity based on painless training stimulation. The training device is located in a sound insulation shielding box, and comprises a controller, a sound generator, a loudspeaker, a feeding device, an infrared nose detector and an illuminating lamp. The invention further discloses application of the training device in induction and perception training of the auditory cortex plasticity. The device has the characteristics of non-invasion, broad spectrum and the like, improves the frequency selectivity of auditory cortex neurons, and has a wide application prospect.