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Application of extracellular vesicles derived from mesenchymal stem cells in cerebral ischemia-reperfusion injury

A technology with purpose and right and wrong, applied in the application field of extracellular vesicles in cerebral ischemia-reperfusion injury, can solve problems such as incompletely clear mechanisms, and achieve the effect of reducing neurological deficits, tissue damage, and apoptosis

Active Publication Date: 2022-01-25
SHANDONG UNIV QILU HOSPITAL
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Problems solved by technology

However, the inventors found that in the ischemia-reperfusion model, the mechanisms by which MSCs-EVs exert neuroprotective effects through AMPK and JAK2 / STAT3 / NF-κB signaling pathways are not fully understood

Method used

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  • Application of extracellular vesicles derived from mesenchymal stem cells in cerebral ischemia-reperfusion injury
  • Application of extracellular vesicles derived from mesenchymal stem cells in cerebral ischemia-reperfusion injury
  • Application of extracellular vesicles derived from mesenchymal stem cells in cerebral ischemia-reperfusion injury

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Experimental program
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Embodiment

[0076] 1. Experimental animals

[0077] Adult healthy male SPF grade SD rats weighing 280-320 g were selected for this study, and the experimental animals were purchased from Jinan Pengyue Experimental Animal Breeding Co., Ltd. (permission number: SCXK(LU)2019-0003). They were raised in the SPF grade animal breeding room of Shandong University Animal Center, with temperature (24±1°C), humidity of 45%-55%, and natural light / dark cycle (≈12h-12h). All rats had free access to food and water. Experiments were started after 7 days of adaptive feeding. All animal experimental procedures were performed in accordance with the guiding principles of the Chinese Care and Use Act and were approved by the Animal Ethics Committee of Shandong University Health Sciences.

[0078] 2. Establishment of MCAO model in rats

[0079] The modified Longa method was used to establish the rat MCAO (cerebral ischemia-reperfusion) model. The rats were fasted for 6 h before the operation to reduce comp...

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Abstract

The invention provides the application of extracellular vesicles derived from mesenchymal stem cells in cerebral ischemia-reperfusion injury. The present invention discovers and confirms that extracellular vesicles (MSCs-EVs) derived from mesenchymal stem cells can treat brain dysfunction and neuroprotection through neurogenesis and angiogenesis after stroke. The results of the study showed that MSCs-EVs could significantly alleviate the neurological deficit at 24h and 48h after MCAO in rats, reduce the volume of cerebral infarction and brain water content, improve the pathological damage of the cortex of the brain tissue, and attenuate the apoptosis of cortical neurons and significantly up-regulate p‑AMPK, while p‑JAK2, p‑STAT3 and p‑NF‑κB were downregulated. The present invention provides essential support for the use of MSCs‑EVs as a potential therapeutic strategy for MCAO treatment.

Description

technical field [0001] The invention belongs to the technical field of crude drugs and medicine, and in particular relates to the application of extracellular vesicles derived from mesenchymal stem cells in cerebral ischemia-reperfusion injury. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Cerebrovascular disease is the third leading cause of death in the world and one of the leading causes of death among Chinese residents. Its morbidity and mortality are increasing year by year, bringing heavy burden to society and economy. Ischemic stroke accounts for 75-80% of all strokes, and the incidence rate is significantly higher than that of hemorrhagic stroke. The pathologi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/28A61P9/10
CPCA61K35/28A61P9/10
Inventor 李刚王贞韩敏薛皓曹颖初锡丽辛丹清李婷婷柯鸿飞
Owner SHANDONG UNIV QILU HOSPITAL
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