39results about How to "Clear mechanism" patented technology

A composition for improving hyperglycemia of pets and a preparation method thereof belong to the field of health food for pets, are used for improving symptoms of hyperglycemia of pets, and are characterized by comprising 20-50 parts of sea cucumber oligopeptide, 10-30 parts of sea cucumber polysaccharide, 20-40 parts of a ginseng extract, 20-30 parts of an acanthopanax sessiliflorus extract, 10-20 parts of a white kidney bean extract and 10-20 parts of a poria cocos extract. According to the present invention, starting from the mechanism that glucose promotes insulin secretion, the pancreas islet beta cells are protected from excessive stimulation so as to have a rest, and the functional components directly protect and repair the pancreas, such that the normal function of the pancreas is recovered, and the continuous development of hyperglycemia to diabetes mellitus is blocked.

The invention belongs to the technical field of microorganisms, and relates to a bifidobacterium breve strain capable of relieving constipation, the strain is bifidobacterium breve Bb-PG-14, the classification name of the strain is Bifidobacterium breve, the preservation number is CGMCC No.31861, the preservation time is September 4, 2024, the preservation unit is China General Microbiological Culture Collection Center, the preservation number is CGMCC No.31861, the preservation number is CGMCC No.31861, the preservation number is CGMCC No.31861, the preservation number is CGMCC No.31861, the preservation number is CGMCC No.31861, and the preservation number is CGMCC No.31861. The address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain is separated and purified from excrement of healthy babies, has relatively strong acid resistance, alkali resistance, cholate resistance and artificial simulated gastrointestinal fluid tolerance, and has the characteristics of probiotics.

The invention belongs to the technical field of metal separation, and particularly relates to a deep eutectic solvent, a preparation method and application thereof, and a method for selectively extracting lithium from a lithium battery positive electrode material. According to the deep eutectic solvent provided by the invention, nearly 100% selective leaching of lithium is realized in an extremely short time, and Ni, Co and Mn are almost not leached, so that the bottleneck that the rate and the selectivity are difficult to obtain at the same time in a traditional method is broken through; and the process conditions are mild and efficient, are far superior to the traditional process and are more suitable for practical industrial application, and the energy consumption and material consumption are remarkably reduced.

The invention discloses a quantitative analysis method and system for overheat fault gas of an on-load tap-changer contact. The method comprises the following steps: constructing a multi-physical field coupling mathematical model of an on-load tap-changer, and defining contact resistance of a contact surface; solving the multi-physics field coupling model to obtain time domain and space domain temperature distribution of the surface of the on-load tap-changer contact, and determining the highest temperature and the volume of an overheat region; calculating the local gas production rate of each fault characteristic gas; carrying out integral calculation on local gas production rates of all fault characteristic gases in the hot area volume to obtain theoretical equilibrium concentration of each fault characteristic gas in oil, and constructing a mapping relation between an overheating parameter formed by the contact resistance and the highest temperature of the contact surface and the theoretical equilibrium concentration; the method aims at solving the problems that a traditional analysis method for the gas dissolved in the oil depends on experience criteria and is high in hysteresis quality, breaks through the bottleneck that the whole process of electricity-heat-fluidization cannot be simulated from the mechanism level, and achieves the crossing from qualitative diagnosis to quantitative prediction.

The application discloses application of delphinidin chloride glucoside or a pharmaceutically acceptable salt thereof in preparation of a medicine for preventing, alleviating or treating cardiorenal metabolic syndrome; the application adopts eNos ‑ / ‑ The drug efficacy is verified by using a db / db animal model, and 10 mg / kg of a low administration dose can significantly improve kidney excretion and filtration function under the condition of severe endothelial injury and metabolic disorder, and effectively reduce a plurality of core biochemical indexes such as urine albumin excretion rate (UACR), serum creatinine (SCr) and urea nitrogen (BUN). The application breaks through the deficiency that existing research is limited to cell experiments or light animal models, and confirms that the substance can effectively reverse myocardial cell hypertrophy, myocardial fibrosis, glomerular sclerosis and other organic pathological injuries from the organ level through cardiac ultrasound detection and histopathological section analysis. The application can provide a new single-drug treatment candidate for clinical treatment of late-stage severe diabetic complications, and is especially suitable for patients with double damage of heart and kidney function.

The invention provides a social disorder animal model construction method based on a basal lateral amygdaloid nucleus-screen nucleus neural circuit and application thereof, and belongs to the technical field of animal model construction. The method comprises the following steps: injecting a viral vector carrying a photosensitive protein gene or a photosuppressive protein gene into amygdaloid nucleus on the outer side of an animal basal to express neurons of cholecystokinin or neurons of CaMKII alpha; embedding an optical fiber above the screen-shaped core; first light stimulation is applied through an optical fiber to activate nerve projection from amygdaloid nucleus on the outer side of the substrate to the screen nucleus, and a social disorder animal model is obtained; or applying a second photostimulus to inhibit the projection, resulting in a social disorder relief animal model. According to the invention, specific photogenetic regulation and control of the BLACCK +-CLA neural circuit is realized for the first time, social behaviors can be accurately regulated and controlled without interfering with movement and anxiety levels, and a reliable experimental tool is provided for mechanism research and drug screening of social obstacles.

This invention discloses a Chlorella heptapeptide TGPLGSR with multi-target lipid-lowering activity, its preparation method, and its applications. The amino acid sequence of this heptapeptide is Thr-Gly-Pro-Leu-Gly-Ser-Arg. The preparation method includes: performing ultraviolet and electroporation combined mutagenesis on wild-type Chlorella proteoglycans to obtain a high-protein-content mutant algal strain; obtaining algal protein through fermentation and synergistic extraction and purification with alcohols; directionally digesting the protein with alkaline protease, followed by enriching the active peptide components through ultrafiltration and gel chromatography; and finally, screening the heptapeptide using liquid chromatography-mass spectrometry (LC-MS), computer-aided prediction, and molecular docking techniques. This heptapeptide exhibits potent inhibitory activity against pancreatic lipase and can dose-dependently reduce the accumulation of triglycerides and cholesterol in 3T3-L1 cells. Its mechanism involves multiple target pathways, including inhibition of MMP-9, activation of AMPK phosphorylation, and downregulation of PNLIP, and it can be used to develop lipid-lowering functional foods or health products.

The application discloses a kind of overheat fault gas quantitative analysis method and system of on-load tap-changer contact, the method of the present application includes constructing the multi-physics field coupling mathematical model of on-load tap-changer, define contact surface contact resistance;Solving multi-physics field coupling model obtains the time domain and spatial temperature distribution of on-load tap-changer contact surface, determine the highest temperature and the volume of overheated area;Calculate the local gas production rate of each fault characteristic gas;The local gas production rate of all fault characteristic gases in the volume of hot area is integrated to obtain the theoretical equilibrium concentration of each fault characteristic gas in oil, construct the mapping relationship between the overheating parameters of contact surface contact resistance and highest temperature and theoretical equilibrium concentration.The present application aims to solve the problem that the traditional oil dissolved gas analysis method relies on empirical criterion and has high hysteresis, break through the bottleneck that cannot simulate the whole process of "electric-thermal-flow-chemical" from mechanism level, realize the leap from qualitative diagnosis to quantitative prediction.

The invention relates to a ship stern near-hull flow field adjusting method, which comprises the following steps of: 1, calculating a flow field near a hull according to a ship type and a working condition, and selecting a position where a spoiler is arranged; according to numerical calculation and a model test, a streamline near a stern and a hull is obtained, and a spoiler is arranged at the downward flowing position of the streamline in front of a stern shaft; 2, evaluating the drag reduction effect corresponding to each attack angle by adopting numerical calculation; comparing the total resistance value R0 before the spoiler is additionally mounted with the total resistance Ri of the ship after the spoiler is additionally mounted to determine which attack angle is finally adopted; 3, evaluating the ship resistance reducing effect of the spoiler, and judging whether the scheme of the spoiler continues to be modified or not according to the effect; the anti-drag effect of the device is verified by means of numerical calculation or model test and the like. The spoiler is arranged on the ship body in front of the tail shaft, and water flow is adjusted from obliquely downward flow to horizontally backward flow, so that the resistance of the ship is reduced, and the propelling efficiency of the propeller is improved.

The application discloses low-bitter rice residue peptide micelles, a rice residue protein hydrolysate and a preparation method and application thereof, and relates to the technical field of protein processing. The preparation method comprises the following steps: performing step-by-step enzymolysis on rice residue protein to perform directional cutting on the hydrophilic region and the hydrophobic region of the rice residue protein, so as to obtain an amphiphilic polypeptide mixture; adjusting the pH and the temperature, so that the amphiphilic polypeptide mixture is self-assembled to form rice residue peptide micelles, and low-bitter rice residue peptide micelles are obtained. The application aims to solve the technical problems that the existing rice residue protein enzymolysis product has significant bitterness, and traditional debittering technologies have the problems of nutrient loss or the need for external addition. A new mechanism of realizing physical debittering by constructing a supramolecular micelle structure is innovatively proposed and verified. The rice residue protein peptides after enzymolysis are self-assembled to form stable nanometer micelles through step-by-step enzymolysis and directional cutting, so that the hydrophobic groups causing bitterness are selectively embedded in the core of the micelles, and the contact of the hydrophobic groups with taste receptors is physically blocked.

The invention relates to the technical field of cosmetics and preparations thereof, and discloses a heat-resistant liquid crystal emulsion system based on acetylated sodium hyaluronate as well as a preparation method and application thereof, the heat-resistant liquid crystal emulsion system comprises the following components in parts by weight: 1-6 parts of a liquid crystal emulsifier, 0.01-0.2 part of acetylated sodium hyaluronate, 5-60 parts of grease, 0.1-1 part of a thickening agent, 0.1-1 part of a preservative and the balance of water, totaling 100 parts. The preparation method comprises the following steps: respectively preheating the oil phase and the water phase according to the ratio, emulsifying under high-speed homogenization, and stirring and cooling at a controlled temperature. The core of the invention is that acetylated sodium hyaluronate is introduced into a liquid crystal system as a structural stabilizer, and the thermal stability of a liquid crystal structure is remarkably improved through the anchoring effect of the acetylated sodium hyaluronate and a liquid crystal layer; after the emulsion is stored at 40 DEG C for a long time, the liquid crystal texture is kept complete, and the emulsion has excellent long-acting moisturizing performance and can be used as an ideal carrier of high-end cosmetics and external medicine preparations.

This invention relates to the field of biomedical technology, and more particularly to the application of an isoflavone compound, specifically Corylifol A, in the preparation of drugs for the prevention and / or treatment of inflammatory diseases. When used to prepare drugs for the prevention and / or treatment of inflammatory diseases, this isoflavone compound can directly bind to the IκBα protein and specifically inhibit its phosphorylation, thereby blocking the phosphorylation and nuclear translocation of the NF-κB p65 subunit, ultimately inhibiting the overactivation of the NF-κB signaling pathway. Furthermore, the isoflavone compound can significantly reduce the mortality rate of lipopolysaccharide-induced septicemia in mice, inhibit the levels of inflammatory factors in serum and lung tissue, and effectively alleviate LPS-induced acute lung injury in mice. Simultaneously, when the isoflavone compound is controlled within an effective concentration range, it exhibits no significant cytotoxicity to mouse peritoneal macrophages and can significantly inhibit the production and release of lipopolysaccharide-induced inflammatory factors IL-1β, TNF-α, and IL-6.

The application discloses application of dimethoxyphenol and a biological preparation thereof in plant pathogenic nematode control, and aims to solve the problems of single function, unknown active ingredient, unclear mechanism and unstable quality of existing microbial source preparations. The application accurately identifies 2,3-dimethoxyphenol as an important nematicidal active substance through metabolomics, and realizes stable co-accumulation of 2,3-dimethoxyphenol and a growth-promoting factor, isopentenyladenine, by combining with a specific strain (pink helical polysporal mold NF-06 and bacillus velezensis YB-1652) co-culture process, so that technical effects of clear composition, synergistic disease-preventing and growth-promoting function, controllable quality and environment friendliness are achieved.

The invention belongs to the technical field of traditional Chinese medicine modernization and medicine development, and particularly relates to a uric acid reducing product based on folium apocyni veneti extract and application of the uric acid reducing product. The core active component of the product is the folium apocyni veneti extract. In-vivo experiments prove that the extract can remarkably reduce the blood uric acid level and xanthine oxidase (XO) activity of a mouse with hyperuricemia. Six active ingredients entering blood are identified through in-vivo metabolism research. The direct interaction between the main active component and the target protein XO is verified from the molecular level through molecular docking, surface plasmon resonance (SPR) and isothermal titration calorimetry (ITC) technologies, and the uric acid reducing action mechanism of the compound is completely clarified. The invention provides a scientific basis and a high-quality product for developing a new uric acid-reducing drug effect of the folium apocyni veneti.

The invention belongs to the field of food, and particularly relates to yoghourt capable of reducing blood sugar and a preparation method of the yoghourt. The preparation method comprises the following steps: adding mulberry leaf powder and / or a mulberry leaf water extract, mulberry powder, mogroside and radix puerariae powder into milk, uniformly stirring, and adding yoghourt zymophyte powder; according to the preparation method disclosed by the invention, monosaccharides such as fructose and glucose in the mulberry powder and the mogroside are removed by utilizing the growth of microorganisms such as lactic acid bacteria, so that the final yoghourt almost does not contain free monosaccharides, the alpha-glucosidase inhibitory activity of the final yoghourt is not influenced, and the yoghourt is suitable for being taken by people with hyperglycemia.

The application discloses an INS / GNSS rod arm error online estimation method based on dimension reduction filtering. The method splits the INS / GNSS rod arm error and other error variables, constructs multiple low-dimensional filters, reduces the coupling between the error variables, has clear mechanism, simplifies the model, reduces the requirements of the combined navigation algorithm on the chip main frequency and the storage space, improves the calculation speed and the filtering estimation precision, and can improve the positioning performance of the vehicle-mounted combined navigation system after the INS / GNSS rod arm error online estimation and compensation, and has high engineering application value.

The invention relates to the technical field of microbial infection treatment and molecular biology, and provides a method for inhibiting formation of a staphylococcus aureus biofilm, which is realized by inhibiting nitrosation modification of MgrA protein in staphylococcus aureus so as to inhibit formation of the biofilm. The inhibitor is nitric oxide synthase and is used for inhibiting nitrosation modification of the MgrA protein; the nitrosation modification for inhibiting the MgrA protein is realized by applying a nitric oxide synthase (NOS) inhibitor; the invention reveals that the staphylococcus aureus endogenous nitric oxide can promote the formation of a biofilm by modifying and regulating the 12th cysteine of the protein MgrA for the first time; based on the target spot, research proves that the MgrA modification level can be effectively reduced and the removal of the biofilm can be enhanced through multiple means such as gene mutation, NOS inhibitor or combination of the NOS inhibitor and antibiotics, and a new strategy and a potential drug development approach are provided for preventing and treating staphylococcus aureus intractable biofilm infection.

The invention discloses a crack-free heat treatment method for additive manufacturing of nickel-based superalloy, and relates to the technical field of heat treatment of superalloy, in particular to a crack-free heat treatment method for additive manufacturing of nickel-based superalloy. The invention aims to solve the technical problem that an existing additive manufacturing nickel-based superalloy is extremely easy to generate intergranular cracks in a subsequent heat treatment temperature rise stage, so that a component is directly scrapped. According to the method, the heating rate of the additive manufacturing nickel-based superalloy at the initial stage of heat treatment is increased to be not lower than 40 DEG C / min, so that precipitation of carbide M23C6 at the grain boundary in the heating process is effectively inhibited, heat treatment cracks are avoided, the method is particularly suitable for IN738LC alloy and other similar precipitation strengthening superalloy, and the method is suitable for industrial production. And an effective scheme is provided for realizing defect-free heat treatment of the additive manufacturing high-temperature alloy component. The rapid heating step can be used as pretreatment and is seamlessly connected with subsequent heat treatment for various purposes, and the application window is wide.

The invention belongs to the technical field of medicines, and particularly discloses application of targeted mesenchymal stem cell NAT10 and RNA acetylation in inflammatory diseases. The invention reveals for the first time that NAT10 mediated ac4C modification is a key positive regulatory factor for regulating the immune function of mesenchymal stem cells (MSC), and an inflammatory environment can inhibit the pathway so as to cause weakening of the MSC function. Based on the discovery, NAT10 expression in the MSC is up-regulated through a genetic means, and the MSC with the enhanced function is prepared. The cell shows a higher ac4C acetylation level, and the expression of key immunosuppressive factors iNOS and COX2 and the secretion of products NO and PGE2 are remarkably improved, so that a stronger T cell inhibition capability is obtained. Animal experiments show that the MSC with the enhanced function shows a remarkably superior treatment effect compared with common MSC in disease models of inflammatory liver injury, periodontitis and the like.

The application provides a composition and application of the composition in preparation of a product for maintaining blood lipid health level, the composition comprises 0.1-10 parts by weight of pentadecanoic acid, further comprises 1 part by weight of ginseng and / or 0.1-5 parts by weight of hawthorn, the composition is target-specific, components are mutually matched and synergistic, has the efficacy of maintaining blood lipid health level, realizes simultaneous effective intervention on two key indexes of total cholesterol and triglyceride; the composition has a clear mechanism, obvious synergistic effect, and outstanding advantages in safety, controllability of cost and feasibility of productization, and provides important material foundation and technical support for development of an efficient, balanced and easily popularized blood lipid health management scheme.

The application provides a composition and application thereof in preparation of a product for maintaining blood lipid health level, the composition comprising 1 part by weight of ginseng, 0.1-5 parts by weight of hawthorn and 0.1-10 parts by weight of two or three of phosphatidylcholine, the composition being target-specific, the components being mutually matched and synergistically effective, having the efficacy of maintaining blood lipid health level, realizing simultaneous effective intervention on two key indexes of total cholesterol and triglyceride; the composition has a clear mechanism, significant synergistic effect, and outstanding advantages in safety, controllability of cost and feasibility of productization, and provides important material basis and technical support for development of an efficient, balanced and easily promoted blood lipid health management scheme.

This invention discloses a combination of exosomal miRNA biomarkers for single embryo culture medium used to assess embryo implantation potential, its application, and a method for assessing embryo implantation potential, relating to the field of assisted reproductive technology. The biomarker combination includes the miRNA molecular markers hsa-miR-143-3p and hsa-miR-125a-5p, wherein the nucleotide sequence of hsa-miR-143-3p is 5′-UGAGAUGAAGCACUGUAGCUC-3′, and the nucleotide sequence of hsa-miR-125a-5p is 5′-UCCCUGAGACCCUUUAACCUGUGA-3′. This invention establishes a non-invasive method for detecting embryo implantation potential, revealing significantly elevated expression of hsa-miR-143-3p and hsa-miR-125a-5p in non-pregnant EVs. Experimental data show that the expression levels of hsa-miR-143-3p and hsa-miR-125a-5p are significantly correlated with embryo implantation potential, serving as key indicators for assessing embryo implantation potential. This method can effectively distinguish between embryos with successful and failed pregnancies, providing new biomarkers for embryo implantation potential assessment. Compared with existing technologies, this method has advantages such as being non-invasive, highly specific, and having a clear mechanism, providing important technical support for improving the success rate of assisted reproductive technology and developing personalized treatment strategies.

The application relates to a MYH2-FIGN interaction blocking amino acid compound and an application thereof, and relates to the fields of polypeptide drugs and tumor prevention and treatment. The MYH2-FIGN interaction blocking amino acid compound is an amino acid sequence as shown in SEQ ID NO:1 or SEQ ID NO:3 or a polypeptide derivative thereof. The application further discloses a pharmaceutical composition containing the MYH2-FIGN interaction blocking amino acid compound and a pharmaceutically acceptable carrier. The application first designs a polypeptide drug targeting the MYH2-FIGN interaction interface, and compared with traditional small molecules, the polypeptide drug has specificity for MYH2-FIGN interaction inhibition, and through fusion of an iRGD peptide, not only is the polypeptide cell penetration problem solved, but also the polypeptide drug is endowed with tumor targeting, the bioavailability of the drug is improved, and the systemic toxicity is reduced. The polypeptide drug can adopt various drug dosage forms, and the preparation process is simple.

The invention discloses an application of Elov16 as a drug target in preparation or screening of drugs for treating anxiety and / or depression related diseases. The invention finds that Elov16 affects anxiety behavior by regulating and controlling fatty acid composition of a prefrontal cortex. According to the present invention, the Elovl6 function deficiency results in the increase of palmitic acid and the reduction of oleic acid and linoleic acid, such that the PPARalpha-Allopregnanolone signal axis is inhibited, the neurosteroid Allopregnanolone synthesis is reduced, and the anxiety-like behavior is finally induced; the PPARalpha agonist GW7647 can be used for recovering the level of Allopregnanolone and remarkably improving the anxiety phenotype of an Elov16 knockout mouse. The invention provides a drug screening and diagnosis marker system taking Elov16 as a target spot and a precise intervention strategy for treating fatty acid metabolism disorder related anxiety diseases by activating a PPARalpha-Allopregnanolone pathway, and provides a theoretical basis and a conversion medical pathway for the development of anti-anxiety drugs.

The invention discloses a cigarette smoke sweetness improving method based on bitterness masking and sweetness synergistic regulation, which comprises the following steps: preparing a dual regulating agent: compounding a bitterness masking agent and a sweetness synergist according to a preset mass ratio, and dissolving or dispersing in a solvent to form the dual regulating agent; the dual regulating agent is applied to a cigarette carrier, and the cigarette material is obtained; and balancing the treated cigarette material and rolling the cigarette material into cigarettes. According to the cigarette smoke sweetness improving method based on bitter taste masking and sweetness synergistic regulation, starting from the system level of sensory interaction, through a dual sensory regulation mechanism of bitter taste masking and sweetness synergistic, the cigarette smoke sweetness perception is synergistically improved, the sweetness signal is improved, meanwhile, background bitter taste interference is effectively inhibited, and the cigarette smoke sweetness improving effect is achieved. Therefore, efficient, pure and natural improvement of the sweetness is realized; the defects of low efficiency and unnatural sweetness of the traditional single sweetening agent are overcome, and a novel way which is more systematic, more efficient and capable of comprehensively improving the taste of the smoke is provided.

The invention discloses application of a CCNB1 phosphorylation inhibition tool in preparation of a medicine for preventing and / or treating triple negative breast cancer, and particularly relates to application of a CCNB1 protein S128 site phosphorylation inhibitor in preparation of a medicine for preventing and / or treating triple negative breast cancer. The phosphorylation inhibitor refers to a tool or means used in the process of blocking or weakening protein phosphorylation, particularly a gene editing system, and the 128 serine Ser coded by a CCNB1 gene is mutated into alanine Ala which is recorded as S128A in a target cell. Compared with a traditional small molecule drug, the gene editing tool accurately intervenes phosphorylation modification at the gene level, has the advantages of being clear in mechanism, high in targeting performance, high in safety and the like, is suitable for basic research and potential clinical transformation of TNBC targeted therapy, and has a good application prospect.

The invention provides an application of 3-hydroxyl capryloyl carnitine as a target spot of metabolic liver diseases after cholecystectomy. Through Mendel randomization and mediation analysis of two samples, it is confirmed for the first time that 3-hydroxycapryloyl carnitine is a key causal mediating factor for risk increase of MASLD caused by cholecystectomy, and the mediating proportion is 10.37%. It is directly proved that the disease progress can be affected by intervening the metabolite level.

This invention belongs to the field of microbial technology and discloses a strain of Bacillus subtilis HJKC1901 and its application in the prevention and treatment of cryptosporidiosis in calves. The strain's preservation number is CGMCC NO.33395. It was isolated using a targeted screening method with intestinal epithelial cell barrier function repair as the core indicator. It can simultaneously and stably secrete six characteristic proteins (glucuronide xylanase, 6-phosphate maltosidase, extracellular small protease, subtilis metalloproteinase, catalase, and gamma-glutamyl transferase precursor enzyme). When this strain is formulated into a microbial preparation and added to calf feed or drinking water, it can significantly reduce the diarrhea rate and oocyst excretion in calves infected with Cryptosporidium, repair intestinal barrier damage, and provide a novel biotechnological solution for the prevention and treatment of cryptosporidiosis in calves.

The invention discloses a marker and a system for predicting the effect of tumor immunotherapy, and relates to the field of tumor immunotherapy, the marker is expressed in various immune cells, participates in activation of the immune cells, is an important effector protein of a human innate immune system, namely LCP2, and can be detected in a tissue sample from a tumor patient. The clinical effect of tumor immunotherapy is predicted by detecting the infiltration level of killer immune cells in tumors.