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Microfluidic chip for study of tumor single cell invasion and epithelial-mesenchymal transition

A microfluidic chip, single-cell technology, applied in laboratory containers, specific-purpose bioreactors/fermenters, biochemical instruments, etc., can solve the problem of lack of high-throughput detection methods, and improve the success of experiments. efficiency and reliability, reducing operating steps, simplifying the effect of operating steps

Active Publication Date: 2017-12-22
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among the currently disclosed technologies, there is no high-throughput detection method for the invasion ability and EMT process analysis of single tumor cells

Method used

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  • Microfluidic chip for study of tumor single cell invasion and epithelial-mesenchymal transition
  • Microfluidic chip for study of tumor single cell invasion and epithelial-mesenchymal transition

Examples

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Embodiment 1

[0036] Example 1 Microfluidic chip for studying tumor single cell invasion and epithelial-mesenchymal transition and its preparation method

[0037] The microfluidic chip provided in this embodiment includes a four-layer structure stacked together and sealed with each other, from top to bottom are the top cover, the microfluidic channel layer, the substrate with a sorting array, and the cavity with a cavity. The base plate, the sorting unit on the base plate and the cavity on the base plate are arranged oppositely; the sorting unit has a square micro-well on the base plate for obtaining a single cell; the square micro-well vertically penetrates the base plate . The bottom of the square microwell is a square bottom plate provided with several smaller microholes, and the square bottom plate is connected with the substrate as a whole. The specific structure is:

[0038] 1. Top cover and fluid channel

[0039] When identifying cells, light needs to pass through the top cover, so...

Embodiment 2

[0074] The microfluidic chip provided in this embodiment includes a four-layer structure stacked together and sealed with each other, from top to bottom are the top cover, the microfluidic channel layer, the substrate with a sorting array, and the cavity with a cavity. The base plate, the sorting unit on the base plate and the cavity on the base plate are arranged oppositely; the sorting unit has a square micro-well on the base plate for obtaining a single cell; the square micro-well vertically penetrates the base plate . The bottom of the square microwell is a square bottom plate provided with several smaller microholes, and the square bottom plate is connected with the substrate as a whole.

[0075] 1. Top cover and fluid channel

[0076] The top cover is provided with a sample inlet and a sample outlet; the microfluidic channel layer is provided with a flow channel, and the flow channel has a long strip shape with a wide middle section and narrower ends at both ends, and th...

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Abstract

The invention provides a microfluidic chip for studying the tumor single cell invasion and epithelial-mesenchymal transition process capability. The microfluidic chip includes four layers of structures that are stacked together in order and sealed to each other, and falls into a top cover, a microfluidic channel layer, a substrate with micropore array and a baseplate with a cavity group from top to bottom. The invention also provides a system that matches with the chip and is used for single cell detection. The microfluidic chip and the corresponding detection system provided by the invention can achieve rapid, simple and low-cost sorting of single cells and study of invasion ability and epithelial-mesenchymal transition, and performs gene analysis on a target single cell.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, in particular to a microfluidic chip for studying tumor cell invasion ability and epithelial-mesenchymal transition Background technique [0002] In recent years, the morbidity and mortality of cancer have risen rapidly, and it has become an internationally recognized disease with the number one fatality rate. In the death cases of cancer, the spread and metastasis of cancer are the main factors leading to death. Therefore, the study of the mechanism of tumor spread and metastasis is of great help to understand the occurrence and development of cancer and to overcome cancer. [0003] More than 90% of tumor cells originate from epithelial cells. Epithelial cells need to be transformed into mesenchymal cells with migration and invasion ability in the process of diffusion and metastasis. The physiological phenomenon that polarized epithelial cells lose their polarity and transform into...

Claims

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Application Information

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IPC IPC(8): B01L3/00C12M1/34C12M1/00G01N21/64
CPCB01L3/5027B01L2200/10B01L2300/0887C12Q1/6886C12Q2600/158G01N21/6428
Inventor 李仁马艳胡志远魏泽文
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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