Microfluidic chip for study of tumor single cell invasion and epithelial-mesenchymal transition
A microfluidic chip, single-cell technology, applied in laboratory containers, specific-purpose bioreactors/fermenters, biochemical instruments, etc., can solve the problem of lack of high-throughput detection methods, and improve the success of experiments. efficiency and reliability, reducing operating steps, simplifying the effect of operating steps
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[0036] Example 1 Microfluidic chip for studying tumor single cell invasion and epithelial-mesenchymal transition and its preparation method
[0037] The microfluidic chip provided in this embodiment includes a four-layer structure that is stacked together and sealed with each other. From top to bottom, there are a top cover, a microfluidic channel layer, a substrate with a sorting array, and a cavity with a cavity. The bottom plate, the sorting unit on the base plate and the cavity on the bottom plate are oppositely arranged; the sorting unit is provided with a square micro-well on the top of the base plate, which is used to obtain a single cell; the square micro-well vertically penetrates the base plate . The bottom of the square micro-well is a square bottom plate with several smaller micro-holes, and the square bottom plate is connected with the substrate as a whole. The specific structure is:
[0038] 1. Top cover and fluid channel
[0039] When identifying cells, light ...
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[0073] Example 2
[0074] The microfluidic chip provided in this embodiment includes a four-layer structure that is stacked together and sealed with each other. From top to bottom, there are a top cover, a microfluidic channel layer, a substrate with a sorting array, and a cavity with a cavity. The bottom plate, the sorting unit on the base plate and the cavity on the bottom plate are oppositely arranged; the sorting unit is provided with a square micro-well on the top of the base plate, which is used to obtain a single cell; the square micro-well vertically penetrates the base plate . The bottom of the square micro-well is a square bottom plate with several smaller micro-holes, and the square bottom plate is connected with the substrate as a whole.
[0075] 1. Top cover and fluid channel
[0076] The top cover is provided with a sample inlet and a sample outlet; a flow channel is opened on the microfluidic channel layer, and the flow channel has a long shape with a wide midd...
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