153results about How to "Improve the success rate of experiments" patented technology

The invention provides a micro fluidic chip for sorting and whole genome amplification of a single cell. The micro fluidic chip comprises a four-layer structure, wherein the four layers are stacked together in order and mutually sealed, and the structure comprises the following layers from top to bottom: a top cover, a micro valve control layer, a valve plate layer and a channel layer; and the bottom of the micro fluidic chip is externally provided with a magnet. The invention also provides a system for screening and identifying cells as well as amplification and analysis of a single cell, and the system is matched with the chip. The micro fluidic chip and the corresponding detection system can be used for sorting target cells from a large amount of cells quickly, simply and cheaply, and amplifying and analyzing whole genome DNA of a single cell therein.

The present invention provides a microfluidic chip for sorting single cells expressing EGFR and detecting multiple gene loci. The microfluidic chip includes a four-layer structure stacked together and sealed with each other, from top to bottom They are respectively a top cover, a microfluidic channel layer, a substrate with a microhole array and a bottom plate with a cavity group. The invention also provides a system for single-cell level detection of multiple mutation sites of EGFR gene matched with the chip. The microfluidic chip and the corresponding detection system of the present invention can quickly, easily and cheaply sort target cells from a large number of cells, and perform EGFR multi-gene site analysis on a single cell among them.

The invention discloses an intelligent magnetic frame with an adjustable position. The intelligent magnetic frame comprises a transverse moving mechanism and a longitudinal moving mechanism, wherein the transverse moving mechanism comprises a movable platform bracket, and a driving belt wheel and a driven belt wheel which are arranged at the two ends of the movable platform bracket; the driving belt wheel is connected with a driving wheel motor; the driving belt wheel and the driven belt wheel are engaged with a synchronous tooth-shaped belt; the movable platform bracket is further provided with a linear guide rail; the linear guide rail is provided with a magnet fixing frame; the magnet fixing frame is provided with a plurality of magnets; the magnet fixing frame is connected with the synchronous tooth-shaped belt; the longitudinal moving mechanism comprises a fixed shaft hoisting motor; the fixed shaft hoisting motor is fixed on a motor mounting plate; the fixed shaft hoisting motor enables the movable platform bracket to move up and down. The magnetic frame can move up and down so that the adaptability of the magnetic frame to magnetic beads from different factories is greatly improved and a phenomenon that the magnetic beads are clustered caused by the over-large magnetism of the magnets is avoided. The magnetic frame can move left and right so that sampling and adsorption of automatic equipment can be carried out at the same time, the speed for treating samples by the automatic equipment is increased, a lot of time is saved and the success rate of experiments is improved.

The invention discloses a method for reducing perforation speed of nucleic acid molecules in a nanopore sequencing method. The method comprises the following steps of: adding the nucleic acid molecules to be measured into a nanopore sequencing device filled with electrolyte, wherein the nanopore sequencing device comprises an electrolytic cell provided with an anode and a cathode, and a solid nanopore film for separating the anode from the cathode of the electrolytic cell; placing the nucleic acid molecules to be measured in a cathode cavity of the electrolytic cell,; and when the nucleic acid molecules are measured, applying the voltage between the anode and the cathode, and introducing a reverse external field taking a pressure intensity external field as an electric field simultaneously. According to the method, the pressure intensity external field is reversely applied under the drive of an additional electric field, so that the speed of DNA (Deoxyribonucleic Acid) molecules is reduced when the DNA molecules pass through the solid nanopore, and the speed is reduced by about 50% to 80%, thus the time resolution of a DNA unimolecule detection technology is greatly improved. The method has very bright application prospect on a solid nanopore DNA molecule sequencing device.

The invention provides a discount coupon push method, a discount coupon push system, a storage medium, an electronic device and a stream splitting method. The discount coupon push method includes the following steps that: user streams are randomly split into a plurality of groups, discount coupons of different denominations are pushed to the groups, and the order placing data of the groups are put into statistics; experimental labels are set according to historical order data, wherein each experimental label contains a plurality of mutually-exclusive label items; one or more label items are configured for each user stream in a matched manner; user streams are extracted according to the label items of a single experimental label or the label item combinations of a plurality of experimental labels so as to form different experiment groups; user streams in each experiment group are split into a plurality of sub experiment groups according to the discount coupons of different denominations, and the order placing ratios of the user streams of the sub experiment groups are put into statistics; and discount coupons of denominations corresponding to a sub experiment group with the highest order placing ratio are pushed to the user streams in the experiment groups. With the discount coupon push method, the discount coupon push system, the storage medium, the electronic device and the stream splitting method of the invention adopted, stream splitting experiment efficiency can be improved, an experiment period can be shortened, and experiment costs can be reduced greatly.

This invention provides one handle virtual experiment chamber system, which comprises signal control process unit and signal control process unit connected input unit, memory unit and display unit to receive user input and operation orders, wherein, the signal control process unit further comprises central processor unit and one virtual experiment platform module for ratification operation. This invention provides one handle virtual experiment chamber realizing method.

The invention discloses an intelligent laboratory management system. The system comprises a database used for storing identification comparison information of people participating in an experiment andteaching comparison information, a human body characteristic identification module used for collecting human body characteristic information of people getting in and out of a laboratory, a safety monitoring module used for performing comparison between the human body characteristic information with information in the database to control accessing permission according to a comparison result and count the number of accessing people, an image processing module used for conducting gray processing on images of each experiment desktop to obtain the gray images of each experiment desktop, an image comparison module used for comparing the gray images of each experiment desktop with preset comparison images to judge whether or not the number of experimental items is a preset number, and a reminding module used for sending reminding notices to people performing the experiment and a teacher when the judging result of the image comparison module is negative.

The invention relates to an isolated organ perfusion system, which comprises a liquid storage container, a centrifugal pump, a membrane lung, a first peristaltic pump, a leukocyte filter, a hemodialyzer and a waste liquid bag, wherein the liquid inlet end of the first peristaltic pump is communicated with the liquid storage container through a pipeline, the liquid outlet end of the first peristaltic pump is communicated with the liquid inlet end of the hemodialyzer through a pipeline, the liquid outlet end of the hemodialyzer is communicated with the liquid storage container through a pipeline, the waste discharge end of the hemodialyzer is communicated with the waste liquid bag through a pipeline, the liquid storage container, the first peristaltic pump and the hemodialyzer form a filtering circulation auxiliary path, and the leukocyte filter is arranged on the filtering circulation auxiliary path. According to the invention, when the leukocyte filter is blocked due to long-time work,the leukocyte filter can block the filtering circulation auxiliary path and cannot block the perfusion circulation main path, the perfusion circulation main path can still continue to work at the moment, the in-vitro organs can be continuously protected, and the leukocyte filter on the filtering circulation auxiliary path can be replaced in time.

The invention provides a microfluidic chip for studying the tumor single cell invasion and epithelial-mesenchymal transition process capability. The microfluidic chip includes four layers of structures that are stacked together in order and sealed to each other, and falls into a top cover, a microfluidic channel layer, a substrate with micropore array and a baseplate with a cavity group from top to bottom. The invention also provides a system that matches with the chip and is used for single cell detection. The microfluidic chip and the corresponding detection system provided by the invention can achieve rapid, simple and low-cost sorting of single cells and study of invasion ability and epithelial-mesenchymal transition, and performs gene analysis on a target single cell.

The invention discloses a simulation experiment device for coal mine gas dynamic disaster under a simulation environment. The device comprises a loading frame, wherein a pneumatic hammer and two Z direction loading hydraulic cylinders are mounted on the loading frame, and a Z direction loading plate is connected between the bottom ends of piston rods of the two Z direction loading hydraulic cylinders; a Y direction guide rail and an X direction guide rail are connected to the bottom of the loading frame; a protruding cylinder body and a Y direction loading hydraulic cylinder are arranged on the Y direction guide rail, and an X direction loading hydraulic cylinder is arranged on an X direction motor trolley; a Z direction loading port is formed in the top of the protruding cylinder body, a Y direction cylinder and an X direction cylinder are connected to the side wall of the protruding cylinder body, and a protruding port is formed in the upper surface of the X direction cylinder; a fourth pipeline with an opened tail end is connected to the X direction cylinder; a vacuum-pumping system and a gas loading system are connected to the protruding cylinder body. The invention further discloses an experiment method using the device, coal mine rock burst, gas flow field, overlaying strata stress and horizontal tectonic stress can be simulated, the experiment simulation degree is improved, and the experiment success rate and the accurate degree are improved.

The invention discloses a detection method for biomolecular probe-calibrated specific sites of DNA based on a nanopore device and application thereof. The method comprises the following steps: 1) calibrating the specific sites of DNA by using a biomolecular probe so as to obtain calibrated DNA; and 2) detecting the specific sites of the calibrated DNA by using a nanopore sequencing device, wherein the calibrated DNA is added into a positive electrode chamber filled with an electrolyte, an external pressure field is introduced to the positive electrode chamber during detection and is used as an external driving field for DNA perforation, voltage is applied between a positive electrode and a negative electrode and used as an external electric field with a direction opposite to the direction of the external pressure field, and an active force of the external pressure field on the DNA in a nanopore during detection is greater than an electric field force. With the method provided by the invention, secondary current signals totally different from those of common unmodified DNA are obtained, the spatial positions and sequence of modified specific sites of the DNA can be characterized through analysis and statistics of the secondary current signals, so a physical map of the long chain DNA is obtained.

The invention discloses a micro-sample preparation device for a frozen electron microscope and a sample preparation method. The micro-sample preparation device comprises a supporting net assembly witha sample preparation operation state and a freezing operation state, a sample preparation probe used for respectively carrying out sampling operation and completing sample preparation operation on ato-be-detected sample solution on the supporting net assembly, a three-dimensional translation platform used for driving the sample preparation probe to be converted between the sampling operation andthe sample preparation operation, a freezing module used for carrying out freezing operation on the sample solution on the supporting net assembly, and a rapid transfer device used for driving the supporting net assembly to transfer between the sample preparation operation state and the freezing operation state. According to the invention, a frozen electron microscope sample preparation experiment with ultramicro sample consumption can be realized, and the consumption of a single sample liquid drop is in a nano-liter level and below the nano-liter level. Compared with a conventional sample preparation robot, the sample consumption is reduced by 1000 times or above, the experiment cost is remarkably reduced, and the protein sample purification period is shortened. The success rate of frozen electron microscope sample preparation and the reproducibility of experiments are obviously improved.

The invention relates to a split type miniature peristaltic pump, in particular to a miniature peristaltic pump, wherein a pipeline is integrated to a pump head, and both the pump head and the pipeline can be used for one time. The micropump comprises a pump body, a motor and a pump head and pipeline assembly. The pump head and pipeline assembly comprises a pump head seat, an eccentric wheel, a pump head roller and a circumferential pressing pipeline; a pump head seat center hole is formed in the pump head seat, a through hole is coaxially formed in the bottom of the pump head seat center hole, and a pipeline inlet/outlet groove is formed in one side of the pump head seat; the pump head roller is arranged in the pump head seat center hole, a pump head roller center hole is formed in the pump head roller, and the eccentric wheel is arranged in the pump head roller center hole and connected with an output shaft of the motor through an eccentric hole; and a pump head roller groove is formed in the outer wall of the pump head roller, and the circumferential pressing pipeline is sleeved with the pump head roller groove along the outer wall of the pump head roller. According to the micropump, the structure of the assembly formed by the pump head and the pipeline can guarantee that the pump is compact in structure and small in size; and the single-use purpose can be achieved, the pollution risk is lowered effectively, and the experiment success rate is increased to a great extent.

The invention discloses an experimental method for isothermal local loading formation of a titanium alloy. By adopting a terrace die to increase the stability of a die and a blank which are matched, the problem that a test sample and an arc-shaped die are easy to deviate to be unstabilized in the assembling process is solved, and the experimental success rate is remarkably increased. Inhomogeneous deformation of a local loading formation transition region is realized by way of loading in steps by using the terrace die and not using the terrace die. Die processing and experimental operation are simple, convenient and feasible and well reflect the deformation characteristic of the transitional region in local loading formation. According to the experimental method disclosed by the invention, the deformation gradient of the blank is more uniform, easy to control and convenient for subsequent tissue observation of a special deformation region compared with that of an arc-shaped die, and thus a good experimental foundation is provided for quantitative research which discloses microstructure development mechanism and rule in the transitional region in isothermal local loading formation of the titanium alloy.

The invention relates to a micro-seismic response coal and gas outburst self-trigger simulation experiment device. The device comprises a closed outburst cavity composed of a rear end plate, a cylindrical outer plate and a pressure loading movable piston together, a gas pressure sensor and a stress sensor are arranged in a hole of the cylindrical outer plate, an outburst opening is formed in the rear end plate, special sealing materials are arranged in the outburst opening and in the cylindrical outer plate for sealing, the materials are good in rheological property and can automatically trigger coal and gas outburst, and a micro-seismic monitoring device is arranged outside the cavity to capture the foreboding information of coal and gas outburst. According to the device, loads or gas pressure is increased on coal samples in the outburst cavity continuously so as to induce the coal samples in the outburst cavity to automatically trigger the outburst from the outburst opening, features of the coal and gas outburst excitation stage are recorded in detail, changes of coal sample stress fields and gas fields in the coal and gas outburst excitation stage are visually reflected, and solid theoretical bases are provided for reasonable and scientific prediction in selection and production processes.

The invention discloses a rigid and flexible two-stage locking ball joint. A two-stage piston is arranged in the ball joint; a two-stage locking state is realized; when the fluid pressure is not sufficient to simultaneously push the two-stage piston, the ball joint is in a flexible locking state; when the fluid pressure can simultaneously push the two-stage piston, the ball joint is in a rigid locking state. Through a connecting sleeve, the ball joint is fixed with the head end and the tail end of the sleeve to form a ball joint connecting rod; then, the two or a plurality of ball joint connecting rods are connected; a multi-freedom operation arm can be formed; a tool or an operation appliance can be positioned; when different pressure is exerted on each connecting rod, the locking state of the ball joint can be controlled so as to adapt to different joint loads.

The invention discloses a testing device and method for rock straight shear-seepage, and relates to a rock mechanics test technique. An involved three-axis compression shear testing device comprises an axial shear pressing head (1), a pressure chamber (3), sealing tape (4), a deformation rubber sleeve (5) for isolating contact between a test piece and oil, the testing test piece (6) with a prefabricated joint, and a rigid cylindrical base plate (8). According to the adopted testing method and test piece preparation method, the characteristics of a rock three-axis compression experiment are fully utilized, the stable confining pressure is provided for the test piece through the oil pressure chamber, the rock test piece with the prefabricated joint is prepared through the special method, meanwhile, the conditions for a shear test are created, and then a seepage test is carried out. By adopting the method to carry out the shear-seepage tests through a three-axis testing instrument, obtained data are more accurate and more reliable, and the shear damage condition of rock under the natural conditions is simulated to a larger extent.

The invention discloses a rat brain stereotaxic fitting device. A work table is provided with a first step surface and a second step surface from top to bottom; two upright posts are arranged at the position, along the width, on the first step surface; limit holders are fixedly connected to the positions, at the same height, of the same sides of the two upright posts; each limit holder is providedwith a through hole of which the axis direction parallel to the width of the first step surface; an ear bar is inserted in each through hole in a sliding adjustable manner; the opposite ends of the two ear bars are tip ends. An adjusting mechanism can realize sliding adjustment along the length of the second step surface and is connected with a front tooth rod; the front tooth rod comprises a horizontal plate and a vertical bar; the vertical bar is arranged on the adjusting mechanism and capable of sliding for adjustment along the vertical direction, one end of horizontal plate is fixedly connected to the top end of the vertical bar, the other end of the horizontal plate is provided with a front tooth hole and extends along a gap between the two upright posts. The fitting device is used for fixing rat's head for biological study tests.

The invention discloses a method and a device for charging air for a diamond butting pressure anvil. The air charging method comprises: making gas highly dense and even liquefied by a low-temperature cooling means; then making the gas enter sealing cavities among diamond pads in a cooled DAC device, and ensuring the gas fully fills the cavity at the pressure slightly higher than the atmospheric pressure; tightly locking the DAC device through external force; and sealing the gas to be charged into a sample cavity. The device for charging air for the diamond butting pressure anvil comprises a diamond butting pressure anvil body, a gas introducing device, a sealing device and a cooling device. The method can make air flow in the sample cavity more stable, can obviously improve experiment success rate and can greatly reduce unnecessary gas loss at the same time.

The invention discloses an automatic transmitting sample grinding robot which comprises a base, a support, a rotary platform, a rotary motor and the like. The support is installed on one side of the base. The rotary motor is installed in the center of the base. A main shaft of the rotary motor is arranged vertically upward. The rotary platform is coaxially mounted on the main shaft of the rotary motor. An abrasive paper clamping ring is mounted on the circumference of the rotary platform in a clamped mode. A cylinder body of an electric lifting cylinder is vertically mounted at the top of the support. A mounting table top rod is coaxially installed in the center of a sample mounting table. A water spray tube and a grinding fluid tube are mounted on the two sides of the lower portion of the support correspondingly. An outlet of the water spray tube and an outlet of the grinding fluid tube are located on the two sides of the rotary platform correspondingly. According to the automatic transmitting sample grinding robot, the disciform sample mounting table and the automatic electric lifting cylinder are arranged to achieve the purpose of automatic grinding of transmitting samples and improve the experiment efficiency and the success rate; and the automatic transmitting sample grinding robot is high in efficiency and convenient to operate.

A Pickering emulsion is prepared by using a cellulose derivative which is one of ethyl cellulose, cellulose acetate, cellulose acetate propionate, cellulose acetate butyrate and acetylated nanocrystalline cellulose a solid emulsifier and using pollution-free edible oil as an oil phase, and the cellulose derivative has hydrophobic groups, so that the stability of the oil-in-water Pickering emulsionis facilitated. The acetylated modified nanocrystalline cellulose has strong interaction with an oil phase, so the stabilizing effect is good, the droplet size of the Pickering emulsion is significantly reduced, and the particle size distribution of the emulsion is uniform.