153results about How to "Improve the success rate of experiments" patented technology

The invention provides a micro fluidic chip for sorting and whole genome amplification of a single cell. The micro fluidic chip comprises a four-layer structure, wherein the four layers are stacked together in order and mutually sealed, and the structure comprises the following layers from top to bottom: a top cover, a micro valve control layer, a valve plate layer and a channel layer; and the bottom of the micro fluidic chip is externally provided with a magnet. The invention also provides a system for screening and identifying cells as well as amplification and analysis of a single cell, and the system is matched with the chip. The micro fluidic chip and the corresponding detection system can be used for sorting target cells from a large amount of cells quickly, simply and cheaply, and amplifying and analyzing whole genome DNA of a single cell therein.

The invention provides a single-cell-sorting and multigene-locus-detection micro-fluidic chip for expressing EGFR. The micro-fluidic chip comprises a top cover, a micro-fluid channel layer, a substrate and a base plate which are sequentially stacked from top to bottom and sealed with one another, wherein the substrate is provided with micro-pore arrays and the base plate is provided with cavity groups. The invention further provides a system matched with the chip and used for detecting the single cell level of multiple mutation sites of EGFR genes. The micro-fluidic chip and the corresponding detecting system can screen out target cells from a large amount of cells fast, simply and cheaply and perform EGFR multi-gene-site analysis on the single cell in the target cells.

The invention discloses a method for carrying out deceleration and monomolecular capture on a nucleic acid molecule based on a solid-state nano hole. The method comprises the following steps of: adding the nucleic acid molecule to be measured into a nano hole sequencing device filled with an electrolyte, wherein the nano hole sequencing device comprises an electrolytic bath provided with an anode and a cathode, and a solid-state nano hole thin film for separating the anode of the electrolytic bath from the cathode of the electrolytic bath; placing the nucleic acid molecule in a positive chamber of the electrolytic bath; during measurement, introducing a pressure external field into the positive chamber as a driving external field for the perforation of the nucleic acid molecule; applying a voltage between the anode and the cathode as an electric field external field reverse to the pressure external field; and in a measurement process, enabling the acting force of the pressure external field, from which the nucleic acid molecule is suffered in the nano hole to be more than or approximately equal to electric field force according to a deceleration purpose or a capture purpose to be realized by the method. By using the method, on the premise that the signal-to-noise ratio of a measurement signal is not decreased, the speed at which a DNA (deoxyribonucleic acid) molecule penetrates through a nano hole device can be effectively decreased, and a perforation speed can be slowed down for above one order of magnitude.

The invention discloses an intelligent magnetic frame with an adjustable position. The intelligent magnetic frame comprises a transverse moving mechanism and a longitudinal moving mechanism, wherein the transverse moving mechanism comprises a movable platform bracket, and a driving belt wheel and a driven belt wheel which are arranged at the two ends of the movable platform bracket; the driving belt wheel is connected with a driving wheel motor; the driving belt wheel and the driven belt wheel are engaged with a synchronous tooth-shaped belt; the movable platform bracket is further provided with a linear guide rail; the linear guide rail is provided with a magnet fixing frame; the magnet fixing frame is provided with a plurality of magnets; the magnet fixing frame is connected with the synchronous tooth-shaped belt; the longitudinal moving mechanism comprises a fixed shaft hoisting motor; the fixed shaft hoisting motor is fixed on a motor mounting plate; the fixed shaft hoisting motor enables the movable platform bracket to move up and down. The magnetic frame can move up and down so that the adaptability of the magnetic frame to magnetic beads from different factories is greatly improved and a phenomenon that the magnetic beads are clustered caused by the over-large magnetism of the magnets is avoided. The magnetic frame can move left and right so that sampling and adsorption of automatic equipment can be carried out at the same time, the speed for treating samples by the automatic equipment is increased, a lot of time is saved and the success rate of experiments is improved.

The invention discloses a method for reducing perforation speed of nucleic acid molecules in a nanopore sequencing method. The method comprises the following steps of: adding the nucleic acid molecules to be measured into a nanopore sequencing device filled with electrolyte, wherein the nanopore sequencing device comprises an electrolytic cell provided with an anode and a cathode, and a solid nanopore film for separating the anode from the cathode of the electrolytic cell; placing the nucleic acid molecules to be measured in a cathode cavity of the electrolytic cell,; and when the nucleic acid molecules are measured, applying the voltage between the anode and the cathode, and introducing a reverse external field taking a pressure intensity external field as an electric field simultaneously. According to the method, the pressure intensity external field is reversely applied under the drive of an additional electric field, so that the speed of DNA (Deoxyribonucleic Acid) molecules is reduced when the DNA molecules pass through the solid nanopore, and the speed is reduced by about 50% to 80%, thus the time resolution of a DNA unimolecule detection technology is greatly improved. The method has very bright application prospect on a solid nanopore DNA molecule sequencing device.

The invention provides a discount coupon push method, a discount coupon push system, a storage medium, an electronic device and a stream splitting method. The discount coupon push method includes the following steps that: user streams are randomly split into a plurality of groups, discount coupons of different denominations are pushed to the groups, and the order placing data of the groups are put into statistics; experimental labels are set according to historical order data, wherein each experimental label contains a plurality of mutually-exclusive label items; one or more label items are configured for each user stream in a matched manner; user streams are extracted according to the label items of a single experimental label or the label item combinations of a plurality of experimental labels so as to form different experiment groups; user streams in each experiment group are split into a plurality of sub experiment groups according to the discount coupons of different denominations, and the order placing ratios of the user streams of the sub experiment groups are put into statistics; and discount coupons of denominations corresponding to a sub experiment group with the highest order placing ratio are pushed to the user streams in the experiment groups. With the discount coupon push method, the discount coupon push system, the storage medium, the electronic device and the stream splitting method of the invention adopted, stream splitting experiment efficiency can be improved, an experiment period can be shortened, and experiment costs can be reduced greatly.

The invention provides a multifunctional micro-fluidic chip used for simultaneous screening of a plurality of drugs and cells, belonging to the technical field of micro-fluidic chips. The multifunctional micro-fluidic chip used for simultaneous screening of a plurality of drugs and cells is composed of an upper PDMS micro-valve-controlled channel layer, a middle PDMS fluid channel layer and a lower glass substrate layer exerting supporting and cell attaching effect, wherein the PDMS micro-valve-controlled channel layer comprises a first micro-valve group and a second micro-valve group; the PDMS fluid channel layer comprises an array cell culture zone; and the PDMS fluid channel layer undergoes thermal bonding and is then integrated with the glass substrate layer through plasma bonding. The array cell culture zone is composed of a plurality of culture units and multiple stages of branch fluid sample introduction channels; the first micro-valve group is composed of linked micro-valves; and the second micro-valve group is composed of a plurality of independent micro-valves used for controlling connection and disconnection of a sample introduction port in a cell cavity with an S-shaped bent channel.

This invention provides one handle virtual experiment chamber system, which comprises signal control process unit and signal control process unit connected input unit, memory unit and display unit to receive user input and operation orders, wherein, the signal control process unit further comprises central processor unit and one virtual experiment platform module for ratification operation. This invention provides one handle virtual experiment chamber realizing method.

The invention discloses an intelligent laboratory management system. The system comprises a database used for storing identification comparison information of people participating in an experiment andteaching comparison information, a human body characteristic identification module used for collecting human body characteristic information of people getting in and out of a laboratory, a safety monitoring module used for performing comparison between the human body characteristic information with information in the database to control accessing permission according to a comparison result and count the number of accessing people, an image processing module used for conducting gray processing on images of each experiment desktop to obtain the gray images of each experiment desktop, an image comparison module used for comparing the gray images of each experiment desktop with preset comparison images to judge whether or not the number of experimental items is a preset number, and a reminding module used for sending reminding notices to people performing the experiment and a teacher when the judging result of the image comparison module is negative.

The invention relates to an isolated organ perfusion system, which comprises a liquid storage container, a centrifugal pump, a membrane lung, a first peristaltic pump, a leukocyte filter, a hemodialyzer and a waste liquid bag, wherein the liquid inlet end of the first peristaltic pump is communicated with the liquid storage container through a pipeline, the liquid outlet end of the first peristaltic pump is communicated with the liquid inlet end of the hemodialyzer through a pipeline, the liquid outlet end of the hemodialyzer is communicated with the liquid storage container through a pipeline, the waste discharge end of the hemodialyzer is communicated with the waste liquid bag through a pipeline, the liquid storage container, the first peristaltic pump and the hemodialyzer form a filtering circulation auxiliary path, and the leukocyte filter is arranged on the filtering circulation auxiliary path. According to the invention, when the leukocyte filter is blocked due to long-time work,the leukocyte filter can block the filtering circulation auxiliary path and cannot block the perfusion circulation main path, the perfusion circulation main path can still continue to work at the moment, the in-vitro organs can be continuously protected, and the leukocyte filter on the filtering circulation auxiliary path can be replaced in time.

The invention provides a microfluidic chip for studying the tumor single cell invasion and epithelial-mesenchymal transition process capability. The microfluidic chip includes four layers of structures that are stacked together in order and sealed to each other, and falls into a top cover, a microfluidic channel layer, a substrate with micropore array and a baseplate with a cavity group from top to bottom. The invention also provides a system that matches with the chip and is used for single cell detection. The microfluidic chip and the corresponding detection system provided by the invention can achieve rapid, simple and low-cost sorting of single cells and study of invasion ability and epithelial-mesenchymal transition, and performs gene analysis on a target single cell.

The invention discloses a simulation experiment device for coal mine gas dynamic disaster under a simulation environment. The device comprises a loading frame, wherein a pneumatic hammer and two Z direction loading hydraulic cylinders are mounted on the loading frame, and a Z direction loading plate is connected between the bottom ends of piston rods of the two Z direction loading hydraulic cylinders; a Y direction guide rail and an X direction guide rail are connected to the bottom of the loading frame; a protruding cylinder body and a Y direction loading hydraulic cylinder are arranged on the Y direction guide rail, and an X direction loading hydraulic cylinder is arranged on an X direction motor trolley; a Z direction loading port is formed in the top of the protruding cylinder body, a Y direction cylinder and an X direction cylinder are connected to the side wall of the protruding cylinder body, and a protruding port is formed in the upper surface of the X direction cylinder; a fourth pipeline with an opened tail end is connected to the X direction cylinder; a vacuum-pumping system and a gas loading system are connected to the protruding cylinder body. The invention further discloses an experiment method using the device, coal mine rock burst, gas flow field, overlaying strata stress and horizontal tectonic stress can be simulated, the experiment simulation degree is improved, and the experiment success rate and the accurate degree are improved.

The invention provides a multifunctional integrated microfluidic chip for conducting online screening of multiple types of cancer cells simultaneously and belongs to the technical field of microfluidics. By means of the multifunctional integrated microfluidic chip, the experiment of conducting online screening of multiple types of cancer cells simultaneously can be completed, and 16 types of cancer cells can be inoculated; online screening of multiple types of medicine simultaneously can be achieved, and online screening of eight types of cancer-resistant medicine simultaneously can be completed. The multifunctional integrated microfluidic chip comprises three layers, namely a first PDMS film layer, a second PDMS film layer and a glass bottom layer, and the three parts are bonded by irreversible plasms from top to bottom in sequence to form a composite integrated structure. Four modules of the same structure and a waste liquid opening are integrated into a chip body, four columns of cell culture channels, a fluid channel, a cell injection opening, two medicine injection openings and six micro valves are integrated into each module, a bent pipe communicated with the cell culture channels is arranged below each column of cell culture channels, and the top end of each bent pipe is provided with an outlet.

The invention discloses a detection method for biomolecular probe-calibrated specific sites of DNA based on a nanopore device and application thereof. The method comprises the following steps: 1) calibrating the specific sites of DNA by using a biomolecular probe so as to obtain calibrated DNA; and 2) detecting the specific sites of the calibrated DNA by using a nanopore sequencing device, wherein the calibrated DNA is added into a positive electrode chamber filled with an electrolyte, an external pressure field is introduced to the positive electrode chamber during detection and is used as an external driving field for DNA perforation, voltage is applied between a positive electrode and a negative electrode and used as an external electric field with a direction opposite to the direction of the external pressure field, and an active force of the external pressure field on the DNA in a nanopore during detection is greater than an electric field force. With the method provided by the invention, secondary current signals totally different from those of common unmodified DNA are obtained, the spatial positions and sequence of modified specific sites of the DNA can be characterized through analysis and statistics of the secondary current signals, so a physical map of the long chain DNA is obtained.

The invention discloses a micro-sample preparation device for a frozen electron microscope and a sample preparation method. The micro-sample preparation device comprises a supporting net assembly witha sample preparation operation state and a freezing operation state, a sample preparation probe used for respectively carrying out sampling operation and completing sample preparation operation on ato-be-detected sample solution on the supporting net assembly, a three-dimensional translation platform used for driving the sample preparation probe to be converted between the sampling operation andthe sample preparation operation, a freezing module used for carrying out freezing operation on the sample solution on the supporting net assembly, and a rapid transfer device used for driving the supporting net assembly to transfer between the sample preparation operation state and the freezing operation state. According to the invention, a frozen electron microscope sample preparation experiment with ultramicro sample consumption can be realized, and the consumption of a single sample liquid drop is in a nano-liter level and below the nano-liter level. Compared with a conventional sample preparation robot, the sample consumption is reduced by 1000 times or above, the experiment cost is remarkably reduced, and the protein sample purification period is shortened. The success rate of frozen electron microscope sample preparation and the reproducibility of experiments are obviously improved.

The invention relates to a split type miniature peristaltic pump, in particular to a miniature peristaltic pump, wherein a pipeline is integrated to a pump head, and both the pump head and the pipeline can be used for one time. The micropump comprises a pump body, a motor and a pump head and pipeline assembly. The pump head and pipeline assembly comprises a pump head seat, an eccentric wheel, a pump head roller and a circumferential pressing pipeline; a pump head seat center hole is formed in the pump head seat, a through hole is coaxially formed in the bottom of the pump head seat center hole, and a pipeline inlet / outlet groove is formed in one side of the pump head seat; the pump head roller is arranged in the pump head seat center hole, a pump head roller center hole is formed in the pump head roller, and the eccentric wheel is arranged in the pump head roller center hole and connected with an output shaft of the motor through an eccentric hole; and a pump head roller groove is formed in the outer wall of the pump head roller, and the circumferential pressing pipeline is sleeved with the pump head roller groove along the outer wall of the pump head roller. According to the micropump, the structure of the assembly formed by the pump head and the pipeline can guarantee that the pump is compact in structure and small in size; and the single-use purpose can be achieved, the pollution risk is lowered effectively, and the experiment success rate is increased to a great extent.

The invention discloses an experimental method for isothermal local loading formation of a titanium alloy. By adopting a terrace die to increase the stability of a die and a blank which are matched, the problem that a test sample and an arc-shaped die are easy to deviate to be unstabilized in the assembling process is solved, and the experimental success rate is remarkably increased. Inhomogeneous deformation of a local loading formation transition region is realized by way of loading in steps by using the terrace die and not using the terrace die. Die processing and experimental operation are simple, convenient and feasible and well reflect the deformation characteristic of the transitional region in local loading formation. According to the experimental method disclosed by the invention, the deformation gradient of the blank is more uniform, easy to control and convenient for subsequent tissue observation of a special deformation region compared with that of an arc-shaped die, and thus a good experimental foundation is provided for quantitative research which discloses microstructure development mechanism and rule in the transitional region in isothermal local loading formation of the titanium alloy.

The invention discloses a rigid and flexible two-stage locking ball joint. A two-stage piston is arranged in the ball joint; a two-stage locking state is realized; when the fluid pressure is not sufficient to simultaneously push the two-stage piston, the ball joint is in a flexible locking state; when the fluid pressure can simultaneously push the two-stage piston, the ball joint is in a rigid locking state. Through a connecting sleeve, the ball joint is fixed with the head end and the tail end of the sleeve to form a ball joint connecting rod; then, the two or a plurality of ball joint connecting rods are connected; a multi-freedom operation arm can be formed; a tool or an operation appliance can be positioned; when different pressure is exerted on each connecting rod, the locking state of the ball joint can be controlled so as to adapt to different joint loads.

The invention discloses an oil-water-gas production control and metering device and method in a heavy oil exploitation experiment, and relates to the physical simulation experiment field of heavy oilsteam injection SAGD exploitation. The device comprises an oil reservoir model, an output fluid regulating unit, an oil-gas-water separation unit and an oil-gas-water production and collection unit. The output fluid regulating unit comprises a temperature sensor used for measuring the temperature in the oil reservoir model, a pressure sensor used for measuring the pressure in the oil reservoir model and a first automatic regulating valve communicated with the oil reservoir model. The oil-gas-water separation unit comprises a heat exchange unit communicated with an automatic regulating valve, agas-liquid separation unit communicated with the heat exchange unit and a defoaming unit communicated with the gas-liquid separation unit. The oil-gas-water production and collection unit comprises afirst collection unit connected with the gas-liquid separation unit, a second collection unit connected with the fluid outlet of the defoaming unit and a gas metering unit communicated with the gas outlet of the defoaming unit. The gas metering unit is used for measuring the mass of gas flowing out of the defoaming unit. According to the oil-water-gas production control and metering device and method in the heavy oil exploitation experiment, the accuracy of the experiment can be greatly improved.

The invention discloses a brain buried pipe medicine applying cannula capable of recording cell external electric potential. The brain buried pipe medicine applying cannula capable of recording the cell external electric potential comprises a buried pipe cannula body, an electrode cannula, a pipe core cap, a medicine applying pipe, a fixing nut, a wire electrode, a pipe core and a buried pipe cannula cap. The brain buried pipe medicine applying cannula capable of recording the cell external electric potential combines the five functions of stimulation, damage, medicine application and recording as a whole, therefore, experimental operation is simplified, experimental cost is reduced, experimental success rate is improved, and the flexibility and accuracy of devices are improved. The brain buried pipe medicine applying cannula capable of recording the cell external electric potential is simple in structure, convenient to operate and low in manufacturing cost, and well solves the problems that an existing brain buried pipe medicine applying cannula is single in operating function and high in using cost and causes secondary damage easily.

The invention discloses a testing device and method for rock straight shear-seepage, and relates to a rock mechanics test technique. An involved three-axis compression shear testing device comprises an axial shear pressing head (1), a pressure chamber (3), sealing tape (4), a deformation rubber sleeve (5) for isolating contact between a test piece and oil, the testing test piece (6) with a prefabricated joint, and a rigid cylindrical base plate (8). According to the adopted testing method and test piece preparation method, the characteristics of a rock three-axis compression experiment are fully utilized, the stable confining pressure is provided for the test piece through the oil pressure chamber, the rock test piece with the prefabricated joint is prepared through the special method, meanwhile, the conditions for a shear test are created, and then a seepage test is carried out. By adopting the method to carry out the shear-seepage tests through a three-axis testing instrument, obtained data are more accurate and more reliable, and the shear damage condition of rock under the natural conditions is simulated to a larger extent.

The invention provides multifunctional heterogeneous core model equipment. The equipment comprises a core model main body, a top cover and an auxiliary tamping module; the auxiliary tamping module is used for tamping the core model main body filled with quartz sand; the top cover is used for closing an opening of the core model main body when the tamping is finished; inlets, outlets and threaded channels are formed on four walls of the core model main body; the inlets and the outlets are formed on the wide side wall of the core mode main body; the threaded channel is formed on the long side wall of the core model main bodies. By use of the equipment provided by the invention, the prepared core is large in volume, and the simulation effect on the displacement experiment is good; a regulating button at one side can fast modify the model permeability so as to increase the experiment success rate; and a pressure measuring point or a testing electrode can be installed at other side according to the experiment requirement, thereby acquiring the water saturation change in the displacement process, and expanding the function of the experimental core. The whole equipment is detachable, and can be reused.

The invention discloses a rat brain stereotaxic fitting device. A work table is provided with a first step surface and a second step surface from top to bottom; two upright posts are arranged at the position, along the width, on the first step surface; limit holders are fixedly connected to the positions, at the same height, of the same sides of the two upright posts; each limit holder is providedwith a through hole of which the axis direction parallel to the width of the first step surface; an ear bar is inserted in each through hole in a sliding adjustable manner; the opposite ends of the two ear bars are tip ends. An adjusting mechanism can realize sliding adjustment along the length of the second step surface and is connected with a front tooth rod; the front tooth rod comprises a horizontal plate and a vertical bar; the vertical bar is arranged on the adjusting mechanism and capable of sliding for adjustment along the vertical direction, one end of horizontal plate is fixedly connected to the top end of the vertical bar, the other end of the horizontal plate is provided with a front tooth hole and extends along a gap between the two upright posts. The fitting device is used for fixing rat's head for biological study tests.

The invention discloses a data analysis method based on biological data, which comprises the following steps: acquiring biological data, and establishing a sample database; Performing feature extraction processing on the biological data in the sample database to form a plurality of clusters with different attributes, and establishing a cluster library; Extracting data in the clustering library toestablish a high-dimensional model; Filtering the biological data needing to be subjected to the experiment through a high-dimensional model to obtain feasible data; According to the invention, the biological data is clustered; The technical problems that in the prior art, needed biological data cannot be intelligently extracted from mass data, and wrong data cannot be removed are solved by combining with the data model, so that the needed biological data can be intelligently extracted from mass big data, the wrong data can be removed, and the experiment success rate is increased; And the biological data is clustered, so that the class group of the required data can be quickly found from the mass data, and the working efficiency is improved.

The invention discloses a method and a device for charging air for a diamond butting pressure anvil. The air charging method comprises: making gas highly dense and even liquefied by a low-temperature cooling means; then making the gas enter sealing cavities among diamond pads in a cooled DAC device, and ensuring the gas fully fills the cavity at the pressure slightly higher than the atmospheric pressure; tightly locking the DAC device through external force; and sealing the gas to be charged into a sample cavity. The device for charging air for the diamond butting pressure anvil comprises a diamond butting pressure anvil body, a gas introducing device, a sealing device and a cooling device. The method can make air flow in the sample cavity more stable, can obviously improve experiment success rate and can greatly reduce unnecessary gas loss at the same time.

The invention discloses an automatic transmitting sample grinding robot which comprises a base, a support, a rotary platform, a rotary motor and the like. The support is installed on one side of the base. The rotary motor is installed in the center of the base. A main shaft of the rotary motor is arranged vertically upward. The rotary platform is coaxially mounted on the main shaft of the rotary motor. An abrasive paper clamping ring is mounted on the circumference of the rotary platform in a clamped mode. A cylinder body of an electric lifting cylinder is vertically mounted at the top of the support. A mounting table top rod is coaxially installed in the center of a sample mounting table. A water spray tube and a grinding fluid tube are mounted on the two sides of the lower portion of the support correspondingly. An outlet of the water spray tube and an outlet of the grinding fluid tube are located on the two sides of the rotary platform correspondingly. According to the automatic transmitting sample grinding robot, the disciform sample mounting table and the automatic electric lifting cylinder are arranged to achieve the purpose of automatic grinding of transmitting samples and improve the experiment efficiency and the success rate; and the automatic transmitting sample grinding robot is high in efficiency and convenient to operate.

The invention discloses a rotating shaft type variable-angle loading clamp based on a light-gas gun and a mounting method thereof, and belongs to the technical field of experimental equipment. Two target plate supports are fixed on a height adjusting connecting plate, target plate fastening blocks are arranged on the target plate supports, semicircular grooves are respectively formed in two opposite surfaces of the target plate fastening blocks and the target plate supports, adapter shafts are arranged in the semicircular grooves, the target plate fastening blocks and the target plate supports are fastened and connected through bolts to press the adapter shafts, and the adapter shafts are fixedly connected with connecting blocks. a connecting block is fixed on the rear side of a target plate rear clamp, and a target plate front clamp and the target plate rear clamp clamp a target plate; a target cabin connecting base is fixed at the bottom of a target cabin heavy frame, an incident plate is fixed on the front side of the target cabin heavy frame, the top and other three sides of the target cabin heavy frame are closed through protective plates, and a loading clamp is fixedly arranged on the target cabin connecting base; the separating flange is arranged in the incidence long hole of the incidence plate in a sliding mode, the separating flange is connected with the incidence plate, and a target projectile sample can pass through a center through hole of the separating flange. The rotating shaft type variable-angle loading clamp is suitable for variable-angle loading of the light-gas gun.

A Pickering emulsion is prepared by using a cellulose derivative which is one of ethyl cellulose, cellulose acetate, cellulose acetate propionate, cellulose acetate butyrate and acetylated nanocrystalline cellulose a solid emulsifier and using pollution-free edible oil as an oil phase, and the cellulose derivative has hydrophobic groups, so that the stability of the oil-in-water Pickering emulsionis facilitated. The acetylated modified nanocrystalline cellulose has strong interaction with an oil phase, so the stabilizing effect is good, the droplet size of the Pickering emulsion is significantly reduced, and the particle size distribution of the emulsion is uniform.

The invention discloses a piezoelectric ultrasonic microinjector which comprises a substrate, a screw cap and a piezoelectric ceramic vibrating module fixedly mounted at two ends of the substrate, anend cover fixed to the screw cap, bent plain end injection needles fixedly mounted on the screw cap and the end cover and a fixed bar fixedly mounted on the piezoelectric ceramic vibrating module. Thepiezoelectric ultrasonic microinjector disclosed by the invention is simple in structure, good in interchangeability of parts, easy to process and relatively low in processing cost, and the experimental cost of a microinjection experimental operation is lowered and the complex degree of the microinjection experimental operation is simplified.