Method for decelerating nucleic acid molecules in solid nanopore

A nucleic acid molecule and nanopore sequencing technology, which is applied in biochemical equipment and methods, analytical materials, and the determination/inspection of microorganisms. and improved controllability, high current signal-to-noise ratio, and improved time resolution

Active Publication Date: 2012-07-18
HARVARD UNIV +1
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Moreover, with the increase of glycerol, the viscosity coefficient of the solution increases, and the amplitude of the DNA perforation blocking current value decreases, so that the signal-to-noise ratio of the test decreases, resulting in an inc

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  • Method for decelerating nucleic acid molecules in solid nanopore
  • Method for decelerating nucleic acid molecules in solid nanopore
  • Method for decelerating nucleic acid molecules in solid nanopore

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[0026] Example,

[0027] 1) Preparation of chip devices

[0028] The purpose of this step is to prepare a solid-state nanoporous device that can be operated in a transmission electron microscope, so that the high-energy convergent electron beam in the transmission electron microscope can be used to punch holes in the suspended film of the device, thereby realizing a nanoporous device. This includes a series of micro-nano processing technology involved in traditional semiconductor processing technology, and also involves a series of modern cutting-edge nano-scale processing technology.

[0029] The specific method is as follows: First, a 4-inch silicon wafer with a (100) surface is grown with 2 microns of silicon oxide on both sides, and low-stress silicon nitride of about 150 to 200 nanometers is deposited by a low-pressure chemical vapor deposition method. Then make a photolithography mask, the purpose is to make a lot of 3mm×3mm small substrate periodic distribution on a 4-...

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Abstract

The invention discloses a method for reducing perforation speed of nucleic acid molecules in a nanopore sequencing method. The method comprises the following steps of: adding the nucleic acid molecules to be measured into a nanopore sequencing device filled with electrolyte, wherein the nanopore sequencing device comprises an electrolytic cell provided with an anode and a cathode, and a solid nanopore film for separating the anode from the cathode of the electrolytic cell; placing the nucleic acid molecules to be measured in a cathode cavity of the electrolytic cell,; and when the nucleic acid molecules are measured, applying the voltage between the anode and the cathode, and introducing a reverse external field taking a pressure intensity external field as an electric field simultaneously. According to the method, the pressure intensity external field is reversely applied under the drive of an additional electric field, so that the speed of DNA (Deoxyribonucleic Acid) molecules is reduced when the DNA molecules pass through the solid nanopore, and the speed is reduced by about 50% to 80%, thus the time resolution of a DNA unimolecule detection technology is greatly improved. The method has very bright application prospect on a solid nanopore DNA molecule sequencing device.

Description

technical field [0001] The invention relates to a method for decelerating nucleic acid molecules in a solid nanopore. Background technique [0002] DNA single-molecule detection and analysis based on solid-state nanopore devices is considered to be one of the most promising technical routes for realizing the third generation of rapid and low-cost human gene sequencing (realizing the genome sequencing of a single person within 24 hours at a cost of less than $1,000), and has become In addition to research groups such as Harvard University, Boston University, Brown University, Caltech, and Delft University of Technology in the Netherlands, IBM also announced in October 2009 to join the next generation of human genome sequencing- The $1,000 plan uses nanopore device-based sequencing technology as a realization method, enabling nanopore research to move from basic research to application (D.Branton, et al.Nature Biotechnology26(10), 1146(2008); M.Zwolak and M. Di Ventra, Review...

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Application Information

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IPC IPC(8): G01N27/403
CPCC12Q1/6869G01N33/48721C12Q2523/303C12Q2527/109C12Q2565/631
Inventor 鲁铂赵清俞大鹏
Owner HARVARD UNIV
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