160results about How to "Amplify" patented technology

The invention provides a micro fluidic chip for sorting and whole genome amplification of a single cell. The micro fluidic chip comprises a four-layer structure, wherein the four layers are stacked together in order and mutually sealed, and the structure comprises the following layers from top to bottom: a top cover, a micro valve control layer, a valve plate layer and a channel layer; and the bottom of the micro fluidic chip is externally provided with a magnet. The invention also provides a system for screening and identifying cells as well as amplification and analysis of a single cell, and the system is matched with the chip. The micro fluidic chip and the corresponding detection system can be used for sorting target cells from a large amount of cells quickly, simply and cheaply, and amplifying and analyzing whole genome DNA of a single cell therein.

The invention discloses a micro-fluidic chip and a detecting device suitable for PCR (polymerase chain reaction) or HRM (high resolution melting) detection analysis. A micro pipeline and an array reaction chamber base plate form a combined mould, a micro-fluidic base chip is generated by virtue of an injection molding method, the micro-fluidic chip is formed by virtue of plasma cleaning and glass bonding; a rotary rod with a handle and a duct is transversely embedded in an outward channel center of the chip to form a rotary valve; the handle rotates to disconnect the duct to control on-off and sealing of multi-reaction-chamber sampling, so that physical isolation between reaction chambers is realized, the sealing performance is good, and cross contamination does not exist, and thus, a plurality of samples can be detected at the same time. The micro-fluidic chip disclosed by the invention not only can be used for independent PCR detection or HRM detection, but also can be used for performing PCR detection and then performing HRM detection without any change in a combined manner, so that the multi-functionalization of detection is realized.

The invention belongs to the field of medical biological technique and discloses a method for digitally detecting micro-mutation by using a micro-emulsion clone amplified bound water gel microsphere chip. The method detects the micro-mutation by combining the microsphere mediated micro-emulsion clone amplifying technique with the water gel chip fixing microsphere fluorescence detection and digitization analysis techniques. The method promotes the positive relevance ratio of micro-mutation, has low cost, high sensitivity and fine specificity and is applied to quantitatively measuring micro-mutation of various low abundance tumor relative genes.

The invention belongs to the field of a genetic engineering, and relates to a loop-mediated isothermal amplification (LAMP) primer composition for detecting phytophthora rot and an application thereof. The primer composition comprises a forward inner primer FIP showed in SEQ ID NO.1, a backward inner primer BIP showed in SEQ ID NO.2, a forward outer primer F3 showed in SEQ ID NO.3, a backward outer primer B3 showed in SEQ ID NO.4, and a reverse annular primer LB showed in SEQ ID NO.5. The LAMP primer composition can be applied to detection or identification of phytophthora rot. The LAMP primer composition has the advantages of good sensibility for specificity, rapid amplification, high efficiency and simple identification in the application of detecting phytophthora rot.

A method is provided for generating noise in an interior and exterior of a motor vehicle in which a control unit is provided which can be activated and which is operatively connected to a noise generator. The noise generator is connected to at least one noise emitting device (3). The noise emitting device (3) is mounted underneath a hood of the motor vehicle, and is connected to the vehicle interior via at least one hose with at least one sound transmitting element. Sound waves are extracted from the noise emitting device (3) and are transmitted into the vehicle interior.

The invention relates to an optimal method for efficiently separating and expanding mesenchymal stem cells in umbilical cord blood in cell therapy. The fact that the mesenchymal stem cells with higher differentiative capacity and smaller immunological rejection are contained in the umbilical cord blood has been proved. The establishment of an umbilical cord blood bank provides a basis for achieving transplantation of autologous cells. However, since the content of the mesenchymal stem cells in the umbilical cord blood is very low (only 0.5 to 30 mesenchymal stem cells are contained in 1*108 mononuclear cells), how to efficiently separate and expand the mesenchymal stem cells becomes a problem hindering the transformation of the mesenchymal stem cells in the umbilical cord blood to the clinical application. At present, in most situations, the mesenchymal stem cells in the umbilical cord blood are separated in virtue of adsorption capacity of the mesenchymal stem cells in a culture vessel, but the success ratio is low, and the characteristics of stem cells are difficult to maintain in an expanding process. The method comprises the steps of coating the culture vessel with protein components, adding multiple growth factors in the culture vessel in a coordination manner, and building the expansion environment of the mesenchymal stem cells in the umbilical cord blood, so as to achieve the efficient separation and expansion of the mesenchymal stem cells.

The invention discloses an LAMP detection primer composition for phytophthora infestans and an LAMP detection kit and an LAMP detection method of the LAMP detection primer composition. The LAMP detection primer composition consists of a positive inner primer FIP, a reverse inner primer BIP, a positive outer primer F3, a reverse outer primer B3 and a reverse ring primer LB. The detection method disclosed by the invention is high in accuracy, high in specificity, convenient to operate and high in practicability, realizes constant-temperature multiplication, also provides a new technical platform for detection on the phytophthora infestans and can be used for performing high-sensitivity quick detection on the phytophthora infestans; meanwhile, pathogens can be identified at the initial stage of disease invasion, and the pathogens in field soil can be detected. The LAMP detection primer composition has an important significance for preventing and treating late blight of potatoes and tomatoes, reducing the blind use of pesticide, lowering the production cost and alleviating the environmental pollution of the pesticide.

The invention belongs to the automatic control technology field and relates to an adaptive fault tolerance control method for flexible liquid-filled satellite attitude based on a fault characteristic model. According to the method, when a system in under the condition of liquid shaking, flexible vibration and external interference, a fault tolerance controller based on the fault characteristic model is designed for performer faults. The method comprises steps that (1), the fault characteristic model is established according to a flexible liquid-filled satellite attitude kinetic equation; (2), a parameter estimation algorithm is utilized to identify a coefficient of the fault characteristic model, and a coefficient estimation value of the fault characteristic model is acquired; (3), the adaptive fault tolerance controller is designed according to the coefficient estimation value of the fault characteristic model; and (4), a control torque is acquired through the adaptive fault tolerance controller, and the control torque is applied to the satellite attitude control system to control a satellite attitude angle. Through the method, control precision before and after fault generation is guaranteed to be quite high, and the time for realizing the stable state is short.

The invention provides a human ALDH2 genotype detection kit comprising a PCR amplification primer, DNA polymerase and a reaction buffer solution; wherein the DNA polymerase is a polyethylene glycol (PEG) modified hot-start Taq polymerase which can tolerate the PCR reaction inhibitors in blood; and further provides the nucleotide sequence of the PCR amplification premier. The kit has the following advantages: (1) the DNA polymerase is a polyethylene glycol (PEG) modified hot-start Taq polymerase which can tolerate the PCR reaction inhibitors in blood, thus can realize the human whole-blood PCR amplification without carrying out nucleic acid extraction, and is capable of directly carrying out PCR amplification in blood; (2) the kit can carry out detection on a sample ALDH2 genotype.

The present invention provides a nucleic acid extraction, amplification and detection device. The nucleic acid extraction, amplification and detection device comprises a base seat, an extraction component and an amplification and detection component, the extraction component comprises a reagent rack detachably arranged on the base seat and a liquid suction mechanism for sucking a reagent, the reagent rack comprises a first main body, and reaction cavities and a plurality of reagent cavities arranged in the first main body, the reaction cavities respectively load an extraction reagent and a reaction reagent, the liquid suction mechanism is used for respectively sucking a sample, the extraction reagent and the reaction reagent and respectively transferring the sucked sample and reagents intothe reaction cavities; the amplification and detection component comprises an amplification and detection chamber, and the amplification and detection chamber is arranged on the base seat; and the amplification and detection chamber contains the reaction cavities for amplifying, detecting and analyzing the extracted sample. The reagent rack of the device can be detached and replaced, and different samples can be analyzed quickly; operation processes of nucleic acid extraction and a structure of the extraction component are simplified, and costs of the device and the operation are reduced; andthe nucleic acid extraction, amplification and detection device uses an integrated arrangement and is compact in structure, small in volume and convenient for carrying.

The invention discloses a method and a device for measuring an electric hysteresis loop and a strain loop of a ferroelectric material simultaneously; the measurement device comprises four parts: test signal output, electric hysteresis loop test, strain loop test and equipment control and data collecting treatment; waveform signals generated by a signal generator are amplified by a high voltage amplifier and are subsequently applied in a series loop consisting of a sample to be measured and a standard capacitor; the voltage signals at two ends of the standard capacitor are input to a collecting card by an impedance amplifier; simultaneously, the strain signal detected by an LVDT displacement sensor is input to a data collecting card; and the electric hysteresis loop and the strain loop are obtained by a computer. The method and the device solve the problem of no instruments for measuring the electric hysteresis loop and the strain loop simultaneously in the currently national ferroelectric piezoelectric material research. The method and the device have stable test data, high efficiency and good self-definition and extensibility and can greatly save the test time. The technical proposal has simple structural principle and is easy, convenient and practical for preparing and assembling devices.

The invention provides a human HLA-B27 gene typing kit. The human HLA-B27 gene typing kit comprises a PCR amplification primer, DNA polymerase and a reaction buffer solution, wherein the DNA polymerase is a hot initiated Taq polymerase which is modified by polyethylene glycol (PEG) and can tolerate PCR reaction inhibitors in blood. The invention also provides a nucleotide sequence of the PCR amplification primer. The human HLA-B27 gene typing kit has the technical effects that the adopted DNA polymerase is the hot initiated Taq polymerase which is modified by PEG and can tolerate PCR reaction inhibitors, PCR amplification on whole blood of human (nucleic acid extraction does not need to be carried out) can be realized, PCR amplification is directly carried out by virtue of blood, and DNA extraction does not need to be carried out; the human HLA-B27 gene typing kit can realize HLA-B27 negative and positive identification on a sample; meanwhile, a gene subtype result of an HLA-B27 positive sample can be obtained, and 24 HLA-B27 gene subtypes and other gene subtypes of 13 HLAB sites can be detected by every eight holes (eight linked pipes).

The invention discloses a method for detecting nucleic acid based on nano micro-bead nucleic acid extraction in a single tube, which takes nano-beads as the platform and completes extraction, amplification and detection of the nucleic acid in a single reaction. In this way, it can integrates extraction, amplification and detection to be a continuous process to ensure the extracted nucleic acid to totally participates amplification and detection, and it facilitates the high-throughput nucleic acid detection experiment. The technical program include: it releases the nucleic acid in the sample and mixes with nano-beads. The nucleic acid and nano-beads reacts to achieve nucleic acid -nano-bead complex if there is nucleic acid in the samples. The nucleic acid - nano-bead complex separates with other ingredients. The separated nucleic acid - nano-bead complex is directly used for the real-time nucleic acid amplification of the target nucleic acid detection system to make it clear whether there is specific nucleic acid sequences in the samples and determines their concentrations.

The present invention relates to the field of cellular immunotherapy for tumors and, more particularly, to a recombinant plasmid for the construction of recombinant malaria parasites and their use, wherein the recombinant plasmid is a tumorigenin-specific antigen gene inserted into the pL0017 plasmid, Recombinant malaria parasite, comprising the recombinant plasmid. Compared with plasmid DNA and RNA vector, the recombinant malaria parasite can be multiplied by the proliferation of Plasmodium, which is beneficial to the increase of the antigen in vivo. Compared with the defective virus and bacterial carrier, it survives in the red blood cells of the body longer, not short-term by the body's immune system to clear, long-term effective expression of exogenous tumor antigen, is conducive to long-term antigen and immune stimulation. The recombinant malaria parasite is capable of activating the high expression of Th1-related cytokines in vivo and increasing the proportion of CD8a + DCs in total CD11c + DCs and further activating specific cytotoxic T lymphocyte responses against tumor antigens, which is beneficial to the antitumor effect of the vaccine.

Wavelength division multiplex millimeter wave light source row includes multiple wavelength comb type light signals generator, one optical demultiplexer, multiple multimode laser, and one multiplexer. The optical demultiplexer makes demultiplexing multiple wavelength comb type light signals. Multimode laser is input for each distributive wavelength segment. The multimode laser generates mode-locking light signals in dual mode with mode space in millimeter wave frequency. The multiplexer inputs mode-locking light signals in dual mode into optical fiber connected to base station after multiplexing. Under condition of shared microwave oscillator in frequency being as 1/N millimeter wave frequency and light signal modulator in same low frequency, the invention generates millimeter wave light source row by using harmonic modulation technique and mode locking dual mode technique. The invention makes cost for generating light signal in millimeter wave decrease greatly.

The invention discloses an electrochemical biosensor for detecting ampicillin and a preparation method thereof. The electrochemical biosensor includes an electrode, and nanogold, a probe DNA and an aptamer DNA that are sequentially modified on the surface of the electrode. A sequence of the probe DNA is shown as SEQ ID NO.1; and the sequence of the aptamer DNA is shown as SEQ ID NO.2. The electrochemical biosensor utilizes good biocompatibility and conductivity of the nanogold, so that amplification of a photoelectric signal is achieved, and the detection sensitivity of the ampicillin is improved. A detection method of the electrochemical biosensor is simple, an instrument is miniaturized, and an operation is easy; and the ampicillin can be detected only by simply processing the surface ofa glassy carbon electrode.

The invention discloses an LAMP detection primer composition for phytophthotacactorum, as well as an LAMP detection kit and an LAMP detection method of the LAMP detection primer composition. The LAMP detection primer composition consists of a positive inner primer FIP, a reverse inner primer BIP, a positive outer primer F3, a reverse outer primer B3 and a reverse loop primer LB; detailed sequences of all the primers are as follows: FIP: 5'-TCTGGGCACAACCGCAAAAA-TTTGCGAGCTCCAGATTTCC-3'; BIP: 5'-AATCCGTACGATCGAGCTGGAC-ACACGCCACGTCTGCT-3'; F3: 5'-TTCTGCGCTAGGCGACC-3'; B3: 5'-CACACAAGTGGACCGTTAG3'; LB: 5'-GGAAAGACCATCAAGCTCCAGAT-3'. The detection method disclosed by the invention is high in accuracy, high in specificity, convenient to operate and good in practicability; constant-temperature amplification is realized; meanwhile, a novel technology platform is provided for detection of the phytophthotacactorum; the detection method can be applied to high-sensitivity rapid detection of the phytophthotacactorum; simultaneously, pathogens can be identified in initial stage of invasion of diseases, and the phytophthotacactorum soil of a field can be detected. The LAMP detection primer composition for the phytophthotacactorum, as well as the LAMP detection kit and the LAMP detection method of the LAMP detection primer composition, disclosed by the invention, play an important role in epidemic treatment caused by the phytophthotacactorum, reduction of sightless usage of pesticides, reduction of production cost and reduction of environmental pollution caused by the pesticides as well.

An integrated and modular approach is provided to convert electrical power to ionic momentum and a resulting high differential voltage field potential using an integrated multi-planar or axial microwave array and energetically sympathetic dielectric antennae to convert distilled liquid water to steam plasma and propagate the resulting water derived ions and electrons, steam and microwave energy into a modular energetic planar arrangement for integration into reactor vessels of various designs for the reduction of waste stream and fossil sourced feedstocks into their fundamental gaseous components and simultaneous reformation into desired synthesis or methane gas. The formed steam plasma (initiating plasma) generates electrons and ions which are propagated differentially to create a high differential voltage field potential which in conjunction with dielectric heating and far infrared radiation induced feedstock ionic polarization effects creates a series of primary feedstock reducing plasma fields for efficient plasma gasification with simultaneous product gas reformation.

The invention provides a disposable portable closed device for nucleic acid extraction, enrichment and amplification detection, aiming at meeting the important demand of rapid portable nucleic acid detection at present. According to the invention, a lysis solution, a cleaning solution, an eluent and a nucleic acid amplification solution are pre-buried in different closed and isolated areas of the device, and then the device is simply rotated and extruded, so that related liquids sequentially pass through a nucleic acid adsorption area, sample lysis, nucleic acid purification and nucleic acid amplification detection can be realized step by step, and the requirements of 'sample feeding-result discharging' in on-site rapid nucleic acid detection are really met. Meanwhile, the device is a disposable closed device, so that the problem of cross contamination of amplification products can be effectively reduced and avoided.

The invention discloses Phytopythiumvexans detection primers, LAMP detection system, kit and method. The detection primers include a forward inner primer FIP, a backward inner primer BIP, a forward outer primer F3, a backward outer primer B3 and a forward loop primer LF. Compared with a traditional detection technology for identifying Phytopythiumvexans according to morphological characteristics,the detection method provided by the invention has the advantages of high accuracy and sensitivity and convenience in operation, and provides a new technical platform for Phytopythiumvexans detectionwhile realizing constant-temperature amplification. The method can be used for high-sensitivity rapid detection of the Phytopythiumvexans in production practice, pathogens can be identified in the early stage of disease infection, and the Phytopythiumvexans in field soil can be detected. According to the invention, reliable technical and theoretical bases are provided for prevention and treatmentof the Phytopythiumvexans.

The invention discloses a CPCR (convective polymerase chain reaction) amplification detection device based on chemical heating. The device comprises a chemical heating reactor, a thermal conduction module, a chemical heating pack, a thermal insulation casing, a CPCR tube and a paper strip detection chip; the thermal conduction module is heated by virtue of heat energy which is generated from a reaction between water and the chemical heating pack, so that the bottom of the CPCR reaction tube is heated, and the bottom of the CPCR tube is kept at 95+/-2 DEG C till the end of a CPCR amplification reaction; and after the reaction is finished, the CPCR tube is transferred to the paper strip detection chip and a detection result is judged and read by virtue of human eyes, so that detection is completed. According to the device provided by the invention, isothermal heating required by the CPCR amplification reaction is achieved by virtue of the heat energy generated from the chemical heating, and meanwhile, by judging and reading the detection result by virtue of the human eyes in the combination with paper strip detection, nucleic acid amplification and detection, without external electric power, can be achieve; therefore, a good foundation is laid for conducting accurate, convenient, rapid, low-cost and high-sensitivity disease diagnosis in such environments having no electric power or in lack of the electric power as field or outdoor environments, undeveloped or remote areas and the like.

A method for detecting blending of dairy cow milk into buffalo milk and buffalo milk dairy products by duplex polymerase chain reaction includes the steps of PCR (polymerase chain reaction) amplification of buffalo milk genome DNA (deoxyribonucleic acid) samples to be detected and electrophoretic analysis on PCR products, three primers C1, B1 and B2 are adopted for forming two pairs of PCR reaction, and the primer design scheme of a PCR system includes: the primer C1 and the primer B1 form a pair of primers for amplification of buffalo specificity gene segments, and amplification products are the buffalo specificity gene segments; and the primer C1 and the primer B2 form a pair of primers for amplification of dairy cow specificity gene segments, and amplification products are the dairy cow specificity gene segments. The method has the advantages of rapidness, accuracy and sensitivity and can complete detection by one-time PCR amplification.

The invention relates to the technical field of cell amplification culture and discloses a method for efficient in-vitro amplification of peripheral blood NK cells. The method comprises the followingsteps: 1) performing anticoagulation on whole blood by heparin sodium, and diluting by PBS; 2) spreading the diluted anticoagulant whole blood on the liquid level of a lymphocyte separation liquid; 3)centrifuging, absorbing a white film layer, blowing, beating and uniformly mixing the white film layer, centrifuging, discarding the supernatant and repeatedly washing; 4) removing CD3+ lymphocytes,dyeing peripheral blood monocyte antibodies and sorting the cells; and 5) diluting the CD3+ lymphocytes, adding IL-2, IL-15, IL-21 and L-7 and performing amplification. The CD3+ cells are removed before amplification, so that the cellular immunogenicity is greatly reduced; meanwhile, four specific cell factors are used according to the specific proportion to activate and amplify the NK cells, so that the amplification multiple of the NK cells is guaranteed and the safety of the NK cells during treatment is greatly improved.

The invention discloses an LAMP (loop-mediated isothermal amplification) detection primer composition, an LAMP detection kit and an LAMP detection method for P.tentaculata. The LAMP detection primer composition consists of a forward inner primer FIP, a reverse inner primer BIP, a forward outer primer F3, a reverse outer primer B3 and a reverse loop primer LB. The detection method disclosed by the invention is high in accuracy, strong in specificity, convenient to operate and good in practicability, can be used for quickly and conveniently detecting the P.tentaculata with high efficiency, high specificity and high sensitivity under an isothermal condition of 64 DEG C without complex instruments, can well satisfy field detection of the P.tentaculata, provides a new technical platform for the detection of the P.tentaculata, can be used for high-sensitivity quick detection of the P.tentaculata, and can also be used for early diagnosis of epidemic diseases and monitoring of germs in pathogenetic fields.