Method for digitally detecting micro-mutation by using micro-emulsion clone amplified bound water gel microsphere chip

Abstract

The invention belongs to the field of medical biological technique and discloses a method for digitally detecting micro-mutation by using a micro-emulsion clone amplified bound water gel microsphere chip. The method detects the micro-mutation by combining the microsphere mediated micro-emulsion clone amplifying technique with the water gel chip fixing microsphere fluorescence detection and digitization analysis techniques. The method promotes the positive relevance ratio of micro-mutation, has low cost, high sensitivity and fine specificity and is applied to quantitatively measuring micro-mutation of various low abundance tumor relative genes.

Description

technical field

[0001] The method belongs to the field of medical biotechnology, and relates to a microemulsion clonal amplification combined with a hydrogel microsphere chip digital detection method for micromutations. Background technique

[0002] In recent years, micromutation detection has played an increasingly important role in the early diagnosis of diseases and has become an important means of disease diagnosis. In the early stage of the disease, the number of mutants in related genes is far less than that of normal DNA molecules, making it difficult to detect micromutants. Among some existing techniques for detecting micromutations, DNA sequencing is a relatively classic method, but this method cannot detect samples with less than 20% mutations. However, usually only a very small part of the DNA has mutations in the early stage of cancer. In order to detect this very few mutations, many methods have been developed. Currently, the methods used for micro-mutation det...

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