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DNA methylation qPCR kit for cervical cancer detection as well as use method and application thereof

A methylation and kit technology, which is applied to a DNA methylation qPCR kit for cervical cancer detection and its use and application fields, can solve the problems of low specificity and sensitivity of detection methods, and achieve high sensitivity and specificity. High performance, avoid waste effect

Pending Publication Date: 2020-08-18
深圳市新合生物医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a multi-gene combined detection primer probe combination kit for early diagnosis, detection or screening of cervical cancer, so as to solve the defects of low specificity and sensitivity of detection methods in the prior art

Method used

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  • DNA methylation qPCR kit for cervical cancer detection as well as use method and application thereof
  • DNA methylation qPCR kit for cervical cancer detection as well as use method and application thereof
  • DNA methylation qPCR kit for cervical cancer detection as well as use method and application thereof

Examples

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Effect test

Embodiment 1

[0054] Example 1: Using the kit of the present invention to perform multiple qPCR detection of cervical cancer methylation on samples from non-cervical cancer control populations

[0055] (1) Experimental materials

[0056] 1. 10 cervical smears of non-cervical cancer control population;

[0057] 2. Tissue Genomic DNA Extraction Kit (purchased from Beijing Tiangen Biochemical Technology Co., Ltd.);

[0058] 3. EZ DNA Methylation-Lightning Kit (purchased from Zymo Research);

[0059] 4. The kit of the present invention (which includes combination 1 primers and probe combinations, ACTB amplification primers and probes for detection targets EPB41L3, JAM3 and PAX1);

[0060] 5. AmpliTaq Gold TM DNA polymerase and buffer reagents (purchased from Thermo Fisher).

[0061] (2) Experimental method

[0062] 1. Cervical tissue, and cervical cell brush DNA extraction

[0063] Take 1.0mL of cervical brush samples from non-cervical cancer control population confirmed clinically and pa...

Embodiment 2

[0076] Example 2: Using the kit of the present invention to perform multiple qPCR detection of cervical cancer methylation on DNA samples from cervical cancer patients

[0077] (1) Experimental materials

[0078] 1. 33 cases of cervical cancer patients with cervical brush;

[0079] 2. Tissue Genomic DNA Extraction Kit (purchased from Beijing Tiangen Biochemical Technology Co., Ltd.);

[0080] 3. EZ DNA Methylation-Lightning Kit (purchased from Zymo Research);

[0081] 4. The kit of the present invention (which includes a combination of 1 primer and probe combination, ACTB amplification primer and probe for detection targets EPB41L3, JAM3 and PAX1);

[0082] 5. AmpliTaq Gold TM DNA polymerase and buffer reagents (purchased from Thermo Fisher).

[0083] (2) Experimental method

[0084] 1. Cervical tissue, and cervical cell brush DNA extraction

[0085] Take 1.0mL of cervical brush samples from clinically confirmed pathologically confirmed cervical cancer patients into 1.5m...

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Abstract

The invention discloses a DNA methylation qPCR kit for cervical cancer detection as well as a use method and an application thereof. The kit is used for screening and diagnosing cervical cancer by detecting or measuring the methylation state or level of one or more specific genes in DNA of a tested sample. Through experimental tests, the method has the characteristics of quickness, high sensitivity, high specificity and the like, and can be used for diagnosing the human cervical cancer in time in the early stage, so that the early accurate diagnosis of the cervical cancer becomes possible, andthe waste of medical resources is avoided. The kit provided by the invention can significantly improve the positive detection rate and specificity (real negative rate) of early cervical cancer.

Description

technical field [0001] The invention belongs to the technical field of biotechnology and DNA detection, and in particular relates to a DNA methylation qPCR kit for cervical cancer detection and its use method and application, in particular to a DNA methylation method using cervical cell DNA qPCR is used to determine the methylation state of a target gene target for cervical cancer detection or screening kit and its use method. In addition, the present invention also relates to the application of the detection kit in biomedicine. Background technique [0002] Cervical cancer is one of the most common cancers in women, mostly occurring at the squamocolumnar junction (transition zone) of the cervix. Globally, there were an estimated 570,000 new cases in 2018, accounting for 7.5% of all cancer deaths in women. In China, cervical cancer cases account for more than 28% of the world's total, and the high-incidence age is 50-55 years old. In recent years, the incidence and mortali...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6827C12N15/11
CPCC12Q1/6886C12Q1/6827C12Q2600/154C12Q2523/125C12Q2531/113C12Q2561/101
Inventor 王志强万季蔡雪儿罗海燕王一杏夏迪汪健金泰庆关建洪王奕宋麒
Owner 深圳市新合生物医疗科技有限公司
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