42 results about "Mycoplasma hominis" patented technology

The invention relates to a real-time PCR (polymerase chain reaction) method for quintuply detecting target nucleic acid in a nucleic acid extracting solution in a single PCR reaction vessel, which is used for detecting Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium in a sample.

The invention relates to a kit for detecting mycoplasma hominis nucleic acid through a PCR-fluorescence probe method and a detecting method of the kit. The kit comprises a nucleic acid extracting set and a nucleic acid amplification detecting set. The nucleic acid amplification detecting set comprises PCR reaction liquid, negative control and positive control. The PCR reaction liquid comprises DNA polymerase, UNG enzymes, reaction buffer liquid, a primer, a probe and Mg2+. The positive control comprises positive plasmids and human genomes. The negative control comprises human genomes. The probe is a Taqman fluorescence probe. The special primer probe is designed according to an MH polymerase conservative target sequence by means of the kit and the method, the MH is rapidly detected through the PCR-fluorescence probe method, and the result is more reliable and accurate. Compared with an existing culture method, operation is easy and convenient and the speed is fast when mycoplasma hominis is identified; the whole process is completed within 3 hours; specificity and flexibility are high, and the result is visual.

Disclosed are a DNA chip and a kit capable of quickly and accurately detecting or genotyping the highly prevalent and important eleven microbes causing sexually transmitted diseases (STD) Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, syphilis-causing treponema, pallidum, chancroid-causing Haemophilus ducreyi, genital herpes-causing herpes simplex virus 1 and 2, human papillomavirus (HPV) and Trichomonas vaginalis and three related organisms Candida albicans, Gardnerella vaginalis and coliform bacteria and analyzing antibiotic resistance against tetracycline and lactam antibiotics, and a method for detecting or genotyping using the same. According to the present invention, the presence, genotype and antibiotic resistance of the fourteen organisms can be analyzed quickly and accurately from a DNA sample. With excellent sensitivity, specificity, reproducibility and accuracy of the 14 STD-causing and related microorganisms may be automatically identified quickly and accurately from multiple samples, and selection of antibiotics may be aided.

The invention relates to a preparation method for multiple mycoplasma monoclonal antibodies and a multi-linked immunoassay reagent. The preparation method is characterized by separately immunizing mice with mycoplasma pneumoniae, mycoplasma hominis and ureaplasma urealyticum for cell fusion and cloning to obtain three monoclonal antibodies with high specificity and high potency. A mycoplasma multi-linked immunoassay reagent is prepared by mixing the three monoclonal antibodies according to a certain ratio, is used for detecting the diseases infected by the three mycoplasmas, and can quickly and accurately detect mycoplasma diseases; and sampling without phlebotomizing does not bring pain to patients, thus providing patients with timely and effective treatment. The multi-linked immunoassay reagent does not generate harmful waste gas or waste water during preparation and use processes, and also does not bring any injury to patients and operators, thereby being an environment-friendly, safe, accurate and sensitive biological preparation.

The invention provides a gene chip used for detecting the common pathogens of sexually transmitted diseases and a kit used for detection, wherein, the gene chip includes a solid phase vector and an oligonucleotide probe fixed on the solid phase vector; the oligonucleotide probe mainly comprises a DNA segment or a complementary DNA segment thereof which is selected from Diplococcus gonorrhoeae, Ureaplasma urealyticum and the 16S rRNA gene of M.hominis, the outer membrane protein gene (ompA gene) of a Chlamydia trachomatis, the glycosidoprotein B gene (gB gene) of a herpes simplex virus (HSV) and the L1 gene of a papilloma virus (HPV). The gene chip and the kit can be utilized for achieving the goal of detecting the common pathogens of sexually transmitted diseases; the gene chip and the kit for detection are simple and convenient to be operated, have high flux, accuracy and repetitiveness; and the gene chip and the kit for detection can be applied to the clinic detection and epidemiology analyzing of medical and health organizations.

The invention relates to the technical field of medical biology, and especially relates to a pathogen nucleic acid-drug resistant gene detection kit and an application technology thereof. The kit designed by the invention includes the following two kinds of compositions: a PCR reaction buffer solution, an enzyme mixed solution, and a chlamydia trachomatis/mycoplasma urealytium/mycoplasma hominis/drug resistant gene multiple reaction solution; or a PCR reaction buffer solution, an enzyme mixed solution, a chlamydia trachomatis/mycoplasma urealytium/mycoplasma hominis multiple reaction solution, and a drug resistant gene reaction liquid. The kit adopts a multi-fluorescence quantitative PCR technology, can simultaneously detect multiple target genes in a same PCR reaction tube, and provides a powerful technical support for simultaneous detection of pathogens and drug resistant genes. A method has the characteristics of less required amount on samples, low cost, simple and convenient operation, high sensitivity and good specificity, and has extremely great social and economic significances.

The invention provides a pepper extract with a function of resisting infection of ureaplasma urealyticum and mycoplasma hominis, relating to a pepper extract and aiming at solving the problems that the drug resistance of the ureaplasma urealyticum and the mycoplasma hominis to antibiotic is increased to cause poor treatment effect. The pepper extract is obtained by extraction through the following method: pepper is ground into powder, the powder is put into a supercritical CO2 extraction kettle, the extraction temperature is 30-55 DEG C, the separating temperature is 25-45 DEG C, the extraction pressure is 15-35MPa, the separation pressure is 4-6MPa, the flow of CO2 is 4-12, and the extraction time is 1-2 hours, and then the pepper extract is obtained. The pepper extract is non-toxic, has the functions of resisting the infection of ureaplasma urealyticum and mycoplasma hominis and resisting the infection of drug-resistant ureaplasma urealyticum and drug-resistant mycoplasma hominis, and is applied in the field of resisting the infection of mycoplasma.

The invention relates to a urogenital tract infection pathogen multi-detection primer group and a detection device comprising the same. The primer group comprises a bottom piece and a cover piece, a first reagent storage groove, a second reagent storage groove and a connection passage are arranged on the bottom piece, the tail end of the second reagent storage groove is connected with an equalization passage, an exhaust system is arranged on the inner side of the equalization passage, and the first reagent storage groove, the connection passage, the second reagent storage groove and the equalization passage are connected with the exhaust system; equalization holes are formed along the equalization passage, and a sealing hole, a second buffer hole and a detection hole which are connected are formed in the radial direction of the equalization holes. One or multiple of Chlamydia trachomatis primer group, a gonococcus primer group, a ureaplasma urealyticum primer group, mycoplasma hominisprimer group and mycoplasma genitalium primer group and an internal reference primer group are contained in the detection holes respectively; an amplification reagent is contained in the second reagent storage groove or the detection holes. By the urogenital tract infection pathogen multi-detection primer group and the detection device, multi urogenital tract infection pathogen detection can be performed, single-sample multi-index simultaneous detection can be realized, and detection flux is increased.

The invention discloses a mycoplasma hominis gold mark quick detection kit and belongs to the field of clinical medicine detection. The kit consists of a clamping box and a test strip, wherein the clamping box comprises a box cover (4) and a box bottom (7); the detection test strip is arranged in the clamping box; a sample feeding hole (1) and an inspection window (2) are formed in the front side of the clamping box; a sample feeding hole tag (5), a result distinguishing illustration(6) and an information recording column (3) are printed on the front side of the box cover. According to the invention, the gold immunochromatographic assay technology is adopted, the engineering expression mycoplasma hominis Lmp1 protein is taken as an antigen to prepare a monoclonal antibody, the pair of high-performance mycoplasma hominis Lmp1 monoclonal antibodies with favorable pairing property are screened to respectively serve as a capture antibody and a detection antibody, and the mycoplasma hominis in secretion of the human body urogenital tract can be detected. The mycoplasma hominis gold mark quick detection kit has the advantages that the detection method is simple, the result display is accurate and quick, and special instrument equipment are not required.

The invention discloses a kit for detecting mycoplasma urealytium and human mycoplasma. The kit comprises four detection reagents: a compound of L-proline and enzyme substrate as chromogen for detecting activity of proline aminopeptidase, a compound of L-leucine and enzyme substrate as chromogen for detecting activity of leucine aminopeptidase, a compound of alpha-D-glucoside and enzyme substrate as chromogen for detecting activity of alpha-glucosidase and a compound of neuraminic acid and enzyme substrate as chromogen for detecting activity of neuraminidase, wherein the chromogen is aniline, naphthaline, naphthol, indoxyl and derivative thereof. According to the invention, a dry chemical enzyme method is adopted for detecting the enzyme compounded in the growth process or the enzyme generated by stimulating host cells, so that the purpose of detecting and identifying mycoplasma urealytium and human mycoplasma can be achieved; and the kit has the advantages of high speed, convenience, accuracy, and the like.

Provided is a ureaplasma urealyticum/mycoplasma hominis combined rapid culture and drug sensitivity detection kit. The kit can complete detection in 8-24 h, and meanwhile, drug-resistant detection of various antibiotics can be performed. The combined rapid culture kit is mainly composed of pork stomach digestive juice or lung digestive juice, a beef extracting solution or a beef heart extracting solution, cattle serum or horse serum or other animal serum, sodium chloride, peptone, yeast powder, urea, L-arginine, phenol red indicator or cresol red indicator, and penicillin or other antibiotics. The drug detection kit can detect doxycycline hyclate, roxithromycin, acetylspiramycin, gatifloxacin, clarithromycin, azithromycin, clindamycin, erythromycin, kitasamycin, ofloxacin, levofloxacin hydrochloride, sparfloxacin, ciprofloxacin, doxycycline, erythromycin cydocarbonate and various other antibiotics. By means of the detection kit, not only is the mycoplasma detection time shortened, but also drug-resistant detection can be performed at the same time so that clinical medication can be guided. The detection kit has the beneficial effects of being high in sensitivity, high in specificity, high in detection rate, easy and convenient to operate and the like.

The invention discloses a mycoplasma hominis detection system based on RPA-CRISPR/Cas12a and application of the mycoplasma hominis detection system, and relates to the field of microbiological detection. The detection system comprises an RPA (recombinase polymerase amplification) primer pair, a probe, crRNA (complementary Ribonucleic Acid) and AsCas12a protein, the sequences of the RPA amplification primer pair are as shown in SEQ ID NO: 3 and SEQ ID NO: 4; the sequence of the probe is as shown in SEQ ID NO: 16; the crRNA is a sequence as shown in any one of SEQ ID NO: 10 to SEQ ID NO: 12. According to the three rapid nucleic acid detection methods for the mycoplasma hominis, which are constructed on the basis of the RPA-CRISPR/Cas12a detection system, the detection sensitivity is increased, and the method disclosed by the invention shows extremely high specificity, so that a new technical platform is provided for the detection of the mycoplasma hominis.

The invention provides a LAMP detection method and a kit for mycoplasma hominis. 4 or 6 specific primers are designed for 6 or 8 regions of a gap genes sequence gene of the mycoplasma hominis, a LAMPdetection primer group is obtained through creative labor design of an inventor, is used for detecting the mycoplasma hominis in a sample and has the characteristics of rapidness, accuracy, simplicity, cheapness, repeatability, good specificity and high sensitivity. The detection line reached 42 pg per microliter.

The invention relates to a detection system capable of curing sexually transmitted infection pathogens and a kit and application thereof. The detection system comprises six pairs of primers for detecting treponema pallidum, trichomonad vaginalis, chlamydia trachomatis, neisseria gonorrhoeae, ureaplasma urealyticum and mycoplasma hominis, one pair of human DNA internal reference primers and one pair of system quality control internal reference primers. According to the detection system capable of curing the sexually transmitted infection pathogens and the kit thereof, the steps of conventional culture and the like are not needed, synchronous identification and semi-quantitative analysis of six pathogens difficult to culture can be directly carried out on a genital tract secretion sample in the same reaction system, and the detection system and the kit have the advantages of high specificity, high sensitivity, good repeatability, rapidness and accuracy; a comprehensive, accurate and low-cost etiological diagnosis basis can be provided for clinic and patients in the first time, and an important reference is provided for individualized medication and accurate medical treatment.

The invention discloses a camera-based automatic identification system for the pathogen culture test result. Steps are as follows, firstly, the software of the automatic identification system is opened; ureaplasma urealyticum and mycoplasma hominis culture and drug sensitivity detection reagent plates under the culture for 24 hours and for 48 hours can be placed by a user below a camera as needed,the camera is called by the software to collect images of the reagent plates; the ureaplasma urealyticum and mycoplasma hominis culture and drug susceptibility result is identified by the software according to the color of the specific position in the called image file; the result under the culture for 24 hours and for 48 hours is comprehensively analyzed by the software; a ureaplasma urealyticumand mycoplasma hominis culture and drug susceptibility result database is established by the software according to the called image file and the identification result, image acquisition and automaticresult identification of any ureaplasma urealyticum and mycoplasma hominis culture and drug sensitivity test reagent plate result can be carried out by the user, and image files and the identification result of reagent plates collected in the past can be checked at any time.