6results about How to "Simplify the experimental operation process" patented technology

The present application mainly relates to a kind of preparation of sulfur-rich amino acid protein CMd-copper nanoclusters and its detection mercury ion method, comprising: a) by prokaryotic expression obtains CMd protein, and the protein concentration of 5 mg / mL-25 mg / mL CMd protein solution is obtained by purification.b) CMd is reacted with reducing agent TCEP after opening protein disulfide bond.c) the protein solution after reduction is reacted with anhydrous copper sulfate, and preparation high fluorescence CMd-copper nanocluster.d) CMd-copper nanocluster is applied to detect heavy metal mercury ion.The application method can be synthesized under mild aqueous conditions by "one-step method" structure controllable, stable CMd-copper nanocluster, widens the raw material of copper nanocluster synthesized with protein as template, avoids the use of organic solvent and strong reducing agent, and reduces the cost and environmental burden brought by noble metal raw material, and also has higher selectivity to mercury ion.

The invention discloses a high-flux toxicity pre-evaluation substitution method based on an acetylcholin esterase target. By integrating an SH-SY5Y nerve cell endogenous AChE and recombinant exogenous AChE dual detection system and relying on an automatic screening system constructed by integrating core equipment such as an automatic pipetting workstation, a constant-temperature incubator and a multifunctional microplate reader, a high-flux toxicity pre-evaluation substitution method based on an AChE target is successfully established and is used for evaluating the neurotoxicity of a compound, and the method has the advantages of high sensitivity, high sensitivity, high sensitivity and the like. The method solves the limitation that a single screening method in the prior art cannot evaluate factors such as target inhibition, cell permeability and metabolic transformation at the same time, and the method is simple, convenient, rapid, high in accuracy and suitable for large-scale primary screening of the AChE inhibitor and early warning of neurotoxicity risks.

This invention discloses a computer program product and its application for data processing devices in the field of bioinformatics for ctDNA variant detection. The technical problem this invention aims to solve is how to detect ctDNA variants in early-stage cancer or postoperative minimal residual disease (MRD) under conditions of no UMI library construction and moderate sequencing depth. This invention constructs a set of supporting sequences for candidate variant sites, generating a sequence feature tensor and a fragment physical feature vector (including normalized fragment length). The former is input into a first neural network branch to extract sequence representation, and the latter into a second neural network branch to extract physical representation. A fusion module combines the sequence representation and physical representation, and a gating unit calculates the gating weight based on the physical representation and dynamically adjusts the contribution of the sequence representation, outputting the probability of the true ctDNA variant. This invention utilizes physical laws to suppress sequencing noise and can be applied to monitor MRD under conditions without molecular barcodes.

The utility model relates to the technical field of physical experiment teaching aids, in particular to a simple harmonic motion experiment instrument, which comprises a base, a liquid spraying device, a support arranged on the base and a paper feeding device arranged on the base, the support comprises a cross rod, a first vertical rod and a second vertical rod, the first vertical rod and the second vertical rod are arranged on two opposite sides of the cross rod, and the cross rod is provided with a cycloid and connected with a pendulum ball. A gap exists between the lowest point of the swing ball in the natural state and the base, a spring is fixed to the first vertical rod and is horizontally placed in the natural state, one end of the spring is detachably connected with the swing ball, the liquid spraying device comprises a spraying pipe, and the liquid outlet end of the spraying pipe is fixedly connected with the swing ball; a spring oscillator experiment and a simple pendulum experiment are integrated on one device, so that students can conveniently compare two simple harmonic motion phenomena on the same platform, the essence and generality of the simple harmonic motion can be deeply understood, and the teaching effect is optimized. Meanwhile, through the detachable connection design, quick switching between a spring oscillator experiment and a simple pendulum experiment is facilitated, and different experiment requirements are met.

The application relates to the technical field of magnetism measuring instruments, in particular to a magnetic sub-space imaging scanning device and method based on the magneto-optical Kerr effect. The device comprises a transmitting assembly, a scanning assembly and an imaging assembly. The transmitting assembly comprises a laser, a beam expander, a beam splitter, a mirror, a first half-wave plate, a polarization beam splitting cube, a calcite polarizer, a first dichroic mirror, an objective lens, an LED light emitting component, a convex lens and an achromatic doublet lens, so as to focus an incident light beam on a to-be-measured object. The scanning assembly comprises a second dichroic mirror, a second half-wave plate, a mirror, a beam splitting prism, a right-angle reflecting prism, an achromatic doublet lens, a photoelectric balance detector, a lock-in amplifier, a magnetic field applying component and a piezoelectric displacement stage, so as to scan the to-be-measured object. The imaging assembly comprises a convex lens, a CMOS camera and a data processing component, so as to generate a topographic image and a spin wave image. The application can perform high spatial resolution, high magnetic sensitivity and wide frequency band spatial imaging measurement on spin waves and magnetic sub-frequency combs.

The application relates to an organ chip clamp and an organ chip device. The organ chip clamp comprises a base and a cover plate. The base is provided with a placement area for placing a chip body, and the placement area is provided with a first pneumatic interface for communicating with a first gas inlet and outlet of the chip body; the first pneumatic interface is used for being connected to a gas pressure vacuum controller. The cover plate is connected with the base and is used for abutting to one side of the chip body away from the base, the cover plate is provided with a first sealing piece for sealing a liquid inlet and outlet of the chip body, and the cover plate is also provided with a second pneumatic interface for communicating with a second gas inlet and outlet of the chip body. The second pneumatic interface is used for being connected to the gas pressure vacuum controller. The organ chip clamp and the organ chip device can reduce experimental result error.