205results about How to "Simplify the experimental operation process" patented technology

The invention discloses a method for synthesizing a ZIF-8 aerogel on the basis of a cellulose hard template. The method is based on a cellulose hard template and comprises the following steps: reacting 2-methylimidazole and zinc nitrate to form ZIF-8 carried on the cellulose surface, carrying out solvent washing and repeated carrying so that the ZIF-8 crystal grains uniformly grow on the cellulose surface, and finally, carrying out exchange, freeze-drying and the like to keep the aerogel structure. The ZIF-8 aerogel has larger specific area, and has certain promoting actions on researching application of metal organic frame materials in the field of gas storage. The method has the advantages of simple technique and favorable repetitiveness, uses cheap and accessible industrial raw materials, conforms to the requirements for environmental protection, and can implement mass production.

The invention relates to a two-step quadrature phase-shift interferometry-based optical image encryption device and a method, which belong to the optical image processing technical field. The two-step quadrature phase-shift interferometry-based optical image encryption device comprises an optical image encryption device and an optical image decryption device. Due to the adoption of the two-step quadrature phase-shift interferometry-based optical image encryption device, strength information of substance light waves and reference light waves are not required to record, so the calculation amount and the storage amount can be reduced, the transmission efficiency of the information is improved, the system is simplified, and the cost is reduced. Since only two holographs are required to record, the experimental operation steps can be simplified by reducing the recording times. Since the charge coupled device (CCD) recorded digital hologram has advantages of real-time recording, digital memory, digital transmission and the like, encrypted information can be transmitted to an electronic computer of the decryption device to be decrypted in an electronic way to reappear, and the optical encryption technology can be better compatible with the digital signal processing and communication system so as to be widely used. In addition, system parameter key is also increased while the security of a double random phase coding method is guaranteed, so the security of the system is further improved.

The invention discloses a method for constructing lichi core collection, which comprises the following steps: selecting tender lichi leaves, and extracting genome DNAs according to varieties to obtain genome DNA samples of lichi; searching EST-SSR (Expresses Sequence Tag-Simple Sequence Repeat) of the lichi according to an EST sequence of the lichi, and designing an EST-SSR primer; carrying out PCR (Polymerase Chain Reaction) by using the EST-SSR primer to amplify the genome DNAs; carrying out electrophoretic analysis on PCR products to obtain existence data of alleles of the PCR products; and analyzing the obtained data to construct the core collection. In the method disclosed by the invention, the processes of EST-SSR-marked primer formation and experiment operation are simple and are easy to operate, an amplification band is clear, results are stable, and the method is suitable for analyzing genetic diversity among lichi resources, the core collection constructed on the basis almost represents all the genetic diversity of the original germplasm and has better representativeness, and the method has realistic significances for lichi germplasm management, new germplasm collection and germplasm reproduction and renewal and has great importance for promoting the exploration in the utilization of the lichi germplasm resources, so that the utilization efficiency of the lichi germplasm resources is improved.

The invention relates to a natural gas hydrate production monitoring simulation device and method. The natural gas hydrate production monitoring simulation device is characterized by comprising a hydrate reservoir forming subsystem for simulating the hydrate forming process, a producing well simulation subsystem for simulating the hydrate production process, a monitoring well simulation subsystemfor simulating the hydrate production monitoring process, a product collecting subsystem for conducting collecting treatment on products and a data processing and controlling subsystem for conductingdata collection and centralized control on the subsystems. According to the natural gas hydrate production monitoring simulation device and method, the processes of natural gas hydrate reservoir forming, hydrate production and monitoring and produced gas collection treatment can be simulated, real simulation of a hydrate in-situ environment is conducted, formation state change under various production methods is monitored by adopting a double-shaft monitoring mode, the experimental operation process of the natural gas hydrate production monitoring simulation device is simple, the safety is high, and experiments can be conducted repeatedly.

The invention discloses an improved sequence capturing method, which comprises the steps of 1, extracting DNA; breaking the DNA; 2, connecting the two ends of the broken DNA with a connector, whereinthe remote end of a connector sequence at one end of the broken DNA includes a tag sequence with the length being 6 to 8nt; 3, performing Pre-PCR on the DNA of the connecting connector; 4, mixing a probe and a sealing reagent; capturing the Pre-PCR, wherein the sealing reagent includes sealing sequences complementary with sequences except the broken DNA, and the tag sequences are sealed by C3 spacer arms with the same length; 5, after the hybrid capture, performing PCR amplification. A bridge type sealed design strategy is used; corresponding sealing sequences are respectively designed for connector sequences at two ends of inserting fragments; the tag sequences at the middle part are subjected to bridge type connection by the C3 spacer arms; the cost is reduced; the complexity in the library building process is simplified.

The invention discloses a water-phase preparation method of silicon quantum dots, comprising the following steps: (1) taking a reducing agent and a silane coupling agent and dissolving them in water under the protection of an inert gas, and the molar ratio of the silane coupling agent to the reducing agent is: 1:0.2~10; (2) Transfer the mixed solution obtained in step (1) into a high-pressure reactor and heat it to 140-220°C, and the silicon quantum dot solution can be obtained after the reaction; or the mixed solution obtained in step (1) The solution is transferred to a microwave reactor and heated to 140-220°C, and the silicon quantum dot solution can be obtained after the reaction; (3) the silicon quantum dot solution obtained in step (2) is mixed with an organic solvent, centrifuged, and the supernatant is removed. dry to obtain solid silicon quantum dots; wherein the reducing agent is citric acid, sodium sulfite, sodium borohydride, sodium citrate, ascorbic acid, urea, thiourea, hydrazine hydrate, L-cysteine, bovine serum albumin or Mixture of two or more in denatured bovine serum albumin.

The invention relates to the field of medical polymers, and discloses a preparation method of a controlled-release hydrogel with photo-thermal treatment and wound repair functions. The method comprises the following steps of: firstly, modifying dopamine on alginic acid to ensure that an o-diphenol side group is arranged on a molecular chain of the alginic acid, and simultaneously preparing coppersulfide @ melanin-PEG @ dopamine nanoparticles with good photo-thermal performance and radiotherapy effect; and then adding the copper sulfide @ dopamine nanoparticles into a dopamine-modified alginicacid solution, adding astragaloside with the function of promoting wound healing into the solution at the same time, and forming gel through a catalytic cross-linking system of horseradish peroxidaseand hydrogen peroxide. The hydrogel has good controlled release performance and can play a role in reducing administration dosage and reducing or avoiding toxic and side effects. The hydrogel prepared by the method has good biocompatibility, can be used for treating cancer cells by utilizing photo-thermal performance and radiotherapy, and can be combined with the astragaloside for promoting postoperative wound healing.

The invention provides an immunochromatography analysis detection system and a method. The system comprises a detection card and an immunochromatography analyzer; the detection card comprises a housing, immunochromatography detection test paper, and a two-dimension code; the immunochromatography detection test paper is disposed inside the housing, and the two-dimension code is disposed on the housing; the immunochromatography analyzer comprises a central processor, a card reader and a man-machine interaction device; the card reader is equipped with a card reading interface; the central processor is respectively connected with the card reader and the man-machine interaction device; additionally, the shape of the housing matches the shape of the card reading interface. The system of the invention can effectively simplify the flow of product detection, and improve the detection efficiency and accuracy.

The invention discloses a preparation method of tert-butyl (3R, 4S, 5S)-5-hydroxy-3-methyl-7-ocentyl-4-carbamate. The preparation method comprises the following steps: using N-tert-butoxycarbonyl-L-isoleucinal and allyl bromide as raw materials to prepare a crude product with a higher diastereoselectivity through a Barbier reaction; then performing column chromatography separation and purification to obtain the tert-butyl (3R, 4S, 5S)-5-hydroxy-3-methyl-7-ocentyl-4-carbamate. The synthetic product of the invention can be used as an intermediate for synthesizing a key synthon Dil of aplysiatoxin 10; the method of the invention is low in raw material cost, mild in reaction condition, short in reaction time, and easy to operate.

The invention provides a kit capable of detecting human serum folate and metabolites thereof at the same time. The kit comprises a calibration material, a quality control material, an internal standard liquid, a dissociation agent, a dissociation agent diluent, a mobile phase A liquid, a mobile phase B liquid, a reconstitution fluid, a 96-pore microplate, an operating instruction and a packaging box for separating and intensively packaging these reagent bottles or tubes. The content of main folic acid in a human body is quantitatively detected by using a liquid chromatography-tandem mass spectrometry with high specificity and accuracy, the current folic acid state is reflected and the clinic detection quality is improved; an operation error caused by a corresponding self-made reagent can be avoided; and meanwhile, the operation convenience and the result stability can be improved.

The invention discloses a flow-type detection reagent for detecting InhibinB content and a preparation method and application thereof. The flow-type detection reagent is characterized by comprising an Ab1 antibody microsphere conjugate and an Ab2 antibody fluorescent molecular marker which are used for detecting INH B content in serum or seminal plasma. The preparation method comprises: a step of preparing INHB alpha and beta B sub-unit antibodies; a step of coupling the INHB alpha sub-unit antibody Ab1 with mono-dispersion microspheres; a step of carrying out fluorescent molecular marking on the INHB beta B sub-unit antibody Ab2. By an antibody microsphere coupling technology and an antibody fluorescent marking technology and through adopting specific combination of antigen antibodies, microspheres and fluorescent molecules, which have a certain particle size, can be identified by a flow cytometry; fluorescent intensity directly reflects the INHB content in a sample; flow cytometry quantitative detection on the INHB in the sample is implemented; the flow-type detection reagent has the advantages of high sensitivity, high detection speed and high accuracy.

The invention discloses a large-scale preparation method and an application of a metal and metal derivative composited 2D carbon plate aerogel material and belongs to the technical field of preparation of functional nano-materials. Water is added to an agarose and metal salt mixture, a mixed solution is subjected to microwave heating treatment in a microwave reactor, composite foam of metal salt-agarose is obtained, the foam is further calcined, and carbon aerogel consisting of metal nanoparticle composited 2D carbon plates is obtained. The carbon aerogel prepared with the method has high specific surface area, high graphitization degree and relatively large 2D area, thereby having the characteristics of high conductivity, rapid ion transmission channels, highly active sites and the like,and having the advantages of high power capacity, high stability and high activity in energy storage and electrochemical catalysis reaction. The whole preparation process of the material is simple, the raw materials are cheap, and the material is suitable for large-scale production.

The invention relates to a method for measuring the practical solubility of calcium sulfate for papermaking filler. It belongs to the field of papermaking. Disperse an appropriate amount of absolute-dry calcium sulfate in water at a certain concentration, heat, stir, and filter the dispersion. From the quality difference between the filter residue and the absolute-dry calcium sulfate sample, calculate the temperature and concentration of calcium sulfate through the relevant formula. Practical solubility below. This experimental method solves the problem that the traditional calcium sulfate solubility determination method cannot objectively reflect and evaluate the loss of filler in the papermaking process and the retention rate of filler in filled paper.

The invention discloses a flow detection reagent for human anti-Mullerian hormone, a preparation method of the flow detection reagent and application. The reagent is characterized by comprising an antibody Ab1-microsphere conjugate used for detecting the content of anti-Mullerian hormone in human serum or blood, and an antibody Ab2-fluorescent molecular marker. The preparation method comprises the following steps: preparing and pairing human anti-Mullerian hormone monoclonal antibodies, coupling between the human anti-Mullerian hormone monoclonal antibodies and carrier microspheres, and fluorescent molecular marking of the monoclonal antibodies. The flow detection reagent has the advantages that the content of AMH is directly judged according to fluorescence values, an experimental result can be obtained only by adding a blood sample into the reagent, the experimental operation steps during detection are simplified, moreover, high-throughput and multi-sample continuous detection can be realized through an automatic sample adding system, the detection efficiency is greatly improved, and the detection sensitivity can reach 0.01ng/ml.

The invention provides a preparation method of micro-and nanoscale protein crystals capable of stably existing in air. Protein mini-crystals grow through the simple and feasible method, chemical cross-linking is conducted on the mini-crystals through a cross-linking agent, and the protein mini-crystals capable of stably existing in air are obtained. The method is convenient to operate, economical and practical; the prepared protein mini-crystals do not need crystal rings or capillary tubes when used for free electron laser diffraction, and background scattering can be eliminated; when the crystals are used as a catalyst, a sample can not be dissolved and can be repeatedly used.

The invention belongs to the technical field of genotyping detection method, and in particular, relates to a multi-PCR-SBT genotyping method for an ABO antigen of a human erythrocyte blood type system. The invention also relates to a multi-PCR-SBT reagent for ABO antigen genotyping of the human erythrocyte blood type system. The reagent and method provided by the invention can be used as an independent and widely used identification method, solve the problem of rapid and accurate typing of an ABO antigen system, and exert the characteristics of high-throughput operation and accurate results ofmulti-PCR-SBT on ABO genotyping. Relevant applications in the fields of clinical blood transfusion medicine research and genetics can be highly valued, and important practical significance are achieved in research departments, and pharmaceutical research and reagent development departments.

The invention discloses an experiment device for measuring direct-current breakdown voltage and pre-breakdown current of insulating liquid. The experiment device comprises a fixing seat including an upper insulating cover plate and a lower insulating cover plate, a high-voltage electrode assembly including a pin type electrode and a first conductive rod, a grounding electrode assembly including a plate-type electrode and a second conductive rod, and a test groove, wherein the upper insulating cover plate is provided with a first assembling through hole, the lower insulating cover plate is provided with a second assembling through hole, the first conductive rod comprises a first installing end and a first wiring end, the pin type electrode is arranged at the first installing end, the second conductive rod includes a second installing end and a second wiring end, the plate-type electrode is arranged at the second installing end, the plate-type electrode and the plate-type electrode are located the liquid level of the insulating liquid to be measured. Therefore, due to simple structure, light weight and small size of the experiment device, the experiment operating steps can be simplified, the cost can be reduced, meanwhile dismounting, cleaning and maintenance after test completion are promoted, and mounting and dismounting are convenient.

The invention discloses a poly-heterocyclic ring based acrylate monomer modified waterborne hydroxy acrylic dispersion and a preparation method thereof. The dispersion comprises, by weight, 20-50 parts of solvents, 5-10 parts of poly-heterocyclic ring based acrylate monomers, 50-70 parts of acrylic alkyl ester, 25-30 parts of acrylic functional monomers, 1-3 parts of chain transfer agents, 2-4 parts of initiators, 1-3 parts of anion exchange reactive emulsifiers, 2-4 parts of initiators, 100-120 parts of deionized water, 0.1-0.5 part of defoaming agents, 0.2-0.6 part of leveling agents and 0.3-0.8 part of wetting agents. The glossiness of a film is higher than 97 degrees, the hardness of the film reaches up to 2H, the film is strong in impact resistance, excellent in flexibility and adhesive force, and the dispersion has prominent chemical corrosion resistance such as acid resistance, alkali resistance and water resistance, meets natural zero-VOC (volatile organic compounds) paint standards, is environmentally friendly, non-toxic and free from pollution and can be used for waterborne wood paint, waterborne industrial paint and waterborne floor paint.

The invention relates to the molecular biology and biotechnology field, in particular to a high-throughput transcriptome library construction method. The method utilizes reverse transcription cDNA first strand synthesized Buffer as a fragmentation reaction system, so that fragmentation reaction and reverse transcription first strand synthesis can be linked up seamlessly, and the experimental operation steps can be simplified. During purification and recovery of the cDNA second strand synthesized and end repaired product in library construction, nucleic acid is utilized to purify magnetic beadsrepeatedly. During equivalent pooling of sequencing samples, precise pooling of samples can be achieved directly according to the Qubit detection result without absolute quantification of the samplesby qPCR. The above-mentioned improvements can effectively reduce the workload in the library construction process, and at the same time also greatly lowers the library construction cost.

The invention relates to a preparation method of electrodepositing high permeability iron-nickel alloy thin film on aluminum matrix composite material. The purpose of the invention is to solve the problem that the magnetic field shielding performance of the existing aluminum matrix composite material is very poor. The preparation method of the invention comprises the following steps: 1, removing the surface oil stain of the aluminum matrix composite material; 2, performing acid pickling light; 3, zinc dipping; 4, acid washing; 5, secondary zinc dipping; 6, electroplating an iron-nickel alloy;And 7, sealing. A Fe-Ni alloy film with high permeability is electrodeposited on an aluminum matrix composite material to form a structure of high magnetic conductivity, non-magnetic conductivity andhigh magnetic conductivity, and high magnetic shielding effect is realized through secondary shunting action. The invention is applied to the technical field of surface electrodeposition treatment.