20results about How to "Simplify the experimental operation process" patented technology

The invention discloses a construction method, a recognition method and a system for simulating lung cancer metastasis cells in cerebrospinal fluid based on diffraction imaging and a deep learning technology. The construction method comprises the following steps: culturing a plurality of lung cancer cell lines which are easy to cause brain metastasis, collecting whole blood of a healthy person and splitting red blood cells; the method comprises the following steps: respectively acquiring diffraction images of cultured cells through a polarization diffraction imaging flow cytometry with a TDI camera to obtain a pair of diffraction image pairs with S polarization and P polarization; performing image processing on the obtained diffraction cell image pair, and establishing a training image data set and a test image data set; and establishing a convolutional neural network, and training the convolutional neural network according to the training image data set to obtain a model for identifying the lung cancer metastatic cells in the cerebrospinal fluid. When in use, a cerebrospinal fluid sample of a patient is collected, S polarization and P polarization image pairs are obtained, and the trained model is input to respectively extract S polarization and P polarization image features of cells for feature recognition, so that whether the current sample contains lung cancer cells or not is predicted.

The invention provides an effective calcium content rapid self-detection method based on a conductivity test, and relates to the technical field of road engineering detection. The rapid self-detection method for the effective calcium content comprises the following steps: preparing an ammonium chloride solution and a lime stabilizing material; putting the lime stabilizing material into a beaker, and adding water to prepare a mixed solution; assembling and calibrating a conductivity meter, and erecting an electrode and a burette; carrying out a titration experiment by using an ammonium chloride solution, and detecting the conductivity of the solution by using a conductivity meter; recording the corresponding relation between the ammonium chloride solution titer and conductivity, drawing a curve image, and searching the ammonium chloride solution titer at a reaction end point; and calculating the effective calcium content in the lime stabilizing material according to the reaction endpoint titration amount. The effective calcium content in the lime stabilizing material is accurately detected through the conductivity change rule, the experiment steps are simplified, a standard curve does not need to be drawn, the detection time is shortened, and the detection and construction efficiency is improved.

The present application mainly relates to a kind of preparation of sulfur-rich amino acid protein CMd-copper nanoclusters and its detection mercury ion method, comprising: a) by prokaryotic expression obtains CMd protein, and the protein concentration of 5 mg / mL-25 mg / mL CMd protein solution is obtained by purification.b) CMd is reacted with reducing agent TCEP after opening protein disulfide bond.c) the protein solution after reduction is reacted with anhydrous copper sulfate, and preparation high fluorescence CMd-copper nanocluster.d) CMd-copper nanocluster is applied to detect heavy metal mercury ion.The application method can be synthesized under mild aqueous conditions by "one-step method" structure controllable, stable CMd-copper nanocluster, widens the raw material of copper nanocluster synthesized with protein as template, avoids the use of organic solvent and strong reducing agent, and reduces the cost and environmental burden brought by noble metal raw material, and also has higher selectivity to mercury ion.

The application relates to the fields of gene therapy and neuroscientific experimental technology, and particularly relates to a mobile injection device for non-human primate brain area AAV injection, which comprises a ring, a mounting frame rotatably connected to the top of the ring, a first driving structure installed between the ring and the mounting frame and used for driving the mounting frame to rotate on the ring, a sliding plate slidably connected to one side of the mounting frame and located in the interior of the ring, a second driving structure installed on the mounting frame and used for driving the sliding plate to move up and down, a swing plate assembled at the end of the sliding plate away from the mounting frame, an injection needle installed at the top of the swing plate, a pushing structure installed at the top of the swing plate, and an assembling structure also installed at the top of the swing plate. The injection device is provided with a structure for synchronous injection during the needle withdrawal process, can complete virus delivery of the target brain area through a single needle channel, reduces the number of puncture needle channels, and reduces mechanical damage to the brain tissue.

The invention discloses a high-flux toxicity pre-evaluation substitution method based on an acetylcholin esterase target. By integrating an SH-SY5Y nerve cell endogenous AChE and recombinant exogenous AChE dual detection system and relying on an automatic screening system constructed by integrating core equipment such as an automatic pipetting workstation, a constant-temperature incubator and a multifunctional microplate reader, a high-flux toxicity pre-evaluation substitution method based on an AChE target is successfully established and is used for evaluating the neurotoxicity of a compound, and the method has the advantages of high sensitivity, high sensitivity, high sensitivity and the like. The method solves the limitation that a single screening method in the prior art cannot evaluate factors such as target inhibition, cell permeability and metabolic transformation at the same time, and the method is simple, convenient, rapid, high in accuracy and suitable for large-scale primary screening of the AChE inhibitor and early warning of neurotoxicity risks.

This invention discloses a computer program product and its application for data processing devices in the field of bioinformatics for ctDNA variant detection. The technical problem this invention aims to solve is how to detect ctDNA variants in early-stage cancer or postoperative minimal residual disease (MRD) under conditions of no UMI library construction and moderate sequencing depth. This invention constructs a set of supporting sequences for candidate variant sites, generating a sequence feature tensor and a fragment physical feature vector (including normalized fragment length). The former is input into a first neural network branch to extract sequence representation, and the latter into a second neural network branch to extract physical representation. A fusion module combines the sequence representation and physical representation, and a gating unit calculates the gating weight based on the physical representation and dynamically adjusts the contribution of the sequence representation, outputting the probability of the true ctDNA variant. This invention utilizes physical laws to suppress sequencing noise and can be applied to monitor MRD under conditions without molecular barcodes.

The utility model relates to the technical field of physical experiment teaching aids, in particular to a simple harmonic motion experiment instrument, which comprises a base, a liquid spraying device, a support arranged on the base and a paper feeding device arranged on the base, the support comprises a cross rod, a first vertical rod and a second vertical rod, the first vertical rod and the second vertical rod are arranged on two opposite sides of the cross rod, and the cross rod is provided with a cycloid and connected with a pendulum ball. A gap exists between the lowest point of the swing ball in the natural state and the base, a spring is fixed to the first vertical rod and is horizontally placed in the natural state, one end of the spring is detachably connected with the swing ball, the liquid spraying device comprises a spraying pipe, and the liquid outlet end of the spraying pipe is fixedly connected with the swing ball; a spring oscillator experiment and a simple pendulum experiment are integrated on one device, so that students can conveniently compare two simple harmonic motion phenomena on the same platform, the essence and generality of the simple harmonic motion can be deeply understood, and the teaching effect is optimized. Meanwhile, through the detachable connection design, quick switching between a spring oscillator experiment and a simple pendulum experiment is facilitated, and different experiment requirements are met.

The application relates to the technical field of magnetism measuring instruments, in particular to a magnetic sub-space imaging scanning device and method based on the magneto-optical Kerr effect. The device comprises a transmitting assembly, a scanning assembly and an imaging assembly. The transmitting assembly comprises a laser, a beam expander, a beam splitter, a mirror, a first half-wave plate, a polarization beam splitting cube, a calcite polarizer, a first dichroic mirror, an objective lens, an LED light emitting component, a convex lens and an achromatic doublet lens, so as to focus an incident light beam on a to-be-measured object. The scanning assembly comprises a second dichroic mirror, a second half-wave plate, a mirror, a beam splitting prism, a right-angle reflecting prism, an achromatic doublet lens, a photoelectric balance detector, a lock-in amplifier, a magnetic field applying component and a piezoelectric displacement stage, so as to scan the to-be-measured object. The imaging assembly comprises a convex lens, a CMOS camera and a data processing component, so as to generate a topographic image and a spin wave image. The application can perform high spatial resolution, high magnetic sensitivity and wide frequency band spatial imaging measurement on spin waves and magnetic sub-frequency combs.

The invention provides a construction material impermeability detection device and a detection method thereof, and relates to the technical field of concrete impermeability detection.The construction material impermeability detection device comprises a box body, a pressure water supply system and a plurality of detection modules, and each detection module comprises an annular bottom plate, a guide frame, an annular bracket, an annular frame, a push strip and a Z-shaped clamping piece. When the spray head assembly is rotated to be in a first position range, the inner convex plate and the outer convex plate coincide, the annular bracket can drive the spray head assembly to extrude the bottom end of the concrete detection block, a cavity for supplying pressure water is formed, and sample detection can be rapidly completed; when the rotary spray head assembly is located in a second position range, the inner convex plate and the outer convex plate are staggered, the spray head assembly is not limited by vertical movement of the annular bracket, loading and dismounting of the concrete detection block can be conveniently completed by means of the annular bracket, and compared with a traditional anti-permeability detection device, experiment operation steps are simplified, the labor intensity of personnel is reduced, and the working efficiency is improved. And the detection efficiency is improved.

The utility model provides a nonlinear element characteristic measurement experiment instrument. The nonlinear element characteristic measurement experiment instrument comprises an experiment instrument panel, a state display module, a nonlinear element to-be-measured area, an AC / DC conversion power panel and a voltage measurement module are arranged on the experiment instrument panel; the AC-DC conversion power supply board at least comprises a power supply module and a circuit building module, and the power supply module is connected with the nonlinear test element and is also connected with the circuit building module, the voltage measurement module and the external oscilloscope in sequence; the circuit building module comprises a first connection area, a second connection area and a third connection area; the voltage measurement module comprises an A / D conversion chip and a voltage measurement calibration circuit. The voltage measurement calibration circuit is composed of a reference voltage source, an adjustable potentiometer RV1 and a voltage division network with a preset voltage division ratio. The nonlinear element characteristic measurement experiment instrument is simple in structure, convenient to operate and accurate in measurement, the teaching quality of experiments is improved, and the failure rate and the maintenance time in the experiments are reduced at the same time.

The present invention discloses a method and device for preparing metal materials by multi-magnetic field assisted directional solidification, including a smelting device, a magnetic field generating device, a vacuum cavity, a cooling tank, a temperature measuring system, a pulling device, a vacuum system and an experimental atmosphere source; the cooling tank is arranged at the bottom of the vacuum cavity, the smelting device is arranged in the vacuum cavity, the magnetic field generating device is arranged in the cooling tank, the vacuum system is connected to the inner cavity of the vacuum cavity, and is used to evacuate the vacuum cavity, the experimental atmosphere source is connected to the inner cavity of the vacuum cavity, and is used to fill the experimental atmosphere into the vacuum cavity after evacuation; the temperature measuring system is used to monitor the upper surface temperature of the sample in the vacuum cavity; the present invention regulates the pulling speed, magnetic field strength and magnetic field type of directional solidification during the directional solidification of the metal melt, and can design the material structure according to the requirements of different performances of different sections of the material in complex application environments. The prepared material has gradient organization and performance along the radial direction.

The invention provides a continuous flow synthesis method of zidovudine, which comprises the following steps: sequentially carrying out continuous hydroxyl protection (S1), continuous acylation (S2), continuous cyclization (S3), continuous azidation (S4) and continuous deprotection (S5) on a raw material compound beta-thymidine to obtain a zidovudine crude product. According to the technical scheme, a more efficient, safer and more flexible production mode and method are provided, hydroxyl protection, acylation, cyclization, azidation and deprotection reactions are continuously operated, the reaction efficiency and selectivity are improved, a large amount of separation and purification operation in the original production process is reduced, and the production cost is reduced. And the production time can be shortened from several weeks to several hours.

The invention relates to the technical field of analytical chemistry. The invention provides a multi-component identification method based on catalytic luminescence and an artificial neural network, and the method comprises the steps: carrying out the catalytic luminescence reaction through a single sensing unit and a plurality of training samples, collecting a corresponding catalytic luminescence response signal, and obtaining an initial data set; preprocessing the initial data set to obtain a preprocessed data set; constructing a component identification initial model based on a neural network, and training the component identification initial model through the preprocessing data set to obtain a component identification model; and collecting a catalytic luminescence response signal of a to-be-detected sample, performing pretreatment, and inputting the pretreated catalytic luminescence response signal of the to-be-detected sample into the component identification model to obtain a component identification result of the to-be-detected sample. The problems that most of existing catalytic luminescence technologies are limited to single-component detection, a multi-component system needs to be detected by a plurality of sensing units respectively, the efficiency is low, and simultaneous recognition of multiple components is difficult to achieve are solved.

The application relates to an organ chip clamp and an organ chip device. The organ chip clamp comprises a base and a cover plate. The base is provided with a placement area for placing a chip body, and the placement area is provided with a first pneumatic interface for communicating with a first gas inlet and outlet of the chip body; the first pneumatic interface is used for being connected to a gas pressure vacuum controller. The cover plate is connected with the base and is used for abutting to one side of the chip body away from the base, the cover plate is provided with a first sealing piece for sealing a liquid inlet and outlet of the chip body, and the cover plate is also provided with a second pneumatic interface for communicating with a second gas inlet and outlet of the chip body. The second pneumatic interface is used for being connected to the gas pressure vacuum controller. The organ chip clamp and the organ chip device can reduce experimental result error.

The invention provides a live grape leaf instantaneous transformation method, and belongs to the technical field of breeding. The method comprises the following steps: (1) culturing grape seedlings; (2) instantaneous transformation of grape leaves: taking hydroponic grape seedlings in a suitable transformation stage, and wrapping grape root systems with wet cotton; completely intruding grape leaves into the infection penetrating fluid, and vacuumizing; taking out the grape seedlings after preset time, continuously placing the grape seedlings in the Hoagland culture solution, and recovering culture. According to the method, the root system of the grape is wrapped by the wet cotton, so that the root system is prevented from being damaged by vacuum water loss, the condition that the leaves are mechanically damaged due to transformation in a traditional syringe or centrifugal tube is avoided, and the grape leaves are completely intruded into the infection penetrating fluid, so that the uniformity and stability of transformation of the living leaves can be ensured; according to the method, the transformation efficiency and stability are effectively improved, the transformation period can be remarkably shortened, more efficient and reliable technical support is provided for grape gene function research, and the method has wide application prospects.

The invention relates to an industrial preparation method and application of alpha-hydroxynitrile. The invention belongs to the technical field of fine chemical synthesis. The invention aims to solve the technical problems of low yield, high cost and unsuitability for industrial production of the existing method for synthesizing alpha-hydroxynitrile. According to the method, the alpha-hydroxynitrile is synthesized through the reaction of aldehyde ketone carbonylate and acetone cyanohydrin under the action of the ionic liquid. According to the method disclosed by the invention, the green and high-yield synthesis of the alpha-hydroxynitrile is realized by selecting the ionic liquid and synergistically and reasonably controlling the ratio of the substances, and the possibility is provided for reducing the industrial production cost of the alpha-hydroxynitrile. The method is simple in experimental operation process, and can be widely applied to synthesis in the fields such as medicines in industrial and academic circles.

The invention discloses a vegetation concrete anti-scouring intelligent test device and a use method, and relates to the field of vegetation concrete anti-scouring simulation tests. The device comprises a base frame, a rainfall simulation device, a steering slope, a muddy water collecting box and a muddy water drying and weighing integrated device, the muddy water drying and weighing integrated device is arranged on the base frame, the muddy water collecting box is arranged on the muddy water drying and weighing integrated device, the steering slope is arranged on the muddy water collecting box, and the muddy water drying and weighing integrated device is arranged on the steering slope. And a rainfall simulation device is arranged above the steering slope surface and is connected with the base frame. According to the vegetation concrete anti-scouring intelligent test device adopting the structure and the use method, the limitation of a traditional test is overcome, the operation is simple and convenient, the measurement is convenient, the simulation steps are greatly simplified, and materials and manpower are saved.

The invention discloses a temperature control stirring photosensitive timing device which comprises an experiment support, a control box and a magnetic stirrer, the experiment support is detachably installed on the magnetic stirrer, the experiment support is a disc-shaped hollow support, the experiment support is provided with a reaction cup placing position and a solution cup placing position, and the reaction cup placing position and the solution cup placing position are arranged on the control box. A first mounting part and a second mounting part are arranged on the side wall of the reaction cup placing position, a light supplementing lamp is mounted in the first mounting part, and a photosensitive sensor is mounted in the second mounting part; the control box is fixedly installed on the experiment support, a control circuit board is installed in the control box, the light supplementing lamp and the photosensitive sensor are connected with the control circuit board, and a timer, an indicating lamp, a photosensitive threshold adjusting button and a reset button are installed on the surface of the control box. According to the invention, the color change of the reaction liquid can be automatically monitored, timing is carried out, the problem of experimental errors caused by misjudgment of experimenters is solved, the accuracy of the chemical reaction rate experiment is improved, and the experimental operation steps are simplified.

The invention discloses a method and system for evaluating microscopic damage of a rock core based on low-field nuclear magnetic resonance, and belongs to the technical field of exploration. Nuclear magnetic resonance detection is performed on a soaked rock core by using a CPMG sequence, and a spin echo string attenuation signal is recorded to obtain attenuation distribution of T2; fitting inversion is carried out to obtain a porosity component and an inversion curve of attenuation distribution of T2 in the inversion process; determining the fluid content in different apertures according to the position of the T2 inversion spectrum peak; establishing a linear relation between the accumulated porosity component of the T2 inversion spectrum change spectrum peak and the soaking time; and quantitative characterization can be carried out on the microscopic damage of the rock core according to the slope in the linear relation. According to the method, the problem that the damage condition of the drilling fluid to the rock core cannot be accurately and quantitatively represented in the prior art can be solved, and the conditions of the rock core in different solutions are inspected, so that the damage degree of the drilling fluid to the rock core can be evaluated.

The invention belongs to the field of photoelectrochemical detection, and discloses a photo-assisted zinc-air battery space-time coordination dual-mode self-energized sensor for gallic acid detection and a preparation method of the photo-assisted zinc-air battery space-time coordination dual-mode self-energized sensor. The photocathode designed by the invention comprises a visual area and an electron injection area which are connected with each other through an electron transfer channel. Wherein the visual area is formed by modifying Prussian blue on the FTO conducting layer; the electron injection region is formed by sequentially modifying BiFeO3, AgBr and a molecularly imprinted polymer (MIP) on the conducting layer and is marked as MIP / AgBr / BiFeO3 / FTO; the electron transfer channel is an unmodified conducting layer. The zinc-air battery self-energized electrochemical sensor is constructed by taking eluted MIP / AgBr / BiFeO3 / FTO as a photocathode and a zinc plate as an anode, and is used for quantitative analysis of gallic acid (GA), and the self-energized electrochemical sensor is combined with an electrochromic technology, so that background interference is effectively eliminated, dual-mode sensing can be realized at the same time and in the same space, and the application prospect is wide. And the experiment operation process is simplified.