52 results about "Primate" patented technology

The invention belongs to the technical field of computer vision and image recognition, and particularly relates to a fine-grained image block embedded multi-task emotion recognition method, which comprises the following steps of: constructing a golden snub monkey multi-modal emotion data set for wild primate animals, and covering emotion, individual and gender multi-dimensional labels; the method comprises the following steps: preprocessing an input wild primate image, dividing the input wild primate image into non-overlapping local image blocks with fixed sizes through blocking and feature extraction, and mapping the non-overlapping local image blocks to a high-dimensional feature space through linear projection to form a series of image block embedding vectors; performing local feature modeling on the image block embedded vector based on a fine-grained local scanning module to enhance fine-grained perception of local features such as facial expression and hair texture of the golden snub monkey, and performing global feature modeling on the image block feature vector by a global scanning module to enhance global semantic representation; according to the invention, the performance and generalization ability of multi-task identification of golden snub monkeys are improved.

The invention discloses a multi-modal imaging system and method for a small animal living body. The system comprises a multi-modal fusion imaging module which is responsible for multi-modal image acquisition and primary processing; the real-time dynamic monitoring module is used for capturing bioluminescence / fluorescence signals in real time through a photon counting detector, receiving optical, nuclear medicine and magnetic resonance data, correcting motion artifacts based on a multi-scale space-time registration engine and adjusting scanning parameters through a real-time pharmacokinetic-physiological feedback mechanism; the low-radiation and biological compatible module is used for automatically optimizing the dosage of a tracer agent according to the weight of the animal, the scanning part and historical data by using a dosage prediction model; and the multi-modal data fusion module is used for performing non-rigid registration of multi-modal images based on a Transform cross-modal registration network, constructing a multi-species pharmacokinetic knowledge graph by utilizing multi-species metabolism chip data, integrating mouse, dog, primate and humanized liver chip data, and mining a cross-species metabolism rule through a graph neural network.

The construction of a non-human primate Usher syndrome disease model having USH2A gene mutation, wherein a target sequence against the mRNA of the non-human primate USH2A gene is established and a corresponding sgRNA is synthesized, then a knockout vector is constructed, the sgRNA and a Cas9 mRNA are mixed and injected into a non-human primate embryo to obtain a non-human primate embryo having USH2A gene knockout, and then by means of embryo transfer, a non-human primate having USH2A gene knockout is obtained, which exhibits symptoms of Usher syndrome. Upon detection and identification of the knockout effect, the knockout of the USH2A gene can be effectively realized in a non-human primate cynomolgus monkey embryo and cynomolgus monkeys, which provides a new and effective disease model for the diagnosis and treatment of Usher syndrome in non-human primates.

The invention belongs to the field of biological medicine research and development, discloses a method and a system for collecting and analyzing auditory electrophysiological data of experimental animals, and is particularly suitable for non-human primates. The method comprises the following steps: delivering a drug to a middle ear cavity through an external auditory canal, and synchronously recording auditory electrophysiological data before and after drug administration; aBR and DPOAE multi-mode detection is adopted, and the frequency range of 4 kHz to 32 kHz is covered; setting anesthesia depth and three-electrode positioning to reduce interference; optimizing the signal-to-noise ratio through noise suppression and dynamic signal superposition; an auditory threshold database is constructed, and a machine learning model is combined to judge an injured part. The system integrates a multi-modal stimulation module, a minimally invasive drug delivery device and a data analysis platform, and supports cross-species data compatibility. The accuracy of otology medicine research is remarkably improved, the surgical operation risk is avoided, and the method is suitable for gene therapy, ototoxicity screening and other scenes.

The utility model relates to the technical field of animal ability detection, and particularly discloses a non-human primate cognitive ability detection device with double functions, which comprises an experiment table, an animal box and a reward box, the animal box is used for animals to enter; the front end face of the animal box is provided with a through opening allowing the hand of an animal to stretch out. A movable baffle is arranged on the front end surface of the animal box; the baffle is used for controlling opening and closing of the port; the experiment table is arranged at the front end of the animal box; rewards for attracting animals to pick up are placed in the reward box; the award box is provided with an opening into which animals can extend; the reward box is movably arranged on the experiment table, and the direction of the opening of the reward box can be switched. According to the detection device provided by the scheme, the award box can move on the experiment table to change the distance between the award box and the animal box, and the opening orientation of the award box can be changed to adjust the experiment difficulty, so that compared with an existing experiment device, the diversity of experiments can be effectively improved, and then the effectiveness of animal cognitive ability detection is improved.

The present disclosure relates to compositions and methods of treating stroke, reducing neuroinflammation, and generating new neurons in a primate using an adeno-associated viral (AAV) vector encoding NeuroD1.

To provide compositions and methods for treating Leber congenital amaurosis (LCA) in a subject.SOLUTION: In one aspect, a recombinant adeno-associated viral vector is provided which includes a nucleic acid molecule comprising a sequence encoding Lebercilin. In another aspect, Lebercilin has a specific amino acid sequence. In yet another aspect, the nucleic acid molecule has a specific sequence different from the above sequence, or a variant thereof. In desired embodiments, the subject is human, cat, dog, sheep, or non-human primate.SELECTED DRAWING: Figure 1-1

The invention relates to a touch fluid neuron specific tracing imaging agent, a preparation method and application, and belongs to the technical field of nuclear medicine imaging, the tracing imaging agent is composed of a nerve tracer agent horseradish peroxidase (HRP), zirconium-89 isotope chelated by deferoxamine and a pharmaceutically acceptable carrier, the effective tracking window of the tracer agent is prolonged to 120 hours, and the effective tracking window of the tracer agent is prolonged to 120 hours. The multi-stage synaptic transfer period of the fluid touching neuron can be completely covered; the spatial resolution is improved by 5-8 times compared with that of traditional FDG-PET, and a fluid touching neuron cluster can be accurately presented; the in-vitro serum stability is excellent, the radiochemical purity is still larger than or equal to 80.5% within 120 hours, PET / MRI bimodal imaging can be adapted, drug administration is conducted through lateral ventricle minimally invasive injection, the probe is suitable for various animal models such as mice, rats and non-human primates, in-vivo dynamic tracing of sympathetic fluid neuron trans-synapses can be achieved on the premise that no tissue damage exists, and the probe has a good application prospect. And a key technical tool is provided for neural circuit analysis, neuroscience basic research and preclinical research of neurodegenerative diseases.

The invention relates to a barcoding method to assess transduction efficiency of AAV vectors, in particular in vivo in rodent and non-human primate.

The present invention belongs to the technical field of cell biology, and particularly relates to the application of Hippo-Yap pathway regulators in promoting the directed differentiation of induced pluripotent stem cells into pancreatic progenitor cells. The method of the present invention can efficiently obtain pancreatic progenitor cells derived from non-human primate rhesus monkeys in vitro, providing a material source for further obtaining functional pancreatic islet β cells of non-human primate rhesus monkeys, and more importantly, providing a practical basis for the autologous transplantation treatment of non-human primate diabetic animal models.

The invention discloses a sperm genome methylation detection method for safety evaluation of biological breeding crops by using primates and application, and relates to the technical field of safety evaluation of crops, the sperm genome methylation detection method comprises the following steps: dividing non-human primates into three groups, collecting sperms after long-term feeding, and extracting DNA (Deoxyribose Nucleic Acid); carrying out whole genome sequencing and quality control after bisulfite treatment; the epigenetic safety of crops is comprehensively evaluated by analyzing the methylation level of a whole genome and a functional region and functional enrichment of a differential methylation region and related genes thereof; according to the sperm genome methylation detection method for evaluating the safety of the biologically bred crops by utilizing the primates and the application, by utilizing a high-resolution WGBS technology, subtle epigenetic changes which are difficult to find by traditional toxicology can be detected, and the sperm genome methylation detection method has important significance in cross-generation reproduction effect evaluation, and has a wide application prospect. A food safety evaluation system can be perfected, and a more scientific and reliable safety interpretation basis can be established.

This application relates to a modular non-human primate rearing cage, belonging to the technical field of animal husbandry. It includes a cage frame, a resource supply system, a hygiene and cleaning system, an activity and enrichment system, and a connection and management system. The cage frame includes a cage base, a cage top, and cage side frames. The resource supply system includes a food supply device and a water supply device detachably installed on the cage frame. The connection and management system includes a connecting channel on the cage side frames and a connecting cage door movably installed on the cage side frames for changing the opening and closing state of the connecting channel. The activity and enrichment system includes a lookout component installed on the cage side frames, a climbing component located between the lookout component and the cage base, and multiple monitoring units installed on the cage top frame. This application can improve the husbandry and management efficiency of non-human primates.

The present invention provides a flexible sensor for deep brain detection in humans and non-human primates, comprising a flexible electrode and a flexible printed circuit board (flexible PCB); wherein the flexible electrode includes an implantation end and a pad area, the length of the implantation end is 50–70 millimeters, detection sites are distributed thereon, pads are distributed in the pad area, the detection sites are used for detecting nerve signals, the pads are used for welding with the flexible PCB, and the detection sites and the pads are connected by wires; the hierarchical structure of the flexible electrode includes a base layer, a conductive layer and an insulating layer, wherein the base layer and the insulating layer are parylene flexible films, and the conductive layer is composed of a chromium layer and a gold layer. The present invention also discloses a welding method for the flexible sensor, which uses a method of spot hot melting of solder to weld the flexible electrode and the flexible PCB. This welding method can achieve precise alignment and tight connection of the solder joints, the conduction rate of the sensor is good, and it can avoid the rupture and deformation of the flexible sensor. The flexible sensor prepared by the present invention has strong flexibility, high transparency, and small implantation damage, and can detect the brain nerve signals of humans and non-human primates for a long time.

This disclosure relates to the fields of computer vision and wildlife monitoring technology, and specifically to a method and system for identifying Tibetan macaques in the wild based on deep learning. First, a face detection model is used to detect and crop faces from raw wild images. The detection model is initialized with pre-trained weights and fine-tuned by freezing the backbone network. Then, an individual identification model is constructed to output classification results. During training, a joint loss function is used, combining Focal Loss to suppress easily classified samples and guide attention to difficult-to-classify samples. Supervised contrastive loss is used to enhance the discriminative power of the feature space. Simultaneously, an adaptive hybrid enhancement strategy and a hierarchical Dropout regularization mechanism are introduced to effectively address the problems of small sample sizes, class imbalance, and overfitting in complex wild environments. This disclosure enables high-precision identification of Tibetan macaques, providing an efficient technical means for long-term ecological monitoring of wild primates.

Capsid protein mutants capable of increasing the ability of AAVs to infect ocular tissue and the use thereof. A Cap mutant is obtained by replacing an amino acid sequence of an AAV capsid protein which is homologous to amino acids at positions D561 to R588 of a wild-type AAV2 capsid protein with one of the polypeptides as shown in SEQ ID NOs. 6-39. Some Cap mutants can infect a mouse retina from the RGC layer, cone and rod cells of INL and ONL, corneal limbus regions, trabecular meshwork, RPE and a small amount of RPE cells by administration via intravitreous injection, can solve the problem that it is difficult for the conventional serotype AAV to infect the cells in the posterior layer of the retina due to insufficient penetrability, and is expected to become a potential carrier for the treatment of glaucoma. Experimental data show that the abilities of some Cap mutants to infect and penetrate the retina of non-human primates are optimized, thereby providing a new tool for developing gene therapy preparations for ophthalmic diseases or constructing animal models related to ophthalmic diseases.

The invention provides a targeting reagent for preparing fragile X syndrome animals and application of the targeting reagent. The invention discloses a target which is matched with non-human primates and is suitable for gene modification, namely a third exon and / or a fourth exon of an FMR1 gene, a target reagent is prepared by targeting the target, and a fragile X syndrome animal model with typical disease symptoms can be obtained by implementing down-regulation.

This invention discloses a neural microprobe for bimodal functional localization and precise electrical stimulation of deep brain nuclei in non-human primates. It comprises a basal layer, a conductive metal layer, and an insulating layer. The conductive layer includes a set of detection sites and stimulation sites. This invention employs a dry etching process on the back of the silicon basal layer using two insulating layers with opposite stresses, balancing the stress differences between the different materials. This overcomes the technological challenges of extending silicon microprobes and solves the problems of probe bending and low mechanical stress caused by the large aspect ratio of silicon probes in non-human primates. The detection sites acquire bimodal signals using modified nanomaterials and polymer anti-interference ion-exchange membranes as localization markers for functional localization. The detection sites are arranged between the positive and negative electrodes of the electrical stimulation, controlling the stimulation range to be near the detection sites, thus achieving precise electrical stimulation. This invention provides a new research method and means for deep brain stimulation therapy of diseases such as Parkinson's disease.

The application discloses a flexible neural electrode with a multilayer bidirectional signal transmission structure and a preparation method thereof. The neural electrode comprises a substrate insulating layer, at least two signal transmission layers, an intermediate insulating layer and a packaging insulating layer. The intermediate insulating layer is located between the signal transmission layers. The signal transmission layers are stacked in a sandwich structure of an adhesive / seed layer-metal-conductive layer-metal-adhesive / seed layer-metal. The intermediate insulating layer and the packaging insulating layer are windowed at the insulating layer of the orthographic projection of signal detection sites and pads of all layers below. The application further discloses a preparation method of the flexible neural electrode with the multilayer signal transmission structure. The neural electrode adopts a 3D stacking structure of the signal transmission layers, reduces the implantation width of the electrode, increases the number of detection sites, reduces the damage to the implanted brain tissue, improves the detection flux, and is helpful to realize long-term collection of high-density and high-resolution neural signals of brains of rodents, primates and the like.

The present application relates to the technical field of artificial intelligence, in particular to a method and system for identifying apes based on metric learning and a medium, the method comprising: obtaining original images of apes, performing data enhancement on the original images to obtain training samples; constructing an SE-ResNet model, inputting the training samples into the SE-ResNet model for training to obtain an ape identification model; wherein the SE-ResNet model comprises a plurality of residual modules, each residual module comprises a plurality of residual blocks embedded with SE modules, and the SE modules are used for adaptively adjusting the weights of each feature channel; the ape identification model is used for feature extraction and classification of ape images, and the individual and population categories of apes are identified simultaneously; the present application can efficiently identify the individual and population of apes.

The invention relates to the field of medicines, in particular to a construction method of an Alzheimer's disease non-human primate model. A small dose of thiamine antagonist pyrithiamine is given to the chronic abdominal cavity to induce chronic thiamine metabolic disorder, so that the non-human primate marmoset Alzheimer disease model capable of reflecting the Alzheimer disease course is established, and the method has the advantages of simplicity in operation, small animal injury and the like; therefore, the method is beneficial to further basic clinical transformation research and the like, and has more important scientific significance and economic value.

The present application relates to the field of animal experiment equipment, and specifically discloses a primate hand action testing machine, which comprises a box body, a first channel, a second channel, a first opening and a second opening. A storage assembly composed of a base and guide rods of different structures is placed in the first channel. A liftable movable tray is arranged in the second channel. An activity evaluation device capable of being used in cooperation with the hand action testing machine is also disclosed, which comprises a cage body, a seat body, a climbing rod, a first flexible column and a second flexible column. Food is placed on the first channel, the second channel and the guide rods to test the simple grabbing and fine finger movement of the animal, thereby achieving the effect of testing the hand action. The first flexible column flexibly connects the cage body and the seat body, and the second flexible column flexibly connects the climbing rod and the front and rear grilles. The pressure change of the pressure point of the pressure sensor can be used to evaluate the activity of the animal in the cage.

The vacuum barrel suitable for the outside of the primate comprises a negative pressure cup and a handle, one end of the negative pressure cup is provided with a sealable flexible inlet used for being inserted into an external object and forming sealing, so that negative pressure generated in the cup acts on the external object, and the handle is movably connected to a hard shell of the negative pressure cup. And a user can conveniently and flexibly control the negative pressure cup. Operating keys of a vacuum system of the vacuum barrel can be arranged on the front side face of a hard cup base of the vacuum barrel or the outer side face of a holding part of a handle, and operation by a user is facilitated. By optimizing the connecting structure of the negative pressure cup and the handle, various handle mounting schemes are provided, the use convenience, operation stability and portability of the device are remarkably improved, and the device has the advantages of being compact in design structure, easy and convenient to operate and good in user experience.

This utility model belongs to the field of animal disease prevention, specifically an animal blood collection device, including a tray. A support plate is fixedly connected to the bottom of the tray near the rear side. A locking block is engaged on the outer side of the support plate. A wall is fixedly connected to one side of the locking block, and a window is opened on the surface of the wall. After aligning the locking block with the support plate, a pin passes through the inside of the sleeve and the mounting ring, while a positioning post passes through the inside of the pin, so that the tray is vertically mounted on the front side of the window, which facilitates disassembly and assembly and fixation of the device in front of the window, improving the adaptability and stability of the device. Secondly, by enticing the forelimbs of primates to be placed on the surface of the tray, the closing limiting ring is rotated and the tension spring on the side of the tray pulls the buckle. The buckle rotates on the front side of the tray, so that the buckle engages with the surface of the locking post. The test tube to be used is placed through three fixing clips on the side of the tray, and blood is collected through a needle, improving the user's work efficiency and safety.

Disclosed are an antibody targeting the monkeypox virus, an antigen-binding fragment thereof, and a use thereof. The antibody or the antigen-binding fragment thereof comprises a heavy chain variable region and a light chain variable region. The antibody or the antigen-binding fragment thereof of the present invention targets A35R of humans and non-human primates.

The utility model provides a mobile non-human primate weighing device which comprises a bottom frame, a base, a support and an operation platform, the base is detachably arranged on the bottom frame, the support is arranged on the base, the operation platform is arranged on the upper portion of the support, and the base can be placed on a platform scale after being detached; the support comprises a connecting square pipe, at least one set of grabbing rods and a mounting support, the bottom of the connecting square pipe is connected with the base, the grabbing rods are arranged in the middle of the connecting square pipe, and the mounting support is arranged on the top of the connecting square pipe. Due to the design of the grabbing rod, the grabbing response under the conditions that non-human primates like to climb and are tense is met, and the operation process is more stable or rapid adaptation is achieved in the weighing process. The movable animal cage is simple in structure, reasonable in design, easy to fix animals and high in moving performance due to the fact that the open type frame structure is adopted, and meanwhile the anti-collision corners are installed at the four corners so that used facilities can be protected.

The invention discloses a construction method and application of a dog depression model. According to the method, the problems of dog allergy and fever in the modeling process are solved by injecting corticosterone through stepped increasing administration, and the dog depression model is successfully constructed for the first time. A large number of experiments prove that the dog depression model established by the invention successfully simulates the core phenotypes of human depression, such as pleasant sensation deficiency, low emotion, reduced interest and the like, and the major depression phenotype self-disability behavior appears for the first time. The method is simple, the molding period is greatly shortened, the success rate is increased to 80%, drug screening and pharmacology and toxicology synchronous research are supported, and the drug research and development cost is reduced. According to the invention, the problems of low clinical depression correlation and long non-human primate large animal modeling period of the existing mouse model are solved, and a standardized large animal platform with high prediction value is provided for research and development of antidepressant drugs.

The invention belongs to the technical field of regenerative medicine, and discloses an organoid culture method derived from neural stem cells in subventricular regions of human and non-human primates. By optimizing a culture medium formula and regulating and controlling transcription factors, the organoid is successfully induced to be differentiated into the neuron precursor for expressing the CRABP1. The cell expresses TAC3, the limitation of the prior art in simulation of special neuropedigree and complex brain structure of primates is overcome, and a breakthrough in-vitro model is provided for research on neurodevelopment and nervous system diseases and drug development. The established system not only can provide a new tool for researching the development law and disease mechanism of the primate nervous system, but also can provide a reliable experimental platform and a transplantable cell source for developing targeted drugs, constructing advanced disease models and developing cell replacement therapy.

The application discloses a non-human primate cage door locking device, which comprises a cage door, a T-shaped bolt lock and a locking structure. The cage door is provided with a plurality of cylindrical sleeves. The T-shaped bolt lock comprises a lock core part and a handle part. The lock core part is a horizontal circular rod in the axial direction, and the handle part is a vertical circular rod in the axial direction. The top end of the handle part is welded at the midpoint of the lock core part. The two ends of the lock core part are respectively inserted into the cylindrical sleeves. The T-shaped bolt lock can move left and right relative to the cage door. A through hole is formed in the T-shaped bolt lock. One end of the locking structure penetrates through the through hole and is detachably mounted on the T-shaped bolt lock. The non-human primate cage door locking device has the advantages of simple structure, easy operation, low cost, easy manufacturing, difficult learning of the opening and closing mode by monkeys, prevention of the monkeys from opening and closing the cage door by themselves, great improvement of the working efficiency of non-human primate cage production personnel and solution to the difficult problems in the intensive feeding of non-human primates.

The invention relates to the field of medicines, in particular to a construction method of an Alzheimer's disease non-human primate model. A small dose of thiamine antagonist pyrithiamine is given to the chronic abdominal cavity to induce chronic thiamine metabolic disorder, so that the non-human primate marmoset Alzheimer disease model capable of reflecting the Alzheimer disease course is established, and the method has the advantages of simplicity in operation, small animal injury and the like; therefore, the method is beneficial to further basic clinical transformation research and the like, and has more important scientific significance and economic value.