45 results about "Mycoplasma genitalium" patented technology

The present invention relates to oligonucleotides useful for determining the presence of Mycoplasma pneumoniae and/or Mycoplasma genitalium in a test sample. The oligonucleotides of the present invention may be incorporated into hybridization assay probes, capture probes and amplification primers, and used in various combinations thereof.

The invention relates to a real-time PCR (polymerase chain reaction) method for quintuply detecting target nucleic acid in a nucleic acid extracting solution in a single PCR reaction vessel, which is used for detecting Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium in a sample.

Disclosed are a DNA chip and a kit capable of quickly and accurately detecting or genotyping the highly prevalent and important eleven microbes causing sexually transmitted diseases (STD) Neisseria gonorrhoeae, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, syphilis-causing treponema, pallidum, chancroid-causing Haemophilus ducreyi, genital herpes-causing herpes simplex virus 1 and 2, human papillomavirus (HPV) and Trichomonas vaginalis and three related organisms Candida albicans, Gardnerella vaginalis and coliform bacteria and analyzing antibiotic resistance against tetracycline and lactam antibiotics, and a method for detecting or genotyping using the same. According to the present invention, the presence, genotype and antibiotic resistance of the fourteen organisms can be analyzed quickly and accurately from a DNA sample. With excellent sensitivity, specificity, reproducibility and accuracy of the 14 STD-causing and related microorganisms may be automatically identified quickly and accurately from multiple samples, and selection of antibiotics may be aided.

The invention relates to the detection of three different bacterial species which are responsible for sexually-transmitted diseases, i.e., Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG) and Mycoplasma genitalium (MG). The invention more particularly relates to the detection of these three species in real-time PCR, in multiplex PCR and in real-time multiplex PCR. The invention provides reference templates sequences, which are especially adapted to the design of primers and probes, which can be used together in the same tube to detect CT and/or MG and/or NG by real-time multiplex amplification.

The invention relates to genitourinary tract microorganism cultivation and detect method thereof, in particular to a genitourinary tract ureaplasma urealyticum (Uu) and mycoplasma hominis (Mh) culture medium and a detect method thereof. The culture medium comprises a fluid medium and a solid medium which are combined when the medium is used for culturing, separating and differentiating mycoplasma. The formulation comprises essential elements such as tryptic soy broth, yeast extract, urea, arginine, L-cysteine, phenol red, HEPES liquid, calf (fetal calf) serum, manganese sulfate, vancomycin, amphotericin B, trimethoprim, polymyxin, penicillin, and the like, and thereby the nutrition for mycoplasma growth and the antibiotic combination for inhibiting undesired microbe growth are optimized, no millipore filter is needed for sample inoculation and subcultivation, and the mycoplasma grows quickly. The fluid medium can maintain and prolong the logarithmic phase and indicate the growth of the mycoplasma, and the solid medium can used for observing Uu and Mh bacterial colonies, which has large background reflectance, thereby being easy for differentiation and significantly increasing the sensibility and the specificity of genitourinary tract mycoplasma detect.

The invention discloses a mycoplasma nucleic acid isothermal amplification method. In particular, the mycoplasma nucleic acid isothermal amplification method includes the step that amplification is carried out in a reaction system, wherein the reaction system comprises specific primer pairs for amplifying mycoplasmas, and primers can be used for amplifying amplification products corresponding to mycoplasma feature sequences from a detection sample with an extra-low mycoplasma copy number. By means of the mycoplasma nucleic acid isothermal amplification method, the to-be-detected sample containing mycoplasma RNA can be rapidly amplified with high specificity, high flexibility and low pollution; and the mycoplasma nucleic acid isothermal amplification method is particularly suitable for amplification and detection of the to-be-detected sample containing mycoplasma genitalium MG and/or mycoplasma pneumoniae MP. The mycoplasma nucleic acid isothermal amplification method has the beneficial effects of being high in detection efficiency and high in accuracy.

The invention provides a fluorescent PCR primer, probe and detecting kit for detecting mycoplasmas genitalium. According to the fluorescent PCR primer, probe and detecting kit for detecting the mycoplasmas genitalium, by comparing the genomes of all known mycoplasmas genitalium in the prior art, a multicopy repetitive sequence gene with the sequence shown as SEQ ID NO.1 is screened out, and the real-time fluorescent PCR primer and a probe of the multicopy repetitive sequence gene, which separately have nucleotide sequences shown as SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4, are designed. The detecting kit can reach a mycoplasma genitalium detecting sensitivity as low as 0.5 copies, have no specific amplification and present good specificity for 10 other common mycoplasmas and 7 common mycoplasmas genitalium, and have the advantages of being accurate in detection, high in sensitivity and specificity, simple and rapid, thereby achieving high specimen detecting ability.

The invention relates to an isolation medium of genitourinary tract mycoplasma Un and Mh and application of the isolation medium. Each 1,000ml of culture medium contains the following components: 15g of peptone, 15g of tryptone, 5g of sodium chloride, 5g of urea, 5g of arginine, 10ml of 0.4% phenol red, 50ml of bovine serum, 50-150ml of DMEM, 5-15ml of self-made biological factor and pure water added to a constant volume of 1,000ml. Compared with the prior art, according to the culture medium disclosed by the invention, due to the addition of an additive, the performance and the selectivity of the culture medium are improved; the nutrients are relatively abundant; the growth and reproduction requirements of Uu and Mh are easily met; and the result is observed and reported a day earlier in comparison with a traditional culture medium in general condition.

The invention relates to a urogenital tract infection pathogen multi-detection primer group and a detection device comprising the same. The primer group comprises a bottom piece and a cover piece, a first reagent storage groove, a second reagent storage groove and a connection passage are arranged on the bottom piece, the tail end of the second reagent storage groove is connected with an equalization passage, an exhaust system is arranged on the inner side of the equalization passage, and the first reagent storage groove, the connection passage, the second reagent storage groove and the equalization passage are connected with the exhaust system; equalization holes are formed along the equalization passage, and a sealing hole, a second buffer hole and a detection hole which are connected are formed in the radial direction of the equalization holes. One or multiple of Chlamydia trachomatis primer group, a gonococcus primer group, a ureaplasma urealyticum primer group, mycoplasma hominisprimer group and mycoplasma genitalium primer group and an internal reference primer group are contained in the detection holes respectively; an amplification reagent is contained in the second reagent storage groove or the detection holes. By the urogenital tract infection pathogen multi-detection primer group and the detection device, multi urogenital tract infection pathogen detection can be performed, single-sample multi-index simultaneous detection can be realized, and detection flux is increased.

The invention relates to the technical field of medical detection, in particular to primers for detecting mycoplasma genitalium, a probe for detecting mycoplasma genitalium, a kit for detecting mycoplasma genitalium and a detection method. The primers and the probe comprise a primer pair of nucleotide sequences shown in SEQ ID NO:1-2 as shown in the description and a probe of a nucleotide sequenceshown in SEQ ID NO:3 as shown in the description. The provided primers and probe can specifically bound to a mycoplasma genitalium outer membrane protein B gene and a human beta-globin gene, the detection sensitivity and specificity are significantly improved, the positive rate of low-value samples reaches 100%, and detection results are accurate and reliable so that the primers, probe and kit for detecting mycoplasma genitalium and the detection method can be applied to clinical detection of mycoplasma genitalium.

The present invention relates to a culture medium specific for microorganisms of Mycoplasma genus and, in particular, for species of hardly cultivable Mycoplasma (Mycoplasma genitalium, Mycoplasma pneumoniae, Mycoplasma penetrans, Mycoplasma fermentans, Mycoplasma pirium and/or Mycoplasma hyorhinis). The present invention also provides an in vitro method for the growth and/or isolation and/or identification of species belonging to the Mycoplasma genus as well as growth supports using such culture medium.

The invention discloses a method for detecting genital tract mycoplasmas based on loop-mediated isothermal amplification and a micro-fluidic chip and belongs to the technical field of pathogen detection and diagnosis. The method comprises the following steps: performing primer designing; manufacturing the micro-fluidic chip; preparing an isothermal amplification reaction system; supplying a sampleto the chip; and performing nucleic acid amplification, and judging and reading results. An isothermal amplification technique and a micro-fluidic chip technique are used in the method, polymerase with a chain replacement function is reacted under an isothermal condition of 63 DEG C, real-time fluorescence detection is implemented by using a fluorescence dye mixing method, and a sample with amplification positive can generate an ''S''-shaped amplification curve similar to real-time fluorescence. The micro-fluidic chip successfully prepared for four micro-fluidic chips has good sensitivity, specificity and accuracy, and the whole detection process can be completed within 1 hour. The minimum detection limits of Uu, Mg and Mh are 1000 copies/[mu] L, the minimum detection limit of Up is 5000copies/[mu] L, and the method has wide application prospects in clinical use.

The invention discloses a mycoplasma genitalium nucleic acid real-time fluorescence PCR detection primer, a probe and a kit. The kit comprises an amplification reaction solution, a mycoplasma genitalium nucleic acid detection solution, a positive reference substance and a negative reference substance, wherein the mycoplasma genitalium nucleic acid detection liquid comprises specific primers and probes aiming at mycoplasma genitalium and a human RNase P gene, the sequences of the primers aiming at the mycoplasma genitalium are as shown in SEQ ID NO.1 and SEQ ID NO.2, and the sequence of the probe aiming at the mycoplasma genitalium is as shown in SEQ ID NO.3. According to the primer, the probe and the kit for real-time fluorescence PCR detection of the mycoplasma genitalium, provided by the invention, the mycoplasma genitalium can be rapidly, accurately and sensitively detected, the repeatability of an experimental result is good, the precision is high, the detection time period is short, and the detection can be completed within 70 minutes as short as possible. Therefore, the detection time is greatly saved, and the clinical diagnosis efficiency can be accelerated.

The invention provides a primer group for detecting specific typing sites of eight genital tract pathogenic bacteria and two human papilloma viruses by a time of flight mass spectrometry method, a product and a non-diagnostic detection method. The genital tract pathogenic bacteria comprise staphylococcus aureus, group B streptococcus, neisseria gonorrhoeae, gardnerella vaginalis, candida albicans, mycoplasma genitalium, mycoplasma urealyticum, chlamydia trachomatis, and HPV16 and HPV18 virus types. The invention provides a novel method for detecting 10 kinds of genital tract pathogens by combining multiple PCR with clinical mass spectrometry for the first time, integrates technologies of multiple PCR, single base extension, mass spectrometry detection and the like, can amplify a detection template through the PCR technology, can detect a trace sample through the mass spectrometry technology, integrates the advantages of the two technologies, is much superior to the method for detecting specific fragments of pathogens by singly using PCR, and has higher detection sensitivity.

The invention relates to a quick detection test paper for ureaplasma urealyticum (uu) neutralization, in particular to a quick detection test paper used for uu neutralization after a large amount of clinical antibiotics is used and a preparation method thereof. The quick detection test paper for neutralization can detect out the positive result of genital tract mycoplasma after the large amount of clinical antibiotics is used, thereby having the function of the clinical diagnosis guidance and the clinical treatmen and the significant meaning of preventing the abuse of antibacterial drugs is. The quick detection test paper for uu neutralization is rich in nutrition and easy to prepare, has satisfied clinical application effect, solves the difficulty of bedside inoculation, is less polluted, has simple detection method, short time, strong selectivity, sensitive color change, high detection rate and good stability, can obtain a liquid for filtration enrichment and achieve the effects of permanently observing the growth condition of a colony after a sample is inoculated for 24 hours and can practically and reliably neutralize the corresponding clinical antibiotics; the products of reaction of the quick detection test paper for uu neutralization and the antibiotics do not sterilize experimental uu but inhibit the growth of the experimental uu; and the quick detection test paper for uu neutralization can rapidly and accurately detect the uu after a large amount of clinical antibiotics is used.

The invention provides a preparation method of a nucleic acid fingerprint database for detecting specific sites of eight genital tract pathogenic bacteria and two human papilloma virus types by a time-of-flight mass spectrometry. The genital tract pathogenic bacteria comprise staphylococcus aureus, group B streptococcus, neisseria gonorrhoeae, gardnerella vaginalis, candida albicans, mycoplasma genitalium, mycoplasma urealyticum, chlamydia trachomatis and virus types of HPV16 and HPV18 . The invention provides a novel method for detecting 10 genital tract pathogens by combining multiple PCR with clinical mass spectrometry for the first time, integrates multiple PCR, single base extension, mass spectrometry detection and other technologies, and establishes the preparation method of the nucleic acid fingerprint database, so that the model can amplify a detection template through the PCR technology, also can be used for detecting trace samples through the mass spectrometry technology, integrates the advantages of the two technologies, is far superior to a method for detecting a specific fragment of the pathogens by singly using PCR (Polymerase Chain Reaction), and has relatively high detection sensitivity.