PCR method of multiple sex propagate pathogene synchronous detection and kit

Abstract

The invention discloses a single-tube multiple PCR method for synchronous rapid detection of five sex propagate pathogenes (neisseria gonorrhoeae (NG), mycoplasma hominis (MH), mycoplasma genitalium (MG), chlamydia trachomatis (CT) and ureaplasma urealyticum (UU)) and a kit. The method is characterized by amplifying the five pathogenes and performing gel electrophoresis separation and detection by reasonably designing a primer and optimizing the primer concentration combination and PCR condition in the same reaction tube and under the same heat cycle condition. The invention has the characteristics of sensitivity, rapidness, convenience, and the like of the classic PCR, mainly realizes synchronous detection of the five pathogenes, has lower cost, can be used for developing relative kits and is used for clinical diagnosis and epidemiological survey and control.

Description

[0001] A PCR method, kit composition and application for rapid detection of five sexually transmitted disease pathogens (NG, UU, CT, MG and MH) by single-tube multiplex PCR technology technical field [0002] The invention relates to a PCR method and a kit for rapidly detecting five sexually transmitted disease pathogens, which are suitable for rapid clinical diagnosis and epidemiological investigation of sexually transmitted diseases. Background technique [0003] Sexually transmitted disease (Sexually Transmitted disease, STD) is a common and frequently-occurring disease at present. The traditional detection methods of sexually transmitted diseases mainly include: pathogen isolation and biological tests, electron microscope technology, immunofluorescence technology, fluorescent quantitative PCR technology, etc. These methods play an important role in disease diagnosis, but there are obvious deficiencies generally. For example, the cycle is long, the operation is cumbersome...

AI Technical Summary

Problems solved by technology

The traditional detection methods of sexually transmitted diseases mainly include: pathogen isolation and biological tests, electron microscope technology, immunofluorescence technology, fluorescent quantitative PCR technology, etc. These methods play an important role in disease diagnosis, but there are obvious deficiencies generally. Such as long cycle, cumbersome operation, only one or two pathogens can be detected at a time

At present, the infection rate of multiple mixed sexually transmitted diseases has increased significantly (Shen Jianwei, Sun Xiuqin, Cheng Donge. Investigation of mixed infection of female reproductive tract sexually transmitted disease pathogens. Chinese Journal of Hospital Infection [j], 2007, (05).), widely used Fluorescent quantitative PCR technology can only detect one pathogen at a time. If there is a sample, to detect whether it contains one or more of the above pathogens, it is necessary to perform multiple PCR reactions separately, and the detection cost is high. and diagnosis have brought difficulties, and experimental diagnostic techniques have lagged behind significantly

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