365 results about "Mixed infection" patented technology

The invention relates to a process for preparing antiviral or antibiotic essence for the prevention and treatment of bacterial and virus mixed infectious diseases of livestock, fowl and aquatic animals, which consists of mixing Chinese herbs of Bupleurum root, rhubarb horsetails, Coptis chinensis, baikal skullcap root, Chinese angelica root, clerodendroa cyrtophyllum and cordate houttuynia, lixiviating with 80-90% alcoholic solution, charging one or more antiviral or antibiotic elements.

The purpose of the invention is to disclose a porcine circovirus, porcine parvovirus and porcine reproductive and respiratory syndrome virus triplex virus-like particle vaccine and its preparation method. The triple virus-like particle vaccine (Triple VLP vaccine) of the invention contains VLP which is composed of PCV-2 major structural protein CAP protein, PPV VP2 protein epitope and PRRSV Gp5 protein epitope. It is proved by experiment that the vaccine can stimulate good double cellular and humoral immune response. It is shown by pharmacodynamic test that after immunization of different animal groups, the vaccine of injection, nose drops and water forms prepared by VLP antigen formed by the method with or without adjuvants can safely and effectively prevent the infection of PCV-2, PPV and PRRSV. The invention provides an ideal vaccine for the security of sows, piglets and fattening pigs to effectively prevent mixed infection of PCV-2, PPV and PRRSV.

The present invention refers to a treatment for mixed infectious diseases in the human reproductive system, wherein an association of compounds containing fluconazole and either tinidazole is used, the same being associated in doses lower to those commonly used therapeutically. The combination has proven to be highly efficacious and shown a good degree of tolerance.

The invention provides an amphimorphic FQ-PCR detection reagent kit for identifying African swine fever and swine fever virus wild strains. A P72 gene of ASFV and a 5'UTR noncoding region of CSFV arerespectively used as an amplification target area, a pair of specific primers and a TaqMan MGB probe (SEQ ID NO:1-6) are designed, a real-time fluorescent quantitation PCR(FQ-PCR) technique is used, and identification and detection of ASFV and CSFV are realized. The detection reagent kit provided by the invention is suitable for detecting viral nucleic acid in samples of serum, spleen, lymph nodes, tonsil, kidney and the like of suspected ASFV or CSFV infected pigs, the sensitivity can reach 1.0*10<1>copy/[mu]L, and the detection reagent kit does not have any cross reactions with other pathogens which are liable to be in mixed infection with the ASFV and the CSFV or of which the infection symptoms are similar such as PRRSV, PRV, PCV2, PPV, JEV and HPS.

The invention provides a combined inactivated vaccine against mycoplasma hyopneumoniae (MHP) and mycoplasma hyorhinis. The combined inactivated vaccine contains the MHP and the mycoplasma hyorhinis with preferred content as well as a Carbomer adjuvant with concentration of 10% (V/V). The invention further provides a preparation method of the combined inactivated vaccine against the MHP and the mycoplasma hyorhinis. The preparation method comprises the following steps: preparing production strains; preparing bacterium solution for producing seedlings; and inactivating, concentrating and blending to obtain the vaccine. The combined inactivated vaccine has the advantages of strong specificity and good immunity, thus solving the problem of specific infection caused by the MHP in the current domestic breeding farm and obtaining the mycoplasma hyorhinis vaccine under a blank state at home and abroad at present. The combined inactivated vaccine has the beneficial effects that the step of vaccine inoculation is simplified, trouble caused by a plurality of inoculation and easily produced side effects are avoided, and vaccine cost is saved, thus being especially applicable to preventing andtreating mixed infection diseases in the breeding farms at home and abroad and the like; and compared with the existing single vaccine, application range is widened and immune effect is enhanced.

The invention relates to the technical field of antibacterial gel, in particular to chitosan antibacterial gel and a preparation method thereof. Each liter of the chitosan antibacterial gel comprises the following raw materials by weight: 5-30g of modified chitosan, 2.5-10g of carbomer, 1-1.8g of alum, 2-40g of glycerol, 0.3-10g of lactic acid, 0.1-0.8g of menthol and 2-8g of sodium carboxymethylcellulose. The chitosan antibacterial gel which is good in curative effect, can be used for preventing relapse of inflammation and is harmless to human body is prepared by using the synergistic effect of modified chitosan serving as a major raw material as well as carbomer, alum, glycerol, lactic acid and sodium carboxymethylcellulose serving as auxiliary materials, and is suitable for preventing, treating and nursing various vaginal bacterial infections, trichomonas infections, mixed infections, vulvitis, leucorrhea, candidal infection as well as wound infections of cervix uteri, genital tract and mucous membrane. By using the chitosan antibacterial gel, wound healing after physical treatment of cervical erosion is promoted, and nursing of vaginal dryness as well as cross infections in sexual intercourse and the like is improved.

The invention discloses a method for preparing a bivalent yolk antibody for preventing and treating duck virus hepatitis I and III. The bivalent yolk antibody can be used for effectively preventing and treating the duck virus hepatitis I and III single and mixed infection and solving the problems that the existing vaccines, serum antibodies and yolk antibodies are not effective to the mixed infection. In addition, the bivalent yolk antibody is extracted by adopting a caprylic acid precipitation method. The method is safe, low in cost and suitable for mass production. The prepared bivalent yolk antibody has high purity and high titer, is stable in nature and is easy for storage.

The invention provides a double FQ-PCR detection kit for identifying PCV (porcine circoviruses) type 2 and type 3. By aiming at the characteristic of low Rep protein and Cap protein gene homology of PCV2 and PCV3, a pair of specific primers and a TaqMan MGB probe (SEQ ID NO:1-6) are designed by using Rep protein gene of PCV2 and Cap protein gene of PCV3 as amplification target regions; the real-time fluorescence quantitative PCR technology is used; the identification detection of the PCV2 and the PCV3 is realized. The detection kit provided by the invention is applicable to viral nucleic aciddetection in samples such as nose swab, oral cavity secreta, faeces, serum, lungs, lymph gland and kidneys of a suspected PCV infectious pig; the sensitivity can reach 1.0*10<1> copy/mu L; the crossedreaction with other pathogene capable of generating mixed infection or with similar of infection symptoms do not exist.

The invention relates to a compound sulfamonomethoxine sodium injection for veterinary use and a preparation method thereof, and belongs to the technical field of medicines for veterinary use. The raw material components of the compound sulfamonomethoxine sodium injection for veterinary use comprise sulfamonomethoxine sodium, dexamethasone sodium phosphate, trimethoprim, imidocarb, astragalus polysaccharide, organic solvent and water for injection. As a special compound preparation for veterinary use, the injection has remarkable efficacy on strengthening resistance of livestock and poultry, and treating mixed infection and severe diseases caused by a plurality of pathogenic bacteria and viruses. The injection has the advantages of convenient use, short treatment course, low drug resistance and the like.

The invention relates to traditional Chinese medicine for preventing and curing livestock and poultry virosis, which comprises and is made from the following raw materials by weight proportion: 8-12 proportions of goldthread root, 6-10 proportions of flos lonicerae, 8-12 proportions of forsythiae, 10-15 proportions of manyflower glorybower root, 10-15 proportions of dandelion, 5-8 proportions of liquorice, 10-15 proportions of lespedeza, 5-8 proportions of atractylodes sinensis and 10-15 proportions of grifola. The combined use of medicines of the invention can prevent and cure livestock and poultry virosis safely and effectively, eliminate accumulation of viruses in livestock and poultry, promote growth of livestock and poultry, and has particular effect in preventing and curing of hog plague, foot-and-mouth disease, angiostrongyliasis, Gongxingti, streptococcus, mildew, oedema, heart disorderly inflammation and mixed infection, wherein, the preventing rate is as high as over 95 percent and the recovery rate is as high as 90 percent.

The invention discloses a kit and a nucleotide sequence for inspecting three fish rhabdoviruses, which belongs to the field of inspection and quarantine. An oligonucleotide sequence for inspecting three fish rhabdoviruses is the oligonucleotide sequence shown from a sequence table SEQ ID No.1 to a sequence table SEQ ID No.9. The invention has the advantages that real-time fluorescent RT-PCR technique applied to the inspection of IHNV, VHSV and SVCV further improves the specificity and sensitivity of the inspection, reduces workload, improves work efficiency, and can perform quantitative inspection to a target fragment. Through the optimization of reaction conditions, the triple real-time fluorescent RT-PCR can inspect the virus quantity of 10<2> copy/reaction at least. The method also inspects virus mixed infection EPC (Epithelioma papulosum cyprini) cells and virus-challenge brachydanio rerio. Experimental results show that the method is a specific sensitive efficient virus inspection method. The technique has great application prospects in import-export sample quarantine, as well as the monitoring and diagnosis for clinical plague.

The present invention relates to a hemophilus parasuis disease, a swine streptococcosis bivalent inactivated vaccine and a preparation method thereof. The vaccine is prepared by adopting a hemophilus parasuis serotype 4 JS strain, a hemophilus parasuis serotype 5 ZJ strain and a streptococcus suis type 2 SC strain as antigens, and adopting a veterinarily acceptable adjuvant, concurrently provides immunoprophylaxis for hemophilus parasuis diseases caused by hemophilus parasuis, swine streptococcosis caused by streptococcus suis, and mixed infection of the hemophilus parasuis disease and the swine streptococcosis, has effects of low side effect, no endotoxin, no impurity protein in serum, good immunization safety, multi-prevention effect with one needle in the clinic, and cost reducing, and can meet different requirements of different users.

The invention discloses a Chinese medicinal herb preparation for poultry. The effective constituents of a Chinese medicinal herb product disclosed by the invention are made up of echinacea purpurea, honeysuckle, hypericum perforatum, glycyrrhiza, folium isatidis, platycodon grandiflorum, lophatherum gracile, mint and hypericin. The Chinese medicinal herb preparation disclosed by the invention can relieve the exterior syndrome with drugs pungent in flavor and cool in property and clear away heat and toxic materials; and the main therapeutical effects include strengthening vital Qi and eliminating pathogens, balancing Yin and Yang, and improving non-specific immunity of organisms. The Chinese medicinal herb preparation for poultry has significant therapeutical effect for depressed spirit of poultry, feed intake decrease, egg laying reduction, eyelid swelling of poultry caused by unknown viruses, respiratory inflammation, yellow-green dung, weight increment decline, soft-shelled eggs, and death rate increment, etc.; furthermore, the Chinese medicinal herb preparation for poultry can be utilized to prevent the diseases of untypical Newcastle disease and avian influenza etc. and treat the relevant mixed infection, secondary infection, untypical diseases and chronic cases; and finally, the Chinese medicinal herb preparation for poultry well promotes the recovery of production performance after the poultry is healed.

The Nano woman's cleaning and nursing aloe lotion contains nano aloe juice, sodium hyaluronate and polymerized material. The cleaning and nursing aloe lotion is prepared through crushing and atomizing aloe leaf inside a homogenizer to form nano grains of 1-700 nm and nano aloe suspension; and mixing the nano aloe suspension with sodium hydraluronate, polymerized material and substrate. The present invention has unique biological slowly releasing function, can repair and protect vagina and uterus wall, nourish vagina, eliminate discomfort in vagina, inhibit various vaginitis, cervical erosino and sexually transmitted diseases, eliminate inflammation and prevent cross infection. The present invention has no irritation, no toxicity and no side effect.

The invention discloses a multiple RT-PCR quick detection primer group for five porcine enteroviruses. The primer group comprises five pairs of PCR primers which respectively aim at the five porcine enteroviruses PEDV, TGEV, PDCoV, PRoV and PKV and respectively have base sequences shown in a sequence table from SEQ. ID. NO.1 to SEQ. ID. NO.10. According to this, the inventor further develops a corresponding quick detection kit. The primer group has the advantages of high specificity, high sensitivity, short time consumption and low cost when being applied in a same reaction tube to detect single or mixed infection of the five porcine enteroviruses at the same time. A quick, simple and convenient tool is provided for detection of swinery mixed infection, a solid foundation is laid for clinical quick identification and detection and lab epidemiological investigation, timely formulating of treatment plans in porcine industry is facilitated, and swinery death rate and economic loss are reduced.

The invention relates to a pathogenic microorganism analysis and identification system and application thereof, and belongs to the technical field of gene detection and analysis. The pathogenic microorganism analysis and identification system comprises: a data acquisition module used for acquiring gene sequencing data obtained by high-throughput sequencing; a data filtering module used for performing low-quality sequence filtering and host sequence filtering in sequence; a data comparison module used for comparing sequences to a pathogenic microorganism genome database; a species comparison module used for counting to-be-analyzed sequences; a data analysis module used for calculating the similarity S and the average similarity value SMSi of each species on each sequence comparison in the common comparison sequence set; a species sequence module used for calculating the total comparison sequence number SNTi of the species; and a result output module used for carrying out bioinformaticsanalysis to obtain a pathogenic microorganism analysis and identification result. The pathogenic microorganism analysis and identification system has the advantages of short analysis time and high accuracy, and can accurately detect mixed infection and obtain specific pathogen information.

The invention relates to hybridoma cells capable of secreting an anti-plasmodial lactate dehydrogenase monoclonal antibody, a monoclonal antibody and an application of the monoclonal antibody. The number of the hybridoma cells are three strains, are collected in Collection Center of Wuhan University in Luojiashan, Wuchang, Wuhan City of China Center for Type Culture Collection (CCTCC) and have collection numbers of CCTCC No: C2014185, CCTCC No: C2014184 and CCTCC No: C2014181. In addition, the invention also relates to a malaria test strip having high sensitivity and strong specificity and a malaria detection kit containing the malaria test strip. By the malaria detection kit, plasmodium falciparum can be accurately and quickly detected and the malaria detection kit has significant advantages of specificity and sensitivity compared to the traditional detection method and also can be applied in the detection of tertian malaria and mixed infection.

The invention discloses a quintuple RT-PCR detection method and kit for porcine epidemic diarrhea viruses, porcine transmissible gastroenteritis viruses, porcine rotaviruses, porcine sapoviruses and porcine kobuviruses. The kit comprises ten specificity amplification primers. In the using process of the kit, total RNA of a sample to be detected is subjected to reverse transcription to become cDNA through a 6-basic-group random primer, a detection reaction system in the kit is used for RCR amplification with the cDNA as a template, and whether the sample to be detected is infected with one kind of pathogens or is under mixed infection is determined according to different sizes of amplified PCR segments of different pathogens. On the condition of ensuring specificity and sensitivity, the kit has the advantages of being easy and quick to operate and lowering detection cost and labor intensity, and is suitable for field sample detection.

The invention discloses a reagent kit for identifying duck hepatitis A virus infection and novel duck reovirus infection. Specific primers (such as SEQ No. (sequence number) 1-4) of a duck hepatitis A virus and a novel duck reovirus are adopted to establish a double PCR (Polymerase Chain Reaction) detection method, and assembled into the reagent kit. The reagent kit can identify the duck hepatitis A virus and the novel duck reovirus in the same system and the mixed infection of the duck hepatitis A virus and the novel duck reovirus. The reagent kit has the characteristics of quickness, accuracy, high sensitivity, good specificity and the like, and can well solve the problem that the duck hepatitis A virus infection and the novel duck reovirus infection are difficult to distinguish clinically.

The invention relates to a veterinary compound sulfadiazine sodium injection and a preparation method thereof. The veterinary compound sulfadiazine sodium injection comprises sulfadiazine sodium, enrofloxacin, flunixin meglumine, trimethoprim, dexamethasone sodium phosphate, an organic solvent and water for injection. The injection serving as a veterinary special compound preparation has a special treating effect on various infectious diseases, mixed infections and systemic infections of the digestive system, the respiratory system, the urinary system and the skin soft tissue caused by sensitive bacteria and mycoplasmas of oxen, swines, poultries, canines, cats and aquatic animals The injection has the advantages of convenient use, short period of treatment, low medicament resistance and the like.

The invention discloses a synchronizing detection PCR agent box (hepatitis B virus, human immunodeficiency virus, human breast tumor virus, lues helicoid, human cytomegalovirus, simple herpesvirus, white oidiomycin, urea-dissolving mycoplasma, gonotoxin and Chlamydi trachomatis), which consists of blood type and secretion stains type. The user adds predisposed sample in the enlarging pipe to start enlarging reaction, which finishes detecting work simply and rapidly. The invention can detect ten venereal disease causal agents, which is fit for customs quarantine, prevailing monitor and hospital screen examination.