80 results about "Mixed infection" patented technology

The invention discloses a urinary system infection pathogen and drug resistance gene detection system and a kit. The detection system comprises forward and reverse primers which are respectively used for detecting escherichia coli, staphylococcus aureus, acinetobacter baumannii, klebsiella pneumoniae, enterococcus faecium, candida glabrata, proteus mirabilis, enterococcus faecalis, candida tropicalis, enterobacter cloacae, candida albicans, pseudomonas aeruginosa and streptococcus agalactiae; the forward primer and the reverse primer are respectively used for detecting the blaCTX-M gene of the extended spectrum beta-lactamase, the forward primer and the reverse primer are used for detecting the blaSHV gene of the extended spectrum beta-lactamase, and the forward primer and the reverse primer are used for detecting the blaTEM gene of the extended spectrum beta-lactamase. According to the forward and reverse primers for detecting the blaNDM gene of the extended-spectrum beta-lactamase, a detection sample is urine. According to the invention, common gram-negative bacteria, gram-positive bacteria and fungi can be detected at the same time, and the problem of missed detection of mixed infection in traditional culture is solved.

The invention relates to the technical field of virus detection, in particular to a primer probe combination for bovine respiratory disease syndrome pathogen identification and application and a kit thereof. When the bovine viral diarrhea virus is identified, the nucleotide sequence of the upstream primer of the used primer probe group is as shown in SEQ ID No.1, the nucleotide sequence of the downstream primer is as shown in SEQ ID No.2, and the nucleotide sequence of the probe is as shown in SEQ ID No.3. The primer probe combination provided by the invention can be used for identifying and diagnosing eight common pathogens capable of causing the bovine respiratory disease syndrome at one time through a two-tube reaction system by using a fluorescent quantitative PCR method, can also be used for identifying a mixed infected sample, has the advantages of accurate identification, high sensitivity, simplicity in operation, short time consumption and the like, and can be used for detecting the bovine respiratory disease syndrome. And an effective technical means can be provided for pathogen diagnosis and disease prevention of the bovine respiratory disease syndrome in China.

The invention relates to the technical field of virus traceability, and particularly discloses a coronavirus sample detection method which comprises the following steps: S1, acquiring high-throughput original sequencing data of a target sample; s2, inputting the original sequencing data into a dynamic learning type recognition model, and outputting each virus pedigree and the credibility of each pedigree; s3, performing noise perception variation detection on the original sequencing data; s4, aiming at each virus lineage, determining a variation point; s5, detecting a mixed infection indicator; s5, constructing a Bayesian network to determine the genetic relationship among the variation points, and generating a virus haplotype sequence; s6, calculating a genetic distance, and deducing a propagation path through a maximum likelihood method; and outputting a traceability report. The method solves the problem of frequency conflict when multiple pedigree coexist, is suitable for the situation that multiple pedigree viruses coexist to form mixed infection or co-infection, and avoids misjudgment of attribution of variation sites.

The invention relates to compound herba houttuyniae eye drops as well as a preparation method and application of the compound herba houttuyniae eye drops. Comprising the following preparation raw materials in percentage by mass: 1-6% of a herba houttuyniae extracting solution, 1-5% of an aglaia odorata extracting solution, 1-2% of a gelidium amansii extracting solution, 0.1-0.3% of acyclovir, 0.1-0.3% of tobramycin, 0.01-0.1% of dexamethasone sodium phosphate, 0.1-1% of sodium hyaluronate, 0.1-3% of glycerol and 88-93% of purified water. According to the compound herba houttuyniae eye drops prepared by the preparation method disclosed by the invention, the herba houttuyniae extracting solution, the aglaia odorata extracting solution, the gelidium amansii extracting solution, the acyclovir, the tobramycin and the dexamethasone sodium phosphate are combined for use, so that the compound herba houttuyniae eye drops have a relatively good curative effect on pets suffering from bacterium and virus mixed infection type conjunctivitis / keratitis; the compound herba houttuyniae eye drops are good in high-temperature-resistant storage stability and good in curative effect, the problem of frequent administration can be reduced, and the compound herba houttuyniae eye drops are more convenient to use and store.

The invention belongs to the technical field of biology, and particularly relates to a multi-respiratory-tract pathogen mixed infection animal model and an establishment method and application thereof. The construction method provided by the invention comprises the following steps: firstly, infecting a female BALB / c mouse by using an influenza virus and mycoplasma pneumoniae virus mixture to construct a mycoplasma viral infection animal model, infecting for 20-28 hours, and finally successfully constructing a mixed infection mouse model. The state of the infected mouse is further observed, and the weight and death conditions, the clinical symptoms and tissue pathological changes of the infected animal model and the like are recorded. Therefore, the construction method provided by the invention is simple, and the constructed animal model is a safe, effective and economical tool and can be used for rapid preclinical evaluation of the screening process of medical products such as respiratory tract mixed infection detection kits, vaccines, medicines and the like under biosafety level 2 laboratory conditions.

The invention discloses an antibody as well as a preparation method and application thereof. The antibody has good binding activity with OXA-23 type carbapenem enzyme, and can reduce the carbapenem drug resistance level of the acinetobacter baumannii, so that the clinical use concentration of meropenem is reduced, and the treatment effect of meropenem on the acinetobacter baumannii is improved. In addition, the antibody disclosed by the invention can also inhibit OXA-23 protein secreted by bacteria, prevent the bacteria from hydrolyzing carbapenem antibiotics, increase the concentration of antibiotics in a medication part, and facilitate the removal of mixed infection with acinetobacter baumannii. The invention provides a new strategy for treating acinetobacter baumannii infection, and the application prospect is wide.

The invention relates to a quadruple TaqMan real-time fluorescent quantitative PCR (Polymerase Chain Reaction) primer group and a probe group for simultaneously detecting cGPV (Complementary Glutathione Virus), MDPV (Minimal Disease Papilloma Virus), MDGPV (Minimal Disease Papilloma Virus) and SBDSV (Sequence Broadcast DSV) and a kit thereof. The sequences of the primer group and the probe group are respectively shown as SEQ ID NO.1-12. The research successfully develops and verifies a multiple TaqMan-MGB real-time fluorescent PCR method capable of simultaneously detecting and distinguishing four important waterfowl parvoviruses (cGPV, MDPV, MDGPV and SBDSV). The method has good specificity, repeatability and high sensitivity. Compared with a conventional PCR method, the multiple detection method has the advantages that the clinical waterfowl parvovirus detection rate is remarkably improved, mixed infection can be effectively recognized, and the clinical diagnosis efficiency is greatly improved.

The invention discloses a detection reagent, a kit and a detection method for pathogens of micropterus salmoides. The micropterus salmoides pathogen detection reagent comprises primers and probes for specifically amplifying an infectious spleen and kidney necrosis virus, a viral nervous necrosis virus, flavobacterium columnar, a perch rhabdovirus, a micropterus salmoides double-RNA virus, edwardsiella tarda, nocardia and a micropterus salmoides iridovirus; specific upstream and downstream primers and probes of the eight pathogens are sequences as shown in SEQ ID NO.1 to SEQ ID NO.24 in sequence. The micropterus salmoides pathogen detection reagent and method can detect eight pathogens at the same time, have the advantages of high sensitivity, high specificity, good repeatability and the like, provide reliable basis for prevention and control of the pathogens, also improve the prevention and control work efficiency, and have good application prospects. The kit is especially suitable for large-scale rapid screening and mixed infection diagnosis of aquaculture pathogens, and has important value and significance for prevention and treatment of micropterus salmoides pathogens.

The application discloses application of a traditional Chinese medicine composition in preparation of a medicine for treating vaginitis, and preparation raw materials of the traditional Chinese medicine composition include zedoary turmeric and Chinese atractylodes rhizome. The traditional Chinese medicine composition can kill common pathogenic bacteria of vaginosis, and has good bacteriostatic effect. While inhibiting the common pathogenic bacteria of vaginosis, the traditional Chinese medicine composition does not inhibit lactobacillus of vagina. The traditional Chinese medicine composition can not only kill single common pathogenic bacteria of vaginosis, but also relieve trichomonas vaginalis, and inhibit mixed infection of bacteria and fungi.

The application discloses a kind of absolute quantitative detection method of child respiratory tract multi-pathogen based on multi-target ladder microarray and exogenous internal reference correction.The method sets up independent micro-reaction unit to different pathogen, adds fixed amount of exogenous internal reference nucleic acid, and calculates template number by positive unit proportion combined with Poisson distribution model, then corrects the result by using the theoretical addition amount of exogenous internal reference, so as to realize parallel detection and absolute quantification of pathogen such as bocavirus, adenovirus, mycobacterium tuberculosis and mycoplasma pneumoniae without standard curve and special digital PCR equipment.The application has the advantages of high sensitivity, strong anti-interference ability, suitable for mixed infection sample analysis and clinical application, etc.

The invention provides application of Hnf4alpha or a biological material containing Hnf4alpha in preparation of a product for inhibiting bacteria and / or viruses, and belongs to the technical field of biological medicines. In-vivo and in-vitro experiments show that overexpression of the Hnf4alpha not only can effectively prevent infection of aeromonas salmonicida and grass carp hemorrhagic disease viruses, but also can remarkably inhibit co-infection of the aeromonas salmonicida and the grass carp hemorrhagic disease viruses. The bacterial or virus load after infection is obviously reduced, and the activity of cells and the survival rate of juvenile fish are improved. The invention provides a molecular target for prevention and control of bacterial infectious diseases, virus infectious diseases and mixed infectious diseases of cyprinid fishes.

The attenuated strain of Bordetella bronchiseptica (YZ01-P strain) of the present invention is genetically homologous to the pathogenic Bordetella bronchiseptica prevalent in China. After immunizing animals with the vaccine composition prepared from the attenuated strain, it can induce the animal body to rapidly produce good mucosal immunity, and its immunological efficacy can be maintained for a long time. During the period of maintaining immunological efficacy, it can effectively prevent the occurrence of canine infectious respiratory diseases caused by infection with virulent strains of Bordetella bronchiseptica. At the same time, it can also be reasonably inferred that it can greatly reduce the occurrence of canine infectious diseases characterized by respiratory diseases caused by the mixed infection of virulent strains of Bordetella bronchiseptica with canine distemper, canine parainfluenza and canine adenovirus.

The invention relates to the technical field of biological medicines, discloses a broad-spectrum bacteriophage composition for preventing and controlling swine atrophic rhinitis and application of the broad-spectrum bacteriophage composition, and particularly relates to a pasteurella bacteriophage SD-PM1 and a bordetella bronchiseptica bacteriophage SD-Bb1, and the preservation numbers of the pasteurella bacteriophage SD-PM1 and the bordetella bronchiseptica bacteriophage SD-Bb1 are CCTCC NO: M20232599 and CCTCC NO: M20232589 respectively. The bacteriophage and the composition thereof have a strong broad-spectrum lysis effect on porcine pasteurella and porcine bordetella bronchiseptica, and the bacteriophage can be independently compounded with cocktail or compounded with cocktail, and can be used for preparing medicines for preventing and treating porcine respiratory diseases caused by independent infection or mixed infection of pasteurella and bordetella bronchiseptica. The traditional Chinese medicine composition especially has a remarkable effect on atrophic rhinitis of pigs. In addition, the bacteriophage provided by the invention is a strong bacteriophage, has a wide lysis spectrum, has good biological characteristics of acid resistance and heat resistance, and can also be applied to various actual scenes.

The invention is applicable to the technical field of PCR (polymerase chain reaction) detection, and provides an eperythrozoonosis and babesiosis dual rapid detection kit and application thereof, and an eperythrozoon and babesiosis specific primer group, which comprises an eperythrozoon upstream primer, an eperythrozoon downstream primer, a babesiosis upstream primer and a babesiosis downstream primer, the nucleotide sequence of the upstream primer of the eperythrozoon is as shown in SEQ ID No. 1, and the nucleotide sequence of the downstream primer of the eperythrozoon is as shown in SEQ ID No. 2; the nucleotide sequence of the upstream primer of the babesia is as shown in SEQ ID No.3, and the nucleotide sequence of the downstream primer of the babesia is as shown in SEQ ID No.4. The PCR primers are designed for specific gene areas of the two pathogens, optimization is carried out on targeting sites and sequences, and the PCR primers are remarkably different from conventional primers, have higher specificity and amplification pertinence and solve the problems that an existing detection method is poor in specificity and low in sensitivity and cannot synchronously detect the two pathogens. And rapid and accurate diagnosis of single or mixed infection of eperythrozoon and babesia is realized.

The application relates to the technical field of animal virus molecular detection, and particularly discloses a multiple detection method for ASFV, PCV2 and PCV3 suitable for rapid real-time fluorescent PCR. Specific primers and TaqMan probes are designed according to the B646L gene of ASFV and the ORF1 gene conservative regions of PCV2 and PCV3, and the sequences are shown as SEQ ID NO:1-9. The primers and probes are optimized: avoiding strong GC anchoring at the 3' end of the primers, continuous AT series and G base at the 3' end of the probes, and shortening the length of the probes to adapt to rapid real-time fluorescent PCR. The minimum detection limit of the three viruses is not higher than 5 copies per muL, the detection is completed within 30 minutes, there is no cross reaction with 11 common pig pathogenic agents, the coefficient of variation is less than 2%, the sensitivity is high, the specificity is strong, the method is rapid and convenient, and is suitable for rapid diagnosis of mixed infection of ASFV, PCV2 and PCV3.

The present invention provides a primer set for differentiating Mycobacterium tuberculosis complex and non-tuberculous mycobacteria and its application. The primer set includes the nucleotide sequences shown in SEQ ID NO: 1-118. The present invention collects and analyzes the identification genes of Mycobacterium tuberculosis complex and non-tuberculous mycobacteria from the database, designs specific primers for the identification genes, combines multiplex PCR technology and targeted high-throughput sequencing technology, improves the detection sensitivity by ultra-multiplex PCR amplification and enrichment of the target, and improves the detection specificity based on the sequence reading of the gene sequencing platform, so as to achieve accurate and sensitive differentiation of mycobacteria. It has low detection cost, short detection cycle, high sensitivity, good specificity, and can effectively identify mixed infections, and has important application value in the detection of tuberculosis / NTM diseases.

The invention provides a construction method and application of a chicken fallopian tube embolism model, and belongs to the technical field of animal infection models. The invention provides a construction method of a chicken fallopian tube embolism model based on multi-pathogen infection. The mycoplasma synoviae, the infectious bronchitis virus and the riemerella anatipestifer are utilized to infect the chicken, and the clinical typical salpingemphasis pathological process including cheese-like substance accumulation in a salpingemphasis cavity, serious abdominal air sacculitis complications and related tissue inflammation changes can be accurately reproduced. The model constructed by the invention is high in morbidity, and is suitable for epidemic disease pathogenesis research, drug screening and vaccine potency test. Compared with an existing single pathogen infection model, the model is closer to a clinical reality multi-pathogen cooperative infection scene, and the blank of a standardized multi-pathogen mixed infection induced fallopian tube embolism animal model is filled up.

The application belongs to the technical field of virus detection, and discloses a sheep disease virus multiplex PCR detection primer, method and application. The base sequence of the primer FMDV-F of the sheep foot-and-mouth disease virus is SEQ ID NO:1, the base sequence of the primer FMDV-R is SEQ ID NO:2; the base sequence of the primer ORFV-F of the sheep orf virus is SEQ ID NO:3, the base sequence of the primer ORFV-R is SEQ ID NO:4; the base sequence of the primer GTPV-F of the goat pox virus is SEQ ID NO:5, and the base sequence of the primer GTPV-R is SEQ ID NO:6. The application optimizes the multiplex PCR, determines the optimal reaction system and the optimal reaction procedure, and can accurately detect single or mixed infection of the sheep foot-and-mouth disease virus, the sheep orf virus and the goat pox virus, and has an important role in epidemiological investigation and epidemic disease prevention and control.

The present application belongs to the field of molecular biology detection, and particularly relates to a composition, method and use for simultaneous detection and differentiation of swine fever virus, psittacosis chlamydia, porcine reproductive and respiratory syndrome virus and porcine circovirus type II. The composition for simultaneous detection provided by the present application can realize rapid detection of four pathogen target genes in one tube of reaction solution by using multiplex fluorescence RPA detection technology, and has the advantages of high throughput, short time and low cost. On one hand, the present application can accurately detect pathogens and analyze mixed infection of pathogens, so as to achieve the effect of precise medication, reduce the use of antibiotics, save costs, and reduce the cost and increase the benefit of enterprises, and on the other hand, compared with fluorescence PCR, the detection time is greatly shortened, and the purpose of rapid detection is achieved.

The invention provides a primer probe combination, a kit and a method for synchronously detecting PCV2 and Mhp, and belongs to the technical field of animal epidemic disease molecular diagnosis. According to the technical scheme provided by the invention, the porcine circovirus type 2 and the mycoplasma hyopneumoniae can be simultaneously, rapidly and accurately detected in one reaction system, the operation steps are remarkably simplified, the detection efficiency is improved, and the detection cost is reduced. The method is high in sensitivity, and infection can be found early; the specificity is strong, the cross reaction is effectively avoided, and the result is reliable. The matched kit is convenient for standardized production and clinical popularization, provides an efficient and convenient technical tool for mixed infection monitoring of the two important pathogens in a pig farm and early diagnosis and precise prevention and control of epidemic situations, and has good application prospects and market values.

This invention relates to the field of intelligent identification technology for swine diseases, and discloses a method for identifying and making decisions about swine diseases by integrating body temperature and image features. The method separates the independent basis components of each disease in a mixed infection by performing non-negative matrix decomposition on the body temperature time spectrum. It then uses the Hungarian algorithm to establish a cross-time point correspondence between the basis components, and uses an optimal transmission algorithm to associate lesion instances with the body temperature basis components to generate a feature sequence of disease components. Finally, it uses a time-series perceptual graph neural network to perform independent inference on the symptom evolution time-series graph, outputting the disease type identification confidence and disease stage inference confidence for each pathogen. Based on a multi-pathogen joint decision rule base, it generates a coordinated treatment plan.

The invention relates to an engineered bacteriophage targeting drug-resistant gram-negative bacteria, a screening method and application. The engineered phage is Escherichia phage M13, the preservation time is February 11, 2025, the preservation address is China Center for Type Culture Collection (CCTCC), Wuhan University, Wuchang District, Wuhan City, Hubei Province, and the preservation number is CCTCC No: M 2025196. The invention also provides application of the engineered bacteriophage in preparation of medicines for treating and diagnosing multi-drug-resistant gram-negative bacterium infection. The invention also provides a kit for diagnosing or treating multi-drug-resistant gram-negative bacterium infection, and the kit comprises the bacteriophage. The invention also provides a screening method of the engineered bacteriophage. The invention solves the problem that the natural bacteriophage is targeted to single species of bacteria and is difficult to cope with common mixed infection of multiple drug-resistant bacteria clinically.

To provide an antiviral agent containing probiotics as an active ingredient that effectively exerts antiviral effects even in combined infections of viruses and pathogenic bacteria. [Solution] An antiviral agent is used that contains one or more strains of Lactobacillus bacteria having a 16S rRNA gene that is at least 90% identical to the nucleotide sequence shown in Sequence ID No. 1, and that has an effect of enhancing the expression of antiviral factors and / or reducing the expression of downregulatory factors of antiviral factors.

The present invention aims to provide potential drug candidates for multidrug-resistant bacterial infections. [Solution] We provide short-chain antimicrobial peptides that are rich in tryptophan and have self-assembly properties. These antimicrobial peptides consist of tryptophan (W) and a positively charged amino acid and have the ability to self-assemble. As broad-spectrum antimicrobial peptides, these antimicrobial peptides can be used to treat microbial infections, such as mixed infections caused by Pseudomonas aeruginosa and Staphylococcus aureus.

The invention provides a molecular diagnosis method for avian leukosis virus and Marek's disease virus mixed infection. The molecular diagnosis method comprises the following steps: (S01) collecting diseased material tissues; (S02) carrying out nucleic acid extraction on the sick material tissue of the sick chicken; (S03) detecting the nucleic acid by using specific primers of avian leukosis and Marek's disease; (S04) preparing a PCR (Polymerase Chain Reaction) detection system, and respectively adding ALV-env gene amplification primers and MDV-meq gene amplification primers for gene amplification; (S05) carrying out 1% agarose gel electrophoresis detection on the amplification product in the step (4), and carrying out sequence determination after determining a target band; (S06) carrying out BLAST comparison on the sequences obtained by sequencing, carrying out gene sequence analysis and drawing an evolutionary tree; and (S07) determining the virus type according to the homology and the evolutionary branches, so as to realize rapid diagnosis of ALV and MDV mixed infection, strain characteristic analysis and exclusion of other similar epidemic diseases, and provide technical support for accurate prevention and control of AL and MD in the poultry industry, provenance purification and epidemiological investigation.

The application discloses a traditional Chinese medicine feed additive for preventing and treating Simmental respiratory disease syndrome and a preparation method and application thereof, and belongs to the technical field of livestock feed additives. The additive is prepared from traditional Chinese medicine raw materials, such as ephedra, honeysuckle, forsythia, radix scrophulariae, radix houttuyniae, radix isatidis, platycodon, bitter apricot kernel, radix glycyrrhizae, radix astragali, rhizoma atractylodis, mint, loquat leaf and radix codonopsis, through decocting, extracting, low-temperature extracting after mint, concentrating and spray drying. The application adopts a three-step prevention and treatment strategy of 'dispelling lung-evil, clearing heat and resolving toxin and supporting healthy body', can effectively prevent and adjuvantly treat Simmental respiratory disease syndrome caused by mixed infection of multiple pathogens, significantly reduces the morbidity of calf BRDC, enhances the immune function of the body, promotes growth and weight gain, has the characteristics of no antibiotic residue, no drug resistance, good palatability and the like, and meets the requirements of green and healthy breeding.

The present invention relates to PD-0184264 for use in a method for the treatment of a viral infection or a viral and bacterial coinfection. Also provided are compositions comprising such inhibitors for use in the treatment of a virus infection and the prevention and / or treatment of a bacterial co-infection.

A problem of the invention is to provide an antiviral agent containing as an active ingredient a probiotic which effectively exhibits an antiviral effect also on combined infection with a virus and a pathogenic bacterium. An agent containing one, two or more Lactobacillus strains which have 16S rRNA gene having an identity of at least 90% with the nucleotide sequence of SEQ ID NO: 1 and which have the action of enhancing the expression of an antiviral factor and / or the action of reducing the expression of a downregulator of an antiviral factor is used as an antiviral agent.

The invention discloses a compound immunopotentiator as well as a preparation method and application thereof, and belongs to the technical field of aquaculture. The compound immunopotentiator contains a gallnut water extract, a gallnut alcohol extract, an astragalus membranaceus water extract, an astragalus membranaceus alcohol extract, a rhodiola rosea water extract, a rhodiola rosea alcohol extract and chlorogenic acid. The compound immunopotentiator provided by the invention can significantly improve the resistance of aquaculture animals to pathogen infection, is free of environmental pollution and drug resistance risks, and has good safety and effectiveness. Experiments show that for aquatic products infected with nervous necrosis viruses, the positive rate of the nervous necrosis viruses can be obviously reduced by adding the compound immunopotentiator into feed of the aquatic products; for aquatic products treated by mixed infection of nervous necrosis virus and vibrio harveyi, the death rate of the aquatic products can be obviously reduced by adding the compound immunopotentiator into feed of the aquatic products, so that the compound immunopotentiator disclosed by the invention has a good immunoprotection effect when coping with the mixed infection of virus and bacteria.

The invention discloses an RNA (Ribonucleic Acid) virus-mediated cucumber simple, rapid and efficient gene silencing technical system, the operation process of RNA virus inoculation is simplified, only an agrobacterium VIGS mixed infection solution containing ALSV1 and ALSV2 target gene silencing vectors needs to be prepared, seeds germinated by the inoculated cucumber are directly infected by utilizing a vacuumizing method, and the seeds are directly infected by utilizing an agrobacterium tumefaciens VIGS mixed infection solution containing ALSV1 and ALSV2 target gene silencing vectors. The cucumber seedling characterization verification gene function can be observed in 16-18 days, the cucumber gene silencing efficiency can reach 100% through the method, and a new path is developed for verification and evaluation of the cucumber gene function.