47 results about "Mixed infection" patented technology

The invention relates to the technical field of virus traceability, and particularly discloses a coronavirus sample detection method which comprises the following steps: S1, acquiring high-throughput original sequencing data of a target sample; s2, inputting the original sequencing data into a dynamic learning type recognition model, and outputting each virus pedigree and the credibility of each pedigree; s3, performing noise perception variation detection on the original sequencing data; s4, aiming at each virus lineage, determining a variation point; s5, detecting a mixed infection indicator; s5, constructing a Bayesian network to determine the genetic relationship among the variation points, and generating a virus haplotype sequence; s6, calculating a genetic distance, and deducing a propagation path through a maximum likelihood method; and outputting a traceability report. The method solves the problem of frequency conflict when multiple pedigree coexist, is suitable for the situation that multiple pedigree viruses coexist to form mixed infection or co-infection, and avoids misjudgment of attribution of variation sites.

The invention relates to compound herba houttuyniae eye drops as well as a preparation method and application of the compound herba houttuyniae eye drops. Comprising the following preparation raw materials in percentage by mass: 1-6% of a herba houttuyniae extracting solution, 1-5% of an aglaia odorata extracting solution, 1-2% of a gelidium amansii extracting solution, 0.1-0.3% of acyclovir, 0.1-0.3% of tobramycin, 0.01-0.1% of dexamethasone sodium phosphate, 0.1-1% of sodium hyaluronate, 0.1-3% of glycerol and 88-93% of purified water. According to the compound herba houttuyniae eye drops prepared by the preparation method disclosed by the invention, the herba houttuyniae extracting solution, the aglaia odorata extracting solution, the gelidium amansii extracting solution, the acyclovir, the tobramycin and the dexamethasone sodium phosphate are combined for use, so that the compound herba houttuyniae eye drops have a relatively good curative effect on pets suffering from bacterium and virus mixed infection type conjunctivitis / keratitis; the compound herba houttuyniae eye drops are good in high-temperature-resistant storage stability and good in curative effect, the problem of frequent administration can be reduced, and the compound herba houttuyniae eye drops are more convenient to use and store.

The invention discloses an antibody as well as a preparation method and application thereof. The antibody has good binding activity with OXA-23 type carbapenem enzyme, and can reduce the carbapenem drug resistance level of the acinetobacter baumannii, so that the clinical use concentration of meropenem is reduced, and the treatment effect of meropenem on the acinetobacter baumannii is improved. In addition, the antibody disclosed by the invention can also inhibit OXA-23 protein secreted by bacteria, prevent the bacteria from hydrolyzing carbapenem antibiotics, increase the concentration of antibiotics in a medication part, and facilitate the removal of mixed infection with acinetobacter baumannii. The invention provides a new strategy for treating acinetobacter baumannii infection, and the application prospect is wide.

The invention discloses a detection reagent, a kit and a detection method for pathogens of micropterus salmoides. The micropterus salmoides pathogen detection reagent comprises primers and probes for specifically amplifying an infectious spleen and kidney necrosis virus, a viral nervous necrosis virus, flavobacterium columnar, a perch rhabdovirus, a micropterus salmoides double-RNA virus, edwardsiella tarda, nocardia and a micropterus salmoides iridovirus; specific upstream and downstream primers and probes of the eight pathogens are sequences as shown in SEQ ID NO.1 to SEQ ID NO.24 in sequence. The micropterus salmoides pathogen detection reagent and method can detect eight pathogens at the same time, have the advantages of high sensitivity, high specificity, good repeatability and the like, provide reliable basis for prevention and control of the pathogens, also improve the prevention and control work efficiency, and have good application prospects. The kit is especially suitable for large-scale rapid screening and mixed infection diagnosis of aquaculture pathogens, and has important value and significance for prevention and treatment of micropterus salmoides pathogens.

The application discloses application of a traditional Chinese medicine composition in preparation of a medicine for treating vaginitis, and preparation raw materials of the traditional Chinese medicine composition include zedoary turmeric and Chinese atractylodes rhizome. The traditional Chinese medicine composition can kill common pathogenic bacteria of vaginosis, and has good bacteriostatic effect. While inhibiting the common pathogenic bacteria of vaginosis, the traditional Chinese medicine composition does not inhibit lactobacillus of vagina. The traditional Chinese medicine composition can not only kill single common pathogenic bacteria of vaginosis, but also relieve trichomonas vaginalis, and inhibit mixed infection of bacteria and fungi.

The application discloses a kind of absolute quantitative detection method of child respiratory tract multi-pathogen based on multi-target ladder microarray and exogenous internal reference correction.The method sets up independent micro-reaction unit to different pathogen, adds fixed amount of exogenous internal reference nucleic acid, and calculates template number by positive unit proportion combined with Poisson distribution model, then corrects the result by using the theoretical addition amount of exogenous internal reference, so as to realize parallel detection and absolute quantification of pathogen such as bocavirus, adenovirus, mycobacterium tuberculosis and mycoplasma pneumoniae without standard curve and special digital PCR equipment.The application has the advantages of high sensitivity, strong anti-interference ability, suitable for mixed infection sample analysis and clinical application, etc.

The invention is applicable to the technical field of PCR (polymerase chain reaction) detection, and provides an eperythrozoonosis and babesiosis dual rapid detection kit and application thereof, and an eperythrozoon and babesiosis specific primer group, which comprises an eperythrozoon upstream primer, an eperythrozoon downstream primer, a babesiosis upstream primer and a babesiosis downstream primer, the nucleotide sequence of the upstream primer of the eperythrozoon is as shown in SEQ ID No. 1, and the nucleotide sequence of the downstream primer of the eperythrozoon is as shown in SEQ ID No. 2; the nucleotide sequence of the upstream primer of the babesia is as shown in SEQ ID No.3, and the nucleotide sequence of the downstream primer of the babesia is as shown in SEQ ID No.4. The PCR primers are designed for specific gene areas of the two pathogens, optimization is carried out on targeting sites and sequences, and the PCR primers are remarkably different from conventional primers, have higher specificity and amplification pertinence and solve the problems that an existing detection method is poor in specificity and low in sensitivity and cannot synchronously detect the two pathogens. And rapid and accurate diagnosis of single or mixed infection of eperythrozoon and babesia is realized.

The application relates to the technical field of animal virus molecular detection, and particularly discloses a multiple detection method for ASFV, PCV2 and PCV3 suitable for rapid real-time fluorescent PCR. Specific primers and TaqMan probes are designed according to the B646L gene of ASFV and the ORF1 gene conservative regions of PCV2 and PCV3, and the sequences are shown as SEQ ID NO:1-9. The primers and probes are optimized: avoiding strong GC anchoring at the 3' end of the primers, continuous AT series and G base at the 3' end of the probes, and shortening the length of the probes to adapt to rapid real-time fluorescent PCR. The minimum detection limit of the three viruses is not higher than 5 copies per muL, the detection is completed within 30 minutes, there is no cross reaction with 11 common pig pathogenic agents, the coefficient of variation is less than 2%, the sensitivity is high, the specificity is strong, the method is rapid and convenient, and is suitable for rapid diagnosis of mixed infection of ASFV, PCV2 and PCV3.

The invention provides a construction method and application of a chicken fallopian tube embolism model, and belongs to the technical field of animal infection models. The invention provides a construction method of a chicken fallopian tube embolism model based on multi-pathogen infection. The mycoplasma synoviae, the infectious bronchitis virus and the riemerella anatipestifer are utilized to infect the chicken, and the clinical typical salpingemphasis pathological process including cheese-like substance accumulation in a salpingemphasis cavity, serious abdominal air sacculitis complications and related tissue inflammation changes can be accurately reproduced. The model constructed by the invention is high in morbidity, and is suitable for epidemic disease pathogenesis research, drug screening and vaccine potency test. Compared with an existing single pathogen infection model, the model is closer to a clinical reality multi-pathogen cooperative infection scene, and the blank of a standardized multi-pathogen mixed infection induced fallopian tube embolism animal model is filled up.

The application belongs to the technical field of virus detection, and discloses a sheep disease virus multiplex PCR detection primer, method and application. The base sequence of the primer FMDV-F of the sheep foot-and-mouth disease virus is SEQ ID NO:1, the base sequence of the primer FMDV-R is SEQ ID NO:2; the base sequence of the primer ORFV-F of the sheep orf virus is SEQ ID NO:3, the base sequence of the primer ORFV-R is SEQ ID NO:4; the base sequence of the primer GTPV-F of the goat pox virus is SEQ ID NO:5, and the base sequence of the primer GTPV-R is SEQ ID NO:6. The application optimizes the multiplex PCR, determines the optimal reaction system and the optimal reaction procedure, and can accurately detect single or mixed infection of the sheep foot-and-mouth disease virus, the sheep orf virus and the goat pox virus, and has an important role in epidemiological investigation and epidemic disease prevention and control.

The present application belongs to the field of molecular biology detection, and particularly relates to a composition, method and use for simultaneous detection and differentiation of swine fever virus, psittacosis chlamydia, porcine reproductive and respiratory syndrome virus and porcine circovirus type II. The composition for simultaneous detection provided by the present application can realize rapid detection of four pathogen target genes in one tube of reaction solution by using multiplex fluorescence RPA detection technology, and has the advantages of high throughput, short time and low cost. On one hand, the present application can accurately detect pathogens and analyze mixed infection of pathogens, so as to achieve the effect of precise medication, reduce the use of antibiotics, save costs, and reduce the cost and increase the benefit of enterprises, and on the other hand, compared with fluorescence PCR, the detection time is greatly shortened, and the purpose of rapid detection is achieved.

The invention provides a primer probe combination, a kit and a method for synchronously detecting PCV2 and Mhp, and belongs to the technical field of animal epidemic disease molecular diagnosis. According to the technical scheme provided by the invention, the porcine circovirus type 2 and the mycoplasma hyopneumoniae can be simultaneously, rapidly and accurately detected in one reaction system, the operation steps are remarkably simplified, the detection efficiency is improved, and the detection cost is reduced. The method is high in sensitivity, and infection can be found early; the specificity is strong, the cross reaction is effectively avoided, and the result is reliable. The matched kit is convenient for standardized production and clinical popularization, provides an efficient and convenient technical tool for mixed infection monitoring of the two important pathogens in a pig farm and early diagnosis and precise prevention and control of epidemic situations, and has good application prospects and market values.

This invention relates to the field of intelligent identification technology for swine diseases, and discloses a method for identifying and making decisions about swine diseases by integrating body temperature and image features. The method separates the independent basis components of each disease in a mixed infection by performing non-negative matrix decomposition on the body temperature time spectrum. It then uses the Hungarian algorithm to establish a cross-time point correspondence between the basis components, and uses an optimal transmission algorithm to associate lesion instances with the body temperature basis components to generate a feature sequence of disease components. Finally, it uses a time-series perceptual graph neural network to perform independent inference on the symptom evolution time-series graph, outputting the disease type identification confidence and disease stage inference confidence for each pathogen. Based on a multi-pathogen joint decision rule base, it generates a coordinated treatment plan.

To provide an antiviral agent containing probiotics as an active ingredient that effectively exerts antiviral effects even in combined infections of viruses and pathogenic bacteria. [Solution] An antiviral agent is used that contains one or more strains of Lactobacillus bacteria having a 16S rRNA gene that is at least 90% identical to the nucleotide sequence shown in Sequence ID No. 1, and that has an effect of enhancing the expression of antiviral factors and / or reducing the expression of downregulatory factors of antiviral factors.

The present invention aims to provide potential drug candidates for multidrug-resistant bacterial infections. [Solution] We provide short-chain antimicrobial peptides that are rich in tryptophan and have self-assembly properties. These antimicrobial peptides consist of tryptophan (W) and a positively charged amino acid and have the ability to self-assemble. As broad-spectrum antimicrobial peptides, these antimicrobial peptides can be used to treat microbial infections, such as mixed infections caused by Pseudomonas aeruginosa and Staphylococcus aureus.

The invention provides a molecular diagnosis method for avian leukosis virus and Marek's disease virus mixed infection. The molecular diagnosis method comprises the following steps: (S01) collecting diseased material tissues; (S02) carrying out nucleic acid extraction on the sick material tissue of the sick chicken; (S03) detecting the nucleic acid by using specific primers of avian leukosis and Marek's disease; (S04) preparing a PCR (Polymerase Chain Reaction) detection system, and respectively adding ALV-env gene amplification primers and MDV-meq gene amplification primers for gene amplification; (S05) carrying out 1% agarose gel electrophoresis detection on the amplification product in the step (4), and carrying out sequence determination after determining a target band; (S06) carrying out BLAST comparison on the sequences obtained by sequencing, carrying out gene sequence analysis and drawing an evolutionary tree; and (S07) determining the virus type according to the homology and the evolutionary branches, so as to realize rapid diagnosis of ALV and MDV mixed infection, strain characteristic analysis and exclusion of other similar epidemic diseases, and provide technical support for accurate prevention and control of AL and MD in the poultry industry, provenance purification and epidemiological investigation.

The application discloses a traditional Chinese medicine feed additive for preventing and treating Simmental respiratory disease syndrome and a preparation method and application thereof, and belongs to the technical field of livestock feed additives. The additive is prepared from traditional Chinese medicine raw materials, such as ephedra, honeysuckle, forsythia, radix scrophulariae, radix houttuyniae, radix isatidis, platycodon, bitter apricot kernel, radix glycyrrhizae, radix astragali, rhizoma atractylodis, mint, loquat leaf and radix codonopsis, through decocting, extracting, low-temperature extracting after mint, concentrating and spray drying. The application adopts a three-step prevention and treatment strategy of 'dispelling lung-evil, clearing heat and resolving toxin and supporting healthy body', can effectively prevent and adjuvantly treat Simmental respiratory disease syndrome caused by mixed infection of multiple pathogens, significantly reduces the morbidity of calf BRDC, enhances the immune function of the body, promotes growth and weight gain, has the characteristics of no antibiotic residue, no drug resistance, good palatability and the like, and meets the requirements of green and healthy breeding.

A problem of the invention is to provide an antiviral agent containing as an active ingredient a probiotic which effectively exhibits an antiviral effect also on combined infection with a virus and a pathogenic bacterium. An agent containing one, two or more Lactobacillus strains which have 16S rRNA gene having an identity of at least 90% with the nucleotide sequence of SEQ ID NO: 1 and which have the action of enhancing the expression of an antiviral factor and / or the action of reducing the expression of a downregulator of an antiviral factor is used as an antiviral agent.

The invention discloses a compound immunopotentiator as well as a preparation method and application thereof, and belongs to the technical field of aquaculture. The compound immunopotentiator contains a gallnut water extract, a gallnut alcohol extract, an astragalus membranaceus water extract, an astragalus membranaceus alcohol extract, a rhodiola rosea water extract, a rhodiola rosea alcohol extract and chlorogenic acid. The compound immunopotentiator provided by the invention can significantly improve the resistance of aquaculture animals to pathogen infection, is free of environmental pollution and drug resistance risks, and has good safety and effectiveness. Experiments show that for aquatic products infected with nervous necrosis viruses, the positive rate of the nervous necrosis viruses can be obviously reduced by adding the compound immunopotentiator into feed of the aquatic products; for aquatic products treated by mixed infection of nervous necrosis virus and vibrio harveyi, the death rate of the aquatic products can be obviously reduced by adding the compound immunopotentiator into feed of the aquatic products, so that the compound immunopotentiator disclosed by the invention has a good immunoprotection effect when coping with the mixed infection of virus and bacteria.

The invention discloses application of probiotic exosomes in preparation of antibacterial products, and belongs to the technical field of biological medicines. According to the invention, the exosome secreted by lactobacillus probiotics is used for resisting bacterial and fungal mixed infection; the probiotic exosome effectively inhibits mixed infection of bacteria and fungi through various mechanisms of inhibiting adhesion of bacteria and fungi, resisting formation of a biological membrane, promoting dispersion of the biological membrane and the like. According to the invention, probiotic exosome resources with antibacterial, anti-inflammatory and mixed infection treatment functions are expanded, and a new scheme is provided for preparing products for treating related infections.

The invention discloses a primer probe group based on multiple RPA (recombinase polymerase amplification) and application of the primer probe group. The primer probe group comprises a PHV primer, a PAdv primer, a PCV primer, a Tg primer, a Cp primer and a Ca primer; wherein the nucleotide sequence of the PHV primer is as shown in SEQ ID NO. 1-2; the nucleotide sequence of the PADv primer is as shown in SEQ ID NO. 4-5; the nucleotide sequence of the PCV primer is as shown in SEQ ID NO. 7-8; the nucleotide sequence of the Tg primer is as shown in SEQ ID NO. 10 to 11; the nucleotide sequence of the Cp primer is as shown in SEQ ID NO. 13-14; the nucleotide sequence of the Ca primer is as shown in SEQ ID NO. 16-17. The kit can be used for simultaneously detecting six pigeon respiratory pathogens including PHV, PADv, PCV, Tg, Cp and Ca, is low in detection limit and high in sensitivity, can adapt to clinical mixed infection detection scenes, and avoids tedious operation of multiple independent detection.

The invention provides a primer group for identifying an African swine fever virus gene I type strain, a gene II type strain, a gene I / II type recombinant strain and mixed infection and application, and belongs to the technical field of molecular biology. The kit can accurately distinguish four conditions of a gene type I strain, a gene type II strain, a gene type I / II recombinant strain and mixed infection, the lowest detection limit is 5 copies / mu L, and the kit has high sensitivity; no cross reaction with pathogens of other pig diseases exists, and strong specificity is shown; the intra-group and inter-group repeatability variation coefficients are less than 5%, and the repeatability is good. 48 clinical sample nucleic acids identified by the national standard (GB / T 18648-2020) fluorescent PCR method are compared and detected by using the primer group, the detection result is consistent with that of the national standard method, and the sample strain type can be further identified.

The invention relates to a traditional Chinese medicine composition for preventing and treating chicken infectious rhinitis and colibacillosis mixed infection as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by mass: 40 to 80 parts of rhizoma atractylodis, 40 to 80 parts of pericarpium citri reticulatae, 40 to 80 parts of cortex magnoliae officinalis, 60 to 240 parts of radix angelicae, 60 to 120 parts of poria cocos, 60 to 120 parts of pericarpium arecae, 40 to 80 parts of raw rhizoma pinelliae (processed with ginger), 5 to 10 parts of liquorice extract, 0.4 to 0.8 part of patchouli oil and 0.2 to 0.4 part of perilla leaf oil. Especially aiming at the common pathogenesis of spleen deficiency and dampness retention in the whole process of mixed infection of infectious rhinitis and colibacillosis of laying hens, the mixed infection of infectious rhinitis and colibacillosis of laying hens is effectively prevented and treated.

The present application relates to the technical field of virus detection, in particular to a primer probe combination for identifying mixed infection pathogens of cattle and application and kit thereof. When identifying cattle pathogens, the nucleotide sequence of the upstream primer of the primer probe combination used is shown in SEQ ID No. 1, the nucleotide sequence of the downstream primer is shown in SEQ ID No. 2, the nucleotide sequence of the probe is shown in SEQ ID No. 3, and the like scheme. The present application can detect and identify bovine epidemic fever virus, bovine herpes virus 4, bovine rotavirus and clostridium welchii through four groups of primer probes. The primer probe combination provided by the present application can identify and diagnose four common pathogens of cattle through a one-tube reaction system by fluorescence quantitative PCR method, and can also identify mixed infection samples, has the advantages of accurate identification, high sensitivity, simple operation, short time consumption and the like, and can provide effective technical means for the diagnosis of cattle infectious disease pathogens and disease prevention in China.

The application provides a double-PCR differential detection method for Cryptosporidium parvum and Eimeria bovis, which is used for synchronously differentiating and detecting Cryptosporidium parvum and Eimeria bovis in a to-be-detected sample; the double-PCR differential detection method uses the following primer combination: an upstream primer Cpar-F for Cryptosporidium parvum, a downstream primer Cpar-R for Cryptosporidium parvum, an upstream primer Ebov-F for Eimeria bovis, and a downstream primer Ebov-R for Eimeria bovis. The application also provides application of the double-PCR detection method and a kit for synchronously differentiating Cryptosporidium parvum and Eimeria bovis. The double-PCR differential detection method can simultaneously and specifically amplify two target fragments in the same reaction tube, so that rapid differentiation of two pathogens and mixed infection of the two pathogens can be realized.

The application discloses an antibody, a preparation method and application thereof. The antibody has good binding activity with OXA-23 type carbapenamase, can reduce the carbapenam antibiotic resistance level of acinetobacter baumannii, thereby reducing the use concentration of meropenem in clinic, and improving the treatment effect of meropenem on acinetobacter baumannii. In addition, the antibody can also inhibit the OXA-23 protein secreted by bacteria, prevent the hydrolysis of carbapenam antibiotics by bacteria, increase the antibiotic concentration at a drug application site, and is favorable for clearing mixed infections with acinetobacter baumannii. The application provides a new strategy for treating acinetobacter baumannii infections, and has a wide application prospect.

The invention belongs to the technical field of veterinary drugs, and discloses a preparation method and application of pH response type enrofloxacin granules for treating porcine respiratory bacterial mixed infection. The invention provides the enrofloxacin coated particle, the enrofloxacin coated particle has pH response capability, is not released in a stomach pH environment and can be quickly released in a near small intestine pH environment, so that the stomach irritation of enrofloxacin is reduced, and the enrofloxacin is ensured to be absorbed at an optimal part. According to the enrofloxacin coated granules, the bitter taste of the medicine is effectively covered, the administration frequency is reduced, and the effective plasma concentration maintaining time is prolonged. Meanwhile, the enrofloxacin coated granules have a good treatment effect on porcine respiratory diseases caused by mixed infection of various bacteria.

The application belongs to the technical field of biology. More particularly, it relates to a method for accurately identifying the genotype of African swine fever virus based on high-throughput sequencing. The application selects 29 SNP sites in the B646L (P72) gene region that are highly conserved and genotype-specific, calculates the proportion of specific sites by parallel alignment with double reference sequences and base depth quantification statistics, and realizes quantitative determination of the proportion of type I, type II and mixed infection of African swine fever virus. The application breaks through the limitation that the traditional method cannot determine the mixed infection of African swine fever virus, realizes the objective determination of mixed strains, and provides a reliable tool for virus identification under complex infection scenarios. The application is completed based on high-throughput sequencing data, the analysis process is standardized, the subjective error caused by manual judgment is reduced, the consistency and repeatability of the results are strong; at the same time, the output statistical table and the results are intuitive and traceable, and have good popularization and application value.

This invention relates to a triple nano-PCR detection method for silkworm microsporidia, Beauveria bassiana, and nucleopolyhedrovirus, belonging to the field of molecular diagnostic technology. Addressing the technical problem of existing silkworm disease diagnostic methods' inability to simultaneously, efficiently, and sensitively identify these three commonly mixed infection pathogens, this invention provides an integrated solution. The key technical points include extracting DNA from the sample to be tested, using this DNA as a template, performing nano-PCR amplification in the same reaction system using a specific primer set, and subsequently detecting the amplification products by agarose gel electrophoresis. The infection status of silkworm microsporidia, Beauveria bassiana, and nucleopolyhedrovirus is determined based on the specific bands at 733 bp, 230 bp, and 860 bp, respectively. This method is mainly used for the rapid, simultaneous diagnosis and identification of mixed infections of microsporidia, Beauveria bassiana, and hemorrhagic septicemia during silkworm rearing.

The present invention relates to the use of polyhomoarginine comprising 6-26 homoarginine residues or a pharmaceutically acceptable salt thereof, in the treatment of an infection, wherein the infection is a fungal infection, a bacterial infection or a combination thereof. The present invention also relates to the use of polyhomoarginine comprising 6-26 homoarginine residues or a pharmaceutically acceptable salt thereof in a formulation or in a contact lens.