The present application belongs to the technical field of
plant species identification, and particularly relates to a detection and identification kit, method and application of
Aglaia odorata. The kit contains an upstream primer with a
nucleotide sequence of SEQ ID NO. 1, a downstream primer with a
nucleotide sequence of SEQ ID NO. 2, and a
restriction enzyme Sma I. The primer is used for PCR amplification of sample
DNA, and the amplification product is digested by a
restriction enzyme Sma I. The
enzyme-digested product is subjected to
agarose gel electrophoresis, and according to the number and size of specific electrophoretic bands,
Aglaia odorata and its similar species can be distinguished and identified conveniently and quickly. The method has the advantages of intuitive results, high specificity, high identification efficiency, and avoidance of false positive amplification.