The application discloses a preparation method of a specific
yolk antibody resisting
mycoplasma synoviae diseases. The preparation method comprises the following steps of recovering
mycoplasma synoviae strains, performing propagation culture for
three generations, performing sufficient inactivation, performing centrifuging, and taking precipitate so as to obtain a
mycoplasma synoviae
antigen; performing mixed emulsification on a
mycoplasma synoviae antigen solution and a
white oil adjuvant, performing basic immunization on each hen, and performing injection for 0.5ml; and after the basic immunization, performing strengthened immunization twice, wherein an immunization method and the dosage are the same as those of the basic immunization, after the first-time strengthened immunization, beginning to collect immunized eggs, disinfecting the obtained immunized eggs with
alcohol, breaking shells, collecting
yolk, and performing purifying to obtain the specific
yolk antibody. The specific yolk
antibody resisting
mycoplasma synoviae diseases, prepared by the preparation method can effectively prevent and treat the
mycoplasma synoviae diseases. The specific yolk antibody is easy in
mass production and low in cost, and an obtained product is a safe preparation resisting the mycoplasma synoviae diseases.