4 results about "Mycoplasma synoviae" patented technology

This invention provides a composite adjuvant, its preparation method, and its application in vaccine preparation, belonging to the field of vaccine adjuvant technology. The composite adjuvant of this invention has low viscosity, only 12 mPa·s at 20°C, ensuring smooth injection; it exhibits high biocompatibility, with no deaths or organ abnormalities in acute toxicity experiments in mice, and no local or systemic adverse reactions or stress responses in chickens after injection; it also demonstrates excellent immunogenicity, achieving a 90% seroconversion rate for Mycoplasma cambogia and an 80% seroconversion rate for Mycoplasma synoviae 28 days after immunization with an inactivated Mycoplasma synoviae vaccine.

The application provides a mycoplasma gallisepticum triple subunit vaccine and application thereof, and the vaccine comprises EF-TU protein with an amino acid sequence as shown in SEQ ID NO. 1, PdHD protein with an amino acid sequence as shown in SEQ ID NO. 2, MS1360 protein with an amino acid sequence as shown in SEQ ID NO. 3 and a pharmaceutically acceptable adjuvant. The application realizes antigen epitope complementation and immune synergistic enhancement by screening the optimal vaccine formula through two-by-two mixed combination and three-protein concentration gradient mixed system screening of EF-TU, PdHD and MS1360 three high immunogenic membrane proteins, and solves the problems of low protection rate and unstable immune effect of the existing vaccine.

The application belongs to the technical field of microorganism detection, and particularly relates to a primer probe combination, a kit and application for detecting chicken synovial capsule mycoplasma based on RAA-LFD. The nucleotide sequence of the upstream primer in the primer probe combination is shown in SEQ ID NO. 3, the nucleotide sequence of the downstream primer is shown in SEQ ID NO. 4, and the nucleotide sequence of the probe is shown in SEQ ID NO. 10. Based on the primer probe combination, the application further develops a kit and a method for detecting chicken synovial capsule mycoplasma. The method has high detection accuracy, good specificity and high sensitivity, can rapidly and accurately realize on-site rapid visual detection of chicken synovial capsule mycoplasma, and is particularly suitable for use in the field.

This invention relates to the field of identification technology for Mycoplasma synoviae MS-H strain and wild-type strain, specifically a LAMP primer-probe composition and kit for identifying Mycoplasma synoviae MS-H strain and wild-type strain. It includes an upstream outer primer with the nucleotide sequence shown in SEQ NO:1, a downstream outer primer with the nucleotide sequence shown in SEQ NO:2, an upstream inner primer with the nucleotide sequence shown in SEQ NO:3, a downstream inner primer with the nucleotide sequence shown in SEQ NO:4, an MS-H strain probe with the nucleotide sequence shown in SEQ NO:5, and an MS wild-type strain probe with the nucleotide sequence shown in SEQ NO:6. This invention uses MGB to enhance the thermostability of the probe and combines RNase H2-mediated blocking probe specific activation technology with an enhancer to improve the specificity of the reaction system, enabling LAMP to recognize single-base differences at isothermal conditions, with a detection limit reaching 1 copy / µL.