40 results about "Orf virus" patented technology

The invention provides a detection method for detecting the orf virus of sheep. Real-time fluorescent quantitative PCR (polymerase chain reaction) detection is performed on a DNA (deoxyribonucleic acid) template of a sample by designing a pair of primers and a probe. The invention further provides a kit for detecting the orf virus of the sheep by utilizing the detection method. The real-time quantitative method which is established against H2L genes in a conserved region of the ORFV (orf virus) is high in specificity and good in stability. The kit for detecting the orf virus of the sheep, provided by the invention, is simple and convenient to operate, a user only needs to add DNA of the sample to be detected into a reaction tube, detection results can be obtained according to an amplification curve, the operation is time-saving and labor-saving, the cost is lower, and the time is shortened from original 3-4 hours to 1-2 hours; and furthermore, the sensitivity is higher than that of the ordinary PCR, and the detection method and the kit can be used for detecting the low content of the orf virus of the sheep, can be suitable for all levels of prevention and control units, basic-level veterinary stations, large and medium-sized breeding farms and the like in China, and has better application prospects.