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Aphthovirus (from the Greek aphtha-, vesicles in the mouth) is a viral genus of the family Picornaviridae. Aphthoviruses infect vertebrates, and include the causative agent of foot-and-mouth disease. Foot-and-mouth disease virus (FMDV) is the prototypic member of the genus Aphthovirus. There are seven FMDV serotypes: A, O, C, SAT 1, SAT 2, SAT 3 and Asia 1, and four non-FMDV serotypes belonging to three additional species Bovine rhinitis A virus (BRAV), Bovine rhinitis B virus (BRBV) and Equine rhinitis A virus (ERAV).
Equine rhinovirus 1 proteins
A determination of the nucleotide sequence for the genome of Equine rhinovirus 1 (ERhV1), a horse-respiratory pathogen of heretofor uncertain taxonomic status, reveals a structure and predicted amino acid sequence that are more similar to those of foot-and-mouth disease viruses, the only members of the aphthovirus genus, than of other picornaviruses. These insights inform an understanding of ERhV1 virion proteins and virus-like particles, as well as the design of probes, primers, antigens, vectors, diagnostics and tests of relevance to ERhV1.
Owner:UNIVERSITY OF MELBOURNE
Sheep aphthovirus Asial type multi-epitope recombinant vaccine and preparation method thereof
ActiveCN101864434AComprehensive immune efficiencyComprehensive evaluation of immune efficiencyGenetic material ingredientsVirus peptidesAdjuvantAdditive ingredient
The invention discloses a sheep aphthovirus Asial type multi-epitope recombinant vaccine and a preparation method thereof. The recombinant vaccine comprises the following ingredients: a protein encoded by aphthovirus multi-epitope genes and carrier protein fusion genes, and an aphthovirus 3D protein. The preparation method comprises the following steps: respectively diluting the protein encoded by the aphthovirus multi-epitope genes and the carrier protein fusion genes and the aphthovirus 3D protein; then, uniformly mixing the diluted proteins; adding auxiliary agents into the mixture; carrying out emulsification; and obtaining the sheep aphthovirus Asia1 type multi-epitope recombinant vaccine. Animal model and animal immune efficiency experiments show that the sheep Asial epitope recombinant vaccine of the invention can generate overall immunoprotection reaction, injected immune sheep can be induced to generate high-level neutralizing antibodies, and can also induce the cellullar immunologic response. The recombinant vaccine of the invention can effectively protect animals from the virulent strain attack of the aphthovirus.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Adenoviral vector-based foot-and-mouth disease vaccine
ActiveUS8323663B2SsRNA viruses positive-senseViral antigen ingredientsAntigenNucleic acid sequencing
The invention is directed to an adenoviral vector comprising at least one nucleic acid sequence encoding an aphthovirus antigen and / or a cytokine operably linked to a promoter. The adenoviral vector is replication-deficient and requires at most complementation of both the E1 region and the E4 region of the adenoviral genome for propagation. The invention also is directed to a method of inducing an immune response in a mammal comprising administering to the mammal a composition comprising the aforementioned adenoviral vector.
Owner:US SEC AGRI +2
Goatpox virus-orf virus combined cell attenuated vaccine and its preparation method and use
ActiveCN104758928AGuaranteed immune protectionSave solutionAntiviralsMacromolecular non-active ingredientsActive componentAttenuated vaccine
The invention discloses a goatpox virus-orf virus combined cell attenuated vaccine and its preparation method and use. The goatpox virus-orf virus combined cell attenuated vaccine contains an active component which comprises goatpox and contagious pustular dermatitis virus cell passage-attenuated viruses or passaged viruses separated by the inventor and subjected to passage attenuation culture, wherein the goatpox virus cell passage-attenuated virus is a Goatpox Virus HN-XY2010F43 strain and has a preservation number of CCTCC NO: V201503 and the contagious pustular dermatitis virus cell passage-attenuated virus is an Orf Virus HB-TS09F65 strain and has a preservation number of CCTCC NO: V201406. A toxin counteracting protection test result shows that the goatpox virus-orf virus combined cell attenuated vaccine is safe and can produce an effective immunization protection function. The goatpox virus-orf virus combined cell attenuated vaccine and its preparation method have important practical meanings for development of safe and efficient attenuated vaccines and large-scale production of the goatpox virus-orf virus combined cell attenuated vaccine for preventing and controlling goatpox and orf from the disclosed attenuated strain.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
RT-RPA detection reagent kit for quickly detecting pest des petits ruminant viruses and application thereof
ActiveCN105567872AShorten test timeLow reaction temperatureMicrobiological testing/measurementPustular dermatitisOvis
The invention discloses an RT-RPA detection reagent kit for quickly detecting pest des petits ruminant viruses and application thereof. The reagent kit comprises a pair of primers and a probe. The sequences of the primers are as shown in SEQ ID NO.1 and SEQ ID NO.2, and the sequence of the probe is as shown in SEQ ID NO.3. Experiments prove that the reagent kit can specifically detect the pest des petits ruminant viruses and does not amplify sheep pox viruses, goat pox viruses, contagious pustular dermatitis sheep viruses, foot and mouth disease viruses and other important sheep infection viruses under the same condition, and it is illustrated that the method has good specificity. Sensitivity experiments show that the RT-RPA primers and the probe for quickly detecting the pest des petits ruminant viruses can at least detect 150 copied templates at the temperature of 40 DEG C and under the condition that amplification is conducted for 20 min and have high conformity with RT-qPCR. Accordingly, the reagent kit can quickly, efficiently and sensitively detect the pest des petits ruminant viruses, and an effective technical means is provided for identification and diagnosis of the pest des petits ruminant viruses.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Preparation and use of swine foot-and-mouth disease recombinant immune composite peptide
InactiveCN101376887AImproving immunogenicityGood immune effectBacteriaMicroorganism based processesEscherichia coliAdjuvant
The invention relates to a method for constructing porcine fmd recombinant immune complex peptide and an application thereof, which belongs to the technical field of biological engineering. The immune multi-peptide adjuvant is fused with aphthovirus novel auxiliary T cell epiposition (I-S-I-S-E-I-K-G-V-I-V-H-K-I-E-T-I-L-F) and Bursin mimic peptide (T-P-N-L-K-H-G) by a flexible Linker. A fusion gene is inserted into an expression carrier to be converted into colon bacillus, gene engineering strains which express the porcine fmd recombinant immune complex peptide with high efficiency are obtained, and the fusion protein of the porcine fmd recombinant immune complex peptide is obtained after liquid culture and purification; the thioredoxin in the fusion protein is removed by enterokinase with His label on the N end, and the single porcine fmd recombinant immune complex peptide can be obtained after affinity chromatography purification with very high sample purity. The recombinant immune complex peptide can serve as the novel immune multi-peptide adjuvant of the foot-and-mouth disease vaccine. Animal immune experiments prove that the method has high safety but no toxicity and side effect, and can effectively enhance the immunity of organisms and cells and the immunity level of body fluid.
Owner:NANJING AGRICULTURAL UNIVERSITY
Real-time fluorescent quantitative PCR (polymerase chain reaction) detecting reagent kit for orf virus
InactiveCN102676694AControl spreadQuality assuranceMicrobiological testing/measurementMicroorganism based processesNucleotideNucleotide sequencing
The invention discloses a real-time fluorescent quantitative PCR (polymerase chain reaction) detecting reagent kit for an orf virus and belongs to the technical field of viral molecular biology detection. The real-time fluorescent quantitative PCR detecting reagent kit for the orf virus comprises a pair of primers of nucleotide sequences shown in SEQ ID NO:1-2 and a TaqMan probe of a nucleotide sequence shown in SEQ ID NO:3. The detecting reagent kit can quickly and accurately detect the orf virus from samples and is high in detecting sensitivity and simple and convenient to sample, detecting efficiency is greatly improved, pollution can be effectively prevented, and the detecting reagent kit is of practical significance in the field of inspection and quarantine of import and export animals.
Owner:GUANGZHOU BONIZZI BIOTECH CO LTD
Selection marker-free recombinant goat-pox virus for expression of Orf virus F1L protein and construction method thereof
InactiveCN110724674AHigh expressionContinuous and efficient expressionViral antigen ingredientsMicroorganism based processesEngineeringRecombinant virus vaccine
Through homologous recombination and by innovative utilization of plaque and reverse plaque screening technologies, the selection marker-free recombinant virus, especially the rGTPV-F1L recombinant goat-pox virus is finally obtained. The method is simple and effective, is easy to operate, provides the selection marker-free recombinant virus for the development of a recombinant virus vaccine, provides new ideas and measures for the researches on a novel ORF vaccine, and has broad application prospects.
Owner:CHONGQING ACAD OF ANIMAL SCI
Equine rhinovirus 1 proteins
Equine rhinovirus 1 (ERhV1) is a respiratory pathogen of horses which has an uncertain taxonomic status. The nucleotide sequence of the ERhV1 genome and amino acid sequence have been substantially determined (FIG. 2). The predicted polyprotein was encoded by 6,741 nucleotides and possessed a typical picornavirus proteolytic cleavage pattern, including a leader polypeptide. The genomic structure and predicted amino acid sequence of ERhV1 were more similar to those of foot-and-mouth disease viruses (FMDV), the only members of the aphthovirus genus, than other picornaviruses. Nucleotide sequences coding for the complete polyprotein, the polymerase, and VP1 were analyzed separately. The phylogenetic trees confirmed that ERhV1 was more closely related to aphthoviruses than to other picornaviruses. Virion proteins and virus-like particles are described and probes, primers, antigens, vectors, diagnostics and tests developed.
Owner:UNIVERSITY OF MELBOURNE
Recombinant goatpox virus capable of carrying out coexpression on orf virus B2L and F1L protein
The invention provides a recombination transfer vector used for constructing a recombinant goatpox virus. The transfer vector mainly comprises the left arm and the right arm of a TK gene, two reversep7.5K promoters, an orf virus B2L gene and F1L gene independently regulated and controlled by the two reverse p7.5K promoters, a p11K promoter, an enhanced green fluorescence protein (eGFP) gene and two forward Loxp sequences, wherein the transfer vector and the goatpox virus generate homologous recombination in goat testicular cells so as to obtain the recombinant goatpox virus capable of expressing the eGFP, under the function of Cre enzyme, the eGFP gene and the p11K prompter are specifically knocked out, and therefore, the potential recombinant goatpox virus capable of carrying out the coexpression on the orf virus B2L and F1L protein is obtained.
Owner:CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENT
Sheep pox and aphtha bigeminy cell attenuated vaccine and preparation method and application thereof
ActiveCN106139141AAvoid the risk of carrying other pathogensQuality improvementPowder deliveryViral antigen ingredientsAttenuated vaccineSheeppox
The invention discloses a sheep pox and aphtha bigeminy cell attenuated vaccine and a preparation method and application thereof. The effective ingredient of the sheep pox and aphtha bigeminy cell attenuated vaccine is sheep pox virus and contagious pustular dermatitis virus cell passage attenuate virus or passage virus thereof which are separated through the inventor and obtained after passage attenuate culture, wherein the sheep pox virus cell passage attenuate virus is Sheeppox Virus GS-WW 2010 F44 strain, the preservation number of CCTCC NO: V201504, the sheep pox virus (contagious pustular dermatitis virus) cell passage attenuate virus is Orf Virus HB-TS09F65 strain, and the preservation number is CCTCC NO:V201406. Toxin attacking protection test results show that the sheep pox and aphtha bigeminy cell attenuated vaccine is safe and capable of producing effective immune protection force. Accordingly, the sheep pox and aphtha bigeminy cell attenuated vaccine is of an important practical significance in researching safe and efficient attenuated vaccine and preventing and controlling sheep pox and aphtha bigeminy.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Adenoviral vector-based foot-and-mouth disease vaccine
The invention is directed to an adenoviral vector comprising at least one nucleic acid sequence encoding an aphthovirus antigen and / or a cytokine operably linked to a promoter. The adenoviral vector is replication-deficient and requires at most complementation of both the E1 region and the E4 region of the adenoviral genome for propagation. The invention also is directed to a method of inducing an immune response in a mammal comprising administering to the mammal a composition comprising the aforementioned adenoviral vector.
Owner:US SEC AGRI +2
A multiplex PCR kit for the simultaneous detection of six viruses in sheep and goats
InactiveCN105331742BStrong specificityHigh sensitivityMicrobiological testing/measurementMultiplexNucleotide
A multiplex-PCR (polymerase chain reaction) kit for detecting six viruses of sheep and goats simultaneously comprises six pairs of specific primers, wherein the primers used for detection of the bluetongue virus are BTV-F and BTV-R respectively; the primers for detection of the foot and mouth disease virus are FMDV-F and FMDV-R respectively; the primers for detection of the peste des petits ruminants virus are PPRV-F and PPRV-R respectively; the primers for detection of the sheep pox virus are SPPV-F and SPPV-R respectively; the primers for detection of the goat pox virus are GTPV-F and GTPV-R respectively; the primers for detection of the sore mouth disease virus are ORFV-F and ORFV-R respectively. The sequences of the primers are nucleotide sequences shown in SEQ ID NO: 1-12. The multiplex-PCR kit can be used for detecting nucleic acid of the six viruses including the bluetongue virus, the foot and mouth disease virus, the peste des petits ruminants virus, the sheep pox virus, the goat pox virus and the sore mouth disease virus in sheep and goat clinical samples and has the characteristics of high sensitivity, high specificity, simple operation and reduction of detection time.
Owner:NORTHWEST A & F UNIV
A kind of rt-rpa detection kit for rapid detection of Peste des petits ruminants virus and its application
ActiveCN105567872BShorten test timeLow reaction temperatureMicrobiological testing/measurementPustular dermatitisFoot mouth disease virus
The invention discloses an RT-RPA detection reagent kit for quickly detecting pest des petits ruminant viruses and application thereof. The reagent kit comprises a pair of primers and a probe. The sequences of the primers are as shown in SEQ ID NO.1 and SEQ ID NO.2, and the sequence of the probe is as shown in SEQ ID NO.3. Experiments prove that the reagent kit can specifically detect the pest des petits ruminant viruses and does not amplify sheep pox viruses, goat pox viruses, contagious pustular dermatitis sheep viruses, foot and mouth disease viruses and other important sheep infection viruses under the same condition, and it is illustrated that the method has good specificity. Sensitivity experiments show that the RT-RPA primers and the probe for quickly detecting the pest des petits ruminant viruses can at least detect 150 copied templates at the temperature of 40 DEG C and under the condition that amplification is conducted for 20 min and have high conformity with RT-qPCR. Accordingly, the reagent kit can quickly, efficiently and sensitively detect the pest des petits ruminant viruses, and an effective technical means is provided for identification and diagnosis of the pest des petits ruminant viruses.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Application of sheep-derived LGALS1 in inhibition of orf virus
The invention discloses application of sheep-derived LGALS1 in inhibition of orf virus, and belongs to the field of genetic engineering. Research finds that the proliferation of OrfV can be inhibited by overexpressing LGALS1 gene in LT cells; the proliferation of OrfV can be promoted by interference expression of the LGALS1 gene. Therefore, the LGALS1 gene or a biological material containing the LGALS1 gene or the LGALS1 protein can be used for preparing a product for resisting the orf virus.
Owner:GUIZHOU UNIV
Ophtha virus expressing CXCL9 and application thereof in preparation of antitumor drugs
The invention provides a novel recombinant orf virus (ORFV), the recombinant orf virus expresses a chemotactic factor CXC ligand 9, the recombinant orf virus is a strain ORFV [delta] 005-008 (expressed by ORFV [delta]) with ORFV ORFs 005-008 gene deletion, and the Cxc19 gene expressed by the recombinant orf virus is one of a mouse source, a human source and a cat source. The ORFV oncolytic virus is used as a carrier to develop a tumor immunotherapy drug ORFVdelta-Cxc19 expressing CXCL9, the CXCL9 is expressed in tumor cells by using the characteristics of virus infection, the collection of T cells in a tumor microenvironment is promoted while the tumor cells are cracked, and the anti-tumor effect of the oncolytic virus is enhanced. The invention provides a new strategy for tumor treatment.
Owner:JILIN UNIVERSITY
A dual-cell attenuated vaccine for sheep pox and aphthous ulcers and its preparation method and application
ActiveCN106139141BGuaranteed immune protectionSave solutionPowder deliveryViral antigen ingredientsSheeppoxTGE VACCINE
The invention discloses a sheep pox and aphtha bigeminy cell attenuated vaccine and a preparation method and application thereof. The effective ingredient of the sheep pox and aphtha bigeminy cell attenuated vaccine is sheep pox virus and contagious pustular dermatitis virus cell passage attenuate virus or passage virus thereof which are separated through the inventor and obtained after passage attenuate culture, wherein the sheep pox virus cell passage attenuate virus is Sheeppox Virus GS-WW 2010 F44 strain, the preservation number of CCTCC NO: V201504, the sheep pox virus (contagious pustular dermatitis virus) cell passage attenuate virus is Orf Virus HB-TS09F65 strain, and the preservation number is CCTCC NO:V201406. Toxin attacking protection test results show that the sheep pox and aphtha bigeminy cell attenuated vaccine is safe and capable of producing effective immune protection force. Accordingly, the sheep pox and aphtha bigeminy cell attenuated vaccine is of an important practical significance in researching safe and efficient attenuated vaccine and preventing and controlling sheep pox and aphtha bigeminy.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
A kind of rapid separation method of aphthous ulcer virus
ActiveCN104694484BGuaranteed freshShorten the separation cycleMicroorganism based processesViruses/bacteriophagesDiseaseTesticle
The invention relates to a method for rapidly separating a sore mouth disease virus and in particular relates to a method for separating the sore mouth disease virus from peripheral blood of ill sheep and recessive virus-carried sheep by utilizing bovine testicle cells. The sore mouth disease virus is separated from the peripheral blood of ill sheep suffering from the sore mouth disease and the recessive virus-carried sheep by utilizing the bovine testicle cells, a convenient and rapid method is provided for separating the sore mouth disease, and convenience is brought to production and scientific research. The method for rapidly separating sore mouth disease virus disclosed by the invention comprises the following steps: (1) collecting and detecting a sore mouth disease virus sample; (2) treating the sample; and (3) separating the sore mouth disease virus.
Owner:NORTHWEST A & F UNIV
Sheep disease virus multiplex PCR detection primer, method and application
The application belongs to the technical field of virus detection, and discloses a sheep disease virus multiplex PCR detection primer, method and application. The base sequence of the primer FMDV-F of the sheep foot-and-mouth disease virus is SEQ ID NO:1, the base sequence of the primer FMDV-R is SEQ ID NO:2; the base sequence of the primer ORFV-F of the sheep orf virus is SEQ ID NO:3, the base sequence of the primer ORFV-R is SEQ ID NO:4; the base sequence of the primer GTPV-F of the goat pox virus is SEQ ID NO:5, and the base sequence of the primer GTPV-R is SEQ ID NO:6. The application optimizes the multiplex PCR, determines the optimal reaction system and the optimal reaction procedure, and can accurately detect single or mixed infection of the sheep foot-and-mouth disease virus, the sheep orf virus and the goat pox virus, and has an important role in epidemiological investigation and epidemic disease prevention and control.
Owner:GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
Preparation method of permanent cell line for multiplying orf virus
ActiveCN102533660BStable proliferationMicroorganism based processesForeign genetic material cellsAttenuated vaccineAphthovirus
The invention relates to a preparation method of a permanent cell line for multiplying an orf virus. The aim that the virus can be stably multiplied in a large amount is achieved by inoculating a calf testicular cell line to the orf virus and a stable cell environment and a standardized culture system for developing and producing an attenuated vaccine of the orf virus are provided. The preparation method comprises the following steps of: firstly, acquiring, separating and culturing a calf testicular cell; secondly, permanently establishing the calf testicular cell; and thirdly, carrying out multiplication culture on the orf virus in the cell line.
Owner:NORTHWEST A & F UNIV
Orf virus attenuated strain and application thereof
InactiveCN111139226AHigh-quality material basePremium Choice SpaceViral antigen ingredientsInactivation/attenuationTGE VACCINEAphthovirus
The invention provides an orf virus attenuated strain and an application thereof. The attenuated strain is an orf virus attenuated strain QFnm2015, the preservation institution is the China Center forType Culture Collection, and the preservation number is CCTCC NO: V201903. According to the invention, the isolated orf virus is proliferated and cultured on lamb testicular cells, and the orf virusattenuated strain is cultured through continuous passage. The isolated strain of the orf virus provides a high-quality material basis and selection space for developing an effective vaccine of endemicORFV, and has important significance for prevention and treatment of orf.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY
Recombinant DNA vaccine for resisting ecthyma and sheep pox and recombinant plasmid thereof
The invention provides a recombinant DNA vaccine and a recombinant plasmid thereof, the DNA vaccine recombinant plasmid comprises an antigen coding sequence and a plasmid vector, the antigen coding sequence comprises an orf antigen coding sequence and a sheep pox antigen coding sequence which are connected in series, and an orf antigen and a sheep pox antigen are expressed as independent antigen proteins. The invention provides construction of a double-gene recombinant DNA (Deoxyribose Nucleic Acid) vaccine of an orf virus gene and a sheep pox virus gene and analysis of a mouse immune effect. According to the invention, an orf virus B2L (011) gene and a sheep pox virus P32 gene are selected, the two genes are connected in series by using a self-cleaving peptide P2A, and the self-cleaving peptide P2A generates self-cleaving in eukaryotic cells, so that two antigen proteins are independently expressed on an eukaryotic expression vector pcDNA3.1 (+). The recombinant DNA vaccine prepared by the invention also has a better immune effect after being used for immunizing a mouse, and can be used as a reference of a candidate vaccine.
Owner:JILIN UNIV
Preparation and use of swine foot-and-mouth disease recombinant immune composite peptide
InactiveCN101376887BImprove immunityImproving immunogenicityBacteriaMicroorganism based processesEscherichia coliSide effect
The invention relates to a method for constructing porcine fmd recombinant immune complex peptide and an application thereof, which belongs to the technical field of biological engineering. The immune multi-peptide adjuvant is fused with aphthovirus novel auxiliary T cell epiposition (I-S-I-S-E-I-K-G-V-I-V-H-K-I-E-T-I-L-F) and Bursin mimic peptide (T-P-N-L-K-H-G) by a flexible Linker. A fusion gene is inserted into an expression carrier to be converted into colon bacillus, gene engineering strains which express the porcine fmd recombinant immune complex peptide with high efficiency are obtained, and the fusion protein of the porcine fmd recombinant immune complex peptide is obtained after liquid culture and purification; the thioredoxin in the fusion protein is removed by enterokinase with His label on the N end, and the single porcine fmd recombinant immune complex peptide can be obtained after affinity chromatography purification with very high sample purity. The recombinant immune complex peptide can serve as the novel immune multi-peptide adjuvant of the foot-and-mouth disease vaccine. Animal immune experiments prove that the method has high safety but no toxicity and side effect, and can effectively enhance the immunity of organisms and cells and the immunity level of body fluid.
Owner:NANJING AGRICULTURAL UNIVERSITY
A kind of permanent cell line for the multiplication of sheep oral ulcer virus and its establishment method
ActiveCN107287149BReduce usageCell dissociation methodsViral antigen ingredientsVirus multiplicationAttenuated vaccine
The invention relates to a permanent cell line for ecthyreosis virus multiplication. The name of the cell line is a goat testis cell GT26, the cell line is preserved at the China Center for Type Culture Collection on March 1, 2017, and the preservation number is CCTCC NO:C201734. An establishment method of the permanent cell line comprises the steps of (1) permanent establishment of the goat testis cell; (2) low-serum domestication of the goat testis cell; and (3) cultivation of an ecthyreosis virus in a newborn goat testis cell line. The ecthyreosis virus is grafted by using the goat testis cell line, thereby achieving the target of stable virus multiplication and providing a stable cell environment and a standardized culture method for development and production of an attenuated vaccine and an inactivated vaccine of the ecthyreosis virus.
Owner:陕西博德越生物科技有限公司
A colloidal gold test strip for detecting sheep pox virus antibodies, its preparation method and application
This invention relates to a colloidal gold test strip for detecting sheep pox virus antibodies, its preparation method, and its application. First, this invention prepares recombinant 122 protein, which exhibits significant antigenicity and immunogenicity, making it a good target for diagnosing sheep pox virus. Second, this invention prepares a colloidal gold test strip for detecting sheep pox virus antibodies. The test strip has a PVC base plate with a sample pad, a gold-labeled pad, a nitrocellulose membrane, and an absorbent pad arranged sequentially. The gold-labeled pad is coated with gold-labeled recombinant 122 protein. The test strip of this invention can simultaneously detect antibodies against sheep pox virus, bovine nodular dermatosis virus, and goat pox virus, with sensitivities of 1:128, 1:128, and 1:64, respectively. It shows no cross-reactivity with bovine viral diarrhea virus, foot-and-mouth disease virus type O, foot-and-mouth disease virus type A, sheep stomatitis virus, brucellosis, and Escherichia coli, exhibiting good specificity and a diagnostic concordance rate of 93%.
Owner:LANZHOU VETERINARY RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES(LANZHOU BRANCH CENTER OF CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENTER)
A recombinant orf virus expressing porcine circovirus type 3 cap protein and a preparation method and application thereof
The application discloses a recombinant orf virus for expressing PCV3 Cap protein, and the recombinant orf virus is obtained by the following steps: firstly, replacing the 132 gene of the orf virus with a nucleotide sequence, such as the gene sequence 1 of SEQ ID NO: 5, to obtain a recombinant virus 1; and secondly, replacing the nucleotide sequence, such as the gene sequence 1 of SEQ ID NO: 5, in the recombinant virus 1 with a nucleotide sequence, such as the gene sequence 2 of SEQ ID NO: 6, to obtain the recombinant orf virus. The application replaces the 132 gene of the ORFV virus with the optimized Cap gene of the PCV3 to prepare the recombinant orf virus. The recombinant orf virus can stably express the PCV3 Cap protein, and provides a vaccine candidate strain for preventing the PCV3 related diseases and a feasible technical platform for developing the recombinant orf virus of other viruses.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY
Kit of double RPA detection of aphthovirus and vesicular stomatitis virus
ActiveCN112899400ARealize double RPA detectionLower requirementMicrobiological testing/measurementMicroorganism based processesNucleotideFoot mouth disease virus
The invention discloses a kit of double RPA detection of aphthovirus and vesicular stomatitis virus. The kit includes an aphthovirus RPA detection primer set consisting of nucleotide sequences shown as SEQ. ID. NO. 1 and SEQ. ID. NO. 6 and a vesicular stomatitis virus RPA detection primer set consisting of nucleotide sequences shown as SEQ. ID. NO. 8 and SEQ. ID. NO. 11. Based on a recombinase polymerase amplification method, the kit takes the cDNA formed by RNA reverse transcription as a template and performs amplification reaction under optimized reaction temperature and time, and detection results can be obtained through nucleic acid gel electrophoresis. The kit can be used to simultaneous detect aphthovirus and vesicular stomatitis virus, is simple in operation and has high sensibility and specificity, therefore rapid detection kit can be provided for on-site screening of pathogens.
Owner:NORTHWEST A & F UNIV
Yeast cDNA library and construction method of a sheep testis fibroblast membrane system
ActiveCN108998443BOvercome limitationsLibrary creationProtein nucleotide librariesCDNA libraryCell membrane
The invention provides a method for preparing a yeast cDNA library of sheep testis fibroblast membrane system, which comprises preparing sheep testis fibroblasts; extracting RNA from sheep testis fibroblasts; synthesizing cDNA and homogenizing the cDNA library; cDNA and pPR3-N carrier Ligation, transformation of the ligation product into host cell DH5α, obtaining the yeast cDNA library of the sheep testis fibroblast membrane system, and performing cloning verification; screening the yeast cDNA library of the sheep testis fibroblast membrane system. The cDNA library capacity of the present invention is 7.5 × 10 5 , the size of the fragments is 200‑2000bp, the average length is 1000bp, and the recombination rate of the library is 100%. The library was screened by using the membrane protein of aphthus virus, which proved that the library was a high-quality library.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
ORF130 gene deleted strain of orf virus as well as preparation method and application of ORF130 gene deleted strain
The invention belongs to the field of preparation of veterinary vaccines, and particularly relates to an orf virus ORF130 gene deleted vaccine strain as well as a preparation method and application thereof, and the gene deleted vaccine strain is prepared by inhibiting ORF130 gene expression in orf virus and deleting or conditionally knocking out an ORF130 gene. The highest TCID50 of the obtained gene deletion strain ORFV-HB-TS09F65 ORF130 can reach 10 <-5.12 > / 0.1 mL, and the toxicity of the gene deletion strain ORFV-HB-TS09F65 ORF130 is obviously weakened; compared with an artificial passage attenuated orf vaccine strain, the orf vaccine strain has no difference in safety; meanwhile, in the vaccine immunization process, the specific marker in the region can be used as an effective target for distinguishing vaccine immunization from wild virus infection, so that the gene deletion strain is suitable for being used as a candidate strain of the orf gene deletion vaccine.
Owner:LANZHOU VETERINARY RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES(LANZHOU BRANCH CENTER OF CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENTER)
Sheep aphthovirus Asial type multi-epitope recombinant vaccine and preparation method thereof
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI