30 results about "Aphthovirus" patented technology

The invention discloses an RT-RPA detection reagent kit for quickly detecting pest des petits ruminant viruses and application thereof. The reagent kit comprises a pair of primers and a probe. The sequences of the primers are as shown in SEQ ID NO.1 and SEQ ID NO.2, and the sequence of the probe is as shown in SEQ ID NO.3. Experiments prove that the reagent kit can specifically detect the pest des petits ruminant viruses and does not amplify sheep pox viruses, goat pox viruses, contagious pustular dermatitis sheep viruses, foot and mouth disease viruses and other important sheep infection viruses under the same condition, and it is illustrated that the method has good specificity. Sensitivity experiments show that the RT-RPA primers and the probe for quickly detecting the pest des petits ruminant viruses can at least detect 150 copied templates at the temperature of 40 DEG C and under the condition that amplification is conducted for 20 min and have high conformity with RT-qPCR. Accordingly, the reagent kit can quickly, efficiently and sensitively detect the pest des petits ruminant viruses, and an effective technical means is provided for identification and diagnosis of the pest des petits ruminant viruses.

The invention discloses a sheep pox and aphtha bigeminy cell attenuated vaccine and a preparation method and application thereof. The effective ingredient of the sheep pox and aphtha bigeminy cell attenuated vaccine is sheep pox virus and contagious pustular dermatitis virus cell passage attenuate virus or passage virus thereof which are separated through the inventor and obtained after passage attenuate culture, wherein the sheep pox virus cell passage attenuate virus is Sheeppox Virus GS-WW 2010 F44 strain, the preservation number of CCTCC NO: V201504, the sheep pox virus (contagious pustular dermatitis virus) cell passage attenuate virus is Orf Virus HB-TS09F65 strain, and the preservation number is CCTCC NO:V201406. Toxin attacking protection test results show that the sheep pox and aphtha bigeminy cell attenuated vaccine is safe and capable of producing effective immune protection force. Accordingly, the sheep pox and aphtha bigeminy cell attenuated vaccine is of an important practical significance in researching safe and efficient attenuated vaccine and preventing and controlling sheep pox and aphtha bigeminy.

The invention discloses a sheep aphthovirus Asial type multi-epitope recombinant vaccine and a preparation method thereof. The recombinant vaccine comprises the following ingredients: a protein encoded by aphthovirus multi-epitope genes and carrier protein fusion genes, and an aphthovirus 3D protein. The preparation method comprises the following steps: respectively diluting the protein encoded by the aphthovirus multi-epitope genes and the carrier protein fusion genes and the aphthovirus 3D protein; then, uniformly mixing the diluted proteins; adding auxiliary agents into the mixture; carrying out emulsification; and obtaining the sheep aphthovirus Asia1 type multi-epitope recombinant vaccine. Animal model and animal immune efficiency experiments show that the sheep Asial epitope recombinant vaccine of the invention can generate overall immunoprotection reaction, injected immune sheep can be induced to generate high-level neutralizing antibodies, and can also induce the cellullar immunologic response. The recombinant vaccine of the invention can effectively protect animals from the virulent strain attack of the aphthovirus.