A kind of rapid separation method of aphthous ulcer virus

Abstract

The invention relates to a method for rapidly separating a sore mouth disease virus and in particular relates to a method for separating the sore mouth disease virus from peripheral blood of ill sheep and recessive virus-carried sheep by utilizing bovine testicle cells. The sore mouth disease virus is separated from the peripheral blood of ill sheep suffering from the sore mouth disease and the recessive virus-carried sheep by utilizing the bovine testicle cells, a convenient and rapid method is provided for separating the sore mouth disease, and convenience is brought to production and scientific research. The method for rapidly separating sore mouth disease virus disclosed by the invention comprises the following steps: (1) collecting and detecting a sore mouth disease virus sample; (2) treating the sample; and (3) separating the sore mouth disease virus.

Description

1. Technical field [0001] The invention relates to a rapid separation method of aphthous aphthus virus, in particular to a method for isolating aphthous aphthous aphthus virus from the peripheral blood of diseased sheep and hidden virus-infected sheep by using bovine testicular cells. 2. Background technology [0002] Orf contagious impetigo, commonly known as Orf, is a zoonotic infectious disease caused by aphth virus in goats and sheep. The World Health Organization (IOE) lists the disease as an animal disease that needs to be declared. my country Classify it as a class 1 animal disease. The disease is distributed in almost all sheep-raising countries in the world. It mainly infects goats and sheep under natural conditions, and goats are more susceptible. The disease is often epidemic in groups. Lambs aged 3 to 6 months are most susceptible, and adult sheep are less prone to disease. Sick sheep and infected sheep are the source of infection of this disease. The virus can ...

AI Technical Summary

Problems solved by technology

[0004] The existing sheep aphthous ulcer virus is isolated from scabs and lip dander of sick sheep. It takes 5 generations of blind transmission before there will be lesions and scabs Both skin and dander need to be soaked and milled, which is time-consuming and laborious

At the same time, the collection of scabs is time-consuming, and severe cases will cause bleeding on the lips of sheep, which will affect the sheep's feed intake

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