215 results about "Testicle" patented technology

The invention belongs to the technical field of biological medicines, and particularly relates to a composition for treating male erectile dysfunction and application thereof. The oyster polypeptide is prepared by enzymolysis of oyster protein, can effectively promote proliferation of testicular interstitial cells, enhance and improve sexual desire and improve sexual behavior, has certain clinical application value, has the advantages of simple preparation, easy absorption and the like, and is easy to popularize and apply.

The invention relates to application of pirfenidone in preparation of testicular organs, and the pirfenidone can improve one or more properties and / or functions of the testicular organs, especially improve blood testis barriers and improve spermatogenesis efficiency. The invention also provides a method, a culture medium and a kit for preparing the testicular organoid.

Embodiments of the present invention provide a pharmaceutical intervention in the neonatal pig that inhibits and delays development and activation of the HPG axis, growth of the boar testis and inhibits testicular production of testosterone and androstenone, which prevents the development of aggressive behavior in the maturing boars and the presence of boar taint in the meat. The invention comprises treatment with a combination of an androgen and an estrogen in the newborn male piglet using extended drug delivery methods, with a defined duration of no more than 12 weeks, for the purpose of inhibiting the production of testosterone and androstenone and the accumulation of the boar taint-inducing molecules androstenone and skatole in the fat.

The invention relates to the technical field of biology, in particular to a swelling hippocampal peptide as well as a preparation method and application thereof. Comprising a polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The hippocampus antioxidative peptide is prepared by adopting an ultrahigh pressure pretreatment and enzymolysis technology, the adopted extraction process is simple in route, and the time and the production cost are effectively saved; an animal model is utilized to prove the antioxidation effect and the effect of improving male reproductive disorder and testis injury of the hippocampal swelling peptide; and on the basis, a novel peptide fragment with high activity, no toxicity and no sensitization is obtained.

The invention provides application of mitochondrial acid 5 in preparation of a medicine for treating reproductive organ injury caused by diabetes mellitus, and relates to the technical field of biological medicine. Experiments prove that as an artificially synthesized indole-3-acetic acid derivative, MA-5 provides a brand new treatment thought for testis injury caused by diabetes mellitus, not only is beneficial to improvement of reproductive function, but also possibly provides a new strategy for overall treatment of diabetes mellitus. Besides, as a natural plant hormone, MA-5 has relatively high safety and acceptability, and possibly becomes a new alternative therapy with a clinical application prospect. Although the long-term administration of the MA-5 does not significantly influence the blood glucose level, the alleviation effect of the MA-5 on testis injury shows that the MA-5 as a treatment means for long-term use can continuously improve reproductive dysfunction caused by diabetes, and has potential clinical advantages.

A fertility-enhancing cooling seat for lowering temperatures of human testicles so as to increase sperm quality and quantity. The cooling seat includes: a top flexible portion and a base portion; a bottom flexible portion coupled to the top flexible portion, the bottom flexible portion forming a seal with the base portion, portions of the bottom flexible portion and portions of the top flexible portion defining isolated cooling cells with cooling-cell volumetric spaces; a phase-change material located in the cooling-cell volumetric spaces, the phase-change material configured to change from a solid phase to a liquid phase at a first temperature and that is configured to change from a liquid phase to a solid phase at a second temperature that is lower than the first temperature. At least one of the isolated cooling cells defines a diameter that is larger than a diameter of the other of the cooling cells.

The invention relates to the technical field of biology, in particular to a swelling hippocampus antioxidative peptide as well as a preparation method and application thereof. Comprising a polypeptide with an amino acid sequence as shown in SEQ ID NO.1. The hippocampus antioxidative peptide is prepared by adopting an ultrahigh pressure pretreatment and enzymolysis technology, the adopted extraction process is simple in route, and the time and the production cost are effectively saved; an animal model is used for proving the antioxidation effect of the hippocampus erectus antioxidative peptide and the effect of improving male reproductive disorder and testis injury; and on the basis, a novel peptide fragment with high activity, no toxicity and no sensitization is obtained.

The invention relates to the technical field of reproductive medicine, in particular to a high-quality sperm acquisition method based on a testis tissue sample of an azoospermia patient, which comprises the following steps: inputting a first frame of a testis tissue sample microscopic video sequence into a target detection model, outputting a sperm detection frame and confidence, and taking the position of the detection frame as a starting point of a motion trail; a dynamic confidence matching strategy is adopted, firstly, a prediction trajectory is matched with a high-confidence detection result, then, a real leak detection target is complemented with a low-confidence target, multi-stage Hungary matching is achieved through confidence guidance, and all sperm movement trajectories obtained in each frame are obtained; and based on multiple frames of images corresponding to all sperm movement tracks corresponding to the microscopic video sequence, selecting the multiple frames of images according to a preset number of confidence coefficients to construct a candidate morphological analysis sample set, and performing kinematics parameter calculation and morphological analysis to obtain high-quality target sperms. According to the method, the recognition efficiency of high-quality sperms is improved, the sperms with higher quality can be obtained, and the subsequent fertilization success rate is further improved.

A lower body garment is disclosed having a sling assembly for securing male genitalia. The sling assembly is configured with limited, prescribed attachment points, which enables increased comfort and support, for the wearer. Moreover, the sling assembly provides a comfortable encapsulation that reduces the jostling of the testicles during athletic endeavors. The sling assembly configuration can further provide moisture management to reduce itching, chafing, and infection.

The invention discloses a porcine circular RNA, a porcine circLDHC, an overexpression vector and a construction method and application thereof, and relates to the technical field of livestock molecular biology. The porcine circular RNA is formed by cyclizing 3rd to 6th exons of an LDHC gene, and the nucleotide sequence of the porcine circular RNA is as shown in SEQ ID NO: 1. The porcine circLDHC is obtained by carrying out reverse transcription on porcine circular RNA (Ribonucleic Acid). According to the invention, a specific amplification primer is designed for circLDHC, and the real existence of circLDHC is verified through PCR (Polymerase Chain Reaction) amplification, sequencing and RNase R enzyme digestion. Fluorescent quantitative PCR analysis finds that circLDHC is highly expressed in sexual mature testis tissues of pigs, the expression level of circLDHC is related to testis maturation, and meanwhile, the expression quantity of circLDHC in testis of 75-day-old Meishan boars is remarkably higher than that of circLDHC in testis of same-day-old Duroc boars (plt; 0.01) of the substrate. The invention further finds that the over-expressed circLDHC inhibits the proliferation of the porcine sertoli cells, promotes the maturation of the porcine sertoli cells, lays a foundation for further researching the start of the puberty period of the boars, and provides a new thought for analyzing the sexual precocious puberty mechanism of the boars and improving the reproductive performance.

The invention discloses an experimental device and method for simulating rat reproduction influence under exposure of a low-pressure anoxic environment, relates to the technical field of reproduction influence experiments, and provides the following scheme that the experimental device comprises a low-pressure experimental module fixed on the ground and a vacuum pump erected on the top surface of the low-pressure experimental module, the extraction end of the vacuum pump penetrates into an inner cavity of the low-pressure experiment module through a pipeline, a hatch is formed in the left side of the low-pressure experiment module, and a hatch door is installed on the end face of the hatch through a hinge. According to the technical scheme, by simulating the day and night temperature difference at the altitude of 4000 m and 6000 m and 12 DEG C / 2 DEG C in the low-pressure experiment module and simulating the plateau environment to feed rats, the plateau low-oxygen exposure environment can be closer to the plateau low-oxygen exposure environment, and by conducting slice detection on testicular tissue of the test rats, damage data of the low-pressure low-oxygen exposure environment to the male reproductive system is obtained. Therefore, the method has certain good reproducibility on reproductive system injury in a low-pressure and low-oxygen exposure environment.

The invention discloses application of mangiferin in preparation of a protective agent for testicular sertoli cells and preparation of a medicine for treating male infertility diseases, and relates to the technical field of medicine application. Wherein the mangiferin can play a role in protecting the sertoli cells, and the protection role is reflected in ways of improving the cell viability of the sertoli cells, inhibiting the apoptosis of the sertoli cells, inhibiting the oxidative stress of the sertoli cells, inhibiting the inflammatory response of the sertoli cells and the like; meanwhile, mangiferin can be used for treating male infertility diseases in ways of relieving weight loss, repairing testis, improving endocrine system functions, inhibiting oxidative stress of testis tissues, inhibiting inflammatory response of the testis tissues and the like. Wherein the mangiferin can inhibit the inflammatory reaction mainly by inhibiting the activation of NLRP3 inflammasome to inhibit the NLRP3 pathway, so that the inflammation and even pyroptosis of testicular sertoli cells are remarkably reduced to improve spermatogenesis, and the male infertility disease is well treated.

The invention provides a functional peptide with a yang tonifying effect, a seal penis peptide and a preparation method and application of the functional peptide and the seal penis peptide. The functional peptide comprises a peptide fragment pER and can promote testicular interstitial cells to secrete testosterone. Testosterone is steroid hormone and can be secreted by testicular interstitial cells through enzymatic reaction. The low testosterone level in serum can cause male testicular atrophy and low sperm survival rate, so that the male reproductive function is influenced, and the phenomena of poor sexual desire, premature ejaculation, erectile dysfunction and the like can also be caused. In addition, the testosterone also has the effects of maintaining muscle strength and quality, maintaining bone density and strength, refreshing, improving physical ability and the like. Therefore, the functional peptide provided by the invention has the effect of promoting the secretion of testosterone, which means that the functional peptide not only can effectively improve the male sexual function, but also can alleviate the damage of male sexual dysfunction to the body and spirit.

The utility model discloses a paracentesis auxiliary device which is installed on supports on two sides of a sickbed body and comprises a first connecting rod and a second connecting rod which are respectively connected with the supports on the two sides of the sickbed body, and an adjusting cross beam is connected between the first connecting rod and the second connecting rod in a sliding mode. The outer wall of the adjusting cross beam is sleeved with a connecting sleeve, the two ends of the outer wall of the connecting sleeve are each sleeved with an elastic connecting belt, and the end, away from the connecting sleeve, of each elastic connecting belt is detachably connected with a binding net bag. The fixing device can be conveniently adjusted according to the positions of the testis and the penis of a patient, the application range is widened, the stability of the whole structure in the puncture operation process is enhanced, and therefore the better fixing effect is achieved.

The present invention relates to the technical fields of molecular biology, virology, immunology and medicine, and in particular to a castration vaccine having engineered ferritin and GnRH expressed in tandem, and a use. In the present invention, the engineered ferritin and different quantities of GnRH are linked in tandem and then recombined, so as to construct a GnRH-ferritin nanoparticle castration vaccine. The vaccine is used for castration of animals. Experimental results show that the GnRH and mutant ferritin recombined vaccine has a significantly enhanced immune effect and can significantly reduce testosterone levels and testicular weight, and compared with other combination methods, the nanoparticle castration vaccine having four GnRHs and the mutant ferritin linked in tandem has higher expression, stronger immunogenicity, faster immune response, and significant advantages in effect, and can be widely applied in the technical field of castration of animals.

The invention discloses application of liver-soothing and kidney-tonifying conceptive decoction in preparation of a medicine for treating hypothyroidism type asthenospermia. The liver-soothing and kidney-tonifying conceptive decoction is prepared from 12-18 g of tribulus terrestris, 12-18 g of radix curcumae, 12-18 g of fructus lycii, 12-18 g of semen cuscutae, 12-18 g of ligusticum wallichii, 8-12 g of deer-horn glue (molten), 8-12 g of raspberry, 8-12 g of flatstem milkvetch seed and 8-12 g of schisandra chinensis. The liver soothing and kidney tonifying conceptive decoction can repair testis oxidative stress injury, recover thyroid hormone level, regulate endoplasmic reticulum stress PERK / EIF2alpha / ATF4 signal channels and down-regulate pro-apoptotic protein CHOP expression level, so that spermatogenic cell apoptosis is relieved, and a new way is provided for preparation and development of drugs for treating male asthenospermia caused by hypothyroidism.

The invention discloses a method for generating haploid sperm-like cells through in-vitro differentiation of pluripotent stem cells and application of the haploid sperm-like cells. Human pluripotent stem cells are firstly differentiated into ectoderm-like cells, then the ectoderm-like cells are spontaneously gathered and converted into primordial germ cell-like cells under the induction of ectoderm induction factors, and then the primordial germ cell-like cells and testicular sertoli cells are subjected to mixed culture to form recombinant testicular tissues; the recombinant testis tissue enters meiosis under the induction of nutrition limitation and retinoic acid, then meiosis is completed under the stimulation of meiosis second-stage induction factors such as testosterone, follicle-stimulating hormone and pituitary extract, and interaction between primordial germ cell-like cells and testis supporting cells is enhanced by using small molecules SB431542, so that the meiosis of the testis is enhanced. And finally inducing to generate haploid sperm-like cells. According to the invention, a new tool can be provided for researching a molecular mechanism of male reproduction, and an important thought can be provided for research and transformation of asthenospermia, oligospermia, drug screening and the like.

This invention discloses a traditional Chinese medicine composition for treating male infertility due to liver stagnation and kidney deficiency, its preparation method, and its application, belonging to the fields of traditional Chinese medicine pharmaceutical technology and andrological reproductive health technology. The composition is formulated with Bupleurum chinense, Tribulus terrestris, Cynomorium songaricum, Cuscuta chinensis, Cornus officinalis, and Epimedium brevicornu in specific weight proportions, following the principles of soothing the liver and relieving stagnation, tonifying the kidney and replenishing essence, and unblocking the meridians to treat impotence. This invention employs an alcohol-water dual-phase fractional extraction process, and can be prepared into various dosage forms such as granules, capsules, and tablets. Pharmacological experiments have confirmed that the composition of this invention can increase sperm density and progressively motile sperm rate, improve penile tissue nitric oxide synthase (NOS) activity, regulate reproductive hormones, and reduce testicular oxidative damage; serum pharmacochemistry and metabolomics have clarified the active components entering the bloodstream and their pathways of action. This invention features a refined formulation, abundant medicinal resources, and simple preparation, making it suitable for large-scale production and clinical application in treating male infertility due to liver stagnation and kidney deficiency.

The invention belongs to the technical field of medicines, and particularly relates to a composition for relieving and repairing testis injury caused by heat stress and application of the composition. The composition for alleviating and repairing testis injury caused by heat stress comprises the following raw materials: a lycopene inclusion compound, ginsenoside Re, modified chitosan nanoparticles and a solvent, the use amount of the modified chitosan nanoparticles is 10 to 15 weight percent of the ginsenoside Re. A combined formula with lycopene and ginsenoside Re as cores is innovatively designed, the two main effective components in the formula are natural plant or traditional Chinese medicine extracts, conflicts between traditional Chinese medicine properties are effectively avoided, and the lycopene and ginsenoside Re combined formula has good biological safety and is suitable for being used as a traditional Chinese medicine preparation. The composition can be used for remarkably repairing testis injury caused by heat stress and accelerating recovery after heat stress injury.

The invention relates to the technical field of biological medicines, and particularly discloses application of ebbeselenium in preparation of a medicine for treating male delayed gonadal hypofunction. After the ebbeselenium is adopted for intervention, the autophagy activity of testicular interstitial cells of old mice is increased, lipid autophagy is enhanced, testosterone synthesis is promoted, the serum testosterone level is remarkably improved, and the symptoms of male delayed hypogonadism (LOH) are effectively relieved; ebbeselenium simultaneously shows a relatively good delaying effect on senescence of a plurality of organs. The ebbeselenium can improve the reproductive capacity, the behavior memory capacity, the athletic capacity and the metabolic function of old mice at the same time, and synergistic and effective treatment of low serum testosterone and LOH complications is achieved. Besides, by enhancing the molecular reduction effect of PRDX1 targeting ATG4B, the ebbeselenium regulates and controls the autophagy pathway of testicular interstitial cells and enhances the functions of the testicular interstitial cells so as to promote synthesis of testosterone, and a new treatment strategy is provided for treatment of LOH.

A method of treating male hypogonadism in a subject in need thereof includes administering to the subject testicular organoids generated from isolated male progenitor cells. The method can be used to slow or halt the progression of age-related diseases, and increase healthspan and longevity, in hypogonadal males. The method can be used with subjects that have not or are not receiving testosterone replacement therapy. The method does not require that the subject is pre-treated with CG. The testicular organoid includes cells expressing GFRA1, VEGF, VEGFR2, PDGF-Rα, Cyp11A1, 3β-HSD, 17β-HSD, LHCGR, α-SMA, CD11b, MHC CII, CD64, CD95, Sox9, AR, inhibin β-B, and FSHR.

This invention provides a sauna testicular cover that can be stably placed on the seat of a sauna room and protect the user's testicles from excessive heat. [Solution] The sauna testicle cover comprises a bottom portion having a flat outer bottom surface, a wall portion that rises outward in a convex shape from around the bottom portion, and an opening formed at the upper end of the wall portion. This sauna testicle cover is configured to accommodate the user's testicles, which are received through the opening, inside the bottom portion and wall portion when the outer bottom surface is in contact with the seat surface of the sauna room. With this sauna testicle cover, the user's testicles can be protected from excessive heat while stably placed on the seat surface of the sauna room.

The invention provides an application of ergothioneine as a testis oxidative stress regulator. The invention provides a multi-target intervention method based on ergothioneine, which systematically improves sperm quality reduction caused by obesity by cooperatively regulating key links such as oxidative stress level, hormone secretion state and mitochondrial function. According to the invention, a safe and effective intervention effect with a clear action mechanism can be realized, and a new technical approach is provided for the treatment of obesity-related male reproductive dysfunction. The invention also provides application of the ergothioneine as the testicular steroid generation accelerant.

The utility model discloses an efficient testicular puncture tissue scraping device which comprises a scraping device and a containing vessel used for containing testicular puncture tissue. The scraper comprises a handle and a cutting head, the cutting head is arranged at one end of the handle and comprises a horizontally-arranged fixing plate, the fixing plate is fixed to the handle, a plurality of cutting blades are fixedly arranged on the lower surface of the fixing plate, the plane where all the cutting blades are located is perpendicular to the fixing plate, and cutting edges of the cutting blades face downwards. Meanwhile, all the cutting blades are arranged side by side in the longitudinal direction of the handle, so that the cutting blades on the cutting head are conveniently driven to move front and back by holding the handle, and the testicular puncture tissue in the containing vessel is scraped. The testicular puncture tissue scraping device is convenient to operate, can efficiently scrape testicular puncture tissues, and is favorable for reducing the labor intensity of operators and improving the working efficiency.

A lower body garment is disclosed having a sling assembly for securing male genitalia. The sling assembly is configured with limited, prescribed attachment points, which enables increased comfort and support, for the wearer. Moreover, the sling assembly provides a comfortable encapsulation that reduces the jostling of the testicles during athletic endeavors. The sling assembly configuration can further provide moisture management to reduce itching, chafing, and infection.

The application of multi-effect molecular markers that simultaneously predict chicken abdominal fat traits, leg muscle traits and reproductive traits belongs to the field of animal molecular genetics technology. In order to study the influence of LDAH gene on chicken abdominal fat, leg muscle and testicle, the present invention designs primers according to the c.43346_43347insTGTGTCTT mutation site of chicken LDAH gene, performs PCR amplification of chicken genomic DNA on F2 population, electrophoresis, and detects 2 genotypes in F2 population (AA rooster and white-ear hen hybrid construction), and proves that its influence on abdominal fat rate and leg muscle rate reaches extremely significant level; The influence on testicle rate reaches significant level, proving that chicken LDAH gene mutation site c.43346_43347insTGTGTCTT is a molecular marker that affects the growth and development of chicken abdominal fat, leg muscle and testicle. The molecular marker can be used to select chicken abdominal fat content, leg muscle weight and testicle weight at the same time.

Disclosed herein are methods of reducing pathological inflammation and effects thereof through administration of tissues associated with regenerative and / or tolerogenic activity. In some embodiments, such tissues can include placental, ocular, testicular, and osseous tissues. Augmentation of therapeutic effects of said tissues may be achieved through contact with Injury Mimetics.