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56 results about "Testis tissue" patented technology
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Seminiferous tubules are coiled tubes that make up most of each testis. The cells and tissues in the tubules are responsible for spermatogenesis, which is the process of creating sperm. These tubules are lined with a layer of tissue called the epithelium.
Method for separating and purifying chicken's spermospore
The method of separating and purifying chickení»s spermatospore relates to trnasgene, rare species preservation, tissue and cell engineering, and other fields. The present invention includes first separating chickení»s spermatospore and subsequent Percoll separation and adherent purification. The present invention provides proper chickení»s spermatospore separating and purifying method by means of separating chickení»s spermatospore from chickení»s testis tissue, and Percoll gradient and adherent purification, spermatogenic epithelium cell suspension with great testis cell number and high survival rate of testis cell is prepared while ensuring elimination of other somatic cell components.
Owner:YANGZHOU UNIV
Eriocheir sinensis EsSXL gene, amplification primer group thereof and amplification method
The invention discloses an Eriocheir sinensis EsSXL gene, an amplification primer group thereof and an amplification method. A full-length cDNA (complementary Deoxyribose Nucleic Acid) sequence of the EsSXL gene is cloned from an Eriocheir sinensis testis tissue by using the primer group provided by the invention, and the EsSXL gene comprises two variable spliceosomes EsSXL1 and EsSXL2, and lays an important foundation for the research of the important function of the function of the Eriocheir sinensis EsSXL gene in a sex differentiation process of the Eriocheir sinensis EsSXL gene.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
Method for in vitro culture of testis tissues and induction to generate spermatid
The invention relates to the field of cell engineering, and concretely provides a method for in vitro culture of testis tissues and induction to generate spermatid. The method comprises the following steps: 1, preparing culture gel pieces: placing 1.2% agarose gel pieces in a 6-orifice plate, adding a medium, immersing overnight, extracting the used medium, adding the fresh medium, and using the agarose gel pieces as culture gel pieces, wherein the medium comprises 90% of MEM alpha and 10% of KSR; 2, taking 5.5-days and 10.5-12.5-days mouse testes, removing external layer albuginea, and cutting to form block tissue samples; 3, placing the tissues samples on the culture gel pieces, putting the 6-orifice plate in a cell culture box, and culturing; and 4, culturing the 5.5-days mouse testis tissues for 22-25d to obtain round sperms, and culturing the 10.5-12.5-days mouse testis tissues for about 27d to obtain elongated sperms.
Owner:CHINA AGRI UNIV
Composition having functions of relieving fatigue and tonifying yang
The invention discloses a lycium barbarum polysaccharide-deer blood composition having functions of relieving fatigue and tonifying yang. Lycium barbarum polysaccharide and a deer blood dry powder asactive components are made into an oral preparation in accordance with conventional excipients and processes of the pharmaceutics, or are made into a food prepared for a meal in accordance with a health-care food process. Experiments show that the composition can significantly prolong the loaded swimming time of small mice, reduce the content of blood lactic acid and serum urea nitrogen after thesmall mice do exercise and increase the reserve amount of hepatic glycogen; compared with the fatigue relieving function of the single use of lycium barbarum polysaccharide and deer blood, the function is more comprehensive, and the effect is more effective. The composition also can significantly enhance the gonadal hormone level of rats and the activity of superoxide dismutase in testis tissues,reduces the content of malondialdehyde, enhances the antioxidant capacity of the rat, and has stronger activity than the activity of lycium barbarum polysaccharide and deer blood groups. Therefore, the composition can be used for enhancing the anti-fatigue and yang-tonifying ability of sub-health young and middle-aged men, as well as conditioning middle-aged and elderly patients with physical deficiency.
Owner:LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Method for isolating and identifying sperm cells of Buffalo testis
InactiveCN109182254ACertified pureGood identification effectCell dissociation methodsMicrobiological testing/measurementSingle cell suspensionHyaluronidase
The invention belongs to the technical field of sperm cell treatment, and specifically relates to a method for isolating and identifying sperm cells of Buffalo testis, including the following steps: obtaining Buffalo testis, digest with collagenase IV, hyaluronidase and DNase I combined enzyme, sieving, filtering, and taking suspension cells after differential adherence; 2) taking the suspend cells for live cell stain; and 3) separating by flow cytometry. Beneficial effect: the technical scheme is an effective method for separating buffalo sperm cells, By digesting bovine testis tissue with two enzymes, so that a single cell suspension is obtain, the interference of somatic cells is removed by the method of continuous differential adherence, according to the fluorescence intensity of non-adherent cells stained by flow cytometry, the DNA content of cells is judged, and the interference of other non-target cells such as diploid spermatogonia and tetraploid primary meningoblasts is effectively digested and removed to achieve the successful sorting of a large number of haploid spermatozoa.
Owner:GUANGXI UNIV
Application of astaxanthin in preparation of drugs controlling organophosphorus pesticide induced male reproductive toxicity
ActiveCN105434408AMale reproductive toxicity is goodEnhanced inhibitory effectOrganic active ingredientsSexual disorderAnimal sciencePhoxim
Owner:XINXIANG MEDICAL UNIV
Establishment and induced differentiation method of spermatogonial stem cell line of Chinese sword fish
ActiveCN113025565AStem cells perform wellCell dissociation methodsClimate change adaptationBiotechnologyStem cell line
Owner:SHANGHAI OCEAN UNIV
Takifugu flavidus protamine extraction method
InactiveCN109912708AThe method of extraction is reasonable and feasibleHigh extraction ratePeptide preparation methodsAnimals/human peptidesAlcoholTakifugu flavidus
The invention provides a takifugu flavidus protamine extraction method. Extraction process conditions include that pH of 0.14mol / L of NaCl solution is 8.5, the concentration of sulfuric acid solutionis 0.6mol / L, consumption of the sulfuric acid solution is 4.0 volumetric times of a precipitant, an extraction temperature is 45 DEG C, extraction time is 2.4h, consumption of ethyl alcohol is 4 volumetric times of supernatant, and the actual extraction rate of protamine is 19.07%. Extracting protamine from takifugu flavidus testes provides an experimental foundation for research on physicochemical properties and related functional characteristics of protamine, and a function of effective utilization of takifugu flavidus testis tissues can be achieved.
Owner:FISHERIES RES INST OF FUJIAN
Extraction method of globefish testis milt components
InactiveCN110760509AImprove utilizationReduce usagePeptide preparation methodsAnimals/human peptidesUltrafiltrationMilt
The embodiment of the invention discloses an extraction method of globefish testis milt components. The extraction method of the globefish testis milt components includes the steps: globefish testes are pretreated, and scattered testis cells are obtained; ultrasonication is conducted on the scattered testis cells, and filtration and separation are carried out to obtain white precipitate; a set volume of concentrated hydrochloric acid is added to dissolve the white precipitate, and then a solution is formed; the solution passes through an ultrafiltration membrane, and then a trapped liquid anda filtrate are obtained separately; extraction is conducted on the trapped liquid, and then milt nucleic acids are obtained; and extraction is conducted on the filtrate, and then milt proteins are obtained. According to the extraction method of the globefish testis milt components, by utilizing the globefish testes as an extraction raw material, a large quantity of globefish viscus wastes producedby a globefish culture industry can be effectively utilized. Besides, according to the technical scheme, separation and extraction of the milt DNA and the milt proteins are achieved, thus, the availability of testis tissue can be improved, usage of surface active agents and organic solvents is also reduced, and therefore the extraction method of the globefish testis milt components is more environmentally friendly.
Owner:广州市藻德道科技有限公司
Application of geniposide in preparation of medicine for treating asthenospermia
ActiveCN113768945ASuppress stressRaise the potentialOrganic active ingredientsPharmaceutical delivery mechanismReticulum cellBiophysics
The invention provides application of geniposide in preparation of a medicine for treating asthenospermia. Experimental results show that the geniposide can significantly improve the sperm quantity and activity and reduce the sperm malformation rate, can significantly inhibit endoplasmic reticulum stress inside testis, increase mitochondrial membrane potential, regulate expression of related proteins in testis tissues, and improve excessive apoptosis of spermatogenic cells.
Owner:NINGXIA MEDICAL UNIV
Processing method of animal testis tissue powder
The invention relates to the technical field of food processing, in particular to a processing method of animal testis tissue powder. The method mainly comprises the following steps of: step 1, selecting testes of animals which reach sexual maturity and are slaughtered after passing quarantine inspection as a raw material; step 2, removing fascia tissue, blood dirt and impurities on the surface ofthe raw material; step 3, cutting the raw material to generate a granular material; step 4, carrying out low-temperature quick freezing on the granular material; step 5, carrying out vacuum freeze drying on the granular material; step 6, finely grinding the granular material to obtain a powdery finished product; and step 7, packaging and storing the finished product. Aiming at the defects of existing animal testis storage and processing methods, the method provides a testis powder product which is convenient to store, can retain testosterone and immunoglobulin substances and accords with theconsumption habits of modern people.
Owner:姚贵勇 +1
Application of betulinic acid serving as body cadmium scavenging agent
ActiveCN106074562AAvoid damageReduce cadmium residuesOrganic active ingredientsAntinoxious agentsTestis tissuePharmacology
The invention relates to novel application of betulinic acid, in particular to application of betulinic acid in preparing drugs, health-care products and foods for clearing away cadmium in a body. It is verified through in-vitro and in-vivo tests that betulinic acid does not have obvious toxic and side effects on normal cells and mice, residues of cadmium in the mouse liver, kidney and testis are reduced, and discharge of cadmium in urine can be promoted; mouse liver, kidney and testis tissue damage caused by cadmium can be reduced. Betulinic acid has the effect of treating body cadmium accumulation and is safe and effective, betulinic acid is superior to a traditional therapeutic drug, the application range of betulinic acid is widened, and a new way is provided for treating heavy metal cadmium poisoning.
Owner:WUHAN UNIV
Establishment method for differentiating swine germline stem cells into sperms in vitro
InactiveCN105624101APromote meiosisEasy to operateCulture processCell culture active agentsFibroblastFeeder Layer
The invention discloses an establishment method for differentiating swine germline stem cells into sperms in vitro. The establishment method includes the steps of subjecting piglet testis tissues to isolation culture to obtain primary piglet testis tissue fibroblasts and germline stem cells, culturing the testis tissue fibroblasts for 15 days by means of a Niche culture medium for inducing in-vitro differentiation of sperms to obtain a Niche feeder layer for inducing in-vitro differentiation of sperms, and inoculating the germline stem cells to the Niche feeder layer to culture for 27-30 days, thereby obtaining haploid sperm-like cells in vitro by cytobiology analysis. The establishment method is easy to operate and good in repeatability, is an important breakthrough in research on swine mGSCs(male germline stem cells) in-vitro differentiation, provides an important basic technology for researches on swine embryonic in-vitro operation and transgenic breeding, and meanwhile, has high reference value in research on in-vitro differentiation of sperms of other species and assisted reproduction of human beings.
Owner:SOUTH CHINA AGRI UNIV
Highly-integrated and intelligent testis paracentesis cooperating platform
InactiveCN110384596AEasy to operateLittle effortOperating tablesSurgical needlesVehicle frameParacentesis
The invention belongs to the technical field of medical equipment, and particularly relates to a highly-integrated and intelligent testis paracentesis cooperating platform which comprises a cart frameand an operating table device. The cart frame comprises a cart body, wheels and a collecting box, the wheels are arranged at the bottom of the cart body, and the collecting box is arranged on the cart body; the operating table device comprises a table body, telescopic rods, a stand column, a table lamp, a tray and a surgery device, the table body is supported by the cart body through the four telescopic rods, the stand column is arranged on the edge of the left side of the table body, the table lamp is arranged at the top end of the stand column, the tray is arranged on the right side of thetable top of the table body, and the surgery device comprises a testis tissue separating device and a testis tissue taking-out device, wherein the testis tissue separating device is arranged on the front edge of the table body, and the testis tissue taking-out device is arranged on the rear edge of the table body. The highly-integrated and intelligent testis paracentesis cooperating platform has rapidness, convenience and flexibility in testis paracentesis of medical staff.
Owner:LIUZHOU CITY HEALTHCARE HOSPITAL FOR WOMEN & CHILDREN
Reproduction marker gene vasa of Sinopotamon henanense and application thereof
InactiveCN107267536AConfirm reproductive specificityFast and accurate identificationHydrolasesMicrobiological testing/measurementNucleotideNucleotide sequencing
The present invention discloses Henan Huaxi crab (Sinopotamon henanense) reproductive marker gene vasa and its application, the nucleotide sequence of the gene is shown in the sequence table SEQ ID NO.1; also discloses the protein encoded by Henan Huaxi crab gene vasa Sequence, as shown in the sequence table SEQ ID NO.2, and the physical and chemical properties of the protein, the characteristics of amino acid residues, and the characteristics of the protein structure and functional domains. The coding region of the gene starts at the 142nd nucleotide at the 5' end and ends at the 2214th nucleotide, encoding 690 amino acid residues. The reproductive marker gene of Henan Huaxi crab of the present invention is specifically expressed in the ovary and testis tissue of Henan Huaxi crab through identification, and can be used for the development and application of reproductive marker molecules of Henan Huaxi crab and its similar species, and can be used to guide river crab Artificial breeding has practical significance.
Owner:SHANXI UNIV
Application of Hydrogen Sulfide and Its Donors in Improving Sperm Quality and Function
InactiveCN103735571BReduce stress damageMaintain vitality levelSulfur/selenium/tellurium inorganic active ingredientsSexual disorderNormal fertilityStress injury
Owner:昭远制药有限公司
Effect and application of plant fruit pulp in protection of male fertility
PendingCN114288329AImproved male fertilityFunction increaseSexual disorderFood scienceAnimal scienceZoology
The invention provides a novel application of plant fruit pulp. The invention relates to application of plant fruit pulp in testis function protection, application of plant fruit pulp in prevention and / or treatment of oligospermia and asthenospermia, application of plant fruit pulp in protection of male fertility and application of plant fruit pulp in prevention and / or treatment of testis tissue lesion, testosterone level decline, testis function decline, sperm number reduction, asthenospermia and asthenospermia caused by metabolic disorder. The sperm motility rate is reduced; the sperm morphology is abnormal. The plant fruit pulp can effectively prevent and treat symptoms such as testicular tissue lesion, testosterone level decline, testicular hypofunction, sperm number reduction, sperm motility rate reduction and sperm morphology abnormality caused by metabolic disorder. The application provides a basis for protecting testis functions, preventing and / or treating oligospermia and asthenospermia and protecting male fertility by directly utilizing the plant fruit pulp.
Owner:GENERAL HOSPITAL OF NINGXIA MEDICAL UNIV +1
Proliferative Primary Human Sertoli Cell Cultures And Their Applications
InactiveUS20090028833A1Expansion of the populationBiocideGenetically modified cellsEx vivo gene therapySertoli cell
Technology for the isolation and propagation of primary human Sertoli cells from normal testes tissue, including cultures of proliferative primary human Sertoli cells for research and clinical applications, and a pharmaceutical composition for cell therapy, ex vivo gene therapy, and for the reduction of autoimmune, allograft, and xenograft immune reactions.
Owner:MANDALMED
Application of compound 6-furfuryl amino purine in preparing medicine for treating testis senility
InactiveCN101066271AImprove antioxidant capacityPowder deliveryAntinoxious agentsAntioxidant capacitySerum ige
The present invention discloses application of compound 6-furfuryl amino purine in preparing medicine for treating testis senility. It is proved by means of experiment that compound 6-furfuryl amino purine as one kind of exogenic purine matter can raise the antioxidase activity of the testis tissue of senile mouse, raise the ability of body's eliminating free radical, reduce the damage of cell caused by lipoid preoxidation, raise the antioxidant capacity of mouse's testis and raise the serum testosterone level of senile mouse, improve sex gland function and delay senility of sex gland.
Owner:NORTHWEST A & F UNIV
Molecular breeding method based on polymorphism of candidate gene PRL (Prolactin)
InactiveCN102465179AAccurate judgmentMicrobiological testing/measurementAnimal husbandryBiotechnologyGenetic molecular
The invention provides a molecular breeding method based on the polymorphism of a candidate gene PRL (Prolactin) and relates to the technical field of poultry breading, in particular to a method for utilizing the polymorphism of the candidate gene PRL to perform molecular breeding. Under the condition of focusing on a gene effect of a PRL gene in a gonadal axis of a chicken, i.e. hypothalamus-hypophysis-ovary or testis tissue to reproductive characters of the chicken, mRNA differential expression or difference of translating proteins thereof, factors influencing the expression of the reproductive characters of the chicken are researched and a genetic molecular mechanism of the reproductive characters of the chicken is primarily illustrated, so that a function of the candidate gene during a reproductive developing process can be objectively understood, and meanwhile, the analysis to the influence thereof on a reproductive performance can be facilitated, a basis for marking auxiliary breeding can be provided, the improvement of the reproductive characters can be quickened and the potential economic benefit of the candidate gene can be utilized.
Owner:YANGZHOU JINMIAO AGRI TECH
Method for detecting nucleoproteins of spermatogenic cells through utilizing indirect immunofluorescent staining
InactiveCN103235137ARapid expansionProtection maintenanceBiological testingSucroseConvoluted seminiferous tubules
A method for detecting the nucleoproteins of spermatogenic cells through utilizing indirect immunofluorescent staining comprises the following steps: separating convoluted seminiferous tubules from the testis tissues of an animal, utilizing a hypotonic solution to process spermatogenic cells, and detecting the nucleoproteins of the spermatogenic cells through adopting indirect immunofluorescent staining, wherein the hypotonic solution comprises 17mM of sodium citrate, 50mM of sucrose, 5mM of EDTA, 0.5mM of DTT, 0.5mM of PMSF and 30mM of Tris and having a pH value of 8.2. The method uses the hypotonic solution to carry out permeable treatment of the cells in order to rapidly expand the cells, disperse chromatins and fully release the proteins in cell nuclei, so the spermatogenic cells can well combine with primary antibodies, thereby the combination efficiencies of secondary antibodies are enhanced, fluorescent staining is complete and full, and the preparation of spermatogenic cell chromosomes and karyotyping are successfully carried out.
Owner:SHANXI MEDICAL UNIV
Cancer-testis antigens
InactiveUS20060089303A1Effective treatmentEffective diagnosisOrganic active ingredientsTumor rejection antigen precursorsAbnormal tissue growthT lymphocyte
Cancer-testis (CT) antigens have been identified by screening public databases for transcripts that are expressed in tumor tissues and a limited set of normal tissues, or by screening for genes that are expressed in cancer and testis tissues (but not other normal tissues). The invention relates to nucleic acids and encoded polypeptides which are CT antigens expressed in patients afflicted with cancer. The invention provides, inter alia, isolated nucleic acid molecules, expression vectors containing those molecules and host cells transfected with those molecules. The invention also provides isolated proteins and peptides, antibodies to those proteins and peptides and cytotoxic T lymphocytes which recognize the proteins and peptides. Fragments of the foregoing including functional fragments and variants also are provided. Kits containing the foregoing molecules additionally are provided. The molecules provided by the invention can be used in the diagnosis, monitoring, research, or treatment of conditions characterized by the expression of one or more CT antigens.
Owner:CORNELL RES FOUNDATION INC +1
Specific marker screening method for detecting azoospermia and severe oligospermia
PendingCN113584148AImprove throughputImprove stabilityMicrobiological testing/measurementBiological testingProteomics methodsMetabolite
The invention belongs to the technical field of medical detection, and discloses a specific marker screening method for detecting azoospermia and severe oligospermia. The method comprises the following steps: collecting a sample, treating the sample, and screening out specific markers of the severe oligospermia and azoospermia; in a testicular tissue level, screening expression of differential microRNA in testicular tissues of normal males and sterile males by using a chip method; in a seminal plasma layer, searching differential marker proteins of normal males and sterile males by using a proteomics method; in the urine level, employing metabonomics for screening the difference of metabolites in urine of normal men and infertility men; integrating data obtained from three high-flux layers, namely a testis tissue layer, a seminal plasma layer and a urine layer, and carrying out correlation analysis on the data and sperm quality, density and vitality; and optimizing and establishing a diagnosis technology. The method is long in detection time, high in efficiency and capable of explaining the cause of dyszoospermia of the patient.
Owner:JILIN UNIV
Method for identifying physiological sex of fish at early development stage by using 5SrRNA
The invention discloses a method for identifying the physiological sex of fish at an early development stage by using 5S rRNA, which comprises the following steps: (1) extracting the total RNA of fish gonad tissues by using a method for conventionally extracting the total RNA of animal cells by using a total RNA extraction kit or a TRIzol reagent, determining an OD260 / 280 value by using a nucleic acid analyzer, and judging the RNA purity, and then separating total RNA by using an agarose gel electrophoresis method, and observing the brightness and distribution of 5S rRNA, 18S rRNA and 28S rRNA; (2) carrying out microcapillary electrophoresis on the extracted gonad total RNA, and calculating the proportion of 5S rRNA in the total RNA according to the RNA type coverage area obtained by nucleic acid analysis software; (3) according to the proportion of the 5S rRNA in the total RNA, judging the physiological male and female of the fish at the early development stage, when the proportion of the 5S rRNA is smaller than or equal to 9.0%, judging the fish as a testis tissue, and when the proportion of the 5S rRNA is larger than or equal to 12%, judging the fish as an ovarian tissue. The method is easy to implement and simple and convenient to operate, the physiological sex identification of more than 100 fish individuals is realized within 12 hours, and the physiological sex of the fish is determined.
Owner:HUAZHONG AGRI UNIV
In-vitro separation method for primary spermatogenic cells of rats
PendingCN113652394AThe edge halo is obviousCutting costsCell dissociation methodsGerm cellsSingle cell suspensionIn vitro study
The invention discloses an in-vitro separation method for primary spermatogenic cells of rats. The method comprises the following steps: 1) collecting the testis on two sides of a fresh in-vitro rat, and stripping envelopes and blood vessels to obtain testis tissues; 2) digesting the testicular tissue to prepare a single-cell suspension; 3) culturing the single-cell suspension until non-spermatogenic cells are adhered to the wall, sucking out non-adhered suspension cells, and separating the suspension cells by adopting a Percoll method to obtain the spermatogenic cells. According to the method, a foundation is laid for in-vitro research on the reproductive function of the rat testis, in-vitro separation and culture of the rat testis spermatogenic cells are achieved, and it is proved that the spermatogenic cells separated and cultured through the method are high in in-vitro survival rate and long in survival time. Normal rat testicular spermatogenic cells can be obtained by using the method, the cost is low, the operation is simple, the repeatability is high, various experimental requirements can be met, and the method is worthy of large-scale popularization.
Owner:XI AN JIAOTONG UNIV
Method for preparing cynoglossus semilaevis testis single-cell suspension
ActiveCN114381422AEasy accessEfficiently obtainedCell dissociation methodsArtificial cell constructsSingle cell suspensionDigestion
The invention relates to a method for preparing cynoglossus semilaevis testis single-cell suspension, and belongs to the field of cytobiology, and the method comprises the following steps: taking cynoglossus semilaevis testis tissues, flushing the tissues with a DMEM-Nacl solution, and then chopping and homogenizing; adding an enzyme mixed solution into the tissue homogenate, carrying out enzymolysis digestion in a water bath at 30 DEG C for 5-10 minutes, and observing under a microscope until tissue blocks basically disappear; filtering the digested cell suspension with a 40 [mu] m cell sieve; centrifuging the filtrate for the first time, collecting cell precipitates, adding a PBS-Nacl solution to resuspend cells, centrifuging for the second time, and repeating twice; and adding a BSA-PBS-Nacl solution with a volume ratio of 0.1% into the washed cell precipitate, and suspending the cells again to obtain the cynoglossus semilaevis testis single-cell suspension. According to the method, the cynoglossus semilaevis testis single-cell suspension is obtained, the dissociated cell concentration is ensured to be 1000-2000 cells / mu L, and the motility rate is greater than 80%.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Bovine testis tissue serum-free cryopreservation protective agent for bovine testis tissues, and cryopreservation method and application thereof
PendingCN111919836AComplete structureLow costDead animal preservationSomatic cellDisease causative agent
The invention is suitable for the technical field of biological cryopreservation, and provides a bovine testis tissue serum-free cryopreservation protective agent and a cryopreservation method and application thereof. The serum-free cryopreservation protective agent comprises the following components: a Knockout DMEM medium, a KSR serum substitute, dimethyl sulfoxide and mycillin. According to theinvention, the Knockout DMEM culture medium which does not contain serum is used as a substrate, the kSR serum substitute, dimethyl sulfoxide and mycillin are used as low-temperature protective agents in a combined manner, the cost of the cryopreservation protective agent can be reduced, the risk of exogenous pathogen and virus pollution is avoided, in addition, the bovine testis tissue can be preserved at low temperature for a long time, cell apoptosis is reduced, the tissue form is maintained, spermatogenic cells and testis cells are well protected, the testis tissue after cryopreservationand resuscitation is complete in structure, the activity of various testis cells is high, and normal proliferation and development can be achieved.
Owner:JILIN UNIV
Application of retinoic acid in preparation of drugs for improving prognosis of cryptorchidism
InactiveCN109223752AImprove infertilityImproves oxidative stress levelsHydroxy compound active ingredientsSexual disorderOxidative stressApoptosis
The invention relates to the application of retinoic acid in preparation of drugs for improving prognosis of cryptorchidism, and belongs to the technical field of medicine. The drug is mainly composedof retinoic acid, which can improve the infertility of cryptorchidism patients, and can improve the spermatogenesis function of cryptorchidism patients by improving the oxidative stress level and apoptosis level of cryptorchidism patients' testis tissue and the expression level of blood-testis barrier-associated protein. This provides a basis for improving the clinical treatment strategy for cryptorchidism and improving the prognosis of cryptorchidism.
Owner:CHILDRENS HOSPITAL OF CHONGQING MEDICAL UNIV
Method for separating and purifying chicken's spermospore
InactiveCN100366734CHigh purityMeet the needs of general testingGerm cellsPurification methodsTesticle
The method of separating and purifying chicken's spermatospore relates to trnasgene, rare species preservation, tissue and cell engineering, and other fields. The present invention includes first separating chicken's spermatospore and subsequent Percoll separation and adherent purification. The present invention provides proper chicken's spermatospore separating and purifying method by means of separating chicken's spermatospore from chicken's testis tissue, and Percoll gradient and adherent purification, spermatogenic epithelium cell suspension with great testis cell number and high survival rate of testis cell is prepared while ensuring elimination of other somatic cell components.
Owner:YANGZHOU UNIV
Preparation method of slide for specific spermatogenic cells at development stage
InactiveCN101963695BThe experimental results are comprehensiveExperimental results are objectivePreparing sample for investigationExpression FeatureTubule
The invention discloses a preparation method of a slide for specific spermatogenic cells at a development stage, and the method comprises the following steps: removing the envelope of a testis tissue which is taken from an animal, dispersing contorted senimiferous tubules, determining staging of a part corresponding to a spermatogenic epithelium wave mode according to the light transmission difference of the contorted senimiferous tubules under a dissecting microscope, then selecting an interesting part section to prepare the slide, and fixing the slides by 1wt% of paraformaldehyde solution after being frozen in liquid nitrogen, thus preparing the required slide for the specific spermatogenic cells. The method has the maximum advantage that the living cellular level of related molecules in the spermatogenic process is detected in vitro, complete, objective and precise experimental results can be obtained, and the results reflect expression features of a specific gene product of the spermatogenic cells at the development stage.
Owner:SHANXI MEDICAL UNIV
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