56 results about "Testis tissue" patented technology

The method of separating and purifying chickení»s spermatospore relates to trnasgene, rare species preservation, tissue and cell engineering, and other fields. The present invention includes first separating chickení»s spermatospore and subsequent Percoll separation and adherent purification. The present invention provides proper chickení»s spermatospore separating and purifying method by means of separating chickení»s spermatospore from chickení»s testis tissue, and Percoll gradient and adherent purification, spermatogenic epithelium cell suspension with great testis cell number and high survival rate of testis cell is prepared while ensuring elimination of other somatic cell components.

The invention discloses an Eriocheir sinensis EsSXL gene, an amplification primer group thereof and an amplification method. A full-length cDNA (complementary Deoxyribose Nucleic Acid) sequence of the EsSXL gene is cloned from an Eriocheir sinensis testis tissue by using the primer group provided by the invention, and the EsSXL gene comprises two variable spliceosomes EsSXL1 and EsSXL2, and lays an important foundation for the research of the important function of the function of the Eriocheir sinensis EsSXL gene in a sex differentiation process of the Eriocheir sinensis EsSXL gene.

The invention relates to the field of cell engineering, and concretely provides a method for in vitro culture of testis tissues and induction to generate spermatid. The method comprises the following steps: 1, preparing culture gel pieces: placing 1.2% agarose gel pieces in a 6-orifice plate, adding a medium, immersing overnight, extracting the used medium, adding the fresh medium, and using the agarose gel pieces as culture gel pieces, wherein the medium comprises 90% of MEM alpha and 10% of KSR; 2, taking 5.5-days and 10.5-12.5-days mouse testes, removing external layer albuginea, and cutting to form block tissue samples; 3, placing the tissues samples on the culture gel pieces, putting the 6-orifice plate in a cell culture box, and culturing; and 4, culturing the 5.5-days mouse testis tissues for 22-25d to obtain round sperms, and culturing the 10.5-12.5-days mouse testis tissues for about 27d to obtain elongated sperms.

The invention discloses a lycium barbarum polysaccharide-deer blood composition having functions of relieving fatigue and tonifying yang. Lycium barbarum polysaccharide and a deer blood dry powder asactive components are made into an oral preparation in accordance with conventional excipients and processes of the pharmaceutics, or are made into a food prepared for a meal in accordance with a health-care food process. Experiments show that the composition can significantly prolong the loaded swimming time of small mice, reduce the content of blood lactic acid and serum urea nitrogen after thesmall mice do exercise and increase the reserve amount of hepatic glycogen; compared with the fatigue relieving function of the single use of lycium barbarum polysaccharide and deer blood, the function is more comprehensive, and the effect is more effective. The composition also can significantly enhance the gonadal hormone level of rats and the activity of superoxide dismutase in testis tissues,reduces the content of malondialdehyde, enhances the antioxidant capacity of the rat, and has stronger activity than the activity of lycium barbarum polysaccharide and deer blood groups. Therefore, the composition can be used for enhancing the anti-fatigue and yang-tonifying ability of sub-health young and middle-aged men, as well as conditioning middle-aged and elderly patients with physical deficiency.

The invention belongs to the technical field of sperm cell treatment, and specifically relates to a method for isolating and identifying sperm cells of Buffalo testis, including the following steps: obtaining Buffalo testis, digest with collagenase IV, hyaluronidase and DNase I combined enzyme, sieving, filtering, and taking suspension cells after differential adherence; 2) taking the suspend cells for live cell stain; and 3) separating by flow cytometry. Beneficial effect: the technical scheme is an effective method for separating buffalo sperm cells, By digesting bovine testis tissue with two enzymes, so that a single cell suspension is obtain, the interference of somatic cells is removed by the method of continuous differential adherence, according to the fluorescence intensity of non-adherent cells stained by flow cytometry, the DNA content of cells is judged, and the interference of other non-target cells such as diploid spermatogonia and tetraploid primary meningoblasts is effectively digested and removed to achieve the successful sorting of a large number of haploid spermatozoa.

The invention relates to a new medical use of astaxanthin, i.e. prevention and treatment of organophosphorus pesticide induced male reproductive toxicity, and further relates to application in drugs inhibiting organophosphorus pesticide induced sperm motility decrease, sperm abnormality rate increase, testis tissue MDA content increase, and testis tissue CAT activity and LDH activity decrease. High-toxic organophosphorus pesticide and low toxicity organophosphorus pesticide phoxim are adopted as the representative to study the prevention and treatment effect of astaxanthin on male reproductive toxicity caused by low concentration organophosphorus pesticide exposure, and the study proves that astaxanthin can inhibit male reproductive toxicity induced by organophosphorus pesticide and has good dose dependence, thus putting forward application of astaxanthin as an active material in preparation of drugs for organophosphorus pesticide induced male reproductive toxicity.

The invention relates to the technical field of biology, in particular to establishment and induced differentiation methods of a spermatogonial stem cell line of opsariichthys bidens. The method comprises the following steps: (1) separating and digesting testis tissues of opsariichthys bidens, and performing planking culture on cells; (2) after the cells are adhered to the wall, carrying out passage, and continuously performing culturing for 45-60 generations. The spermatogonial stem cells of the opsariichthys bidens obtained by the method can be continuously passaged in vitro and can be differentiated into somatic cells of the opsariichthys bidens through induction, such as neuronal cells, epithelial cells, huge flat cells and astrocytes; and the cells can be induced and differentiated into sperms.

The invention provides a takifugu flavidus protamine extraction method. Extraction process conditions include that pH of 0.14mol/L of NaCl solution is 8.5, the concentration of sulfuric acid solutionis 0.6mol/L, consumption of the sulfuric acid solution is 4.0 volumetric times of a precipitant, an extraction temperature is 45 DEG C, extraction time is 2.4h, consumption of ethyl alcohol is 4 volumetric times of supernatant, and the actual extraction rate of protamine is 19.07%. Extracting protamine from takifugu flavidus testes provides an experimental foundation for research on physicochemical properties and related functional characteristics of protamine, and a function of effective utilization of takifugu flavidus testis tissues can be achieved.

The embodiment of the invention discloses an extraction method of globefish testis milt components. The extraction method of the globefish testis milt components includes the steps: globefish testes are pretreated, and scattered testis cells are obtained; ultrasonication is conducted on the scattered testis cells, and filtration and separation are carried out to obtain white precipitate; a set volume of concentrated hydrochloric acid is added to dissolve the white precipitate, and then a solution is formed; the solution passes through an ultrafiltration membrane, and then a trapped liquid anda filtrate are obtained separately; extraction is conducted on the trapped liquid, and then milt nucleic acids are obtained; and extraction is conducted on the filtrate, and then milt proteins are obtained. According to the extraction method of the globefish testis milt components, by utilizing the globefish testes as an extraction raw material, a large quantity of globefish viscus wastes producedby a globefish culture industry can be effectively utilized. Besides, according to the technical scheme, separation and extraction of the milt DNA and the milt proteins are achieved, thus, the availability of testis tissue can be improved, usage of surface active agents and organic solvents is also reduced, and therefore the extraction method of the globefish testis milt components is more environmentally friendly.

The invention provides application of geniposide in preparation of a medicine for treating asthenospermia. Experimental results show that the geniposide can significantly improve the sperm quantity and activity and reduce the sperm malformation rate, can significantly inhibit endoplasmic reticulum stress inside testis, increase mitochondrial membrane potential, regulate expression of related proteins in testis tissues, and improve excessive apoptosis of spermatogenic cells.

The invention discloses an establishment method for differentiating swine germline stem cells into sperms in vitro. The establishment method includes the steps of subjecting piglet testis tissues to isolation culture to obtain primary piglet testis tissue fibroblasts and germline stem cells, culturing the testis tissue fibroblasts for 15 days by means of a Niche culture medium for inducing in-vitro differentiation of sperms to obtain a Niche feeder layer for inducing in-vitro differentiation of sperms, and inoculating the germline stem cells to the Niche feeder layer to culture for 27-30 days, thereby obtaining haploid sperm-like cells in vitro by cytobiology analysis. The establishment method is easy to operate and good in repeatability, is an important breakthrough in research on swine mGSCs(male germline stem cells) in-vitro differentiation, provides an important basic technology for researches on swine embryonic in-vitro operation and transgenic breeding, and meanwhile, has high reference value in research on in-vitro differentiation of sperms of other species and assisted reproduction of human beings.

The invention belongs to the technical field of medical equipment, and particularly relates to a highly-integrated and intelligent testis paracentesis cooperating platform which comprises a cart frameand an operating table device. The cart frame comprises a cart body, wheels and a collecting box, the wheels are arranged at the bottom of the cart body, and the collecting box is arranged on the cart body; the operating table device comprises a table body, telescopic rods, a stand column, a table lamp, a tray and a surgery device, the table body is supported by the cart body through the four telescopic rods, the stand column is arranged on the edge of the left side of the table body, the table lamp is arranged at the top end of the stand column, the tray is arranged on the right side of thetable top of the table body, and the surgery device comprises a testis tissue separating device and a testis tissue taking-out device, wherein the testis tissue separating device is arranged on the front edge of the table body, and the testis tissue taking-out device is arranged on the rear edge of the table body. The highly-integrated and intelligent testis paracentesis cooperating platform has rapidness, convenience and flexibility in testis paracentesis of medical staff.

The present invention discloses Henan Huaxi crab (Sinopotamon henanense) reproductive marker gene vasa and its application, the nucleotide sequence of the gene is shown in the sequence table SEQ ID NO.1; also discloses the protein encoded by Henan Huaxi crab gene vasa Sequence, as shown in the sequence table SEQ ID NO.2, and the physical and chemical properties of the protein, the characteristics of amino acid residues, and the characteristics of the protein structure and functional domains. The coding region of the gene starts at the 142nd nucleotide at the 5' end and ends at the 2214th nucleotide, encoding 690 amino acid residues. The reproductive marker gene of Henan Huaxi crab of the present invention is specifically expressed in the ovary and testis tissue of Henan Huaxi crab through identification, and can be used for the development and application of reproductive marker molecules of Henan Huaxi crab and its similar species, and can be used to guide river crab Artificial breeding has practical significance.

The invention provides a novel application of plant fruit pulp. The invention relates to application of plant fruit pulp in testis function protection, application of plant fruit pulp in prevention and/or treatment of oligospermia and asthenospermia, application of plant fruit pulp in protection of male fertility and application of plant fruit pulp in prevention and/or treatment of testis tissue lesion, testosterone level decline, testis function decline, sperm number reduction, asthenospermia and asthenospermia caused by metabolic disorder. The sperm motility rate is reduced; the sperm morphology is abnormal. The plant fruit pulp can effectively prevent and treat symptoms such as testicular tissue lesion, testosterone level decline, testicular hypofunction, sperm number reduction, sperm motility rate reduction and sperm morphology abnormality caused by metabolic disorder. The application provides a basis for protecting testis functions, preventing and/or treating oligospermia and asthenospermia and protecting male fertility by directly utilizing the plant fruit pulp.

The present invention discloses application of compound 6-furfuryl amino purine in preparing medicine for treating testis senility. It is proved by means of experiment that compound 6-furfuryl amino purine as one kind of exogenic purine matter can raise the antioxidase activity of the testis tissue of senile mouse, raise the ability of body's eliminating free radical, reduce the damage of cell caused by lipoid preoxidation, raise the antioxidant capacity of mouse's testis and raise the serum testosterone level of senile mouse, improve sex gland function and delay senility of sex gland.

The invention provides a molecular breeding method based on the polymorphism of a candidate gene PRL (Prolactin) and relates to the technical field of poultry breading, in particular to a method for utilizing the polymorphism of the candidate gene PRL to perform molecular breeding. Under the condition of focusing on a gene effect of a PRL gene in a gonadal axis of a chicken, i.e. hypothalamus-hypophysis-ovary or testis tissue to reproductive characters of the chicken, mRNA differential expression or difference of translating proteins thereof, factors influencing the expression of the reproductive characters of the chicken are researched and a genetic molecular mechanism of the reproductive characters of the chicken is primarily illustrated, so that a function of the candidate gene during a reproductive developing process can be objectively understood, and meanwhile, the analysis to the influence thereof on a reproductive performance can be facilitated, a basis for marking auxiliary breeding can be provided, the improvement of the reproductive characters can be quickened and the potential economic benefit of the candidate gene can be utilized.

A method for detecting the nucleoproteins of spermatogenic cells through utilizing indirect immunofluorescent staining comprises the following steps: separating convoluted seminiferous tubules from the testis tissues of an animal, utilizing a hypotonic solution to process spermatogenic cells, and detecting the nucleoproteins of the spermatogenic cells through adopting indirect immunofluorescent staining, wherein the hypotonic solution comprises 17mM of sodium citrate, 50mM of sucrose, 5mM of EDTA, 0.5mM of DTT, 0.5mM of PMSF and 30mM of Tris and having a pH value of 8.2. The method uses the hypotonic solution to carry out permeable treatment of the cells in order to rapidly expand the cells, disperse chromatins and fully release the proteins in cell nuclei, so the spermatogenic cells can well combine with primary antibodies, thereby the combination efficiencies of secondary antibodies are enhanced, fluorescent staining is complete and full, and the preparation of spermatogenic cell chromosomes and karyotyping are successfully carried out.

Cancer-testis (CT) antigens have been identified by screening public databases for transcripts that are expressed in tumor tissues and a limited set of normal tissues, or by screening for genes that are expressed in cancer and testis tissues (but not other normal tissues). The invention relates to nucleic acids and encoded polypeptides which are CT antigens expressed in patients afflicted with cancer. The invention provides, inter alia, isolated nucleic acid molecules, expression vectors containing those molecules and host cells transfected with those molecules. The invention also provides isolated proteins and peptides, antibodies to those proteins and peptides and cytotoxic T lymphocytes which recognize the proteins and peptides. Fragments of the foregoing including functional fragments and variants also are provided. Kits containing the foregoing molecules additionally are provided. The molecules provided by the invention can be used in the diagnosis, monitoring, research, or treatment of conditions characterized by the expression of one or more CT antigens.

The invention belongs to the technical field of medical detection, and discloses a specific marker screening method for detecting azoospermia and severe oligospermia. The method comprises the following steps: collecting a sample, treating the sample, and screening out specific markers of the severe oligospermia and azoospermia; in a testicular tissue level, screening expression of differential microRNA in testicular tissues of normal males and sterile males by using a chip method; in a seminal plasma layer, searching differential marker proteins of normal males and sterile males by using a proteomics method; in the urine level, employing metabonomics for screening the difference of metabolites in urine of normal men and infertility men; integrating data obtained from three high-flux layers, namely a testis tissue layer, a seminal plasma layer and a urine layer, and carrying out correlation analysis on the data and sperm quality, density and vitality; and optimizing and establishing a diagnosis technology. The method is long in detection time, high in efficiency and capable of explaining the cause of dyszoospermia of the patient.

The invention discloses a method for identifying the physiological sex of fish at an early development stage by using 5S rRNA, which comprises the following steps: (1) extracting the total RNA of fish gonad tissues by using a method for conventionally extracting the total RNA of animal cells by using a total RNA extraction kit or a TRIzol reagent, determining an OD260/280 value by using a nucleic acid analyzer, and judging the RNA purity, and then separating total RNA by using an agarose gel electrophoresis method, and observing the brightness and distribution of 5S rRNA, 18S rRNA and 28S rRNA; (2) carrying out microcapillary electrophoresis on the extracted gonad total RNA, and calculating the proportion of 5S rRNA in the total RNA according to the RNA type coverage area obtained by nucleic acid analysis software; (3) according to the proportion of the 5S rRNA in the total RNA, judging the physiological male and female of the fish at the early development stage, when the proportion of the 5S rRNA is smaller than or equal to 9.0%, judging the fish as a testis tissue, and when the proportion of the 5S rRNA is larger than or equal to 12%, judging the fish as an ovarian tissue. The method is easy to implement and simple and convenient to operate, the physiological sex identification of more than 100 fish individuals is realized within 12 hours, and the physiological sex of the fish is determined.

The invention discloses an in-vitro separation method for primary spermatogenic cells of rats. The method comprises the following steps: 1) collecting the testis on two sides of a fresh in-vitro rat, and stripping envelopes and blood vessels to obtain testis tissues; 2) digesting the testicular tissue to prepare a single-cell suspension; 3) culturing the single-cell suspension until non-spermatogenic cells are adhered to the wall, sucking out non-adhered suspension cells, and separating the suspension cells by adopting a Percoll method to obtain the spermatogenic cells. According to the method, a foundation is laid for in-vitro research on the reproductive function of the rat testis, in-vitro separation and culture of the rat testis spermatogenic cells are achieved, and it is proved that the spermatogenic cells separated and cultured through the method are high in in-vitro survival rate and long in survival time. Normal rat testicular spermatogenic cells can be obtained by using the method, the cost is low, the operation is simple, the repeatability is high, various experimental requirements can be met, and the method is worthy of large-scale popularization.

The invention is suitable for the technical field of biological cryopreservation, and provides a bovine testis tissue serum-free cryopreservation protective agent and a cryopreservation method and application thereof. The serum-free cryopreservation protective agent comprises the following components: a Knockout DMEM medium, a KSR serum substitute, dimethyl sulfoxide and mycillin. According to theinvention, the Knockout DMEM culture medium which does not contain serum is used as a substrate, the kSR serum substitute, dimethyl sulfoxide and mycillin are used as low-temperature protective agents in a combined manner, the cost of the cryopreservation protective agent can be reduced, the risk of exogenous pathogen and virus pollution is avoided, in addition, the bovine testis tissue can be preserved at low temperature for a long time, cell apoptosis is reduced, the tissue form is maintained, spermatogenic cells and testis cells are well protected, the testis tissue after cryopreservationand resuscitation is complete in structure, the activity of various testis cells is high, and normal proliferation and development can be achieved.

The invention relates to the application of retinoic acid in preparation of drugs for improving prognosis of cryptorchidism, and belongs to the technical field of medicine. The drug is mainly composedof retinoic acid, which can improve the infertility of cryptorchidism patients, and can improve the spermatogenesis function of cryptorchidism patients by improving the oxidative stress level and apoptosis level of cryptorchidism patients' testis tissue and the expression level of blood-testis barrier-associated protein. This provides a basis for improving the clinical treatment strategy for cryptorchidism and improving the prognosis of cryptorchidism.