56 results about "Testis tissue" patented technology

The invention discloses an application of hydrogen sulfide and donor thereof in improving the sperm quality and function, and relates to the field of reproduction. The hydrogen sulfide can be directly added into semen in vitro, and the activity of the sperms of clinical asthenospermia patients can be remarkably improved within short time. The hydrogen sulfide also can resist oxidative stress for the sperms cultured in vitro so as to reduce the stress injury of the sperms caused by manual operation and in-vitro culture and maintain the activity level of the sperms. The hydrogen sulfide also can be used for improving the dyszoospermia caused by infection, protecting the spermatogenic structure of testis, maintaining the completion of blood-testis barrier and the synthesis of androgen, reducing the inflammatory level in the testis tissue and the oxidative stress of spermatogenic cells, and reducing the apoptosis of spermatogenic cells caused by infection so as to maintain a normal spermatogenic process, keep the sperm quality and maintain normal fertility of male. The invention opens up a new application field of hydrogen sulfide and provides a significant reference to solving the human dysgenesis and improving the assisted reproductive technology.

The method of separating and purifying chicken's spermatospore relates to trnasgene, rare species preservation, tissue and cell engineering, and other fields. The present invention includes first separating chicken's spermatospore and subsequent Percoll separation and adherent purification. The present invention provides proper chicken's spermatospore separating and purifying method by means of separating chicken's spermatospore from chicken's testis tissue, and Percoll gradient and adherent purification, spermatogenic epithelium cell suspension with great testis cell number and high survival rate of testis cell is prepared while ensuring elimination of other somatic cell components.

The invention discloses a preparation method of a slide for specific spermatogenic cells at a development stage, and the method comprises the following steps: removing the envelope of a testis tissue which is taken from an animal, dispersing contorted senimiferous tubules, determining staging of a part corresponding to a spermatogenic epithelium wave mode according to the light transmission difference of the contorted senimiferous tubules under a dissecting microscope, then selecting an interesting part section to prepare the slide, and fixing the slides by 1wt% of paraformaldehyde solution after being frozen in liquid nitrogen, thus preparing the required slide for the specific spermatogenic cells. The method has the maximum advantage that the living cellular level of related molecules in the spermatogenic process is detected in vitro, complete, objective and precise experimental results can be obtained, and the results reflect expression features of a specific gene product of the spermatogenic cells at the development stage.