Preparation method of slide for specific spermatogenic cells at development stage

Abstract

The invention discloses a preparation method of a slide for specific spermatogenic cells at a development stage, and the method comprises the following steps: removing the envelope of a testis tissue which is taken from an animal, dispersing contorted senimiferous tubules, determining staging of a part corresponding to a spermatogenic epithelium wave mode according to the light transmission difference of the contorted senimiferous tubules under a dissecting microscope, then selecting an interesting part section to prepare the slide, and fixing the slides by 1wt% of paraformaldehyde solution after being frozen in liquid nitrogen, thus preparing the required slide for the specific spermatogenic cells. The method has the maximum advantage that the living cellular level of related molecules in the spermatogenic process is detected in vitro, complete, objective and precise experimental results can be obtained, and the results reflect expression features of a specific gene product of the spermatogenic cells at the development stage.

Description

Technical field [0001] The invention relates to a method for preparing a cell slide, in particular to a method for preparing a spermatogenic cell slide with specific stages of male mammal spermatogenesis. Background technique [0002] Due to the decline in the quantity and quality of male sperm, a large number of infertile couples have appeared, which has negatively affected the society in many aspects. At present, the incidence of infertility is as high as 15% to 20%, and the male factor accounts for about half. Male infertility is the main problem of male reproductive health, which is related to the overall quality of the people and the healthy development of the population in the future. It has become a focus issue that urgently needs social attention and urgently need to be resolved. Carrying out basic research on male reproductive health and revealing the mechanism of spermatogenesis can not only provide laboratory evidence for elucidating the pathogenesis of male infertili...

AI Technical Summary

Problems solved by technology

Although this type of method can ensure the uniformity of the specificity of the developmental stages of germ cells, it needs to prepare the tissue into a cell suspension first, which not only destroys the inherent structure and shape of the testicular tissue and seminiferous tubules, but also cannot accurately distinguish the various stages of all developmental stages. spermatogenic cells at all levels, thereby missing some biological information

[0007] Various existing methods of film production require the use of chemical reagents to fix tissues and cells before slicing or spreading, which inevitably results in the loss of intracellular information

Up to now, there is still a lack of an effective in vitro culture system for spermatogenic cells, and it is difficult to analyze the spermatogenic process and reproductive regulation-related genes at the cellular level at the living cell level