Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

DNA molecular marker method for identification of alien invasive species Spartina alterniflora population

A technology of DNA molecules, Spartina alterniflora, applied in the field of DNA molecular markers to identify invasive alien species Spartina alterniflora populations, achieving high success rate, good reproducibility, and genetic stability

Inactive Publication Date: 2014-08-20
NANJING UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, most of the researches are mainly focused on the ecological processes such as competitive exclusion, niche replacement, biological characteristics, allelopathy, predation, parasitism, etc., and lack of population dynamics, rapid adaptation and evolutionary models, rapid invasion and expansion dynamics, etc. Understanding of intrusion mechanisms

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • DNA molecular marker method for identification of alien invasive species Spartina alterniflora population
  • DNA molecular marker method for identification of alien invasive species Spartina alterniflora population
  • DNA molecular marker method for identification of alien invasive species Spartina alterniflora population

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0022] The present invention will be further described in detail below in conjunction with specific samples, which are explanations of the present invention rather than limitations.

[0023] 1. Population survey and sampling

[0024] Spartina alterniflora in Luoyuan Bay, Fujian Province, the earliest area where invasive alien species were introduced, is the most primitive population in my country, and it was used as a sampling point. Chongming Island in Shanghai is close to the analysis point, and the samples are highly preserved after collection, so it is used as the sampling point.

[0025] Select leaves that grow well and have no pests, and immediately put them into a pre-prepared ziplock bag filled with about half a bag of silica gel after picking, and mix well to fully mix the leaves and silica gel for rapid dehydration. After taking it back to the laboratory, replace the discolored silica gel according to the actual situation, and after drying, it will be used for DNA e...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a DNA molecular marker method for identifying alien invasive species Spartina alterniflora populations. This method obtains target genes based on high-throughput transcriptome sequencing screening, and obtains multiple single nucleotide polymorphisms through PCR reactions and PCR product sequencing. sex (SNP) loci. All valid single nucleotide polymorphism sites are then used to form a haplotype for molecular markers to achieve genetic typing. The present invention can obtain rich polymorphism information in one experiment. Single nucleotide polymorphism markers are genetically stable, can be used for samples from different sources, and are suitable for high-throughput automated analysis.

Description

technical field [0001] The invention belongs to the technical field of ecological engineering, and relates to a DNA molecular marker method for identifying populations of alien invasive species Spartina alterniflora. Background technique [0002] Alien biological invasion has become one of the most urgent problems in environmental protection today, and it has a very important impact on the economy, ecology, social security and international trade of all countries in the world. The U.S. spends $137 billion a year directly or indirectly on invasive species management. China has also become one of the countries that suffer the most serious damage from the invasion of alien species in the world. 488 species of alien invasive species have been discovered, of which more than 50 species rank among the world's 100 most threatened alien species announced by the International Union for Conservation of Nature. The direct economic loss amounted to 120 billion yuan, and the investment i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCY02A90/40
Inventor 陈建群许颖王强丁静吴娟子
Owner NANJING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com