Breeding method for amphiprotic fertile autotetraploid crucian carps, establishment method for strains of crucian carps and breeding method for triploid fishes

An autotetraploid and allotetraploid technology is applied in fish farming, application, climate change adaptation, etc. It can solve problems such as unstable fertility and failure to form tetraploid fish strains, and achieve high Hatch rate, obvious economic and social benefits, and the effect of fast growth

Active Publication Date: 2014-06-25
湖南岳麓山水产育种科技有限公司
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Problems solved by technology

In fish genetics and breeding, methods such as cold / heat shock can usually be used to inhibit the first mitosis of fertilized eggs to prepare autotetraploid fish, but tetraploid fish lines cannot be formed due to unstable fertility

Method used

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  • Breeding method for amphiprotic fertile autotetraploid crucian carps, establishment method for strains of crucian carps and breeding method for triploid fishes
  • Breeding method for amphiprotic fertile autotetraploid crucian carps, establishment method for strains of crucian carps and breeding method for triploid fishes
  • Breeding method for amphiprotic fertile autotetraploid crucian carps, establishment method for strains of crucian carps and breeding method for triploid fishes

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Embodiment

[0034] A method for establishing a hermaphrodite autotetraploid crucian carp strain of the present invention comprises the following steps:

[0035] First, in the crossbreeding of crucian carp F 1 The allotetraploid crucian carp was selected from the offspring and raised for more than two years. During the breeding season, female individuals capable of producing eggs of different sizes and male individuals capable of producing watery semen were selected from the allotetraploid population of crucian carp as the maternal parent fish and the male parent fish for artificial induction, and then artificial dry insemination , put the fertilized eggs in a petri dish for running water incubation, and then raise the hatched fry, and select the sexually fertile autotetraploid crucian carp from the self-bred offspring of the reared allotetraploid crucian carp Breeding the autotetraploid crucian carp obtained by breeding for 1 year, and selecting female and male individuals with better go...

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Abstract

The invention discloses a breeding method for amphiprotic fertile autotetraploid crucian carps and an establishment method for strains of the crucian carps. The breeding method includes the following steps that allotetraploid crucian carps are selected from crucian carp hybridization F1 offspring with red crucian carps as female parents and megalobrama amblycephala as male parents, female individuals capable of generating ova of different sizes and male individuals capable of generating watery semen are selected from the allotetraploid crucian carps for hasten parturition, insemination is carried out manually through a dry method, running water incubation is conducted on fertilized ova, then the fertilized ova are bred, and the amphiprotic fertile autotetraploid crucian carps are selected from selfing offspring. Selfing is carried out on the male individuals and the female individuals in the obtained autotetraploid crucian carps, and the strains of the amphiprotic fertile autotetraploid crucian carps can be established and obtained through multi-offspring breeding. Ploid hybridization is conducted on the autotetraploid crucian carp colonies obtained through the breeding method and diploid crucian carps, so allotriploid fishes can be bred on a large scale. The strains of the crucian carps obtained through the breeding method are excellent in character, stable in heredity and high in fertilization rate and hatchability, and the breeding method has a great significance in genetic research of fishes.

Description

technical field [0001] The invention relates to a method for fish breeding and establishment of fish strains, in particular to a method for breeding sexually fertile autotetraploid fish from allotetraploid crucian carp. Background technique [0002] Red crucian carp belongs to Cyprinidae, Cyprinidae, and Cyprinus biologically. Crucian carp has high nutritional value and rich protein content, and has always been a favorite fish for consumers. The sterility of triploid fish shows significant advantages in terms of growth rate, stress resistance, disease resistance and meat quality. And the establishment of genetically stable tetraploid fish strains can be used to produce 100% sterile triploid fish on a large scale by intercrossing with diploid fish. [0003] Distant hybridization (that is, hybridization between species or above) is an important fish genetic breeding and trait improvement method, which can integrate a whole set of exogenous genes, thereby changing the genetic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 刘少军覃钦博刘筠罗凯坤张纯陶敏肖军
Owner 湖南岳麓山水产育种科技有限公司
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