The invention discloses an automatic and integrated
system for extracting, amplifying and detecting nucleic acids. The
system is matched with a disposable and enclosed double face disc type reaction box of a prepackaged
reagent, in order to realize automatic
nucleic acid detection with 'a sample enters and a result outputs'. The
system contains a driving control carrying platform for loading, rotating and exerting the double-face disc type reaction box from the bottom, and a top control component for exerting the same reaction box from the top. The driving control carrying platform and the topcontrol component are provided with an execution device for vertically acted on the double-face disc type reaction box, the execution device provides experimental operations, such as fixation and rotary positioning of the reaction box, unidirectional or reciprocating rotation of the reaction box, high speed
centrifugation for driving fluid to flow, opening or enclosing of a sample
injection port,a valve and a channel and other switches or seal membranes on the reaction box, heating of the prepackaged
reagent and adsorption of magnetic beads, multizone
temperature control and multipath
fluorescence detection, and the like; automatic and integrated extraction, amplification and detection of
nucleic acid in the enclosed reaction box are realized.