The invention relates to a hand-care disposable disinfection gel and a preparation method thereof. The hand-care disposable disinfection gel comprises ethanol, a bactericide, a cosolvent, a thickener, an emollient, essence and a PH regulator, wherein the essence is used as a carrier to cover pungent odor of ethanol. The hand-care disposable disinfection gel has the advantages of being bactericidal, washing-free, not sticky and greasy, and comfortable in smell, and caring hands. The preparation method comprises the following steps: the thickener swells in purified water fully to prepare a liquid A; meanwhile, the bactericide and the cosolvent are added in ethanol to be mixed uniformly to prepare a liquid B; then, the liquid B is poured into the liquid A; the emollient and the essence are poured in the liquid A, after purified water is supplemented to be sufficient, the PH regulator is added to regulate the PH value to 6.0-8.0, and the uniform mixing is performed.