Molecular breeding method based on polymorphism of candidate gene PRL (Prolactin)
A candidate gene and molecular breeding technology, applied in the field of chicken breeding, can solve the problems of low egg production and qualified egg rate, and achieve the effect of accelerating improvement
Inactive Publication Date: 2012-05-23
YANGZHOU JINMIAO AGRI TECH
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Problems solved by technology
The amount of high-quality broiler chickens in my country is increasing at a rate of 10%-20% every year. However, the shortcomings of local chicken breeds in my
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[0016] Marker-assisted breeding was carried out according to the gene effect of the PRL gene mRNA sequence in the chicken gonadal axis on GenBank, that is, the hypothalamus-pituitary-ovary or testis tissue, on chicken reproductive traits, mRNA differential expression and translation protein differences.
[0017] A RT-PCR method for gene expression analysis was established.
[0018] The mutation of PRL gene in local chicken breeds was detected by sequencing typing method.
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Abstract
The invention provides a molecular breeding method based on the polymorphism of a candidate gene PRL (Prolactin) and relates to the technical field of poultry breading, in particular to a method for utilizing the polymorphism of the candidate gene PRL to perform molecular breeding. Under the condition of focusing on a gene effect of a PRL gene in a gonadal axis of a chicken, i.e. hypothalamus-hypophysis-ovary or testis tissue to reproductive characters of the chicken, mRNA differential expression or difference of translating proteins thereof, factors influencing the expression of the reproductive characters of the chicken are researched and a genetic molecular mechanism of the reproductive characters of the chicken is primarily illustrated, so that a function of the candidate gene during a reproductive developing process can be objectively understood, and meanwhile, the analysis to the influence thereof on a reproductive performance can be facilitated, a basis for marking auxiliary breeding can be provided, the improvement of the reproductive characters can be quickened and the potential economic benefit of the candidate gene can be utilized.
Description
technical field [0001] The invention relates to the technical field of chicken breeding, in particular to a method for molecular breeding using PRL candidate gene polymorphism. Background technique [0002] There are many varieties of local chicken in China, and the meat quality and flavor are excellent, which is recognized by the world. The production of high-quality broiler chickens with local chicken breeds as the main breeding materials or direct provenance has become a new economic growth point of my country's animal husbandry. The amount of high-quality broiler chickens in my country is increasing at a rate of 10%-20% every year. However, the shortcomings of local chicken breeds in my country such as low egg production and qualified eggs have become a bottleneck restricting the development of high-quality broiler industry to high efficiency. In many places in China, the annual egg production of high-quality chicken breeds is about 100, and the lowest is only about 70....
Claims
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IPC IPC(8): C12Q1/68A01K67/027
Inventor 刘玉清刘胜军
Owner YANGZHOU JINMIAO AGRI TECH
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