39 results about "Genetic molecular" patented technology

The invention provides an SNP molecular marker related to egg weight and application of the SNP molecular marker. The SNP molecular marker corresponds to 169, 930, 974th deoxynucleotide of a Number one chromosome positive-sense strand of chicken reference genome Gallus_gallus-4.0 version sequence information issued in NCBI, wherein the nucleotide is T or G. An SNP locus is never reported before, thereby being a newly-found genetic molecular marker. The SNP molecular marker can be applied to early-stage selection of egg weight at different week ages and assisting of chicken breeding, lowering of production cost and accelerating of genetic progress can be facilitated, and the SNP molecular marker has quite high economic application value and scientific research value.

The invention relates to a method for obtaining an SSR (Simple Sequence Repeat) primer of lycium ruthenicum based on transcription sequencing, and belongs to the technical field of molecular marking techniques of molecular genetics of biology. The method comprises the following steps of (1), carrying out transcriptome sequencing; (2), screening an EST-SSR (Expressed Sequence Tag-Simple Sequence Repeat) site; (3), carrying out primer design; (4), extracting DNA (Deoxyribonucleic Acid); (5), carrying out primer screening; (6), carrying out polymorphic primer screening by using 8-percent native polyacrylamide gel electrophoresis. The method for obtaining the SSR primer of the lycium ruthenicum based on the transcription sequencing, which is provided by the invention, can be used as a method for the correction of a linkage map of a genome between remote species and comparative mapping, and has higher utilization value in two aspects. The greatest advantages of the method are that the development is simple and quick and the expense is low. The general characteristic of the SSR of the lycium ruthenicum is specified by utilizing an SSR molecular marking technique; the SSR primer of the lycium ruthenicum is developed; the foundation is laid for carrying out the research on the genetic diversity, the linkage map construction and the affinity relationship of species resources of the lycium ruthenicum by utilizing an SSR molecular marker, so that the method is better applied to molecular breeding.

The invention discloses a marker for detecting osteoarthritis. The marker is GALNT18, IGFLR1 and MYDGF proteins. Further, the invention also provides application of the marker for detecting osteoarthritis to preparation of a reagent, a kit or a chip for diagnosing osteoarthritis. The three kinds of proteins with difference expression in the urine of a patient with the osteoarthritis are discovered; in addition, the application prospects in the early stage detection of the osteoarthritis are studied; the urine proteins relevant to the osteoarthritis provided by the invention can be used as early stage bio-marker for the osteoarthritis; a novel direction is provided for further studying the genetic molecular mechanism of osteoarthritis and exploring the medicine target points for osteoarthritis early stage prevention and treatment.

The invention discloses a genetic molecular marker co-segregated with a male sterility gene msc-2 of a capsicum annuum cell nucleus and an application of the genetic molecular marker. Primers for detecting capsicum annuum fertility are provided and are a primer pair capable of amplifying a specific fragment; the specific fragment comprises a target sequence of the primer pair consisting of a primer F and a primer R; the primer F is following a1) or a2): a1) single stranded DNA molecules represented as a sequence 1 of a sequence table; a2) DNA molecules which are obtained by substitution and/ordeletion and/or addition of the sequence 1 by one or more nucleotides and have the same function with the sequence 1; the primer R is following a3) or a4): a3), single stranded DNA molecules represented as a sequence 2 of the sequence table; a2) DNA molecules which are obtained by substitution and/or deletion and/or addition of the sequence 2 by one or more nucleotides and have the same functionwith the sequence 2. Fertile plants can be rapidly identified in the seedling stage according to the developed marker.

The invention relates to application of an LY6G5C gene as a molecular marker to preparation of a reagent for diagnosis of exposure to heavy ion radiation, belonging to the field of application of genetic molecular markers. As the LY6G5C gene is used as the molecular marker for detection of exposure to heavy ion radiation, detection is more convenient and accurate and a radiation dose can be determined.

The invention discloses a pair of primers for screening cows with mastitis resistance. The sequence of an upstream primer is shown in SEQ ID NO.7 and the sequence of a downstream primer is shown in SEQ ID NO.8. The invention also discloses a kit for screening cows with mastitis resistance. The kit comprises the primers and conventional reagents required for PCR (polymerase chain reaction) amplification. The primers and the kit can be used for screening cows with mastitis resistance. An application method comprises the steps of extracting genome DNA (deoxyribonucleic acid) of cows to be detected, carrying out specific PCR amplification by utilizing the primers, and detecting the PCR amplification products to judge the genotype, wherein the hybrid type has relatively strong mastitis resistance, therefore, the cow individuals with hybrid genotypes are selected for genetic molecular breeding of cows. The method has the effects of improving the accuracy of selection of cow individuals with mastitis resistance and saving the genetic improvement time of mastitis resistance of cows, and has good application prospects.

The invention relates to application of an ABCG1 gene as a molecular marker to preparation of a reagent for diagnosis of exposure to heavy ion radiation, belonging to the field of application of genetic molecular markers. As the ABCG1 gene is used as the molecular marker for detection of exposure to heavy ion radiation, detection is more convenient and accurate and a radiation dose can be determined.

The invention discloses a genetic molecular marking combination for anti-disease stichopus japonicas breeding and application of the genetic molecular marking combination and belongs to the field of aquatic product genetic breeding. The genetic molecular marking combination consists of three SNP (Single Nucleotide Polymorphism) markers SNP88, SNP112 and SNP126 related to anti-vibrio splendidus infection of stichopus japonicas, and the three marked dominant genotypes are respectively SNP88 (CC), SNP112 (AA) and SNP126 (TT). A breeding method with the genetic markers comprises the following operation steps: (1) extracting parent DNA (Deoxyribonucleic Acid) and typing; (2) selecting parents; (3) performing filial generation seedling breeding. By adopting the genetic molecular marking combination, effective molecular markers for improving the economic property of anti-vibrio splendidus infection of stichopus japonicas are provided, the genetic markers are adopted for auxiliary marker selection of stichopus japonicas, anti-disease breeding can be carried out, the anti-vibrio splendidus infection of the stichopus japonicas can be improved, the morbidity of skin ulcer syndromes of the stichopus japonicas can be substantially reduced, and the combination has great significances in increasing the breeding survival rate and the breeding benefits.

The present invention relates to the technical field of molecular markers and particularly to a genetic molecular marker related to pork quality conductivity traits. The molecular marker can be used to breed pigs with higher pork conductivity. A breeding method is as follows: conducting sampling, extracting DNA and performing quality testing, performing genotyping to obtain SNP typing data, and obtaining the molecular marker related to the pork meat conductivity. A nucleotide sequence of the SNP molecular marker is shown in SEQ ID NO:1, an A/C allelic mutation exists at a 51st base of the sequence, the nucleotide of the shown sequence produces polymorphism, and when the 51st nucleotide in the nucleotide sequence is C, pork has higher conductivity and better quality.

The invention provides a molecular breeding method based on the polymorphism of a candidate gene PRL (Prolactin) and relates to the technical field of poultry breading, in particular to a method for utilizing the polymorphism of the candidate gene PRL to perform molecular breeding. Under the condition of focusing on a gene effect of a PRL gene in a gonadal axis of a chicken, i.e. hypothalamus-hypophysis-ovary or testis tissue to reproductive characters of the chicken, mRNA differential expression or difference of translating proteins thereof, factors influencing the expression of the reproductive characters of the chicken are researched and a genetic molecular mechanism of the reproductive characters of the chicken is primarily illustrated, so that a function of the candidate gene during a reproductive developing process can be objectively understood, and meanwhile, the analysis to the influence thereof on a reproductive performance can be facilitated, a basis for marking auxiliary breeding can be provided, the improvement of the reproductive characters can be quickened and the potential economic benefit of the candidate gene can be utilized.

The invention discloses a group of biomarkers for assessing the aortic dissection risks and application of the biomarkers. The biomarkers can be one or a combination of several of gene mutation sitesin Table 1. The invention further provides application of the gene mutation sites to preparation of detection products for detecting or assessing the aortic dissection disease. The invention finds 44aortic dissection susceptible mutation sites and studies application prospects thereof in early prediction of aortic dissection; aortic dissection-related gene sites provided by the invention can be used as early biomarkers for the aortic dissection; a new direction is provided for further studying the genetic molecular mechanism of the aortic dissection and exploring medicine targets for early prevention and treatment of the aortic dissection.

The invention discloses the application of a SNP (single nucleotide polymorphism) locus in the preparation of an early detection product for obesity and/or hypertriglyceridemia in Chinese children. The SNP locus is rs189326455, specifically chr1: 233463936, c. 162G) C, p. Glu54Asp. The invention studies the gene locus rs189326455 associated to obesity as an early biomarker of obesity and hypertriglyceridemia and provides a new direction to further study the genetic molecular mechanism of obesity and hypertriglyceridemia and explore the drug targets for early prevention and treatment of obesityand hypertriglyceridemia.

The invention belongs to the technical field of molecular biology and biomedicine and discloses an endometrial cancer susceptibility associated genetic molecular marker detection kit. A nucleotide sequence of a genetic molecular marker is shown as SEQ ID NO.1, and single nucleotide polymorphism C>T exists at 501th site, namely a basic group at the 501th site of the sequence can be a basic group Cand a basic group T. The kit comprises two pairs of specific PCR primers designed on the basis of the single nucleotide polymorphism genetic molecular marker of an NANOG gene, the genetic molecular marker is closely associated with endometrial cancer susceptibility, single nucleotide polymorphism in the NANOG gene can be effectively detected by means of the PCR technology, and further, endometrialcancer susceptibility can be predicted, so that important clinical significance to screening of endometrial cancer susceptible population, detection of high-risk individuals and early prediction, early diagnosis and individual treatment of the endometrial cancer is achieved.

The invention relates to a single nucleotide polymorphism sites related to obesity of Chinese children and application thereof. The invention discloses a biomarker related to obesity of Chinese children. The biomarker comprises one or two SNP sites of susceptibility to obesity of Chinese children, wherein the sites comprises susceptibility SNP site (1): rs1059491, or in concrete, the chr16: 28603655, c. 704A> C, p. N235T; susceptibility SNP site (2): rs768847893, or in concrete, the chrX: 3241682, c. 2044> T, p. R682C. According to the single nucleotide polymorphism sites related to obesity of Chinese children, two susceptibility SNP sites are found, and the application prospect of the sites in the auxiliary tests of children obesity is investigated. The gene sites related with obesity are possibly the early biomarkers of obesity, and provide a new direction for the further investigation of the genetic molecular mechanism of obesity and the exploration of drug targets for the early prevention of obesity.

The invention belongs to the technical field of gene engineering, and discloses a genetic molecular marker related to gastric cancer auxiliary diagnosis and an application thereof and totally 42 genetic loci are provided. According to the invention, a genetic risk prediction model is constructed. The invention provides an application of specific primers of the SNP marker and an application of thespecific primers in preparation of a gastric cancer auxiliary diagnosis kit, and the gastric cancer auxiliary diagnosis kit. The genetic marker kit for assisting in gastric cancer diagnosis is expected to lay a foundation for screening and individualized prevention and treatment of high-risk groups of gastric cancer in China, and a decision basis is provided. The SNP genetic marker is different from a traditional biomarker, and is good in stability and easy to detect. The gastric cancer auxiliary diagnosis kit is helpful for clinicians to quickly grasp the genetic risk of patients suffering from gastric cancer, perform early diagnosis and individualized prevention and treatment, and provide a decision basis for screening and intervention of high-risk groups of gastric cancer.