39 results about "Genetic molecular" patented technology

A method for detecting interactions between first and second interacting molecules a variable sensitivity. This variable sensitivity may be obtained by providing for the overexpression of either a bait hybrid protein containing a DNA binding domain (desensitization) or a prey hybrid protein containing the DNA activation domain for a reporter gene (enhanced sensitivity). The use of exogenous activators of one or the other according to the needs of a particular system is readily accomplished.

The invention discloses a genetic molecular marking combination for anti-disease stichopus japonicas breeding and application of the genetic molecular marking combination and belongs to the field of aquatic product genetic breeding. The genetic molecular marking combination consists of three SNP (Single Nucleotide Polymorphism) markers SNP88, SNP112 and SNP126 related to anti-vibrio splendidus infection of stichopus japonicas, and the three marked dominant genotypes are respectively SNP88 (CC), SNP112 (AA) and SNP126 (TT). A breeding method with the genetic markers comprises the following operation steps: (1) extracting parent DNA (Deoxyribonucleic Acid) and typing; (2) selecting parents; (3) performing filial generation seedling breeding. By adopting the genetic molecular marking combination, effective molecular markers for improving the economic property of anti-vibrio splendidus infection of stichopus japonicas are provided, the genetic markers are adopted for auxiliary marker selection of stichopus japonicas, anti-disease breeding can be carried out, the anti-vibrio splendidus infection of the stichopus japonicas can be improved, the morbidity of skin ulcer syndromes of the stichopus japonicas can be substantially reduced, and the combination has great significances in increasing the breeding survival rate and the breeding benefits.

The invention discloses a pair of primers for screening cows with mastitis resistance. The sequence of an upstream primer is shown in SEQ ID NO.7 and the sequence of a downstream primer is shown in SEQ ID NO.8. The invention also discloses a kit for screening cows with mastitis resistance. The kit comprises the primers and conventional reagents required for PCR (polymerase chain reaction) amplification. The primers and the kit can be used for screening cows with mastitis resistance. An application method comprises the steps of extracting genome DNA (deoxyribonucleic acid) of cows to be detected, carrying out specific PCR amplification by utilizing the primers, and detecting the PCR amplification products to judge the genotype, wherein the hybrid type has relatively strong mastitis resistance, therefore, the cow individuals with hybrid genotypes are selected for genetic molecular breeding of cows. The method has the effects of improving the accuracy of selection of cow individuals with mastitis resistance and saving the genetic improvement time of mastitis resistance of cows, and has good application prospects.

The invention discloses a genetic molecular mark for fast-growing stichopus japonicus breeding and application thereof, and belongs to the field of aquatic product genetic breeding. The molecular markis an SNP160 mark; the amplification molecular mark is an SNP160 primer pair: SNP160F: 5'-TCGCAGGAAACCAACCTTCA-3' and SNP160R:5'-AAGGGAGGGTCAAGGAGGAT-3'. The mark is used for fast growing the property auxiliary breeding advantage genotype of AA. Meanwhile, the invention also discloses the auxiliary breeding steps of the mark. The effective molecular mark is provided for the economic property of the stichopus japonicus growth in the genetic aspect; when the genetic mark is used for mark auxiliary selection and developing the genetic breeding, the growth speed of seedlings can be effectively accelerated; important significance is realized on improving the culture benefits.

The invention belongs to the technical field of molecular biology and biomedicine and discloses an endometrial cancer susceptibility associated genetic molecular marker detection kit. A nucleotide sequence of a genetic molecular marker is shown as SEQ ID NO.1, and single nucleotide polymorphism C>T exists at 501th site, namely a basic group at the 501th site of the sequence can be a basic group Cand a basic group T. The kit comprises two pairs of specific PCR primers designed on the basis of the single nucleotide polymorphism genetic molecular marker of an NANOG gene, the genetic molecular marker is closely associated with endometrial cancer susceptibility, single nucleotide polymorphism in the NANOG gene can be effectively detected by means of the PCR technology, and further, endometrialcancer susceptibility can be predicted, so that important clinical significance to screening of endometrial cancer susceptible population, detection of high-risk individuals and early prediction, early diagnosis and individual treatment of the endometrial cancer is achieved.

The invention discloses the application of SNP sites in the preparation of early detection products for Chinese children's obesity and / or hypertriglyceridemia. The SNP sites are rs189326455, specifically chr1:233463936, c.162G>C, p.Glu54Asp . The present invention studies the genetic locus rs189326455 related to obesity as an early biomarker of obesity and hypertriglyceridemia, in order to further study the genetic molecular mechanism of obesity and hypertriglyceridemia, and to explore the early prevention and treatment of obesity and hypertriglyceridemia The drug target provides a new direction.

The present invention discloses a method for screening SNP sites related to aortic dissection disease based on whole exome sequencing technology. The SNP sites include one or two of the following aortic dissection susceptibility SNP sites: PTK6_c. 205G>A_p.E69K; TSHZ2_c.724C>T_p.R242C. Further, the present invention provides the application of the SNP site in a detection product for detecting aortic dissection disease. The present invention discovers two aortic dissection susceptible SNP loci and studies their application prospects in early prediction of aortic dissection, and the aortic dissection-related gene loci provided by the present invention may be early biomarkers of aortic dissection It provides a new direction for further studying the genetic molecular mechanism of aortic dissection and exploring drug targets for early prevention and treatment of aortic dissection.