21 results about "Genetic molecular" patented technology

The invention discloses an HNRNPA2B1 gene-based bovine sperm malformation rate related SNP molecular marker and application thereof, and belongs to the technical field of genetic molecular biology, the number of the SNP molecular markers is two, SNP1 is at the position of a bovine genome chr4: 69733358, the polymorphism is C or A, SNP2 is at the position of a bovine genome chr4: 69733387, and the polymorphism is T or G. According to the two SNP loci provided by the invention, the dominant genotypes of g.-1713A > C and g.-1684G > T are AA and GG respectively, and the sperm collection quantity and the sperm malformation rate of the two SNP loci have better performance. By detecting the polymorphism of the two SNP loci, the motility evaluation of the bull sperms can be realized, the breeding is assisted, a reliable support is provided for breeding a high-quality next generation, and the method has the advantages of high reliability, high efficiency, low cost and the like.

The invention belongs to the technical field of biomedicine, and discloses an immortalized cell line for human kidney suspected cell carcinoma as well as a culture method and application of the immortalized cell line. The immortalized cell line for the human kidney suspected cell carcinoma, disclosed by the invention, is named as a human kidney suspected cell carcinoma cell line Loya a-710 (Homo sapiens), the classification name of the Latin name of the immortalized cell line is Chromophore sensor cell carcino: Loya a-710, and the preservation number of the immortalized cell line is CCTCC (China Center for Type Culture Collection) NO: C2025123. The human kidney suspected cell carcinoma cell line Loya-710 disclosed by the invention is used as an in-vitro model to rapidly grow in tissue culture, and mitochondrial mutation characteristics, a vesicle structure and a classical immunohistochemical marker of ChRCC are reserved. The invention provides a precious tool for further research on ChRCC heredity, molecules and biological characteristics, and also provides a powerful new model for mitochondrial diseases.

The invention relates to a Yangtze river globefish polymorphic SNP molecular marker as well as a development method and application thereof, and belongs to the technical field of DNA molecular markers, nucleotide sequences of sites of the Yangtze river globefish polymorphic SNP molecular marker are as shown in SEQ ID NO: 1-NO.19, primers for amplifying the sites of the SNP molecular marker are as shown in Snpp 01-57, and the primers for amplifying the sites of the SNP molecular marker are as shown in SEQ ID NO: 1-SEQ ID NO: 2-SEQ ID NO: 3-SEQ ID NO: 4. The SNP molecular markers provided by the invention are all derived from high-quality chromosome-level genomes of the Yangtze-River cowfish, screening is carried out in combination with re-sequencing data of three geographical populations, polymorphic SNP loci in the genomes of the Yangtze-River cowfish can be more comprehensively detected, and by utilizing a KASP genotyping technology, the SNP molecular markers can be used for detecting the genomes of the Yangtze-River cowfish. Genetic typing can be efficiently and accurately performed on the polymorphic SNP sites of the Yangtze river globefish, the detection cost is remarkably reduced, and an effective genetic molecular marker is provided for population genetics research such as genetic diversity evaluation, genetic differentiation analysis and genetic relationship identification of the Yangtze river globefish and the sibling species of the Yangtze river globefish.

The invention discloses a bull semen quality related SNP molecular marker based on a CCDC89 gene and application of the bull semen quality related SNP molecular marker, and belongs to the technical field of genetic molecular biology, the SNP molecular marker is located at the position of chr29: 9868636 of the bull CCDC89 gene, the gene polymorphism is G or A, and when the SNP site is G, high semen quality is shown. By detecting the polymorphism of the SNP site, the detection of the bull semen quality and the identification of the reproductive performance can be realized, the breeding is assisted, the reliable support is provided for breeding the next generation of high quality, and the SNP site has the advantages of high reliability, high efficiency, low cost and the like.

U biosensors, and related U-sensing genetic molecular components, genetic circuits, compositions, methods and systems are described, which in several embodiments can be used to detect and / or neutralize uraniunm and in particular bioavailable U.

The application discloses a SNP site, a CAPS molecular marker and application which are closely linked to a wheat salt stress post-maximum relative root length character, and belongs to the technical field of wheat genetic molecular breeding.The SNP site is at least one of the 274th, 689th, 725th, 767th, 811th and 1038th SNP sites of a TaHSFA2-2A gene sequence.The six SNPs exist in two haplotypes, namely haplotype Hap-2A-1 and haplotype Hap-2A-2, and the two haplotypes of the six SNPs are linked.Through correlation analysis and natural population verification, the maximum relative root length of wheat germination stage salt stress of Hap-2A-2 is significantly greater than that of Hap-2A-1.Therefore, the application has important application value in predicting the maximum relative root length of wheat salt stress or in preparing a reagent for predicting the maximum relative root length of wheat salt stress.

The present invention belongs to the field of biomedical technology and discloses an immortalized cell line of human renal chromophobe carcinoma, a culture method and an application thereof. The immortalized cell line of human renal chromophobe carcinoma of the present invention is named human renal chromophobe carcinoma cell line Loya-710 (Homo sapiens), and its Latin name is Chromophobe renal cell carcinoma:Loya‑ 710 , deposited with CCTCC NO: C2025123. The human chromophobe renal cell carcinoma cell line Loya-710, as an in vitro model, grows rapidly in tissue culture and retains the mitochondrial mutations, vesicle structure, and classic immunohistochemical markers characteristic of ChRCC. This invention provides a valuable tool for further studying the genetic, molecular, and biological characteristics of ChRCC and offers a powerful new model for mitochondrial disease.

The invention discloses a genetic molecular marker related to colorectal cancer auxiliary diagnosis and application of the genetic molecular marker, and belongs to the field of genetic engineering and oncology. 37 SNPs significantly related to the colorectal cancer onset risk are screened out, the colorectal cancer auxiliary diagnosis kit prepared based on 37 markers is helpful for clinicians to rapidly master the genetic risk of colorectal cancer onset, powerful support is provided for early prevention measures, and the colorectal cancer auxiliary diagnosis kit is suitable for multiple populations and has a wide application prospect. The problem that a conventional genetic risk prediction model is not compatible with people in China and Europe and America is effectively solved.

The invention discloses a colorectal cancer related genetic molecular marker with an interaction effect with drinking and application of the colorectal cancer related genetic molecular marker. The genetic molecular marker is a combination of rs61355123, rs17624213, rs3791337, rs190489984 and rs2290476. According to the method, the limitation that a traditional genetic model only pays attention to the genetic main effect is broken through, and the multi-gene risk scoring model integrating environmental factors is constructed by recognizing a group of key SNP loci having remarkable interaction with drinking behaviors. The gene-environment collaborative assessment mechanism realizes more accurate risk stratification for drinkers. Clinical test data show that compared with a traditional genetic model, the genetic score of the integrated genetic marker provided by the invention has the following advantages: (1) the prediction accuracy is improved; (2) the clinical practical value is prominent; and (3) the intervention pertinence is enhanced.

The invention belongs to the technical field of animal husbandry genetic breeding, and particularly relates to a mitochondrial SNP molecular marker related to egg laying performance of laying hens and application of the mitochondrial SNP molecular marker. The invention provides a mitochondrial SNP (Single Nucleotide Polymorphism) molecular marker related to the egg laying performance of laying hens, which is positioned at the 15944th bp of a chicken mitochondrial genome and has C / A / T polymorphism. The mitochondrial genome has maternal hereditary characteristics, systematic mitochondrial whole genome hereditary variation analysis is carried out to verify the genetic effect of the laying hen breeding trait mitochondrial gene, and the poultry genetic breeding theory is supplemented and perfected; moreover, a novel extranuclear genetic molecular marker and a variety breeding method are established, and the method has practical application value and important scientific significance.

The present invention relates to a composition for detecting a target gene based on cDNA synthesis using a ligation method that does not use reverse transcription and a method for multiple ligation-assisted recombinase polymerase amplification, and since a target gene may be detected through a visual change with only a short reaction time of about 30 minutes at room temperature without the synthesis of cDNA using reverse transcriptase, the present invention may be effectively used for point-of-care genetic molecular diagnosis of RNA viruses and the like.

The present application belongs to the field of animal models, and more specifically, a method for constructing an autism spectrum disorder mouse model by BAF155 conditional knockout mode is disclosed. By constructing a specific knockout BAF155 mouse, the effect of knockout BAF155 on OL development and myelination is observed. Further proved by the behavior experiment, the deletion of BAF155 in oligodendrocyte precursor cells will lead to the appearance of the core and characteristic ASD-like behavior phenotype of mice mainly with social preference disorder and repetitive stereotyped behavior. Therefore, the present application successfully constructs a new genetic molecular defect ASD animal model, and the advantage lies in that the target is clear, a single genetic molecular defect can be induced to study ASD, and a suitable and matched animal model for ASD caused by mutation of BAF155 is provided, which is helpful for precise diagnosis and subsequent intervention strategy.

This invention relates to a molecular marker for polymorphic SNPs in the Yangtze finless porpoise, its development method, and its application. Belonging to the field of DNA molecular marker technology, the nucleotide sequences of the polymorphic SNP molecular marker sites in the Yangtze finless porpoise are shown in SEQ ID NO:1-NO.19, and the primers used to amplify the SNP molecular marker sites are shown in Snpp 01-57. The SNP molecular markers provided by this invention are all derived from high-quality chromosome-level genomes of the Yangtze finless porpoise and are screened using resequencing data from three geographical populations. This allows for a more comprehensive detection of polymorphic SNP sites in the Yangtze finless porpoise genome. By utilizing KASP genotyping technology, genotyping of polymorphic SNP sites in the Yangtze finless porpoise can be performed efficiently and accurately, significantly reducing detection costs. This provides effective genetic molecular markers for population genetics studies such as genetic diversity assessment, genetic differentiation analysis, and kinship identification of the Yangtze finless porpoise and its closely related species.

The present invention discloses a bovine sperm deformity rate-associated SNP molecular marker based on the HNRNPA2B1 gene and its application, belonging to the field of genetic molecular biology technology. There are two SNP molecular markers: SNP1 is located at the cattle genome chr4:69733358 position, with a polymorphism of C or A; SNP2 is located at the cattle genome chr4:69733387 position, with a polymorphism of T or G. The two SNP sites provided by the present invention, g.-1713A>C and g.-1684G>T, have dominant genotypes of AA and GG, respectively, and their semen collection volume and sperm deformity rate have better performance. By detecting the polymorphisms of these two SNP sites, bull sperm motility assessment can be achieved, thereby assisting breeding and providing reliable support for breeding high-quality next-generation breeding, with advantages such as high reliability, high efficiency, and low cost.

UO2F2 biosensors, and related U-sensing and / or F-sensing genetic molecular components, genetic circuits, compositions, methods and systems are described, which in several embodiments can be used to detect and / or neutralize uranium and in particular bioavailable UO2F2.

The invention discloses an SNP site closely linked with the maximum relative root length character of wheat after salt stress, a CAPS molecular marker and application, and belongs to the technical field of wheat genetic molecular breeding. The SNP (Single Nucleotide Polymorphism) site is at least one of the 274th site, the 689th site, the 725th site, the 767th site, the 811th site and the 1038th site of a TaHSFA2-2A gene sequence. The six SNPs have two haplotypes, namely, the haplotype Hap-2A-1 and the haplotype Hap-2A-2, and the two haplotypes of the six SNPs are linked with each other. Correlation analysis proves and natural population verifies that the maximum relative root length of Hap-2A-2 after salt stress in the wheat germination period is obviously larger than that of Hap-2A-1. Therefore, the method has important application value in predicting the maximum relative root length of the wheat after salt stress or preparing a reagent for predicting the maximum relative root length of the wheat after salt stress.

The invention discloses a molecular marker related to eggshell strength in the egg laying peak period and application of the molecular marker, and belongs to the technical field of biology. The nucleotide sequence of the molecular marker is as shown in SEQ ID NO. 1; t / C mutation exists at the 200th basic group of the sequence as shown in SEQ ID NO. 1. According to the invention, an SNP (Single Nucleotide Polymorphism) genetic molecular marker (chr7: 14542996, rs313628382, GRCg6a) highly related to the strength of the egg shell is screened out. The eggshell strengths of individuals with different genotypes at the site are obviously different. Based on the molecular marker, a specific primer is developed, a blood DNA detection method is established, the method can identify individuals with high eggshell strength characters in the early stage only by collecting a small amount of blood, the method has the advantages of convenience, high flux and labor saving, and the breeding cost can be greatly saved.

To provide a new therapeutic strategy for dealing with fundamental genetic / molecular defects of hereditary pulmonary disease patients.SOLUTION: Provided are recombinant nucleic acids comprising one or more polynucleotides encoding a polypeptide (e.g., an inhaled therapeutic polypeptide, such as a human alpha-1-antitrypsin polypeptide); viruses comprising the recombinant nucleic acids; compositions and formulations comprising the recombinant nucleic acids and / or viruses; methods of use thereof (e.g., to deliver a polypeptide to one or more cells of the respiratory tract and / or for the treatment of a disease affecting the lungs, such as alpha-1-antitrypsin deficiency); and articles of manufacture or kits thereof.SELECTED DRAWING: Figure 3

The application relates to the technical field of water environment monitoring, and discloses a method for estimating the nucleotide metabolism function intensity of water bodies based on the optical characteristics of DOM (dissolved organic matter), which is specifically as follows: based on the optical characteristics of the dissolved organic matter (DOM), the optical parameters and the functional gene relative abundance data of the modeling samples are acquired, the gene abundance data is subjected to central logarithmic ratio transformation, a multiple linear regression model is constructed, and model verification and evaluation are completed, and finally, the optimal prediction model for rapidly estimating the relative abundance of the key functional genes of the water bodies is obtained. The application solves the defects of the traditional detection methods, such as complicated operation, high cost, low space-time resolution and the like, realizes rapid, non-invasive and in-situ estimation of the nucleotide metabolism and genetic molecular information functional genes of the water bodies, greatly shortens the detection period, and is suitable for large-scale water environment monitoring, water body ecological health evaluation, pollution early warning and carbon cycle process research.

The present application relates to the field of genetic molecular breeding, and discloses a black sheep 40K liquid chip based on single nucleotide polymorphism and application thereof. The preparation method of the chip is as follows: phenotype data of Turpan black sheep is obtained, DNA of collected blood is extracted and sequenced; after quality control of original data obtained by sequencing of the DNA library, the original data is compared with a sheep reference genome, sequencing is performed after comparison, and BAM data is obtained; variation detection is performed on BAM data of all samples, all SNP sites are obtained and quality controlled; after quality control of the SNP data, chip background SNP site screening and breed-specific SNP site screening are performed; the selected chip background SNP sites and breed-specific SNP sites are combined, and a probe is designed to prepare a liquid chip. The chip can maximize the use of upstream and downstream SNP marker information of target sites, thereby improving breeding accuracy and efficiency and promoting the directional improvement of production performance of Turpan black sheep.

The present invention discloses a bull semen quality-related SNP molecular marker based on the CCDC89 gene and its application, belonging to the field of genetic molecular biology technology. The SNP molecular marker is located at the chr29:9868636 position of the bovine CCDC89 gene, and the gene polymorphism is G or A. When the SNP site is G, high semen quality is exhibited. By detecting the polymorphism of this SNP site, the bull semen quality can be tested and the reproductive performance can be identified, thereby assisting breeding and providing reliable support for breeding high-quality next generations. The method has the advantages of high reliability, high efficiency, and low cost.