20 results about "Semen quality" patented technology

The invention discloses a method for predicting the sperm motility of a breeding bull based on a host SNP-rumen microorganism-metabolite interaction axis, and the sperm motility of the breeding bull can be obtained by detecting the haplotype condition of SNP1-SNP3 and / or SNP4-SNP6 sites of a breeding bull genome. The method breaks through the limitations of strong dependency on phenotype endpoint evaluation and prediction lag in the current bull seed selection process, and realizes forward accurate prediction and early evaluation of the semen quality of the breeding bull.

The present application belongs to the technical field of bull semen quality evaluation, and particularly relates to a local bull semen quality evaluation method and system based on multi-source data analysis. The method comprises: when real-time data corresponding to a current semen sample is acquired, executing a quality evaluation process to generate a comprehensive score of the current semen sample; based on the offset amplitude of sample data generated by the evaluation model in the benchmark test, determining a main parameter and at least one subsidiary parameter from a plurality of to-be-tested parameters associated with the current semen sample. The present application calculates a subsidiary score according to the subsidiary parameter, and adjusts the subsidiary score by using an external parameter to generate a comprehensive score; and a multi-dimensional dynamic evaluation framework is constructed to consider a plurality of to-be-tested parameters of the semen itself, and the external parameter is quantified and integrated into the final score, so that the evaluation result can dynamically adapt to different environments and individual conditions, and overcome the limitation of single evaluation dimension in the prior art.

The application provides a pig semen quality rapid analysis instrument and relates to the field of reproductive experiment test platforms. The pig semen quality rapid analysis instrument comprises a workbench, the one side of the tabletop of the workbench is provided with an up-down through-type placing groove, comprises an electric sliding component, is installed on the placing groove and can independently slide forward and backward, comprises a constant-temperature storage component, the electric sliding component can be linked to open the constant-temperature storage component when moving forward, comprises a convenient cleaning and sterilization component, is fixedly installed on the electric sliding component. The pig semen quality rapid analysis instrument can spray and disinfect the handheld sampling equipment, can perform immersion cleaning when heavy cleaning, both forms are relatively fast, especially the hand flushing disinfection and drying for reuse, improve the reuse efficiency of the semen sampling analysis instrument, and manual use of alcohol is not needed, the operator's hand does not leave the equipment all the time, the probability that the equipment is contaminated is greatly reduced, and therefore the experimental analysis result obtained is more accurate.

The invention belongs to the technical field of animal breeding, and particularly relates to application of alpha-ketoglutaric acid or salt thereof in preparation of a sheep semen cryopreservation diluent. The invention discloses the application of alpha-ketoglutaric acid or salt thereof in the cryopreservation of the sheep semen for the first time, and the alpha-ketoglutaric acid or salt thereof can improve the semen quality of the frozen and unfrozen sheep semen and improve the fertilization ability of the frozen semen; such as sperm motility, sperm movement performance, sperm viability, sperm plasma membrane integrity rate, sperm acrosome integrity rate, sperm antioxidant capacity, sperm ATP level, sperm early apoptosis incidence rate, sperm in-vitro fertilized ovulation rate and / or artificial fertilization pregnancy rate, can be used for preparing the sheep sperm cryopreservation diluent.

The invention discloses application of pseudomonas as a marker in evaluation of bull semen quality, and belongs to the technical field of animal breeding and microbiomics. The relative abundance of Pseudomonas in a bull rumen fluid with high semen quality is significantly higher than the relative abundance in a bull rumen fluid with low semen quality. By detecting the relative abundance, the bull semen quality can be evaluated, and the reproductive performance can be reliably judged on the premise of not depending on a semen sample. Evaluation can be carried out before semen collection for the first time or in a breeding candidate period, potential low-fecundity individuals are screened out in advance, and the breeding risk is reduced.

The invention provides application of exogenous D-ribose in improving the normal-temperature preservation effect of boar semen and a method for improving the normal-temperature preservation effect of the boar semen. According to the technology disclosed by the invention, D-ribose is added into the boar semen stored at normal temperature, so that the effects of enhancing the oxidation resistance of the semen, improving the sperm motility and improving the quality of the semen can be achieved, and particularly, indexes such as the sperm motility, the plasma membrane integrity rate, T-AOC, CAT, GSH-Px and SOD are obviously higher than those of all groups including a control group; the ROS content of the sperms is obviously lower than that of all other groups.

The invention belongs to the technical field of livestock breeding, and discloses composite insect powder, composite insect powder zymolyte and a feed additive for improving the quality of breeding male poultry semen. The composite insect powder is tenebrio molitor powder, hermetia illucens powder and cricket powder. Furthermore, the composite insect powder is adopted for enzymolysis, and the effect after enzymolysis is better. After the composite insect powder and the composite insect powder zymolyte are used as feed additives to be added into breeding male poultry feed for feeding, core reproductive performance indexes such as sperm motility and sperm density can be remarkably improved, the sperm motility can reach 86% or above, the sperm density can reach 4 * 10 < 9 > / mL or above, and the breeding male poultry can be fed with the composite insect powder zymolyte. And the compound insect powder has obvious multiple compound effects of yellow mealworms, hermetia illucens and crickets. The compound insect powder is used as a feed additive, the breeding male poultry semen quality is comprehensively improved through multi-dimensional indexes, the raw materials are easy to obtain, use is easy and convenient, and the compound insect powder feed is especially suitable for popularization of large-scale breeding poultry breeding.

The invention discloses a method for improving inhibition of slow heat stress on male rabbit breeding, which is executed by a computer system and comprises the following steps: acquiring environmental data and physiological indexes such as male rabbit ear temperature and respiratory rate in real time through a multi-modal sensor; the core algorithm constructs a slow heat stress cumulative damage model containing a time decay factor, and quantifies the integral effect of the heat load and the body repair process. The system generates multi-dimensional optimal control vectors of environment cooling, nutrition intervention and LED red light repair through a physiology-environment coordinated regulation and control model on the basis of an accumulated damage index generated by the model. According to the method, the limitation of traditional single threshold value control is broken through, and full-period precise prevention and control of physical cooling open circuit, nutrition restoration follow-up and illumination rehabilitation ending is achieved. Experiments prove that the scheme can effectively protect testis tissues, shorten the reproductive function recovery period and improve the semen quality, the breeding and medicament cost is reduced while the reproductive performance is guaranteed, and the method is suitable for different scales of rabbit farms.

The invention relates to the technical field of livestock breeding, in particular to a special lick brick for relieving heat stress and improving bull semen quality as well as a preparation method and application of the special lick brick. According to the special lick brick, on the premise that basic mineral supply is not changed, through synergistic addition of astaxanthin and betaine, key semen quality indexes such as the number of sperms, sperm motility and the like of Holstein breeding bulls advancing linearly are pertinently improved. Meanwhile, through the synergistic effect of astaxanthin and betaine, the oxidation resistance of the body can be remarkably enhanced, the heat stress related index level is reduced, and inhibition of heat stress on reproductive performance is relieved. The preparation method provided by the invention is simple and convenient in process, convenient to use and controllable in cost, not only can meet the requirement of large-scale breeding for practicability, but also can indirectly improve the breeding economic benefit by improving the semen quality, is suitable for daily breeding of 1-4-year-old Holstein breeding bulls, and has wide popularization and application prospects.

The invention discloses application of methyl nicotinate as a marker in evaluation of bull semen quality, and belongs to the technical field of animal reproduction and metabonomics. The relative content of methyl nicotinate in the bull rumen fluid with high semen quality is significantly higher than the relative content of methyl nicotinate in the bull rumen fluid with low semen quality. By detecting the relative content, the bull semen quality is evaluated, the reproductive performance can be reliably judged on the premise of not depending on a semen sample, and the method has the remarkable advantages of being non-invasive, high in throughput, capable of advancing a prediction window and the like.

The utility model relates to the technical field of semen quality analysis, in particular to a concentric circle sperm quality analyzer sample pretreatment structure which comprises a monitor, the bottom end of the inner side of the monitor is fixedly connected with a stepping motor, and the output end of the stepping motor is fixedly connected with a connecting disc. A first circular ring, a second circular ring and a third circular ring are fixedly connected to the top of the connecting disc, an insertion hole is formed in the first circular ring, a sampling cup is slidably connected to the insertion hole, a fixing cylinder is fixedly connected to the bottom end of the inner side of the insertion hole, and placement grooves are symmetrically formed in the outer side of the fixing cylinder; the concentric circle sperm quality analyzer sample pretreatment structure comprises a fixing cylinder, a placing groove is formed in the fixing cylinder, a mounting disc is fixedly connected to the inner side of the fixing cylinder, a connecting groove is formed in the mounting disc, and the interior of the placing groove is communicated with the interior of the connecting groove. And sampling cups with different sizes can be quickly clamped and fixed.

The utility model relates to a sperm freezing descent control device which comprises a liquid nitrogen tank used for containing liquid nitrogen, a pedestal and a controller, hollow horizontal rods are arranged on the pedestal, a winding wheel is rotatably connected between the inner side walls of the left ends of the horizontal rods, a fixed pulley is rotatably connected between the inner side walls of the right ends of the horizontal rods, and the fixed pulley is connected with the controller. A pull rope is wound on the winding wheel, and the free end of the pull rope naturally droops after bypassing the fixed pulley and is detachably connected with a hanging basket used for containing a freezing thin tube; a motor electrically connected with the controller is arranged at the left end of the horizontal rod, the output end of the motor is fixedly connected with the winding wheel, a distance sensor used for monitoring the height between the hanging basket and the liquid level of liquid nitrogen in the liquid nitrogen tank is arranged on the hanging basket, and the distance sensor is in signal connection with the controller. Different liquid nitrogen gas phase pre-freezing heights can be adjusted according to different semen qualities, so that the semen is ensured to be at a proper pre-freezing temperature, the semen freezing quality is improved, and the inactivation rate is reduced.

The application relates to the technical field of semen quality evaluation, and discloses a semen quality evaluation method based on deep learning, which comprises the following operation steps: S1, pre-processing a data set, selecting data of 85 male participants aged 18 or above, the data containing a video of a live sperm sample of each participant, using 60% of the data set for training, using 20% as a verification set, using 20% as a test set, pre-processing the training set video, and taking 9 continuous frames as a sample input. The semen quality evaluation method based on deep learning directly extracts video information of the semen video by using a light-weight MobileNet, improves the convolutional neural network, adds other data in addition to the video information, increases the prediction accuracy of the network, deploys the neural network by using a front-end and back-end separated Web end, solves the prediction efficiency problem, and makes the whole system better in operation and better in displaying the prediction result.

The invention discloses a diluent for normal-temperature preservation of boar semen and a preservation method. Selenomethionine is selected as a protective agent in the diluent for preserving the boar semen at the normal temperature to improve the semen quality, the diluent for preserving the semen at the low temperature is a Modena basic diluent, and the addition amount of the selenomethionine in the diluent for preserving the semen at the normal temperature is 0.1-10 mg / L, preferably 1 mg / L. According to the invention, selenomethionine is added into the diluent for preserving the boar semen at normal temperature, so that the motility, motility rate, plasma membrane integrity rate and acrosome integrity rate of the boar semen can be remarkably improved. If the method is applied to actual production, the normal-temperature preservation time of the boar semen can be prolonged, the semen preservation quality is improved, and the production cost is reduced.

The invention relates to the technical field of biological breeding, and discloses an application of a PDHB gene inhibitor in improving the preservation quality of frozen pig sperms.By means of the technology, exogenous miR-let-7a or mimics or analogues for inhibiting PDHB gene expression are added into frozen and preserved pig semen, so that the effects of improving the sperm motility and improving the semen quality are achieved; especially, indexes such as sperm motility, sperm mitochondrial ROS content, membrane potential and the like are obviously superior to those of all groups including a control group.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for influencing the linear motion / far-end mass droplet character of a pig sperm. The SNP molecular marker is Agt located at the 31908556 bp position of the 10th chromosome of a pig genome Ensembl Sscrofa11.1 version; g mutation. By verifying the influence effect of the molecular marker on linear motion and far-end mass droplet characters of pig sperms, an efficient and accurate molecular marker assisted breeding technology is finally established, and the molecular marker assisted breeding technology is applied to genetic improvement of boars for improving the quality of the boar sperms, so that the reproductive performance of offspring pigs is improved, the economic profit of enterprises is increased, and the core competitiveness is increased. By optimizing the dominant allele A of the SNP, the frequency of the dominant allele A can be increased generation by generation, the semen quality of the boars can be improved, excellent boars with the two characters can be bred cooperatively, the genetic improvement progress of the boars can be accelerated, and the economic benefit of boar breeding can be effectively improved.

The invention relates to the technical field of biological breeding, and discloses an application of exogenous miR-92a or a simulant or an analogue in improving the preservation quality of fresh boar sperms.The exogenous miR-92a or the simulant or the analogue is added into the fresh boar sperm preserved at normal temperature through the technology, PI3K / AKT signal channels are activated by down-regulating expression of PTEN, and the preservation quality of the fresh boar sperm is improved. The effects of improving the sperm motility, improving the sperm plasma membrane intact rate and improving the semen quality can be achieved.

The invention relates to the technical field of poultry genetic breeding, and particularly discloses a breeding method for improving the continuous fertilization ability of chickens. The core of the method comprises the following steps: constructing a hen individualized physiological baseline and a cock semen quality baseline; dynamically evaluating the reproductive steady state of the hens in the laying period, regulating and controlling the microenvironment as required, and synchronously updating the seminal fluid quality of the cocks; determining a hybridization window period based on the fertilization potential index, and combining historical data to intelligently match and hybridize; early evaluating the fertilization ability of the candidate offspring by using the prediction model, and screening and establishing a new generation of breeding group; key data is extracted every day to generate a data block hash evidence so as to guarantee traceability. The method can be used for precise breeding of good chicken varieties, and has the advantages of adapting to individual physiological differences, improving mating and breeding efficiency and guaranteeing credibility of breeding data; meanwhile, the standardization degree of the breeding process is high, and breed improvement and upgrading of the large-scale chicken raising industry can be remarkably promoted.

The invention discloses an intestinal bacterium for improving semen quality and application thereof, and belongs to the field of microbial technology and biological medicine. In order to solve the problem that in the prior art, specific probiotics are not used for improving male reproductive injury induced by high fat diet, the invention provides a method for applying the allobacter faecalis strain (A.s) or an inactivated form (P-A.s) of the allobacter faecalis strain (A.s) to the male reproductive injury. Animal experiments show that compared with a high fat diet model group, supplementation of A.s or P-A.s can significantly inhibit abnormal weight growth, improve testis index, improve sperm density, vitality, movement speed and testicular testosterone level, and can effectively repair tissue form damage of testis and epididymis and abnormal sperm ultrastructure. Wherein the improvement effect of the inactivated bacterium form P-A.s is particularly prominent.

The invention belongs to the technical field of molecular biology, discloses a ZNF354A gene-based male donkey semen quality character molecular marker and application thereof, and further finds out the molecular marker significantly related to the male donkey semen quality character by analyzing the ZNF354A gene polymorphism and the correlation between the ZNF354A gene polymorphism and the male donkey semen quality character. The genotype of the exon 4SNP (G530A) of the ZNF354A gene is obviously related to the quantity of the collected blood; in the aspect of sampling quantity, the AA genotype is obviously higher than GA and GG, and the GA and the GG have no obvious difference. The ZNF354A gene exon 4 region SNP (G530A) can be used as a potential molecular genetic marker for male donkey semen quality character breeding, is applied to male donkey specialized strain breeding, and accelerates the breeding process.