59 results about "Semen quality" patented technology

The invention discloses an automatic grading and grouping system and method for boars, and relates to the technical field of boar breeding, and the system constructs a multi-modal biological characteristic data set by collecting gene, semen quality, health examination and growth performance data. Secondly, extracting gene features by using a convolutional neural network, calculating a gene screening coefficient JYX, and classifying the gene screening coefficient JYX into an A class level and a B class level through a first threshold Q1; then, semen quality analysis is conducted on the A-class boars, a semen quality screening coefficient ZLX is calculated, and the A-class boars are divided into the A1-class boars and the A2-class boars through a second threshold Q2; and when the A2-class boar triggers health early warning, further evaluating a health index JCZ, and subdividing the health index JCZ into A21, A22 and A23 classes according to a third threshold Q3. And finally, grouping the boars according to the class levels of the boars, and formulating an accurate feeding strategy to optimize the reproductive performance and improve the breeding benefits.

The invention belongs to the technical field of breeding equipment, and particularly discloses a boar semen quality analysis method, device, equipment and medium, and the method comprises the following steps: 1) collecting a semen sample video, and uploading the video to a cloud; 2) performing frame extraction on the video to form an image, and performing pre-processing; 3) identifying information in the image; 4) tracking the sperms in the image according to the identification result; 5) analyzing and calculating a tracking trajectory result; and 6) scoring according to a calculation result, and outputting seminal fluid quality evaluation. Through a video monitoring function of a microscope camera, a semen video is recorded and uploaded to a cloud end (computer equipment does not need to be arranged through cloud end design), the video is subjected to picture interception and analysis through an AI algorithm, each sperm in the semen video is identified and tracked, a tracking result is analyzed and scored, and the sperm tracking accuracy is improved. And finally, giving out the activity score of the seminal fluid.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for influencing the linear motion / far-end mass droplet character of a pig sperm. The SNP molecular marker is Ggt (Glutathione Ggt) which is positioned at the 31908556 bp position of the 10th chromosome of a pig genome Ensembl Sscrofa11.1 version; a mutation. By verifying the influence effect of the molecular marker on linear motion and far-end mass droplet characters of pig sperms, an efficient and accurate molecular marker assisted breeding technology is finally established, and the molecular marker assisted breeding technology is applied to genetic improvement of boars for improving the quality of the boar sperms, so that the reproductive performance of offspring pigs is improved, the economic profit of enterprises is increased, and the core competitiveness is increased. By optimizing the dominant allele A of the SNP, the frequency of the dominant allele A can be increased generation by generation, the semen quality of the boars can be improved, excellent boars with the two characters can be bred cooperatively, the genetic improvement progress of the boars can be accelerated, and the economic benefit of boar breeding can be effectively improved.

The invention discloses a bull semen quality related SNP molecular marker based on a CCDC89 gene and application of the bull semen quality related SNP molecular marker, and belongs to the technical field of genetic molecular biology, the SNP molecular marker is located at the position of chr29: 9868636 of the bull CCDC89 gene, the gene polymorphism is G or A, and when the SNP site is G, high semen quality is shown. By detecting the polymorphism of the SNP site, the detection of the bull semen quality and the identification of the reproductive performance can be realized, the breeding is assisted, the reliable support is provided for breeding the next generation of high quality, and the SNP site has the advantages of high reliability, high efficiency, low cost and the like.

This invention relates to the field of beauty and skincare, and discloses an automated system and method for detecting semen quality, comprising: a morphological defect rate calculation module for calculating the morphological defect rate corresponding to a target semen sample; a dysfunctional sperm identification module for identifying dysfunctional sperm in the target semen sample; a reproductive function scoring module for evaluating the reproductive function score corresponding to the target semen sample; a dynamic motility potential scoring module for configuring a suitable culture medium for the target semen sample, culturing the target semen sample in the suitable culture medium, recording the sperm viability baseline parameters during the culture process, calculating the sperm survival rate of the target semen sample, and evaluating the dynamic motility potential score corresponding to the target semen sample; and a semen quality analysis module for generating a semen quality analysis report corresponding to the target semen sample. This invention can improve the accuracy of semen quality detection.

The invention discloses a feed for improving the quality of semen of an old boar and an application of the feed. The feed disclosed by the invention is prepared from the following components in parts by weight: 8 to 10 parts of corn, 18 to 23 parts of red sorghum, 37 to 43 parts of hulled barley, 12 to 17 parts of bran, 0.5 to 1.5 parts of functional fiber, 7 to 9 parts of soybean meal, 1.5 to 2.5 parts of fish oil or microalgae powder, 1.3 to 1.4 parts of mountain flour, 1 to 1.2 parts of monocalcium phosphate, 0.4 to 0.5 part of sodium chloride, 0.2 to 0.3 part of lysine, 0.1 to 0.15 part of L-threonine, 0.1 to 0.15 part of choline chloride, 1 to 1.5 parts of betaine and 0.5 to 1 part of a premix for boars. After the feed is applied, the total effective sperm number of the aged boars is remarkably increased, the sperm motility and form are improved, the breeding age limit is prolonged, the production value is increased, and the biological safety risk caused by the high renewal rate is reduced.

The present invention discloses a semen quality prediction method and system based on random forests. The method comprises: obtaining metabolite data samples of different semens and preprocessing the metabolite data samples to obtain preprocessed metabolite data samples; analyzing the preprocessed metabolite data samples based on preset multidimensional screening criteria to determine differential features associated with abnormal semen quality; screening and evaluating the differential features using a preset feature screening algorithm, and determining a core feature subset that meets semen quality prediction requirements based on preset optimization indicators; training a pre-constructed semen quality prediction model based on a random forest algorithm based on the core feature subset; and using the trained semen quality prediction model to perform quality prediction on the metabolite data of the semen to be tested to obtain a semen quality prediction result. The present invention can improve the accuracy of semen quality prediction.

The invention discloses a method for predicting the sperm motility of a breeding bull based on a host SNP-rumen microorganism-metabolite interaction axis, and the sperm motility of the breeding bull can be obtained by detecting the haplotype condition of SNP1-SNP3 and / or SNP4-SNP6 sites of a breeding bull genome. The method breaks through the limitations of strong dependency on phenotype endpoint evaluation and prediction lag in the current bull seed selection process, and realizes forward accurate prediction and early evaluation of the semen quality of the breeding bull.

The present application belongs to the technical field of bull semen quality evaluation, and particularly relates to a local bull semen quality evaluation method and system based on multi-source data analysis. The method comprises: when real-time data corresponding to a current semen sample is acquired, executing a quality evaluation process to generate a comprehensive score of the current semen sample; based on the offset amplitude of sample data generated by the evaluation model in the benchmark test, determining a main parameter and at least one subsidiary parameter from a plurality of to-be-tested parameters associated with the current semen sample. The present application calculates a subsidiary score according to the subsidiary parameter, and adjusts the subsidiary score by using an external parameter to generate a comprehensive score; and a multi-dimensional dynamic evaluation framework is constructed to consider a plurality of to-be-tested parameters of the semen itself, and the external parameter is quantified and integrated into the final score, so that the evaluation result can dynamically adapt to different environments and individual conditions, and overcome the limitation of single evaluation dimension in the prior art.

The invention relates to the technical field of boar breeding, and discloses local boar breeding equipment which comprises a support. The supporting frame is arranged above the support and used for supporting local boars to take sperms; the lifting assembly is arranged between the support and the supporting frame; the protective plate is arranged on the arc-shaped outer wall of the supporting frame; the adjusting assembly is arranged on the outer wall of the protective plate and used for adjusting and supporting local breeding pigs; the semen taking unit is arranged in the supporting frame and is used for manually taking out local boar semen. Through assistance of height adjustment, angle adjustment and the massage assembly, a more comfortable and natural semen collection environment is provided for boars. The optimized semen collection environment can reduce the stress reaction of the boars and improve the semen quality, and the design of the flow guide assembly and the collection mechanism can effectively reduce the waste of the semen in the semen collection and collection process. Therefore, the utilization rate of the seminal fluid is improved, and the production cost is reduced.

The invention relates to the technical field of cock breeding, in particular to a biomarker, a preparation method and application thereof and a method for assisting in breeding cock semen quality identification. The biomarker comprises the following components: phosphatidylcholine, lysophosphatidylcholine, phosphatidyl ethanolamine, lysophosphatidyl ethanolamine, phosphatidylserine and lysophosphatidylserine. According to the invention, key markers can be rapidly detected from the semen so as to determine breeding suitable for liquid-state preservation of the semen and high-activity individuals. The artificial insemination interval time can be greatly prolonged, and long-time high-sperm active breeding cock individuals can be rapidly bred. The breeder cock breeding amount can be reduced, grains are saved, and breeding benefits are greatly improved.

The invention provides a molecular marker related to the freezing tolerance of pig sperms, and belongs to the field of pig genetic breeding, and the molecular marker related to the freezing tolerance of the pig sperms is named as g.21555606Ggt; a is located at a nucleotide site of 21555606bp of a pig No.18 chromosome of a reference sequence of an international pig genome version 11.1, the basic group of the site is G or A, and g.21555606Ggt; a is located in an intron region of the GRM8 gene and corresponds to the 192bp position in a nucleotide sequence shown in SEQ ID NO: 1. The dominant genotype boar individual has better frozen sperm quality, so that g.21555606Ggt, g.21555606Ggt, g.215556Ggt The A is applied to genetic breeding of pigs and can assist in improving the frozen semen quality of breeding boars.

The application provides a pig semen quality rapid analysis instrument and relates to the field of reproductive experiment test platforms. The pig semen quality rapid analysis instrument comprises a workbench, the one side of the tabletop of the workbench is provided with an up-down through-type placing groove, comprises an electric sliding component, is installed on the placing groove and can independently slide forward and backward, comprises a constant-temperature storage component, the electric sliding component can be linked to open the constant-temperature storage component when moving forward, comprises a convenient cleaning and sterilization component, is fixedly installed on the electric sliding component. The pig semen quality rapid analysis instrument can spray and disinfect the handheld sampling equipment, can perform immersion cleaning when heavy cleaning, both forms are relatively fast, especially the hand flushing disinfection and drying for reuse, improve the reuse efficiency of the semen sampling analysis instrument, and manual use of alcohol is not needed, the operator's hand does not leave the equipment all the time, the probability that the equipment is contaminated is greatly reduced, and therefore the experimental analysis result obtained is more accurate.

The invention relates to the technical field of frozen sperms, in particular to a cattle sex control frozen sperm preservation method which comprises the following steps: collecting bull semen, and purifying the bull semen to obtain a sperm suspension so as to determine the qualification of the quality of the semen; performing sperm sorting on the sperm suspension by using a flow cytometry to separate target sperms based on a determination result that the quality of the sperm is qualified; uniformly mixing the target sperms with a refrigerating fluid, standing for a preset period of time, and freezing; obtaining a particle size distribution curve and an ROS concentration curve of the target sperm in the freezing process, and determining the qualification of the temperature drop rate according to the distribution proportion of the particle size distribution curve; adjusting the temperature drop rate according to a comparison result of an ROS fluctuation index of the ROS concentration curve and a preset index on the basis of a judgment result that the temperature drop rate is unqualified; and unfreezing and resuscitating the frozen target sperms to determine the sperm malformation rate so as to adjust the preset activity or the preset index. According to the invention, the quality of frozen sperms is improved.

The invention belongs to the technical field of animal breeding, and particularly relates to application of alpha-ketoglutaric acid or salt thereof in preparation of a sheep semen cryopreservation diluent. The invention discloses the application of alpha-ketoglutaric acid or salt thereof in the cryopreservation of the sheep semen for the first time, and the alpha-ketoglutaric acid or salt thereof can improve the semen quality of the frozen and unfrozen sheep semen and improve the fertilization ability of the frozen semen; such as sperm motility, sperm movement performance, sperm viability, sperm plasma membrane integrity rate, sperm acrosome integrity rate, sperm antioxidant capacity, sperm ATP level, sperm early apoptosis incidence rate, sperm in-vitro fertilized ovulation rate and / or artificial fertilization pregnancy rate, can be used for preparing the sheep sperm cryopreservation diluent.

The invention discloses a chicken seminal fluid quality judgment device and application. The chicken seminal fluid quality can be quickly and accurately judged on site. The method comprises the following steps: collecting a seminal fluid sample of a cock, detecting seminal fluid quality indexes, namely forward movement and linear speed, through an iSperm instrument, and substituting the seminal fluid quality indexes into a Fisher discriminant function: y = 0.126 * 1-0.069 * 2-7.425 (x1: forward movement; x2: linear velocity); if y is less than 0, determining that the semen quality is high, and if y is greater than 0, determining that the semen quality is low. And high-quality breeder cocks are quickly screened for mating, so that the hatching egg fertilization rate is increased.

The invention provides a traditional Chinese medicine composition with kidney tonifying and essence strengthening functions and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine. The traditional Chinese medicine composition with the kidney tonifying and essence strengthening functions is prepared from the following raw materials in parts by weight: 8 to 22 parts of antler slices, 5 to 15 parts of prepared radix aconiti lateralis preparata slices, 8 to 23 parts of herba epimedii, 5 to 15 parts of radix morindae officinalis, 5 to 15 parts of herba cistanche, 8 to 22 parts of radix rehmanniae preparata, 8 to 22 parts of tribulus terrestris, 5 to 15 parts of turtle shells, 8 to 22 parts of tortoise shells, 5 to 15 parts of radix angelicae sinensis, 8 to 22 parts of fructus lycii, 3 to 9 parts of flos caryophylli, 15 to 45 parts of radix astragali seu hedysari, 5 to 15 parts of rhizoma polygonati and 3 to 7 parts of sun-dried ginseng. The traditional Chinese medicine composition is decocted with water to obtain a decoction, after the traditional Chinese medicine composition is administrated to a patient with oligospermia or asthenospermia, the semen quality of the patient can be effectively improved, the sperm density is improved, the sperm motility rate and the normal sperm form proportion are increased, and the problem of male infertility is solved.

The invention discloses application of pseudomonas as a marker in evaluation of bull semen quality, and belongs to the technical field of animal breeding and microbiomics. The relative abundance of Pseudomonas in a bull rumen fluid with high semen quality is significantly higher than the relative abundance in a bull rumen fluid with low semen quality. By detecting the relative abundance, the bull semen quality can be evaluated, and the reproductive performance can be reliably judged on the premise of not depending on a semen sample. Evaluation can be carried out before semen collection for the first time or in a breeding candidate period, potential low-fecundity individuals are screened out in advance, and the breeding risk is reduced.

The invention discloses a method for recovering sperm production of breeder cocks without / with weak sperm, and belongs to the technical field of poultry breeding. The method comprises the following steps: collecting seminal fluid of healthy cocks of more than 28 weeks old for two consecutive weeks, and selecting non-seminal breeder cocks; two weeks before fasting, the screened breeding cocks are transferred to a closed henhouse, and stress relieving medicine is added into drinking water; feeding and water supply are cut off from the first day to the third day from fasting, illumination is adjusted, and water supply is recovered from the fourth day; when the weight loss rate of the breeder cock group reaches 25-30%, gradually recovering feeding and illumination, and if the death rate in the implementation period exceeds 5%, stopping hunger, and recovering feeding and illumination; and observing the health condition of the chicken flocks every day, detecting whether the breeding cocks generate sperms or not 15 days after the breeding cocks recover to eat, and monitoring the body weight at the same time. The invention provides a method for recovering sperm production of breeder cocks without / with weak sperms, which can solve the problem that the hatching rate is influenced due to poor sperm quality or no sperm of the breeder cocks without / with weak sperms in the seed production.

The invention provides application of exogenous D-ribose in improving the normal-temperature preservation effect of boar semen and a method for improving the normal-temperature preservation effect of the boar semen. According to the technology disclosed by the invention, D-ribose is added into the boar semen stored at normal temperature, so that the effects of enhancing the oxidation resistance of the semen, improving the sperm motility and improving the quality of the semen can be achieved, and particularly, indexes such as the sperm motility, the plasma membrane integrity rate, T-AOC, CAT, GSH-Px and SOD are obviously higher than those of all groups including a control group; the ROS content of the sperms is obviously lower than that of all other groups.

The invention belongs to the technical field of livestock breeding, and discloses composite insect powder, composite insect powder zymolyte and a feed additive for improving the quality of breeding male poultry semen. The composite insect powder is tenebrio molitor powder, hermetia illucens powder and cricket powder. Furthermore, the composite insect powder is adopted for enzymolysis, and the effect after enzymolysis is better. After the composite insect powder and the composite insect powder zymolyte are used as feed additives to be added into breeding male poultry feed for feeding, core reproductive performance indexes such as sperm motility and sperm density can be remarkably improved, the sperm motility can reach 86% or above, the sperm density can reach 4 * 10 < 9 > / mL or above, and the breeding male poultry can be fed with the composite insect powder zymolyte. And the compound insect powder has obvious multiple compound effects of yellow mealworms, hermetia illucens and crickets. The compound insect powder is used as a feed additive, the breeding male poultry semen quality is comprehensively improved through multi-dimensional indexes, the raw materials are easy to obtain, use is easy and convenient, and the compound insect powder feed is especially suitable for popularization of large-scale breeding poultry breeding.

The invention discloses a method for rapidly constructing a fluorescent array sensor and application of the fluorescent array sensor, and the method comprises the following steps: through four-component Ugi chemical reaction, synthesizing substituted amide fluorescent small molecules by using a carboxyl chemical substance, an amino chemical substance, a ketone or aldehyde chemical substance and an isonitrile compound which are equal in molar weight to construct an element library; and screening out a plurality of elements with the maximum contribution through a machine learning algorithm to quickly and efficiently construct the array sensor. The fluorescent array sensor disclosed by the invention can simultaneously screen healthy sperm samples and sperm samples of different types of diseases, the to-be-screened sperm sample can be cryopreserved for a long time and does not need to be used after being taken, the sperm sample of a to-be-diagnosed person can be collected at home, and the embarrassment of taking the sperm sample from a hospital toilet is avoided. Meanwhile, the array sensor has the advantages of high sensitivity, short detection time, low cost, good repeatability, high accuracy, no need of professional technicians and the like, and semen quality problems caused by different factors can be quickly identified within one minute.

The invention discloses a method for improving inhibition of slow heat stress on male rabbit breeding, which is executed by a computer system and comprises the following steps: acquiring environmental data and physiological indexes such as male rabbit ear temperature and respiratory rate in real time through a multi-modal sensor; the core algorithm constructs a slow heat stress cumulative damage model containing a time decay factor, and quantifies the integral effect of the heat load and the body repair process. The system generates multi-dimensional optimal control vectors of environment cooling, nutrition intervention and LED red light repair through a physiology-environment coordinated regulation and control model on the basis of an accumulated damage index generated by the model. According to the method, the limitation of traditional single threshold value control is broken through, and full-period precise prevention and control of physical cooling open circuit, nutrition restoration follow-up and illumination rehabilitation ending is achieved. Experiments prove that the scheme can effectively protect testis tissues, shorten the reproductive function recovery period and improve the semen quality, the breeding and medicament cost is reduced while the reproductive performance is guaranteed, and the method is suitable for different scales of rabbit farms.

The invention relates to the technical field of livestock breeding, in particular to a special lick brick for relieving heat stress and improving bull semen quality as well as a preparation method and application of the special lick brick. According to the special lick brick, on the premise that basic mineral supply is not changed, through synergistic addition of astaxanthin and betaine, key semen quality indexes such as the number of sperms, sperm motility and the like of Holstein breeding bulls advancing linearly are pertinently improved. Meanwhile, through the synergistic effect of astaxanthin and betaine, the oxidation resistance of the body can be remarkably enhanced, the heat stress related index level is reduced, and inhibition of heat stress on reproductive performance is relieved. The preparation method provided by the invention is simple and convenient in process, convenient to use and controllable in cost, not only can meet the requirement of large-scale breeding for practicability, but also can indirectly improve the breeding economic benefit by improving the semen quality, is suitable for daily breeding of 1-4-year-old Holstein breeding bulls, and has wide popularization and application prospects.

The present invention provides a molecular marker identification method related to pig semen quality and its application, relating to the field of biotechnology. This application involves applying a substance for detecting the SNP molecular marker rs3471304372 to the preparation of a product for predicting pig semen quality. The SNP molecular marker rs3471304372 is associated with pig sperm motility and sperm density, with the TT genotype being the dominant genotype. Breeding pigs with the TT genotype can effectively improve pig sperm motility and sperm density. Using this genetic marker to assess Large White pig semen quality and select boars is of great significance for pig genetic breeding.

The invention relates to the technical field of animal breeding, in particular to a product for improving the spermatogenic function of testis of a boar under heat stress and application of the product. According to the invention, a Rongchang boar under heat stress is taken as a model, the NAD + level in dynamic testis can be effectively improved by supplementing 30mg / kg BW / d of nicotinamide nucleoside, and the decrease of the NAD + level in the testis caused by heat stress is improved. In addition, the invention also finds that by supplementing 30mg / kg BW / d of nicotinamide riboside, the semen quality can be improved, the spermatogenesis defect can be reduced, and oxidative stress and inflammatory response caused by heat stress can be alleviated. Therefore, supplement of the NAD + precursor plays an important role in relieving testis injury of the boar under heat stress and improving semen quality.

The invention broadly relates to methods for marker assisted identification and / or selection of subjects having one or more genetic markers associated with a phenotype of interest. More particularly, the invention relates to methods of marker assisted identification and / or selection or rejection of non-human subjects, and particularly bovine subjects, for one or more traits associated with productivity and / or worth, for example fertility such as sperm and / or semen quality, by determining the presence of one or polymorphisms associated with the one or more productivity and / or worth traits.

A composition for use in a method of improving semen quality of a human male, said composition comprising at least one spermidine source, and optionally one or more further components and / or one or more carriers or excipients.

The invention discloses application of methyl nicotinate as a marker in evaluation of bull semen quality, and belongs to the technical field of animal reproduction and metabonomics. The relative content of methyl nicotinate in the bull rumen fluid with high semen quality is significantly higher than the relative content of methyl nicotinate in the bull rumen fluid with low semen quality. By detecting the relative content, the bull semen quality is evaluated, the reproductive performance can be reliably judged on the premise of not depending on a semen sample, and the method has the remarkable advantages of being non-invasive, high in throughput, capable of advancing a prediction window and the like.

The utility model relates to the technical field of semen quality analysis, in particular to a concentric circle sperm quality analyzer sample pretreatment structure which comprises a monitor, the bottom end of the inner side of the monitor is fixedly connected with a stepping motor, and the output end of the stepping motor is fixedly connected with a connecting disc. A first circular ring, a second circular ring and a third circular ring are fixedly connected to the top of the connecting disc, an insertion hole is formed in the first circular ring, a sampling cup is slidably connected to the insertion hole, a fixing cylinder is fixedly connected to the bottom end of the inner side of the insertion hole, and placement grooves are symmetrically formed in the outer side of the fixing cylinder; the concentric circle sperm quality analyzer sample pretreatment structure comprises a fixing cylinder, a placing groove is formed in the fixing cylinder, a mounting disc is fixedly connected to the inner side of the fixing cylinder, a connecting groove is formed in the mounting disc, and the interior of the placing groove is communicated with the interior of the connecting groove. And sampling cups with different sizes can be quickly clamped and fixed.