44 results about "Semen quality" patented technology

The invention discloses an automatic grading and grouping system and method for boars, and relates to the technical field of boar breeding, and the system constructs a multi-modal biological characteristic data set by collecting gene, semen quality, health examination and growth performance data. Secondly, extracting gene features by using a convolutional neural network, calculating a gene screening coefficient JYX, and classifying the gene screening coefficient JYX into an A class level and a B class level through a first threshold Q1; then, semen quality analysis is conducted on the A-class boars, a semen quality screening coefficient ZLX is calculated, and the A-class boars are divided into the A1-class boars and the A2-class boars through a second threshold Q2; and when the A2-class boar triggers health early warning, further evaluating a health index JCZ, and subdividing the health index JCZ into A21, A22 and A23 classes according to a third threshold Q3. And finally, grouping the boars according to the class levels of the boars, and formulating an accurate feeding strategy to optimize the reproductive performance and improve the breeding benefits.

The invention discloses a bull semen quality related SNP molecular marker based on a CCDC89 gene and application of the bull semen quality related SNP molecular marker, and belongs to the technical field of genetic molecular biology, the SNP molecular marker is located at the position of chr29: 9868636 of the bull CCDC89 gene, the gene polymorphism is G or A, and when the SNP site is G, high semen quality is shown. By detecting the polymorphism of the SNP site, the detection of the bull semen quality and the identification of the reproductive performance can be realized, the breeding is assisted, the reliable support is provided for breeding the next generation of high quality, and the SNP site has the advantages of high reliability, high efficiency, low cost and the like.

This invention relates to the field of beauty and skincare, and discloses an automated system and method for detecting semen quality, comprising: a morphological defect rate calculation module for calculating the morphological defect rate corresponding to a target semen sample; a dysfunctional sperm identification module for identifying dysfunctional sperm in the target semen sample; a reproductive function scoring module for evaluating the reproductive function score corresponding to the target semen sample; a dynamic motility potential scoring module for configuring a suitable culture medium for the target semen sample, culturing the target semen sample in the suitable culture medium, recording the sperm viability baseline parameters during the culture process, calculating the sperm survival rate of the target semen sample, and evaluating the dynamic motility potential score corresponding to the target semen sample; and a semen quality analysis module for generating a semen quality analysis report corresponding to the target semen sample. This invention can improve the accuracy of semen quality detection.

The invention discloses a feed for improving the quality of semen of an old boar and an application of the feed. The feed disclosed by the invention is prepared from the following components in parts by weight: 8 to 10 parts of corn, 18 to 23 parts of red sorghum, 37 to 43 parts of hulled barley, 12 to 17 parts of bran, 0.5 to 1.5 parts of functional fiber, 7 to 9 parts of soybean meal, 1.5 to 2.5 parts of fish oil or microalgae powder, 1.3 to 1.4 parts of mountain flour, 1 to 1.2 parts of monocalcium phosphate, 0.4 to 0.5 part of sodium chloride, 0.2 to 0.3 part of lysine, 0.1 to 0.15 part of L-threonine, 0.1 to 0.15 part of choline chloride, 1 to 1.5 parts of betaine and 0.5 to 1 part of a premix for boars. After the feed is applied, the total effective sperm number of the aged boars is remarkably increased, the sperm motility and form are improved, the breeding age limit is prolonged, the production value is increased, and the biological safety risk caused by the high renewal rate is reduced.

The present invention discloses a semen quality prediction method and system based on random forests. The method comprises: obtaining metabolite data samples of different semens and preprocessing the metabolite data samples to obtain preprocessed metabolite data samples; analyzing the preprocessed metabolite data samples based on preset multidimensional screening criteria to determine differential features associated with abnormal semen quality; screening and evaluating the differential features using a preset feature screening algorithm, and determining a core feature subset that meets semen quality prediction requirements based on preset optimization indicators; training a pre-constructed semen quality prediction model based on a random forest algorithm based on the core feature subset; and using the trained semen quality prediction model to perform quality prediction on the metabolite data of the semen to be tested to obtain a semen quality prediction result. The present invention can improve the accuracy of semen quality prediction.

The invention discloses a method for predicting the sperm motility of a breeding bull based on a host SNP-rumen microorganism-metabolite interaction axis, and the sperm motility of the breeding bull can be obtained by detecting the haplotype condition of SNP1-SNP3 and / or SNP4-SNP6 sites of a breeding bull genome. The method breaks through the limitations of strong dependency on phenotype endpoint evaluation and prediction lag in the current bull seed selection process, and realizes forward accurate prediction and early evaluation of the semen quality of the breeding bull.

The present application belongs to the technical field of bull semen quality evaluation, and particularly relates to a local bull semen quality evaluation method and system based on multi-source data analysis. The method comprises: when real-time data corresponding to a current semen sample is acquired, executing a quality evaluation process to generate a comprehensive score of the current semen sample; based on the offset amplitude of sample data generated by the evaluation model in the benchmark test, determining a main parameter and at least one subsidiary parameter from a plurality of to-be-tested parameters associated with the current semen sample. The present application calculates a subsidiary score according to the subsidiary parameter, and adjusts the subsidiary score by using an external parameter to generate a comprehensive score; and a multi-dimensional dynamic evaluation framework is constructed to consider a plurality of to-be-tested parameters of the semen itself, and the external parameter is quantified and integrated into the final score, so that the evaluation result can dynamically adapt to different environments and individual conditions, and overcome the limitation of single evaluation dimension in the prior art.

The invention relates to the technical field of boar breeding, and discloses local boar breeding equipment which comprises a support. The supporting frame is arranged above the support and used for supporting local boars to take sperms; the lifting assembly is arranged between the support and the supporting frame; the protective plate is arranged on the arc-shaped outer wall of the supporting frame; the adjusting assembly is arranged on the outer wall of the protective plate and used for adjusting and supporting local breeding pigs; the semen taking unit is arranged in the supporting frame and is used for manually taking out local boar semen. Through assistance of height adjustment, angle adjustment and the massage assembly, a more comfortable and natural semen collection environment is provided for boars. The optimized semen collection environment can reduce the stress reaction of the boars and improve the semen quality, and the design of the flow guide assembly and the collection mechanism can effectively reduce the waste of the semen in the semen collection and collection process. Therefore, the utilization rate of the seminal fluid is improved, and the production cost is reduced.

The invention relates to the technical field of cock breeding, in particular to a biomarker, a preparation method and application thereof and a method for assisting in breeding cock semen quality identification. The biomarker comprises the following components: phosphatidylcholine, lysophosphatidylcholine, phosphatidyl ethanolamine, lysophosphatidyl ethanolamine, phosphatidylserine and lysophosphatidylserine. According to the invention, key markers can be rapidly detected from the semen so as to determine breeding suitable for liquid-state preservation of the semen and high-activity individuals. The artificial insemination interval time can be greatly prolonged, and long-time high-sperm active breeding cock individuals can be rapidly bred. The breeder cock breeding amount can be reduced, grains are saved, and breeding benefits are greatly improved.

The application provides a pig semen quality rapid analysis instrument and relates to the field of reproductive experiment test platforms. The pig semen quality rapid analysis instrument comprises a workbench, the one side of the tabletop of the workbench is provided with an up-down through-type placing groove, comprises an electric sliding component, is installed on the placing groove and can independently slide forward and backward, comprises a constant-temperature storage component, the electric sliding component can be linked to open the constant-temperature storage component when moving forward, comprises a convenient cleaning and sterilization component, is fixedly installed on the electric sliding component. The pig semen quality rapid analysis instrument can spray and disinfect the handheld sampling equipment, can perform immersion cleaning when heavy cleaning, both forms are relatively fast, especially the hand flushing disinfection and drying for reuse, improve the reuse efficiency of the semen sampling analysis instrument, and manual use of alcohol is not needed, the operator's hand does not leave the equipment all the time, the probability that the equipment is contaminated is greatly reduced, and therefore the experimental analysis result obtained is more accurate.

The invention relates to the technical field of frozen sperms, in particular to a cattle sex control frozen sperm preservation method which comprises the following steps: collecting bull semen, and purifying the bull semen to obtain a sperm suspension so as to determine the qualification of the quality of the semen; performing sperm sorting on the sperm suspension by using a flow cytometry to separate target sperms based on a determination result that the quality of the sperm is qualified; uniformly mixing the target sperms with a refrigerating fluid, standing for a preset period of time, and freezing; obtaining a particle size distribution curve and an ROS concentration curve of the target sperm in the freezing process, and determining the qualification of the temperature drop rate according to the distribution proportion of the particle size distribution curve; adjusting the temperature drop rate according to a comparison result of an ROS fluctuation index of the ROS concentration curve and a preset index on the basis of a judgment result that the temperature drop rate is unqualified; and unfreezing and resuscitating the frozen target sperms to determine the sperm malformation rate so as to adjust the preset activity or the preset index. According to the invention, the quality of frozen sperms is improved.

The invention belongs to the technical field of animal breeding, and particularly relates to application of alpha-ketoglutaric acid or salt thereof in preparation of a sheep semen cryopreservation diluent. The invention discloses the application of alpha-ketoglutaric acid or salt thereof in the cryopreservation of the sheep semen for the first time, and the alpha-ketoglutaric acid or salt thereof can improve the semen quality of the frozen and unfrozen sheep semen and improve the fertilization ability of the frozen semen; such as sperm motility, sperm movement performance, sperm viability, sperm plasma membrane integrity rate, sperm acrosome integrity rate, sperm antioxidant capacity, sperm ATP level, sperm early apoptosis incidence rate, sperm in-vitro fertilized ovulation rate and / or artificial fertilization pregnancy rate, can be used for preparing the sheep sperm cryopreservation diluent.

The invention provides a traditional Chinese medicine composition with kidney tonifying and essence strengthening functions and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine. The traditional Chinese medicine composition with the kidney tonifying and essence strengthening functions is prepared from the following raw materials in parts by weight: 8 to 22 parts of antler slices, 5 to 15 parts of prepared radix aconiti lateralis preparata slices, 8 to 23 parts of herba epimedii, 5 to 15 parts of radix morindae officinalis, 5 to 15 parts of herba cistanche, 8 to 22 parts of radix rehmanniae preparata, 8 to 22 parts of tribulus terrestris, 5 to 15 parts of turtle shells, 8 to 22 parts of tortoise shells, 5 to 15 parts of radix angelicae sinensis, 8 to 22 parts of fructus lycii, 3 to 9 parts of flos caryophylli, 15 to 45 parts of radix astragali seu hedysari, 5 to 15 parts of rhizoma polygonati and 3 to 7 parts of sun-dried ginseng. The traditional Chinese medicine composition is decocted with water to obtain a decoction, after the traditional Chinese medicine composition is administrated to a patient with oligospermia or asthenospermia, the semen quality of the patient can be effectively improved, the sperm density is improved, the sperm motility rate and the normal sperm form proportion are increased, and the problem of male infertility is solved.

The invention discloses application of pseudomonas as a marker in evaluation of bull semen quality, and belongs to the technical field of animal breeding and microbiomics. The relative abundance of Pseudomonas in a bull rumen fluid with high semen quality is significantly higher than the relative abundance in a bull rumen fluid with low semen quality. By detecting the relative abundance, the bull semen quality can be evaluated, and the reproductive performance can be reliably judged on the premise of not depending on a semen sample. Evaluation can be carried out before semen collection for the first time or in a breeding candidate period, potential low-fecundity individuals are screened out in advance, and the breeding risk is reduced.

The invention provides application of exogenous D-ribose in improving the normal-temperature preservation effect of boar semen and a method for improving the normal-temperature preservation effect of the boar semen. According to the technology disclosed by the invention, D-ribose is added into the boar semen stored at normal temperature, so that the effects of enhancing the oxidation resistance of the semen, improving the sperm motility and improving the quality of the semen can be achieved, and particularly, indexes such as the sperm motility, the plasma membrane integrity rate, T-AOC, CAT, GSH-Px and SOD are obviously higher than those of all groups including a control group; the ROS content of the sperms is obviously lower than that of all other groups.

The invention belongs to the technical field of livestock breeding, and discloses composite insect powder, composite insect powder zymolyte and a feed additive for improving the quality of breeding male poultry semen. The composite insect powder is tenebrio molitor powder, hermetia illucens powder and cricket powder. Furthermore, the composite insect powder is adopted for enzymolysis, and the effect after enzymolysis is better. After the composite insect powder and the composite insect powder zymolyte are used as feed additives to be added into breeding male poultry feed for feeding, core reproductive performance indexes such as sperm motility and sperm density can be remarkably improved, the sperm motility can reach 86% or above, the sperm density can reach 4 * 10 < 9 > / mL or above, and the breeding male poultry can be fed with the composite insect powder zymolyte. And the compound insect powder has obvious multiple compound effects of yellow mealworms, hermetia illucens and crickets. The compound insect powder is used as a feed additive, the breeding male poultry semen quality is comprehensively improved through multi-dimensional indexes, the raw materials are easy to obtain, use is easy and convenient, and the compound insect powder feed is especially suitable for popularization of large-scale breeding poultry breeding.

The invention discloses a method for improving inhibition of slow heat stress on male rabbit breeding, which is executed by a computer system and comprises the following steps: acquiring environmental data and physiological indexes such as male rabbit ear temperature and respiratory rate in real time through a multi-modal sensor; the core algorithm constructs a slow heat stress cumulative damage model containing a time decay factor, and quantifies the integral effect of the heat load and the body repair process. The system generates multi-dimensional optimal control vectors of environment cooling, nutrition intervention and LED red light repair through a physiology-environment coordinated regulation and control model on the basis of an accumulated damage index generated by the model. According to the method, the limitation of traditional single threshold value control is broken through, and full-period precise prevention and control of physical cooling open circuit, nutrition restoration follow-up and illumination rehabilitation ending is achieved. Experiments prove that the scheme can effectively protect testis tissues, shorten the reproductive function recovery period and improve the semen quality, the breeding and medicament cost is reduced while the reproductive performance is guaranteed, and the method is suitable for different scales of rabbit farms.

The invention relates to the technical field of livestock breeding, in particular to a special lick brick for relieving heat stress and improving bull semen quality as well as a preparation method and application of the special lick brick. According to the special lick brick, on the premise that basic mineral supply is not changed, through synergistic addition of astaxanthin and betaine, key semen quality indexes such as the number of sperms, sperm motility and the like of Holstein breeding bulls advancing linearly are pertinently improved. Meanwhile, through the synergistic effect of astaxanthin and betaine, the oxidation resistance of the body can be remarkably enhanced, the heat stress related index level is reduced, and inhibition of heat stress on reproductive performance is relieved. The preparation method provided by the invention is simple and convenient in process, convenient to use and controllable in cost, not only can meet the requirement of large-scale breeding for practicability, but also can indirectly improve the breeding economic benefit by improving the semen quality, is suitable for daily breeding of 1-4-year-old Holstein breeding bulls, and has wide popularization and application prospects.

The present invention provides a molecular marker identification method related to pig semen quality and its application, relating to the field of biotechnology. This application involves applying a substance for detecting the SNP molecular marker rs3471304372 to the preparation of a product for predicting pig semen quality. The SNP molecular marker rs3471304372 is associated with pig sperm motility and sperm density, with the TT genotype being the dominant genotype. Breeding pigs with the TT genotype can effectively improve pig sperm motility and sperm density. Using this genetic marker to assess Large White pig semen quality and select boars is of great significance for pig genetic breeding.

The invention relates to the technical field of animal breeding, in particular to a product for improving the spermatogenic function of testis of a boar under heat stress and application of the product. According to the invention, a Rongchang boar under heat stress is taken as a model, the NAD + level in dynamic testis can be effectively improved by supplementing 30mg / kg BW / d of nicotinamide nucleoside, and the decrease of the NAD + level in the testis caused by heat stress is improved. In addition, the invention also finds that by supplementing 30mg / kg BW / d of nicotinamide riboside, the semen quality can be improved, the spermatogenesis defect can be reduced, and oxidative stress and inflammatory response caused by heat stress can be alleviated. Therefore, supplement of the NAD + precursor plays an important role in relieving testis injury of the boar under heat stress and improving semen quality.

The invention broadly relates to methods for marker assisted identification and / or selection of subjects having one or more genetic markers associated with a phenotype of interest. More particularly, the invention relates to methods of marker assisted identification and / or selection or rejection of non-human subjects, and particularly bovine subjects, for one or more traits associated with productivity and / or worth, for example fertility such as sperm and / or semen quality, by determining the presence of one or polymorphisms associated with the one or more productivity and / or worth traits.

The invention discloses application of methyl nicotinate as a marker in evaluation of bull semen quality, and belongs to the technical field of animal reproduction and metabonomics. The relative content of methyl nicotinate in the bull rumen fluid with high semen quality is significantly higher than the relative content of methyl nicotinate in the bull rumen fluid with low semen quality. By detecting the relative content, the bull semen quality is evaluated, the reproductive performance can be reliably judged on the premise of not depending on a semen sample, and the method has the remarkable advantages of being non-invasive, high in throughput, capable of advancing a prediction window and the like.

The utility model relates to the technical field of semen quality analysis, in particular to a concentric circle sperm quality analyzer sample pretreatment structure which comprises a monitor, the bottom end of the inner side of the monitor is fixedly connected with a stepping motor, and the output end of the stepping motor is fixedly connected with a connecting disc. A first circular ring, a second circular ring and a third circular ring are fixedly connected to the top of the connecting disc, an insertion hole is formed in the first circular ring, a sampling cup is slidably connected to the insertion hole, a fixing cylinder is fixedly connected to the bottom end of the inner side of the insertion hole, and placement grooves are symmetrically formed in the outer side of the fixing cylinder; the concentric circle sperm quality analyzer sample pretreatment structure comprises a fixing cylinder, a placing groove is formed in the fixing cylinder, a mounting disc is fixedly connected to the inner side of the fixing cylinder, a connecting groove is formed in the mounting disc, and the interior of the placing groove is communicated with the interior of the connecting groove. And sampling cups with different sizes can be quickly clamped and fixed.

The invention discloses application of spermidine in preparation of a medicine for improving semen quality of a heat stress substance. The spermidine is adopted to improve the sperm motility of a heat stress agonist and relieve testis injury, is an important substance required in the normal physiological process of mammals, and widely participates in the processes of cell growth, proliferation, differentiation and the like, so that the influence of heat stress on germ cells is relieved by utilizing the spermidine, and the effect of preventing and treating testis injury is achieved. The potential side effect is small or even no theoretically, and the production and application value is high. According to the invention, spermidine is adopted, so that sperm movement parameters of a heat stress mouse can be improved, the integrity of testis tissues is obviously recovered, the repair of a seminiferous tubule structure is promoted, and cell layer arrangement tends to be normal, which indicates that spermidine can relieve mouse testis dysfunction caused by heat stress.

The invention belongs to the technical field of medicines, and particularly relates to application of a Chinese chive seed extract in preparation of a medicine for treating oligoasthenospermia. Based on network pharmacology prediction and animal experiment verification, it is found that by activating a PI3K / AKT signal channel, testicular cell apoptosis is inhibited, spermatogenesis disorder is improved, and therefore the sperm number and vitality of an oligoasthenospermia model rat are remarkably improved. Clinical treatment research in combination with levocarnitine oral liquid proves that the traditional Chinese medicine composition can reduce the sperm DFI level and improve the semen quality. Animal research proves that the compound can regulate the level of serum testosterone (T), reduce the levels of follicle stimulating hormone (FSH) and luteinizing hormone (LH), repair pathological damage of testis tissues, and antagonize testis cell apoptosis induced by tripterygium glycosides by up-regulating protein expression of p-PI3K / PI3K, p-AKT / AKT and Bcl-2.

The invention discloses a boar seminal fluid quality rapid analysis instrument, and belongs to the technical field of boar seminal fluid quality analysis, the boar seminal fluid quality rapid analysis instrument comprises an analyzer body, an analysis end used for quality analysis is arranged on one side in the analyzer body, and an analysis table is arranged below the analysis end. According to the device, along with the continuous downward movement of the mounting seat, the mounting seat can drive the mounting sleeve, the placement barrel and a semen sample in the placement barrel to slowly and uniformly rotate through the sliding sleeve, the guide rod and the spiral chute, so that the semen sample in the placement barrel is subjected to auxiliary mixing, and the precipitation phenomenon in the semen sample is reduced; according to the multi-station multi-station seminal fluid sampling device, layering errors are effectively avoided, the consistency of seminal fluid in samples is improved, the influence on the quality analysis precision of the device is further prevented, the device supports batch sample queue processing through multi-station design, the single sample turnover time is shortened, automatic process standardized operation is achieved, the operation consistency is guaranteed, and the data comparability is effectively improved.

The invention relates to the field of beauty and skin care, and discloses a system and method for automatically detecting semen quality, and the system comprises a form defect rate calculation module which is used for calculating the form defect rate corresponding to a target semen sample; the dysfunctional sperm identification module is used for identifying dysfunctional sperms in the target sperm sample; the reproductive function score evaluation module is used for evaluating a reproductive function score corresponding to the target semen sample; and the dynamic activity potential scoring evaluation module is used for preparing an adaptive culture solution corresponding to the target semen sample, putting the target semen sample into the adaptive culture solution for culture treatment, recording sperm motility rate reference parameters in the culture treatment process, and calculating the sperm survival rate of the target semen sample. Evaluating a dynamic activity potential score corresponding to the target semen sample; and the semen quality analysis module is used for generating a semen quality analysis report corresponding to the target semen sample. The detection accuracy of the semen quality can be improved.

The utility model relates to a sperm freezing descent control device which comprises a liquid nitrogen tank used for containing liquid nitrogen, a pedestal and a controller, hollow horizontal rods are arranged on the pedestal, a winding wheel is rotatably connected between the inner side walls of the left ends of the horizontal rods, a fixed pulley is rotatably connected between the inner side walls of the right ends of the horizontal rods, and the fixed pulley is connected with the controller. A pull rope is wound on the winding wheel, and the free end of the pull rope naturally droops after bypassing the fixed pulley and is detachably connected with a hanging basket used for containing a freezing thin tube; a motor electrically connected with the controller is arranged at the left end of the horizontal rod, the output end of the motor is fixedly connected with the winding wheel, a distance sensor used for monitoring the height between the hanging basket and the liquid level of liquid nitrogen in the liquid nitrogen tank is arranged on the hanging basket, and the distance sensor is in signal connection with the controller. Different liquid nitrogen gas phase pre-freezing heights can be adjusted according to different semen qualities, so that the semen is ensured to be at a proper pre-freezing temperature, the semen freezing quality is improved, and the inactivation rate is reduced.

The invention discloses a detection method and application for frozen bee semen quality, and the detection method is characterized in that the state, sperm motility, DNA integrity and the like of unfrozen bee semen are used as detection indexes of frozen bee semen quality, and the detection indexes are detected and graded in multiple dimensions; and the quality condition of the frozen bee semen can be reflected more comprehensively and accurately. The detection method is especially designed according to the characteristics of small head and long body of the bee sperm, the process is small in sperm damage, simple and easy to operate, short in detection time, low in cost and suitable for being popularized in the field.

The application relates to the technical field of semen quality evaluation, and discloses a semen quality evaluation method based on deep learning, which comprises the following operation steps: S1, pre-processing a data set, selecting data of 85 male participants aged 18 or above, the data containing a video of a live sperm sample of each participant, using 60% of the data set for training, using 20% as a verification set, using 20% as a test set, pre-processing the training set video, and taking 9 continuous frames as a sample input. The semen quality evaluation method based on deep learning directly extracts video information of the semen video by using a light-weight MobileNet, improves the convolutional neural network, adds other data in addition to the video information, increases the prediction accuracy of the network, deploys the neural network by using a front-end and back-end separated Web end, solves the prediction efficiency problem, and makes the whole system better in operation and better in displaying the prediction result.