74 results about "Sperm quality" patented technology

The invention relates to the technical field of biomedicine detection reagent manufacturing and reproductive medicine, in particular to an embryonic development potential sperm key protein kit and a use method thereof. The method is characterized in that an antibody combination capable of specifically recognizing a group of key proteins related to embryonic development potential in human sperms is prepared, and the antibodies, a fluorescence labeled secondary antibody, a cell nucleus dye, a concentrated washing liquid, a mounting medium and a pre-coated control porous glass slide are assembled into the special detection kit. The kit can be combined with a multi-mode imaging system (such as a fluorescence / confocal microscope and a common optical microscope) and image analysis software to realize the traditional analysis of sperm morphology and movement ability, and can perform synchronous and accurate qualitative and quantitative analysis with the expression level and subcellular localization of the key protein. The invention provides an integrated molecular detection solution, and provides a powerful tool for sperm quality screening and embryonic development potential prediction in an assisted reproduction technology.

The invention provides an application of ergothioneine as a testis oxidative stress regulator. The invention provides a multi-target intervention method based on ergothioneine, which systematically improves sperm quality reduction caused by obesity by cooperatively regulating key links such as oxidative stress level, hormone secretion state and mitochondrial function. According to the invention, a safe and effective intervention effect with a clear action mechanism can be realized, and a new technical approach is provided for the treatment of obesity-related male reproductive dysfunction. The invention also provides application of the ergothioneine as the testicular steroid generation accelerant.

1. Name of the product in this design: Sperm Quality Analyzer. 2. Purpose of this design: The product is intended for use by research institutions and medical institutions for sperm concentration detection, motility analysis, sperm morphology identification, and determination of semen pH and DNA fragmentation rate in semen samples. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: 3D rendering 2.

The invention relates to the technical field of traditional Chinese medicine, in particular to a medicine for improving sperm quality and application thereof.The medicine is reasonably combined according to etiology and pathogenesis of male infertility with abnormal sperm quality, prepared rehmannia root and poria cocos serve as monarch medicine, cistanche, astragalus membranaceus and ginseng serve as ministerial medicine, maca serves as adjuvant medicine, kidney tonifying is assisted, and the effect of improving the sperm quality is achieved. The traditional Chinese medicine composition has the effects of tonifying kidney, replenishing essence, promoting diuresis, dissolving turbidity and treating spleen and kidney through compatibility and synergism of all the medicines, improves abnormal sperm quality, improves sperm quality, effectively treats male infertility and can take both symptoms and root causes into consideration.

This invention discloses a sperm quality detection sensor and its preparation method, belonging to the field of detection and sensing technology. It includes an electrode with an HA functional layer modified on its surface, the HA functional layer having amino active sites; a redox probe that generates an electrical signal with the electrode; and a conductive layer disposed between the electrode and the redox probe for conduction between them. This application specifically binds the HA functional layer to receptors on the surface of healthy sperm in the test semen and employs microcurrent signal processing technology. The specific binding of the HA functional layer to the receptors affects the current between the electrode and the redox probe. The change in current of the sperm quality detection sensor characterizes the number of healthy sperm in the test semen. This sperm quality detection sensor overcomes the limitations of morphological interpretation in traditional detection technologies, significantly reducing the size of sperm detection equipment and improving the accuracy of sperm quality detection.

The application discloses an information processing method and device for detecting sperm quality, equipment and medium, applied to a computer device, relates to the field of biomedical detection, and comprises the following steps: injecting an original semen sample into a preset microfluidic chip to dilute the original semen sample, and collecting multi-modal diluted sample data; performing parallel feature analysis on the multi-modal diluted sample data to determine corresponding multiple sperm characteristics; performing feature fusion on the multiple sperm characteristics based on a preset feature weight distribution network to obtain sperm fusion characteristics; determining corresponding several sperm quality parameters based on the sperm fusion characteristics, and determining the sperm quality of the original semen sample based on the several sperm quality parameters. Therefore, the detection efficiency and result reliability can be improved through highly automated synchronous multi-modal data acquisition and deep fusion analysis.

The invention discloses a preparation for improving male erectile function and sperm quality. The health-care food is characterized by being prepared from the following raw materials in parts by weight: 30-50 parts of herba epimedii extract, 20-40 parts of herba cistanche extract, 15-30 parts of fructus lycii extract, 18-35 parts of semen cuscutae extract, 10-25 parts of herba cynomorii extract, 8-20 parts of maca powder, 6-18 parts of raspberry extract, 0.5-2 parts of lycopene, 1-3 parts of L-carnitine and 0.3-1 part of vitamin B family (vitamin B1, vitamin B2, vitamin B6 and vitamin B12 are mixed according to the mass ratio of 1: 1: 2: 0.5). The herba epimedii extract is selected as a main component, the content of icariin is regulated to be not lower than 60%, the high-content icariin can more effectively promote sex hormone secretion, enhance sexual desire and improve erectile function, the total content of echinacoside and verbascoside in the herba cistanche extract is not lower than 45%, and the herba epimedii extract and the herba cistanche extract have the effect of promoting sex hormone secretion, enhancing sexual desire and improving erectile function. The two components have the effects of tonifying kidney yang and benefiting essence and blood, and can enhance kidney functions.

To provide a sperm selection aid that enables the selection of good quality sperm suitable for intracytoplasmic sperm injection (ICSI) from among the sperm in a sample. [Solution] A sperm sorting assistance device comprising: an index calculation unit that calculates at least one index related to the quality of sperm recognized in a video; an evaluation unit that relatively evaluates the quality of first sperm recognized in a first time interval in a video and second sperm recognized in a second time interval different from the first time interval in a video, based on at least one index; and a display control unit that displays information relating to at least one index of the first sperm to the first sperm in the first time interval in a video, and displays the relative quality evaluation of the first sperm and the second sperm evaluated by the evaluation unit.

The application provides a molecular marker related to the freezing tolerance of pig sperm, belongs to the field of pig genetic breeding, and is named g.21555606G>A, is located at a nucleotide site of 21555606bp of pig chromosome 18 in an international pig genome 11.1 version reference sequence, the base of the site is G or A, the g.21555606G>A is located in an intron region of a GRM8 gene, and corresponds to the 192th bp in a nucleotide sequence shown in SEQ ID NO:1. A superior genotype boar individual has better frozen sperm quality, so that the g.21555606G>A is applied to the genetic breeding of pigs, and the frozen sperm quality of a boar can be improved.

1. The name of the design product: full-automatic sperm quality analyzer (CASA OMNI). 2. The use of the design product: the design product is used for testing and data analysis of the dynamics, morphology, DNA fragments, survival and leukocyte conditions of biological semen and sperm. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view 1.

The invention discloses a method for evaluating damage of ionizing radiation to male sperm quality, which belongs to the technical field of reproductive biology, and comprises the following steps: establishing a dose-effect database model between ionizing radiation dose and male sperm quality based on a logistic regression model, determining a mapping relation between sperm quality rating and fertility recovery average time; collecting a to-be-detected semen sample exposed by ionizing radiation, comparing the to-be-detected semen sample with the dose-effect database model, evaluating the male sperm quality rating, and predicting the fertility recovery time based on the male sperm quality rating and the mapping relation.

This makes it possible to select high-quality sperm suitable for intracytoplasmic sperm injection (ICSI) from among the sperm in a sample. To provide a sperm sorting assistance device. [Solution] Calculate at least one indicator related to sperm quality recognized in the video. An index calculation unit and, based on at least one index, during a first time interval in the video The first sperm recognized, and in a second time interval different from the first time interval in the video image An evaluation unit that relatively evaluates the quality of the second sperm recognized by the first time in the video During the interval, information regarding at least one indicator of the first sperm for the first sperm. The relationship between the first sperm and the second sperm, as evaluated by the evaluation unit, is displayed. A sperm sorting assistance device comprising a display control unit that displays an evaluation of relative quality.

The application discloses a Holstein cow sperm activity miRNA molecular marker selection method, which comprises the following steps: (1) specific primers are designed according to a Holstein cow miRNA precursor region, and PCR amplification is carried out on to-be-detected cow genomic DNA to obtain an amplification product; (2) the PCR product is subjected to enzyme cutting by using a BspE I restriction endonuclease, and electrophoresis typing is carried out, and three genotypes, namely, a TT type, a TC type and a CC type, are obtained; and (3) the sperm activity of the CC type is significantly higher than that of the TT type, and the sperm deformity rate of the CC type is significantly lower than that of the TT type, so the CC type and the TC type are selected for breeding, and the C genotype frequency is improved. The method can improve the accuracy of sperm quality selection of the cow, is used for the selection and breeding of high-quality cows, and provides a selection and breeding tool for strengthening the excellent germplasm of Chinese Holstein cows.

The invention relates to the field of microorganisms, and particularly discloses a clostridium butyricum microecological preparation composition for improving the sperm quality of livestock and poultry. The preparation method comprises the following steps: selecting materials such as cortex phellodendri to prepare mixed powder, using materials such as zinc-rich edible yeast powder to prepare a pasty bacterial colony proliferation culture medium, using materials such as the mixed powder and clostridium butyricum dry powder to prepare activated clostridium butyricum bacterial sludge, and performing mixed fermentation on the activated clostridium butyricum bacterial sludge and the pasty bacterial colony proliferation culture medium; and finally, pouring a mixed solution formed by xanthan gum, fructo-oligosaccharide and purified water into mixed dry powder of materials such as the bacteria-rich fermentation mixture, L-carnitine and the like to obtain a blend wet material, and drying the blend wet material to obtain the microecological preparation composition. The microecological preparation composition is added into the feed, so that the palatability and the nutrient utilization degree of the feed can be effectively improved, the ingestion and digestion of livestock and poultry are promoted, the organism immunity of the livestock and poultry is enhanced, and the semen quality of the livestock and poultry is improved on the premise of avoiding overdraft of the livestock and poultry.

Disclosed are methods and products for improving the quality of sperm, methods for treating subjects to improve sperm quality or improve fertility, and use of sperm with improved quality in assisted reproductive technologies, including exposing the sperm to BGP-15 and / or a derivative thereof, such as BGP-15, propanolol, bimoclomol, arimoclomal, NG94, iroxanadine, and / or a pharmaceutically acceptable derivative, prodrug, solvate, salt, tautomer, stereoisomer, and / or racemate thereof.

The invention discloses a method for improving the quality of male sperms. Belongs to the technical field of biological medicine. It is found that the sperm quality of male mice can be remarkably improved by long-term in-vivo application of D-mannose. The method is mainly embodied in that kinematics parameters of total sperm motility rate and forward motility rate are remarkably increased, and the effect is independent of gonad development improvement; if the sperm mitochondrial membrane potential rises, the energy metabolism function is improved; the sperm acrosome integrity is improved, and the sperm quality is integrally optimized. The results show that D-mannose, as a safe natural monosaccharide, can significantly improve the quality of male sperms in an in-vivo intervention manner, and provides a new method and approach for treatment and intervention of male infertility, especially asthenozoospermia.

The utility model discloses a sperm quality detection and analysis device. The sperm quality detection and analysis device comprises a cabinet body, a plurality of storage grooves are formed in the cabinet body, a pair of vertical positioning plates are fixedly connected into the side walls of the storage grooves, a plurality of supporting plate assemblies are slidably connected to the end faces of one sides of the vertical positioning plates, and a detection module is slidably connected to the end faces of one sides of the supporting plate assemblies. A plurality of first grooves are formed in the end face of one side of the supporting plate assembly, limiting assemblies matched with the detection modules are slidably connected into the groove walls of the first grooves, a plurality of second grooves are formed in the end face of one side of the containing groove, and ejection assemblies matched with the detection modules are slidably connected into the side walls of the second grooves. Compared with the prior art, the sperm quality detection and analysis device disclosed by the utility model has the advantages that the detection modules can be conveniently mounted and dismounted, and the detection modules can be conveniently increased or decreased as required.

Stabilized amorphous calcium carbonate (ACC) for treatment of several neurological, muscular and infertility diseases and conditions is provided. In particular, the stabilized ACC may be used in the treatment of axonal defects and muscular dystrophy. In addition, provided are improved methods used in assistant reproductive technology. Examples of such methods are in vitro fertilization and improvement of sperm quality. The improved IVF method, for example, comprises addition of the stabilized ACC to the cell culture medium in which the stages of fertilization and embryo development occurs.

The application relates to the technical field of livestock reproduction, and particularly discloses a sperm microcapsule preparation and a preparation method; the sperm microcapsule preparation comprises a base liquid, carboxylated fullerene and sodium alginate; the base liquid comprises a sperm diluent, an amikacin mixed solution and egg yolk; the concentration of the carboxylated fullerene is 0-18 mu g / mL; and the concentration of the sodium alginate is 1%-2%. The application can reduce the damage of the microencapsulation process to sperm and improve the sperm quality in the sperm incubation process.

The invention relates to the technical field of health-care food, in particular to a sea cucumber peptide solid beverage capable of enhancing immunity and a production process of the sea cucumber peptide solid beverage. The sea cucumber peptide health-care food is prepared from the following raw materials: more than or equal to 50% of sea cucumber peptide, more than or equal to 10.55% of rhizoma polygonati powder, more than or equal to 5% of fructus lycii powder, more than or equal to 5% of mulberry powder, more than or equal to 4% of herba cistanche powder, more than or equal to 1% of eucommia male pollen, more than or equal to 1% of gynura procumbens powder, more than or equal to 1% of ginseng powder, more than or equal to 0.5% of maca powder and Through scientific compatibility of the sea cucumber peptide, the rhizoma polygonati, the herba cistanche and other components, the content of lactic acid and malondialdehyde can be reduced, the testosterone level of serum and the sperm quality can be remarkably improved, deep synergy of fatigue resistance, oxidation resistance and improvement of male physiological indexes is achieved, the small molecule characteristic that the high-content sea cucumber peptide is easily absorbed by intestinal tracts is utilized, and the health care effect is achieved. And in cooperation with protein and rich B vitamins and minerals, the quick conversion requirement of organisms on high-concentration nutrients is effectively met.

The present disclosure provides a sperm sorting assistance device that enables selection of high-quality sperm suitable for intracytoplasmic sperm injection from among sperm in a specimen. The present disclosure provides a sperm sorting assistance device comprising: an index calculation unit that calculates at least one index relating to the quality of sperm recognized in a moving image; an evaluation unit that, on the basis of the at least one index, relatively evaluates the quality of first sperm recognized during a first time interval in the moving image and second sperm recognized during a second time interval different from the first time interval in the moving image; and a display control unit that, in the first time interval in the moving image, displays information relating to the at least one index of the first sperm in association with the first sperm, and displays an evaluation of the relative quality of the first sperm and the second sperm evaluated by the evaluation unit.

The utility model relates to the technical field of semen mixing equipment, in particular to semen mixing equipment for a full-automatic sperm quality analyzer, which comprises a bottom plate, a first support column, a second support column, a stepping motor and a rotating shaft, a first supporting column is arranged on one side of the top face of the bottom plate, and a second supporting column is arranged on the other side. One end of the rotating shaft is rotationally connected with the first supporting column, and the other end is rotationally connected with the second supporting column; a mounting hole for placing a centrifugal tube is formed in the rotating shaft; a stepping motor used for driving the rotating shaft to rotate is arranged on the first supporting column. The stepping motor drives the rotating shaft to rotate, and the rotating shaft drives the centrifugal tube to rotate, so that semen in the centrifugal tube is uniformly mixed; the rotating speed of the stepping motor is relatively uniform, and the stepping motor drives the centrifugal tube to rotate, so that the problem of non-uniform sample distribution caused by manual uniform mixing can be avoided.

The invention discloses a traditional Chinese medicine formula with a reproductive system radiation damage protection effect as well as a preparation method and application of the traditional Chinese medicine formula. The traditional Chinese medicine formula comprises the following components in parts by mass: 15-30 parts of Chinese yam, 6-15 parts of dogwood, 10-30 parts of astragalus membranaceus, 10-30 parts of prepared rehmannia root, 10-15 parts of spina date seed, 6-15 parts of fructus psoraleae, 5-15 parts of fructus lycii and 3-9 parts of rhodiola rosea. In the traditional Chinese medicine formula provided by the invention, all the components have a synergistic effect, so that the injury of ionizing radiation to a reproductive system can be effectively relieved, the pathological change of testis tissues is remarkably improved, sperm quality parameters are improved, the apoptosis process of testis cells is inhibited, the injury caused by oxidative stress is repaired, and the DNA injury degree is reduced; therefore, the technical defects of high toxicity, high cost, insufficient protective effect on the reproductive system and the like of the existing radiation protective agent are overcome.

The application provides application of a SNP genetic marker in a PPP3CA gene related to freezing tolerance of pig sperm in pig genetic breeding, and belongs to the technical field of pig genetic marker detection; the SNP genetic marker in the PPP3CA gene related to freezing tolerance of pig sperm is named g.119750378A>C, is located at a nucleotide site of 119750378bp of pig chromosome 8 in an international pig genome 11.1 version reference sequence, the base of the site is A or C, the g.119750378A>C is located in an intron region of the PPP3CA gene, and corresponds to the 174th bp in a nucleotide sequence shown in SEQ ID NO:1. A boar individual with an advantageous genotype AA has better frozen sperm quality, so that the g.119750378A>C is applied to genetic breeding of pigs, and freezing quality of a boar can be improved from a gene level.

The invention discloses a plant lactobacillus, a metagen and application of the plant lactobacillus and the metagen for improving the activity and quality of male sperms, the plant lactobacillus is plant lactobacillus BN04, the plant lactobacillus is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 22, 2023, and the preservation number is CGMCC No.29102. The invention further discloses a preparation method of the plant lactobacillus and the metagen for improving the activity and quality of male sperms and application of the plant lactobacillus and the metagen for improving the activity and quality of male sperms. The plant lactobacillus BN04 provided by the invention can regulate the copper content in seminal plasma to a normal level and improve the sperm motility and quality, and the metagen prepared from the plant lactobacillus BN04 can enhance the acrosin activity of sperms and improve the sperm quality.

The invention discloses a nutrient composition for improving the male fertility function and a preparation method and application of the nutrient composition. The composition is composed of zinc, selenium, coenzyme Q10, L-acetyl carnitine, folic acid, vitamin D3, natural lycopene, vitamin E, L-arginine and core components such as standardized herba epimedii, fructus lycii and maca extract according to a specific ratio, and three pairs of key mass ratios of zinc / selenium, L-acetyl carnitine / coenzyme Q10 and natural lycopene / vitamin E are limited for the first time. The composition can synergistically improve sperm quality, reduce oxidative stress and improve serum testosterone level in a multi-target manner, the invention further provides a preparation method of the composition, the uniformity and stability of the composition are ensured through the processes of graded mixing, low-temperature sterilization and the like, and the composition can be used for preparing dietary supplements or health care products for improving oligospermia, asthenozoospermia and abnormal spermatozoospermia.

This invention relates to the fields of biomedical diagnostic reagent manufacturing and reproductive medicine technology, and particularly to a detection system and method for assessing sperm motility in patients with asthenospermia. This invention establishes a complete, accurate, and efficient molecular-level sperm quality evaluation system by integrating specific fluorescent labeling, high-resolution confocal imaging, and automated computer analysis software. This system not only overcomes the limitations of existing technologies but also further expands its functional scope by integrating an optical microscopy analysis module. It possesses good clinical applicability and scalability, providing a powerful tool for the etiological diagnosis, efficacy evaluation, and scientific research of male infertility, and has significant clinical application value and market prospects.

The invention relates to a compound microbial agent and application thereof, belongs to the technical field of microorganisms, and particularly discloses application of mixed fermented milk in a product for improving male functions. According to the application, the new application of the mixed fermented milk of the rhamnosus lactobacillus B6 and the streptococcus thermophilus XJ53 (CGMCC No.17323) in the aspect of improving male functions is disclosed for the first time, and the new application is specifically characterized by improving male fertility desire, reproductive capacity, seminiferous tubule dysfunction and spermatogenesis, testis oxidative stress activity, testis testosterone synthesis and sperm quality; the strain has a potential value of producing a gain substance for improving the male function by fermenting skimmed milk, and also prompts that the fermented milk has a potential application prospect for enhancing the male function.

The invention relates to the technical field of environmental medicine and reproductive toxicology, in particular to application of aspirin in prevention and / or treatment of male reproductive injury caused by rare earth elements. The invention also relates to a method for improving sperm quality reduction caused by rare earth element exposure. The method comprises the step of administering an effective dose of aspirin or a pharmaceutically acceptable salt thereof to an individual in need thereof. The invention also relates to a pharmaceutical composition for preventing and / or treating male reproductive injury caused by rare earth elements. The cognitive limitation of a traditional oxidative stress mechanism is broken through, a new mechanism of rare earth reproductive toxicity is disclosed from a new perspective of testis immune microenvironment for the first time, and an intervention scheme aiming at the new mechanism is provided. According to the invention, the aspirin is creatively used for solving the novel public health problem of low-dose long-term exposure of rare earth elements for the first time, and a new application field is developed for non-steroidal anti-inflammatory drugs.

The invention belongs to the technical field of microorganisms, and particularly discloses application of lactobacillus rhamnosus fermented milk in a product for improving male functions. According to the application, the new application of the lactobacillus rhamnosus B6 (CGMCC No.13310) fermented milk in the aspect of improving male functions is disclosed for the first time, and the new application is specifically characterized by improving male fertility desire, reproductive capacity, seminiferous tubule dysfunction and spermatogenesis, testis oxidative stress activity, testis testosterone synthesis and sperm quality; the strain has a potential value of producing a gain substance for improving the male function by fermenting skimmed milk, and also prompts that the fermented milk has a potential application prospect for enhancing the male function.